CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/241.mrc Output diagnostic file name Micrographs/241.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/241.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2250.000 8166.000 Mean, RMS : 4435.517 225.3797 TITLE 1: emi2mrc: 241.emi Sun Jan 19 22:26:23 2014 TITLE 2: kV 300, Mag 78000x, Def 22480, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -487.188, Y 533.281, Z -23.067, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01644 9600.00 8000.00 0.00 -0.01624 9800.00 8000.00 0.00 -0.01599 10000.00 8000.00 0.00 -0.01580 10200.00 8000.00 0.00 -0.01568 10400.00 8000.00 0.00 -0.01554 10600.00 8000.00 0.00 -0.01535 10800.00 8000.00 0.00 -0.01505 11000.00 8000.00 0.00 -0.01462 11200.00 8000.00 0.00 -0.01410 11400.00 8000.00 0.00 -0.01349 11600.00 8000.00 0.00 -0.01275 11800.00 8000.00 0.00 -0.01188 12000.00 8000.00 0.00 -0.01089 12200.00 8000.00 0.00 -0.00984 12400.00 8000.00 0.00 -0.00880 12600.00 8000.00 0.00 -0.00781 12800.00 8000.00 0.00 -0.00684 13000.00 8000.00 0.00 -0.00583 13200.00 8000.00 0.00 -0.00473 13400.00 8000.00 0.00 -0.00351 13600.00 8000.00 0.00 -0.00218 13800.00 8000.00 0.00 -0.00076 14000.00 8000.00 0.00 0.00071 14200.00 8000.00 0.00 0.00222 14400.00 8000.00 0.00 0.00380 14600.00 8000.00 0.00 0.00544 14800.00 8000.00 0.00 0.00720 15000.00 8000.00 0.00 0.00911 15200.00 8000.00 0.00 0.01120 15400.00 8000.00 0.00 0.01346 15600.00 8000.00 0.00 0.01584 15800.00 8000.00 0.00 0.01827 16000.00 8000.00 0.00 0.02066 16200.00 8000.00 0.00 0.02298 16400.00 8000.00 0.00 0.02529 16600.00 8000.00 0.00 0.02763 16800.00 8000.00 0.00 0.03006 17000.00 8000.00 0.00 0.03256 17200.00 8000.00 0.00 0.03508 17400.00 8000.00 0.00 0.03756 17600.00 8000.00 0.00 0.04002 17800.00 8000.00 0.00 0.04252 18000.00 8000.00 0.00 0.04512 18200.00 8000.00 0.00 0.04789 18400.00 8000.00 0.00 0.05081 18600.00 8000.00 0.00 0.05385 18800.00 8000.00 0.00 0.05702 19000.00 8000.00 0.00 0.06037 19200.00 8000.00 0.00 0.06397 19400.00 8000.00 0.00 0.06781 19600.00 8000.00 0.00 0.07176 19800.00 8000.00 0.00 0.07552 20000.00 8000.00 0.00 0.07876 20200.00 8000.00 0.00 0.08121 20400.00 8000.00 0.00 0.08272 20600.00 8000.00 0.00 0.08339 20800.00 8000.00 0.00 0.08342 20400.00 8200.00 0.00 0.08351 20600.00 8200.00 0.00 0.08418 20800.00 8200.00 0.00 0.08421 20400.00 8400.00 0.00 0.08425 20600.00 8400.00 0.00 0.08493 20800.00 8400.00 0.00 0.08497 20400.00 8600.00 0.00 0.08517 20600.00 8600.00 0.00 0.08586 20800.00 8600.00 0.00 0.08589 20400.00 8800.00 0.00 0.08629 20600.00 8800.00 0.00 0.08698 20800.00 8800.00 0.00 0.08702 20400.00 9000.00 0.00 0.08750 20600.00 9000.00 0.00 0.08820 20800.00 9000.00 0.00 0.08823 20400.00 9200.00 0.00 0.08875 20600.00 9200.00 0.00 0.08944 20800.00 9200.00 0.00 0.08947 20400.00 9400.00 0.00 0.08996 20600.00 9400.00 0.00 0.09064 20800.00 9400.00 0.00 0.09065 20400.00 9600.00 0.00 0.09112 20600.00 9600.00 0.00 0.09178 20400.00 9800.00 0.00 0.09238 20600.00 9800.00 0.00 0.09301 20400.00 10000.00 0.00 0.09382 20600.00 10000.00 0.00 0.09443 20400.00 10200.00 0.00 0.09535 20600.00 10200.00 0.00 0.09595 20400.00 10400.00 0.00 0.09692 20600.00 10400.00 0.00 0.09751 20400.00 10600.00 0.00 0.09849 20600.00 10600.00 0.00 0.09907 20400.00 10800.00 0.00 0.10005 20600.00 10800.00 0.00 0.10063 20400.00 11000.00 0.00 0.10175 20600.00 11000.00 0.00 0.10233 20400.00 11200.00 0.00 0.10373 20600.00 11200.00 0.00 0.10430 20200.00 11400.00 0.00 0.10440 20400.00 11400.00 0.00 0.10592 20600.00 11400.00 0.00 0.10649 20200.00 11600.00 0.00 0.10667 20400.00 11600.00 0.00 0.10819 20600.00 11600.00 0.00 0.10874 20200.00 11800.00 0.00 0.10892 20400.00 11800.00 0.00 0.11042 20600.00 11800.00 0.00 0.11095 20200.00 12000.00 0.00 0.11104 20400.00 12000.00 0.00 0.11252 20600.00 12000.00 0.00 0.11301 20200.00 12200.00 0.00 0.11310 20400.00 12200.00 0.00 0.11455 20600.00 12200.00 0.00 0.11501 20200.00 12400.00 0.00 0.11531 20400.00 12400.00 0.00 0.11673 20600.00 12400.00 0.00 0.11715 20200.00 12600.00 0.00 0.11770 20400.00 12600.00 0.00 0.11909 20600.00 12600.00 0.00 0.11947 20200.00 12800.00 0.00 0.12017 20400.00 12800.00 0.00 0.12152 20600.00 12800.00 0.00 0.12186 20200.00 13000.00 0.00 0.12264 20400.00 13000.00 0.00 0.12395 20600.00 13000.00 0.00 0.12425 20200.00 13200.00 0.00 0.12500 20400.00 13200.00 0.00 0.12628 20600.00 13200.00 0.00 0.12653 20200.00 13400.00 0.00 0.12725 20400.00 13400.00 0.00 0.12849 20600.00 13400.00 0.00 0.12871 20200.00 13600.00 0.00 0.12959 20400.00 13600.00 0.00 0.13081 20600.00 13600.00 0.00 0.13100 20200.00 13800.00 0.00 0.13217 20400.00 13800.00 0.00 0.13338 20600.00 13800.00 0.00 0.13353 20200.00 14000.00 0.00 0.13492 20400.00 14000.00 0.00 0.13610 20600.00 14000.00 0.00 0.13621 20200.00 14200.00 0.00 0.13773 20400.00 14200.00 0.00 0.13888 20600.00 14200.00 0.00 0.13894 20200.00 14400.00 0.00 0.14051 20400.00 14400.00 0.00 0.14162 20600.00 14400.00 0.00 0.14164 20200.00 14600.00 0.00 0.14315 20400.00 14600.00 0.00 0.14424 20200.00 14800.00 0.00 0.14580 20400.00 14800.00 0.00 0.14685 20200.00 15000.00 0.00 0.14867 20400.00 15000.00 0.00 0.14968 20200.00 15200.00 0.00 0.15172 20400.00 15200.00 0.00 0.15269 20200.00 15400.00 0.00 0.15485 20400.00 15400.00 0.00 0.15576 20000.00 15600.00 0.00 0.15576 20200.00 15600.00 0.00 0.15801 20400.00 15600.00 0.00 0.15886 20000.00 15800.00 0.00 0.15888 20200.00 15800.00 0.00 0.16108 20400.00 15800.00 0.00 0.16186 20000.00 16000.00 0.00 0.16194 20200.00 16000.00 0.00 0.16408 20400.00 16000.00 0.00 0.16479 20000.00 16200.00 0.00 0.16519 20200.00 16200.00 0.00 0.16727 20400.00 16200.00 0.00 0.16789 20000.00 16400.00 0.00 0.16870 20200.00 16400.00 0.00 0.17071 20400.00 16400.00 0.00 0.17124 20000.00 16600.00 0.00 0.17230 20200.00 16600.00 0.00 0.17423 20400.00 16600.00 0.00 0.17465 20000.00 16800.00 0.00 0.17591 20200.00 16800.00 0.00 0.17776 20400.00 16800.00 0.00 0.17807 20000.00 17000.00 0.00 0.17944 20200.00 17000.00 0.00 0.18121 20400.00 17000.00 0.00 0.18142 20000.00 17200.00 0.00 0.18286 20200.00 17200.00 0.00 0.18454 20400.00 17200.00 0.00 0.18464 20000.00 17400.00 0.00 0.18642 20200.00 17400.00 0.00 0.18801 20000.00 17600.00 0.00 0.19034 20200.00 17600.00 0.00 0.19180 20000.00 17800.00 0.00 0.19442 20200.00 17800.00 0.00 0.19573 20000.00 18000.00 0.00 0.19845 20200.00 18000.00 0.00 0.19958 20000.00 18200.00 0.00 0.20225 20200.00 18200.00 0.00 0.20320 20000.00 18400.00 0.00 0.20570 20200.00 18400.00 0.00 0.20645 19800.00 18600.00 0.00 0.20658 20000.00 18600.00 0.00 0.20906 20200.00 18600.00 0.00 0.20959 19800.00 18800.00 0.00 0.21059 20000.00 18800.00 0.00 0.21285 20200.00 18800.00 0.00 0.21309 19800.00 19000.00 0.00 0.21513 20000.00 19000.00 0.00 0.21706 19800.00 19200.00 0.00 0.21984 20000.00 19200.00 0.00 0.22131 19800.00 19400.00 0.00 0.22425 20000.00 19400.00 0.00 0.22513 19800.00 19600.00 0.00 0.22749 20000.00 19600.00 0.00 0.22771 19800.00 19800.00 0.00 0.22879 19800.00 20000.00 30.00 0.22885 19800.00 20000.00 35.00 0.22889 19800.00 20000.00 40.00 0.22891 19800.00 20000.00 45.00 0.22892 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19753.29 19929.21 50.88 0.22912 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/241.ctf NX, NY, NZ: 360 360 1 MODE : real