CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/246.mrc Output diagnostic file name Micrographs/246.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/246.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2398.000 7780.000 Mean, RMS : 4432.452 222.6067 TITLE 1: emi2mrc: 246.emi Sun Jan 19 22:31:55 2014 TITLE 2: kV 300, Mag 78000x, Def 22480, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -493.194, Y 521.646, Z -23.067, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01019 8200.00 8000.00 0.00 -0.01001 8400.00 8000.00 0.00 -0.00972 8600.00 8000.00 0.00 -0.00935 8800.00 8000.00 0.00 -0.00897 9000.00 8000.00 0.00 -0.00856 9200.00 8000.00 0.00 -0.00810 9400.00 8000.00 0.00 -0.00754 9600.00 8000.00 0.00 -0.00687 9800.00 8000.00 0.00 -0.00609 10000.00 8000.00 0.00 -0.00525 10200.00 8000.00 0.00 -0.00439 10400.00 8000.00 0.00 -0.00353 10600.00 8000.00 0.00 -0.00262 10800.00 8000.00 0.00 -0.00163 11000.00 8000.00 0.00 -0.00051 11200.00 8000.00 0.00 0.00074 11400.00 8000.00 0.00 0.00209 11600.00 8000.00 0.00 0.00352 11800.00 8000.00 0.00 0.00499 12000.00 8000.00 0.00 0.00648 12200.00 8000.00 0.00 0.00799 12400.00 8000.00 0.00 0.00950 12600.00 8000.00 0.00 0.01098 12800.00 8000.00 0.00 0.01243 13000.00 8000.00 0.00 0.01389 13200.00 8000.00 0.00 0.01547 13400.00 8000.00 0.00 0.01727 13600.00 8000.00 0.00 0.01939 13800.00 8000.00 0.00 0.02186 14000.00 8000.00 0.00 0.02457 14200.00 8000.00 0.00 0.02738 14400.00 8000.00 0.00 0.03014 14600.00 8000.00 0.00 0.03279 14800.00 8000.00 0.00 0.03536 15000.00 8000.00 0.00 0.03797 15200.00 8000.00 0.00 0.04074 15400.00 8000.00 0.00 0.04368 15600.00 8000.00 0.00 0.04676 15800.00 8000.00 0.00 0.04990 16000.00 8000.00 0.00 0.05301 16200.00 8000.00 0.00 0.05610 16400.00 8000.00 0.00 0.05921 16600.00 8000.00 0.00 0.06235 16800.00 8000.00 0.00 0.06555 17000.00 8000.00 0.00 0.06886 17200.00 8000.00 0.00 0.07232 17400.00 8000.00 0.00 0.07602 17600.00 8000.00 0.00 0.08002 17800.00 8000.00 0.00 0.08422 18000.00 8000.00 0.00 0.08831 18200.00 8000.00 0.00 0.09193 18400.00 8000.00 0.00 0.09470 18600.00 8000.00 0.00 0.09648 18800.00 8000.00 0.00 0.09739 19000.00 8000.00 0.00 0.09775 19200.00 8000.00 0.00 0.09785 19400.00 8000.00 0.00 0.09792 19600.00 8000.00 0.00 0.09801 19800.00 8000.00 0.00 0.09803 18800.00 8200.00 0.00 0.09876 19000.00 8200.00 0.00 0.09911 19200.00 8200.00 0.00 0.09920 19400.00 8200.00 0.00 0.09927 19600.00 8200.00 0.00 0.09936 19800.00 8200.00 0.00 0.09936 18600.00 8400.00 0.00 0.09937 18800.00 8400.00 0.00 0.10024 19000.00 8400.00 0.00 0.10056 19200.00 8400.00 0.00 0.10064 19400.00 8400.00 0.00 0.10070 19600.00 8400.00 0.00 0.10078 18600.00 8600.00 0.00 0.10110 18800.00 8600.00 0.00 0.10193 19000.00 8600.00 0.00 0.10223 19200.00 8600.00 0.00 0.10229 19400.00 8600.00 0.00 0.10233 19600.00 8600.00 0.00 0.10239 18600.00 8800.00 0.00 0.10298 18800.00 8800.00 0.00 0.10377 19000.00 8800.00 0.00 0.10402 19200.00 8800.00 0.00 0.10406 19400.00 8800.00 0.00 0.10408 19600.00 8800.00 0.00 0.10412 18600.00 9000.00 0.00 0.10480 18800.00 9000.00 0.00 0.10553 19000.00 9000.00 0.00 0.10575 19200.00 9000.00 0.00 0.10576 19400.00 9000.00 0.00 0.10577 19600.00 9000.00 0.00 0.10578 18600.00 9200.00 0.00 0.10644 18800.00 9200.00 0.00 0.10713 19000.00 9200.00 0.00 0.10732 18600.00 9400.00 0.00 0.10786 18800.00 9400.00 0.00 0.10853 19000.00 9400.00 0.00 0.10870 18600.00 9600.00 0.00 0.10915 18800.00 9600.00 0.00 0.10979 19000.00 9600.00 0.00 0.10996 18600.00 9800.00 0.00 0.11055 18800.00 9800.00 0.00 0.11119 19000.00 9800.00 0.00 0.11135 18600.00 10000.00 0.00 0.11221 18800.00 10000.00 0.00 0.11284 19000.00 10000.00 0.00 0.11300 18600.00 10200.00 0.00 0.11408 18800.00 10200.00 0.00 0.11471 19000.00 10200.00 0.00 0.11487 18600.00 10400.00 0.00 0.11608 18800.00 10400.00 0.00 0.11671 19000.00 10400.00 0.00 0.11687 18600.00 10600.00 0.00 0.11814 18800.00 10600.00 0.00 0.11876 19000.00 10600.00 0.00 0.11891 18600.00 10800.00 0.00 0.12017 18800.00 10800.00 0.00 0.12077 19000.00 10800.00 0.00 0.12090 18600.00 11000.00 0.00 0.12225 18800.00 11000.00 0.00 0.12283 19000.00 11000.00 0.00 0.12293 18400.00 11200.00 0.00 0.12313 18600.00 11200.00 0.00 0.12450 18800.00 11200.00 0.00 0.12505 19000.00 11200.00 0.00 0.12511 18400.00 11400.00 0.00 0.12547 18600.00 11400.00 0.00 0.12682 18800.00 11400.00 0.00 0.12733 19000.00 11400.00 0.00 0.12736 18400.00 11600.00 0.00 0.12778 18600.00 11600.00 0.00 0.12910 18800.00 11600.00 0.00 0.12958 18400.00 11800.00 0.00 0.13006 18600.00 11800.00 0.00 0.13136 18800.00 11800.00 0.00 0.13182 18400.00 12000.00 0.00 0.13230 18600.00 12000.00 0.00 0.13358 18800.00 12000.00 0.00 0.13401 18400.00 12200.00 0.00 0.13460 18600.00 12200.00 0.00 0.13587 18800.00 12200.00 0.00 0.13628 18400.00 12400.00 0.00 0.13721 18600.00 12400.00 0.00 0.13848 18800.00 12400.00 0.00 0.13889 18400.00 12600.00 0.00 0.14014 18600.00 12600.00 0.00 0.14141 18800.00 12600.00 0.00 0.14182 18400.00 12800.00 0.00 0.14320 18600.00 12800.00 0.00 0.14449 18800.00 12800.00 0.00 0.14491 18400.00 13000.00 0.00 0.14633 18600.00 13000.00 0.00 0.14763 18800.00 13000.00 0.00 0.14804 18400.00 13200.00 0.00 0.14941 18600.00 13200.00 0.00 0.15072 18800.00 13200.00 0.00 0.15112 18400.00 13400.00 0.00 0.15242 18600.00 13400.00 0.00 0.15375 18800.00 13400.00 0.00 0.15414 18400.00 13600.00 0.00 0.15562 18600.00 13600.00 0.00 0.15697 18800.00 13600.00 0.00 0.15733 18400.00 13800.00 0.00 0.15916 18600.00 13800.00 0.00 0.16051 18800.00 13800.00 0.00 0.16084 18400.00 14000.00 0.00 0.16289 18600.00 14000.00 0.00 0.16422 18800.00 14000.00 0.00 0.16447 18400.00 14200.00 0.00 0.16668 18600.00 14200.00 0.00 0.16796 18800.00 14200.00 0.00 0.16812 18400.00 14400.00 0.00 0.17049 18600.00 14400.00 0.00 0.17169 18800.00 14400.00 0.00 0.17171 18400.00 14600.00 0.00 0.17419 18600.00 14600.00 0.00 0.17528 18200.00 14800.00 0.00 0.17540 18400.00 14800.00 0.00 0.17794 18600.00 14800.00 0.00 0.17890 18200.00 15000.00 0.00 0.17950 18400.00 15000.00 0.00 0.18195 18600.00 15000.00 0.00 0.18276 18200.00 15200.00 0.00 0.18376 18400.00 15200.00 0.00 0.18610 18600.00 15200.00 0.00 0.18676 18200.00 15400.00 0.00 0.18792 18400.00 15400.00 0.00 0.19017 18600.00 15400.00 0.00 0.19068 18200.00 15600.00 0.00 0.19194 18400.00 15600.00 0.00 0.19409 18600.00 15600.00 0.00 0.19444 18200.00 15800.00 0.00 0.19574 18400.00 15800.00 0.00 0.19778 18600.00 15800.00 0.00 0.19797 18200.00 16000.00 0.00 0.19941 18400.00 16000.00 0.00 0.20133 18600.00 16000.00 0.00 0.20136 18200.00 16200.00 0.00 0.20333 18400.00 16200.00 0.00 0.20511 18200.00 16400.00 0.00 0.20755 18400.00 16400.00 0.00 0.20916 18200.00 16600.00 0.00 0.21178 18400.00 16600.00 0.00 0.21320 18200.00 16800.00 0.00 0.21591 18400.00 16800.00 0.00 0.21709 18200.00 17000.00 0.00 0.21982 18400.00 17000.00 0.00 0.22074 18200.00 17200.00 0.00 0.22349 18400.00 17200.00 0.00 0.22411 18000.00 17400.00 0.00 0.22462 18200.00 17400.00 0.00 0.22729 18400.00 17400.00 0.00 0.22752 18000.00 17600.00 0.00 0.22901 18200.00 17600.00 0.00 0.23127 18000.00 17800.00 0.00 0.23295 18200.00 17800.00 0.00 0.23465 18000.00 18000.00 0.00 0.23552 18200.00 18000.00 0.00 0.23653 18000.00 18200.00 20.00 0.23657 18000.00 18200.00 25.00 0.23666 18000.00 18200.00 30.00 0.23675 18000.00 18200.00 35.00 0.23683 18000.00 18200.00 40.00 0.23690 18000.00 18200.00 45.00 0.23694 18000.00 18200.00 50.00 0.23698 18000.00 18200.00 55.00 0.23699 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18058.32 18220.96 52.41 0.23712 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/246.ctf NX, NY, NZ: 360 360 1 MODE : real