CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/231.mrc Output diagnostic file name Micrographs/231.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/231.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2078.000 9928.000 Mean, RMS : 4317.911 222.7202 TITLE 1: emi2mrc: 231.emi Sun Jan 19 21:49:53 2014 TITLE 2: kV 300, Mag 78000x, Def 20670, Spot 4, Int 55.12%, Obj 88.08%, Dif 73.67% TITLE 3: X 118.877, Y 686.176, Z -36.397, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.02218 8200.00 8000.00 0.00 -0.02213 8400.00 8000.00 0.00 -0.02184 8600.00 8000.00 0.00 -0.02148 8800.00 8000.00 0.00 -0.02118 9000.00 8000.00 0.00 -0.02095 9200.00 8000.00 0.00 -0.02074 9400.00 8000.00 0.00 -0.02049 9600.00 8000.00 0.00 -0.02013 9800.00 8000.00 0.00 -0.01964 10000.00 8000.00 0.00 -0.01908 10200.00 8000.00 0.00 -0.01853 10400.00 8000.00 0.00 -0.01800 10600.00 8000.00 0.00 -0.01753 10800.00 8000.00 0.00 -0.01709 11000.00 8000.00 0.00 -0.01659 11200.00 8000.00 0.00 -0.01598 11400.00 8000.00 0.00 -0.01522 11600.00 8000.00 0.00 -0.01427 11800.00 8000.00 0.00 -0.01314 12000.00 8000.00 0.00 -0.01187 12200.00 8000.00 0.00 -0.01050 12400.00 8000.00 0.00 -0.00910 12600.00 8000.00 0.00 -0.00770 12800.00 8000.00 0.00 -0.00630 13000.00 8000.00 0.00 -0.00489 13200.00 8000.00 0.00 -0.00346 13400.00 8000.00 0.00 -0.00201 13600.00 8000.00 0.00 -0.00052 13800.00 8000.00 0.00 0.00101 14000.00 8000.00 0.00 0.00260 14200.00 8000.00 0.00 0.00427 14400.00 8000.00 0.00 0.00602 14600.00 8000.00 0.00 0.00790 14800.00 8000.00 0.00 0.00993 15000.00 8000.00 0.00 0.01213 15200.00 8000.00 0.00 0.01447 15400.00 8000.00 0.00 0.01683 15600.00 8000.00 0.00 0.01912 15800.00 8000.00 0.00 0.02126 16000.00 8000.00 0.00 0.02330 16200.00 8000.00 0.00 0.02538 16400.00 8000.00 0.00 0.02767 16600.00 8000.00 0.00 0.03025 16800.00 8000.00 0.00 0.03304 17000.00 8000.00 0.00 0.03592 17200.00 8000.00 0.00 0.03872 17400.00 8000.00 0.00 0.04142 17600.00 8000.00 0.00 0.04414 17800.00 8000.00 0.00 0.04699 18000.00 8000.00 0.00 0.05005 18200.00 8000.00 0.00 0.05324 18400.00 8000.00 0.00 0.05645 18600.00 8000.00 0.00 0.05961 18800.00 8000.00 0.00 0.06279 19000.00 8000.00 0.00 0.06614 19200.00 8000.00 0.00 0.06974 19400.00 8000.00 0.00 0.07351 19600.00 8000.00 0.00 0.07715 19800.00 8000.00 0.00 0.08028 20000.00 8000.00 0.00 0.08264 20200.00 8000.00 0.00 0.08419 20400.00 8000.00 0.00 0.08504 20600.00 8000.00 0.00 0.08544 20800.00 8000.00 0.00 0.08558 20400.00 8200.00 0.00 0.08589 20600.00 8200.00 0.00 0.08632 20800.00 8200.00 0.00 0.08647 20400.00 8400.00 0.00 0.08673 20600.00 8400.00 0.00 0.08719 20800.00 8400.00 0.00 0.08737 20400.00 8600.00 0.00 0.08775 20600.00 8600.00 0.00 0.08825 20800.00 8600.00 0.00 0.08844 20400.00 8800.00 0.00 0.08897 20600.00 8800.00 0.00 0.08948 20800.00 8800.00 0.00 0.08968 20400.00 9000.00 0.00 0.09028 20600.00 9000.00 0.00 0.09080 20800.00 9000.00 0.00 0.09098 20400.00 9200.00 0.00 0.09163 20600.00 9200.00 0.00 0.09214 20800.00 9200.00 0.00 0.09228 20400.00 9400.00 0.00 0.09294 20600.00 9400.00 0.00 0.09341 20800.00 9400.00 0.00 0.09351 20400.00 9600.00 0.00 0.09418 20600.00 9600.00 0.00 0.09462 20800.00 9600.00 0.00 0.09466 20400.00 9800.00 0.00 0.09549 20600.00 9800.00 0.00 0.09589 20200.00 10000.00 0.00 0.09600 20400.00 10000.00 0.00 0.09696 20600.00 10000.00 0.00 0.09732 20200.00 10200.00 0.00 0.09755 20400.00 10200.00 0.00 0.09851 20600.00 10200.00 0.00 0.09885 20200.00 10400.00 0.00 0.09915 20400.00 10400.00 0.00 0.10010 20600.00 10400.00 0.00 0.10041 20200.00 10600.00 0.00 0.10074 20400.00 10600.00 0.00 0.10168 20600.00 10600.00 0.00 0.10196 20200.00 10800.00 0.00 0.10229 20400.00 10800.00 0.00 0.10321 20600.00 10800.00 0.00 0.10343 20200.00 11000.00 0.00 0.10387 20400.00 11000.00 0.00 0.10474 20600.00 11000.00 0.00 0.10491 20200.00 11200.00 0.00 0.10557 20400.00 11200.00 0.00 0.10638 20600.00 11200.00 0.00 0.10649 20200.00 11400.00 0.00 0.10733 20400.00 11400.00 0.00 0.10809 20600.00 11400.00 0.00 0.10814 20200.00 11600.00 0.00 0.10909 20400.00 11600.00 0.00 0.10979 20200.00 11800.00 0.00 0.11084 20400.00 11800.00 0.00 0.11149 20200.00 12000.00 0.00 0.11256 20400.00 12000.00 0.00 0.11315 20200.00 12200.00 0.00 0.11431 20400.00 12200.00 0.00 0.11486 20200.00 12400.00 0.00 0.11624 20400.00 12400.00 0.00 0.11676 20000.00 12600.00 0.00 0.11685 20200.00 12600.00 0.00 0.11832 20400.00 12600.00 0.00 0.11883 20000.00 12800.00 0.00 0.11894 20200.00 12800.00 0.00 0.12039 20400.00 12800.00 0.00 0.12088 20000.00 13000.00 0.00 0.12095 20200.00 13000.00 0.00 0.12239 20400.00 13000.00 0.00 0.12285 20200.00 13200.00 0.00 0.12423 20400.00 13200.00 0.00 0.12466 20200.00 13400.00 0.00 0.12593 20400.00 13400.00 0.00 0.12632 20000.00 13600.00 0.00 0.12637 20200.00 13600.00 0.00 0.12770 20400.00 13600.00 0.00 0.12806 20000.00 13800.00 0.00 0.12839 20200.00 13800.00 0.00 0.12969 20400.00 13800.00 0.00 0.13001 20000.00 14000.00 0.00 0.13053 20200.00 14000.00 0.00 0.13182 20400.00 14000.00 0.00 0.13210 20000.00 14200.00 0.00 0.13274 20200.00 14200.00 0.00 0.13402 20400.00 14200.00 0.00 0.13429 20000.00 14400.00 0.00 0.13500 20200.00 14400.00 0.00 0.13628 20400.00 14400.00 0.00 0.13653 20000.00 14600.00 0.00 0.13728 20200.00 14600.00 0.00 0.13856 20400.00 14600.00 0.00 0.13880 20000.00 14800.00 0.00 0.13971 20200.00 14800.00 0.00 0.14098 20400.00 14800.00 0.00 0.14121 20000.00 15000.00 0.00 0.14245 20200.00 15000.00 0.00 0.14370 20400.00 15000.00 0.00 0.14388 20000.00 15200.00 0.00 0.14536 20200.00 15200.00 0.00 0.14656 20400.00 15200.00 0.00 0.14667 20000.00 15400.00 0.00 0.14825 20200.00 15400.00 0.00 0.14938 20400.00 15400.00 0.00 0.14938 20000.00 15600.00 0.00 0.15104 20200.00 15600.00 0.00 0.15208 20000.00 15800.00 0.00 0.15364 20200.00 15800.00 0.00 0.15460 20000.00 16000.00 0.00 0.15614 20200.00 16000.00 0.00 0.15701 20000.00 16200.00 0.00 0.15881 20200.00 16200.00 0.00 0.15960 20000.00 16400.00 0.00 0.16173 20200.00 16400.00 0.00 0.16242 19800.00 16600.00 0.00 0.16264 20000.00 16600.00 0.00 0.16471 20200.00 16600.00 0.00 0.16529 19800.00 16800.00 0.00 0.16565 20000.00 16800.00 0.00 0.16762 20200.00 16800.00 0.00 0.16808 19800.00 17000.00 0.00 0.16851 20000.00 17000.00 0.00 0.17038 20200.00 17000.00 0.00 0.17070 19800.00 17200.00 0.00 0.17117 20000.00 17200.00 0.00 0.17291 20200.00 17200.00 0.00 0.17309 19800.00 17400.00 0.00 0.17384 20000.00 17400.00 0.00 0.17543 20200.00 17400.00 0.00 0.17544 19800.00 17600.00 0.00 0.17667 20000.00 17600.00 0.00 0.17809 19800.00 17800.00 0.00 0.17946 20000.00 17800.00 0.00 0.18070 19800.00 18000.00 0.00 0.18201 20000.00 18000.00 0.00 0.18307 19800.00 18200.00 0.00 0.18425 20000.00 18200.00 0.00 0.18514 19800.00 18400.00 0.00 0.18611 20000.00 18400.00 0.00 0.18687 19800.00 18600.00 0.00 0.18793 20000.00 18600.00 0.00 0.18855 19800.00 18800.00 0.00 0.19014 20000.00 18800.00 0.00 0.19060 19800.00 19000.00 0.00 0.19270 20000.00 19000.00 0.00 0.19292 19600.00 19200.00 0.00 0.19320 19800.00 19200.00 0.00 0.19524 19600.00 19400.00 0.00 0.19580 19800.00 19400.00 0.00 0.19749 19600.00 19600.00 0.00 0.19773 19800.00 19600.00 0.00 0.19896 19800.00 19800.00 0.00 0.19936 19600.00 20000.00 35.00 0.19938 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19687.69 19891.08 39.12 0.19956 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/231.ctf NX, NY, NZ: 360 360 1 MODE : real