CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/243.mrc Output diagnostic file name Micrographs/243.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/243.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2264.000 7900.000 Mean, RMS : 4433.004 228.0812 TITLE 1: emi2mrc: 243.emi Sun Jan 19 22:28:10 2014 TITLE 2: kV 300, Mag 78000x, Def 22480, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -490.389, Y 529.427, Z -23.067, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00798 8200.00 8000.00 0.00 -0.00759 8400.00 8000.00 0.00 -0.00714 8600.00 8000.00 0.00 -0.00670 8800.00 8000.00 0.00 -0.00632 9000.00 8000.00 0.00 -0.00599 9200.00 8000.00 0.00 -0.00568 9400.00 8000.00 0.00 -0.00538 9600.00 8000.00 0.00 -0.00510 9800.00 8000.00 0.00 -0.00483 10000.00 8000.00 0.00 -0.00459 10200.00 8000.00 0.00 -0.00434 10400.00 8000.00 0.00 -0.00405 10600.00 8000.00 0.00 -0.00367 10800.00 8000.00 0.00 -0.00320 11000.00 8000.00 0.00 -0.00264 11200.00 8000.00 0.00 -0.00200 11400.00 8000.00 0.00 -0.00127 11600.00 8000.00 0.00 -0.00040 11800.00 8000.00 0.00 0.00063 12000.00 8000.00 0.00 0.00184 12200.00 8000.00 0.00 0.00316 12400.00 8000.00 0.00 0.00456 12600.00 8000.00 0.00 0.00596 12800.00 8000.00 0.00 0.00737 13000.00 8000.00 0.00 0.00880 13200.00 8000.00 0.00 0.01027 13400.00 8000.00 0.00 0.01180 13600.00 8000.00 0.00 0.01340 13800.00 8000.00 0.00 0.01509 14000.00 8000.00 0.00 0.01686 14200.00 8000.00 0.00 0.01873 14400.00 8000.00 0.00 0.02069 14600.00 8000.00 0.00 0.02275 14800.00 8000.00 0.00 0.02490 15000.00 8000.00 0.00 0.02715 15200.00 8000.00 0.00 0.02954 15400.00 8000.00 0.00 0.03206 15600.00 8000.00 0.00 0.03471 15800.00 8000.00 0.00 0.03743 16000.00 8000.00 0.00 0.04013 16200.00 8000.00 0.00 0.04277 16400.00 8000.00 0.00 0.04538 16600.00 8000.00 0.00 0.04799 16800.00 8000.00 0.00 0.05068 17000.00 8000.00 0.00 0.05353 17200.00 8000.00 0.00 0.05653 17400.00 8000.00 0.00 0.05965 17600.00 8000.00 0.00 0.06288 17800.00 8000.00 0.00 0.06621 18000.00 8000.00 0.00 0.06966 18200.00 8000.00 0.00 0.07326 18400.00 8000.00 0.00 0.07698 18600.00 8000.00 0.00 0.08068 18800.00 8000.00 0.00 0.08418 19000.00 8000.00 0.00 0.08723 19200.00 8000.00 0.00 0.08958 19400.00 8000.00 0.00 0.09115 19600.00 8000.00 0.00 0.09199 19800.00 8000.00 0.00 0.09232 20000.00 8000.00 0.00 0.09241 20200.00 8000.00 0.00 0.09247 20400.00 8000.00 0.00 0.09253 20600.00 8000.00 0.00 0.09256 19600.00 8200.00 0.00 0.09305 19800.00 8200.00 0.00 0.09339 20000.00 8200.00 0.00 0.09349 20200.00 8200.00 0.00 0.09355 20400.00 8200.00 0.00 0.09360 20600.00 8200.00 0.00 0.09361 19600.00 8400.00 0.00 0.09404 19800.00 8400.00 0.00 0.09438 20000.00 8400.00 0.00 0.09449 20200.00 8400.00 0.00 0.09455 20400.00 8400.00 0.00 0.09460 19600.00 8600.00 0.00 0.09516 19800.00 8600.00 0.00 0.09553 20000.00 8600.00 0.00 0.09564 20200.00 8600.00 0.00 0.09570 20400.00 8600.00 0.00 0.09572 19600.00 8800.00 0.00 0.09647 19800.00 8800.00 0.00 0.09684 20000.00 8800.00 0.00 0.09695 20200.00 8800.00 0.00 0.09699 19600.00 9000.00 0.00 0.09786 19800.00 9000.00 0.00 0.09823 20000.00 9000.00 0.00 0.09833 20200.00 9000.00 0.00 0.09835 19400.00 9200.00 0.00 0.09835 19600.00 9200.00 0.00 0.09928 19800.00 9200.00 0.00 0.09965 20000.00 9200.00 0.00 0.09973 19600.00 9400.00 0.00 0.10065 19800.00 9400.00 0.00 0.10101 20000.00 9400.00 0.00 0.10108 19600.00 9600.00 0.00 0.10197 19800.00 9600.00 0.00 0.10234 20000.00 9600.00 0.00 0.10240 19400.00 9800.00 0.00 0.10248 19600.00 9800.00 0.00 0.10342 19800.00 9800.00 0.00 0.10378 20000.00 9800.00 0.00 0.10383 19400.00 10000.00 0.00 0.10412 19600.00 10000.00 0.00 0.10508 19800.00 10000.00 0.00 0.10543 20000.00 10000.00 0.00 0.10545 19400.00 10200.00 0.00 0.10588 19600.00 10200.00 0.00 0.10683 19800.00 10200.00 0.00 0.10716 19400.00 10400.00 0.00 0.10765 19600.00 10400.00 0.00 0.10859 19800.00 10400.00 0.00 0.10889 19400.00 10600.00 0.00 0.10936 19600.00 10600.00 0.00 0.11028 19800.00 10600.00 0.00 0.11055 19400.00 10800.00 0.00 0.11098 19600.00 10800.00 0.00 0.11188 19800.00 10800.00 0.00 0.11213 19400.00 11000.00 0.00 0.11267 19600.00 11000.00 0.00 0.11356 19800.00 11000.00 0.00 0.11378 19400.00 11200.00 0.00 0.11460 19600.00 11200.00 0.00 0.11547 19800.00 11200.00 0.00 0.11566 19400.00 11400.00 0.00 0.11670 19600.00 11400.00 0.00 0.11756 19800.00 11400.00 0.00 0.11772 19400.00 11600.00 0.00 0.11885 19600.00 11600.00 0.00 0.11970 19800.00 11600.00 0.00 0.11983 19400.00 11800.00 0.00 0.12095 19600.00 11800.00 0.00 0.12178 19800.00 11800.00 0.00 0.12189 19400.00 12000.00 0.00 0.12289 19600.00 12000.00 0.00 0.12371 19800.00 12000.00 0.00 0.12380 19400.00 12200.00 0.00 0.12476 19600.00 12200.00 0.00 0.12557 19800.00 12200.00 0.00 0.12563 19400.00 12400.00 0.00 0.12683 19600.00 12400.00 0.00 0.12763 19800.00 12400.00 0.00 0.12766 19400.00 12600.00 0.00 0.12920 19600.00 12600.00 0.00 0.12998 19200.00 12800.00 0.00 0.13005 19400.00 12800.00 0.00 0.13180 19600.00 12800.00 0.00 0.13256 19200.00 13000.00 0.00 0.13281 19400.00 13000.00 0.00 0.13457 19600.00 13000.00 0.00 0.13531 19200.00 13200.00 0.00 0.13562 19400.00 13200.00 0.00 0.13738 19600.00 13200.00 0.00 0.13811 19200.00 13400.00 0.00 0.13839 19400.00 13400.00 0.00 0.14015 19600.00 13400.00 0.00 0.14086 19200.00 13600.00 0.00 0.14128 19400.00 13600.00 0.00 0.14304 19600.00 13600.00 0.00 0.14370 19200.00 13800.00 0.00 0.14443 19400.00 13800.00 0.00 0.14616 19600.00 13800.00 0.00 0.14678 19200.00 14000.00 0.00 0.14775 19400.00 14000.00 0.00 0.14947 19600.00 14000.00 0.00 0.15003 19200.00 14200.00 0.00 0.15122 19400.00 14200.00 0.00 0.15291 19600.00 14200.00 0.00 0.15342 19200.00 14400.00 0.00 0.15478 19400.00 14400.00 0.00 0.15644 19600.00 14400.00 0.00 0.15689 19200.00 14600.00 0.00 0.15830 19400.00 14600.00 0.00 0.15992 19600.00 14600.00 0.00 0.16029 19200.00 14800.00 0.00 0.16188 19400.00 14800.00 0.00 0.16345 19600.00 14800.00 0.00 0.16374 19200.00 15000.00 0.00 0.16575 19400.00 15000.00 0.00 0.16725 19600.00 15000.00 0.00 0.16744 19200.00 15200.00 0.00 0.16983 19400.00 15200.00 0.00 0.17125 19600.00 15200.00 0.00 0.17134 19200.00 15400.00 0.00 0.17396 19400.00 15400.00 0.00 0.17530 19000.00 15600.00 0.00 0.17538 19200.00 15600.00 0.00 0.17812 19400.00 15600.00 0.00 0.17936 19000.00 15800.00 0.00 0.17947 19200.00 15800.00 0.00 0.18215 19400.00 15800.00 0.00 0.18331 19000.00 16000.00 0.00 0.18348 19200.00 16000.00 0.00 0.18610 19400.00 16000.00 0.00 0.18715 19000.00 16200.00 0.00 0.18772 19200.00 16200.00 0.00 0.19026 19400.00 16200.00 0.00 0.19120 19000.00 16400.00 0.00 0.19230 19200.00 16400.00 0.00 0.19475 19400.00 16400.00 0.00 0.19556 19000.00 16600.00 0.00 0.19704 19200.00 16600.00 0.00 0.19938 19400.00 16600.00 0.00 0.20004 19000.00 16800.00 0.00 0.20180 19200.00 16800.00 0.00 0.20401 19400.00 16800.00 0.00 0.20451 19000.00 17000.00 0.00 0.20636 19200.00 17000.00 0.00 0.20845 19400.00 17000.00 0.00 0.20878 19000.00 17200.00 0.00 0.21062 19200.00 17200.00 0.00 0.21258 19400.00 17200.00 0.00 0.21272 19000.00 17400.00 0.00 0.21493 19200.00 17400.00 0.00 0.21674 19000.00 17600.00 0.00 0.21976 19200.00 17600.00 0.00 0.22137 18800.00 17800.00 0.00 0.22187 19000.00 17800.00 0.00 0.22515 19200.00 17800.00 0.00 0.22651 18800.00 18000.00 0.00 0.22806 19000.00 18000.00 0.00 0.23106 19200.00 18000.00 0.00 0.23206 18800.00 18200.00 0.00 0.23454 19000.00 18200.00 0.00 0.23713 19200.00 18200.00 0.00 0.23764 18800.00 18400.00 0.00 0.24034 19000.00 18400.00 0.00 0.24240 18800.00 18600.00 0.00 0.24468 19000.00 18600.00 0.00 0.24618 18800.00 18800.00 0.00 0.24736 19000.00 18800.00 0.00 0.24822 18800.00 19000.00 15.00 0.24826 18800.00 19000.00 20.00 0.24838 18800.00 19000.00 25.00 0.24848 18800.00 19000.00 30.00 0.24858 18800.00 19000.00 35.00 0.24865 18800.00 19000.00 40.00 0.24871 18800.00 19000.00 45.00 0.24874 18800.00 19000.00 50.00 0.24876 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18837.53 19016.32 52.13 0.24882 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/243.ctf NX, NY, NZ: 360 360 1 MODE : real