CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/242.mrc Output diagnostic file name Micrographs/242.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/242.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2392.000 8746.000 Mean, RMS : 4434.416 225.3886 TITLE 1: emi2mrc: 242.emi Sun Jan 19 22:27:14 2014 TITLE 2: kV 300, Mag 78000x, Def 22480, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -488.998, Y 531.326, Z -23.067, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01430 8200.00 8000.00 0.00 -0.01416 8400.00 8000.00 0.00 -0.01393 8600.00 8000.00 0.00 -0.01372 8800.00 8000.00 0.00 -0.01361 9000.00 8000.00 0.00 -0.01352 9200.00 8000.00 0.00 -0.01339 9400.00 8000.00 0.00 -0.01313 9600.00 8000.00 0.00 -0.01272 9800.00 8000.00 0.00 -0.01222 10000.00 8000.00 0.00 -0.01172 10200.00 8000.00 0.00 -0.01126 10400.00 8000.00 0.00 -0.01083 10600.00 8000.00 0.00 -0.01042 10800.00 8000.00 0.00 -0.00998 11000.00 8000.00 0.00 -0.00953 11200.00 8000.00 0.00 -0.00909 11400.00 8000.00 0.00 -0.00863 11600.00 8000.00 0.00 -0.00811 11800.00 8000.00 0.00 -0.00744 12000.00 8000.00 0.00 -0.00658 12200.00 8000.00 0.00 -0.00551 12400.00 8000.00 0.00 -0.00428 12600.00 8000.00 0.00 -0.00297 12800.00 8000.00 0.00 -0.00159 13000.00 8000.00 0.00 -0.00015 13200.00 8000.00 0.00 0.00139 13400.00 8000.00 0.00 0.00305 13600.00 8000.00 0.00 0.00482 13800.00 8000.00 0.00 0.00670 14000.00 8000.00 0.00 0.00869 14200.00 8000.00 0.00 0.01078 14400.00 8000.00 0.00 0.01297 14600.00 8000.00 0.00 0.01524 14800.00 8000.00 0.00 0.01755 15000.00 8000.00 0.00 0.01989 15200.00 8000.00 0.00 0.02227 15400.00 8000.00 0.00 0.02475 15600.00 8000.00 0.00 0.02740 15800.00 8000.00 0.00 0.03026 16000.00 8000.00 0.00 0.03324 16200.00 8000.00 0.00 0.03625 16400.00 8000.00 0.00 0.03918 16600.00 8000.00 0.00 0.04199 16800.00 8000.00 0.00 0.04470 17000.00 8000.00 0.00 0.04744 17200.00 8000.00 0.00 0.05028 17400.00 8000.00 0.00 0.05324 17600.00 8000.00 0.00 0.05631 17800.00 8000.00 0.00 0.05944 18000.00 8000.00 0.00 0.06263 18200.00 8000.00 0.00 0.06599 18400.00 8000.00 0.00 0.06960 18600.00 8000.00 0.00 0.07346 18800.00 8000.00 0.00 0.07747 19000.00 8000.00 0.00 0.08130 19200.00 8000.00 0.00 0.08457 19400.00 8000.00 0.00 0.08700 19600.00 8000.00 0.00 0.08849 19800.00 8000.00 0.00 0.08921 20000.00 8000.00 0.00 0.08946 20200.00 8000.00 0.00 0.08952 19800.00 8200.00 0.00 0.09013 20000.00 8200.00 0.00 0.09035 20200.00 8200.00 0.00 0.09038 19800.00 8400.00 0.00 0.09099 20000.00 8400.00 0.00 0.09120 20200.00 8400.00 0.00 0.09122 19600.00 8600.00 0.00 0.09132 19800.00 8600.00 0.00 0.09200 20000.00 8600.00 0.00 0.09220 20200.00 8600.00 0.00 0.09222 19600.00 8800.00 0.00 0.09252 19800.00 8800.00 0.00 0.09320 20000.00 8800.00 0.00 0.09340 20200.00 8800.00 0.00 0.09340 19600.00 9000.00 0.00 0.09382 19800.00 9000.00 0.00 0.09450 20000.00 9000.00 0.00 0.09469 19600.00 9200.00 0.00 0.09516 19800.00 9200.00 0.00 0.09584 20000.00 9200.00 0.00 0.09602 19600.00 9400.00 0.00 0.09648 19800.00 9400.00 0.00 0.09715 20000.00 9400.00 0.00 0.09732 19600.00 9600.00 0.00 0.09774 19800.00 9600.00 0.00 0.09842 20000.00 9600.00 0.00 0.09858 19600.00 9800.00 0.00 0.09913 19800.00 9800.00 0.00 0.09980 20000.00 9800.00 0.00 0.09995 19600.00 10000.00 0.00 0.10074 19800.00 10000.00 0.00 0.10142 20000.00 10000.00 0.00 0.10154 19600.00 10200.00 0.00 0.10256 19800.00 10200.00 0.00 0.10323 20000.00 10200.00 0.00 0.10332 19600.00 10400.00 0.00 0.10454 19800.00 10400.00 0.00 0.10518 20000.00 10400.00 0.00 0.10523 19600.00 10600.00 0.00 0.10660 19800.00 10600.00 0.00 0.10720 20000.00 10600.00 0.00 0.10721 19600.00 10800.00 0.00 0.10861 19800.00 10800.00 0.00 0.10917 19600.00 11000.00 0.00 0.11058 19800.00 11000.00 0.00 0.11110 19600.00 11200.00 0.00 0.11255 19800.00 11200.00 0.00 0.11302 19600.00 11400.00 0.00 0.11444 19800.00 11400.00 0.00 0.11486 19600.00 11600.00 0.00 0.11621 19800.00 11600.00 0.00 0.11658 19600.00 11800.00 0.00 0.11794 19800.00 11800.00 0.00 0.11825 19400.00 12000.00 0.00 0.11831 19600.00 12000.00 0.00 0.11964 19800.00 12000.00 0.00 0.11990 19400.00 12200.00 0.00 0.12014 19600.00 12200.00 0.00 0.12144 19800.00 12200.00 0.00 0.12166 19400.00 12400.00 0.00 0.12225 19600.00 12400.00 0.00 0.12353 19800.00 12400.00 0.00 0.12371 19400.00 12600.00 0.00 0.12463 19600.00 12600.00 0.00 0.12587 19800.00 12600.00 0.00 0.12603 19400.00 12800.00 0.00 0.12710 19600.00 12800.00 0.00 0.12833 19800.00 12800.00 0.00 0.12846 19400.00 13000.00 0.00 0.12961 19600.00 13000.00 0.00 0.13084 19800.00 13000.00 0.00 0.13096 19400.00 13200.00 0.00 0.13210 19600.00 13200.00 0.00 0.13333 19800.00 13200.00 0.00 0.13344 19400.00 13400.00 0.00 0.13459 19600.00 13400.00 0.00 0.13581 19800.00 13400.00 0.00 0.13588 19400.00 13600.00 0.00 0.13727 19600.00 13600.00 0.00 0.13847 19800.00 13600.00 0.00 0.13849 19400.00 13800.00 0.00 0.14029 19600.00 13800.00 0.00 0.14145 19400.00 14000.00 0.00 0.14357 19600.00 14000.00 0.00 0.14468 19400.00 14200.00 0.00 0.14705 19600.00 14200.00 0.00 0.14810 19200.00 14400.00 0.00 0.14818 19400.00 14400.00 0.00 0.15063 19600.00 14400.00 0.00 0.15162 19200.00 14600.00 0.00 0.15174 19400.00 14600.00 0.00 0.15415 19600.00 14600.00 0.00 0.15507 19200.00 14800.00 0.00 0.15528 19400.00 14800.00 0.00 0.15764 19600.00 14800.00 0.00 0.15849 19200.00 15000.00 0.00 0.15894 19400.00 15000.00 0.00 0.16126 19600.00 15000.00 0.00 0.16206 19200.00 15200.00 0.00 0.16265 19400.00 15200.00 0.00 0.16493 19600.00 15200.00 0.00 0.16567 19200.00 15400.00 0.00 0.16631 19400.00 15400.00 0.00 0.16855 19600.00 15400.00 0.00 0.16924 19200.00 15600.00 0.00 0.16995 19400.00 15600.00 0.00 0.17217 19600.00 15600.00 0.00 0.17280 19200.00 15800.00 0.00 0.17354 19400.00 15800.00 0.00 0.17573 19600.00 15800.00 0.00 0.17628 19200.00 16000.00 0.00 0.17713 19400.00 16000.00 0.00 0.17926 19600.00 16000.00 0.00 0.17973 19200.00 16200.00 0.00 0.18097 19400.00 16200.00 0.00 0.18304 19600.00 16200.00 0.00 0.18340 19200.00 16400.00 0.00 0.18511 19400.00 16400.00 0.00 0.18711 19600.00 16400.00 0.00 0.18735 19200.00 16600.00 0.00 0.18941 19400.00 16600.00 0.00 0.19132 19600.00 16600.00 0.00 0.19143 19200.00 16800.00 0.00 0.19384 19400.00 16800.00 0.00 0.19564 19200.00 17000.00 0.00 0.19833 19400.00 17000.00 0.00 0.20003 19200.00 17200.00 0.00 0.20280 19400.00 17200.00 0.00 0.20437 19200.00 17400.00 0.00 0.20745 19400.00 17400.00 0.00 0.20885 19000.00 17600.00 0.00 0.20912 19200.00 17600.00 0.00 0.21241 19400.00 17600.00 0.00 0.21361 19000.00 17800.00 0.00 0.21434 19200.00 17800.00 0.00 0.21747 19400.00 17800.00 0.00 0.21844 19000.00 18000.00 0.00 0.21959 19200.00 18000.00 0.00 0.22252 19400.00 18000.00 0.00 0.22319 19000.00 18200.00 0.00 0.22483 19200.00 18200.00 0.00 0.22748 19400.00 18200.00 0.00 0.22778 19000.00 18400.00 0.00 0.22969 19200.00 18400.00 0.00 0.23200 19000.00 18600.00 0.00 0.23403 19200.00 18600.00 0.00 0.23592 19000.00 18800.00 0.00 0.23787 19200.00 18800.00 0.00 0.23919 19000.00 19000.00 0.00 0.24050 19200.00 19000.00 0.00 0.24106 19000.00 19200.00 10.00 0.24115 19000.00 19200.00 15.00 0.24125 19000.00 19200.00 20.00 0.24133 19000.00 19200.00 25.00 0.24141 19000.00 19200.00 30.00 0.24148 19000.00 19200.00 35.00 0.24153 19000.00 19200.00 40.00 0.24156 19000.00 19200.00 45.00 0.24158 19000.00 19200.00 50.00 0.24158 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19043.68 19185.20 46.05 0.24165 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/242.ctf NX, NY, NZ: 360 360 1 MODE : real