CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/247.mrc Output diagnostic file name Micrographs/247.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/247.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2316.000 8388.000 Mean, RMS : 4432.663 218.2319 TITLE 1: emi2mrc: 247.emi Sun Jan 19 22:32:34 2014 TITLE 2: kV 300, Mag 78000x, Def 22480, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -491.675, Y 524.227, Z -23.067, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.02474 8200.00 8000.00 0.00 -0.02450 8400.00 8000.00 0.00 -0.02411 8600.00 8000.00 0.00 -0.02369 8800.00 8000.00 0.00 -0.02327 9000.00 8000.00 0.00 -0.02274 9200.00 8000.00 0.00 -0.02203 9400.00 8000.00 0.00 -0.02119 9600.00 8000.00 0.00 -0.02028 9800.00 8000.00 0.00 -0.01939 10000.00 8000.00 0.00 -0.01859 10200.00 8000.00 0.00 -0.01779 10400.00 8000.00 0.00 -0.01688 10600.00 8000.00 0.00 -0.01582 10800.00 8000.00 0.00 -0.01465 11000.00 8000.00 0.00 -0.01350 11200.00 8000.00 0.00 -0.01248 11400.00 8000.00 0.00 -0.01162 11600.00 8000.00 0.00 -0.01085 11800.00 8000.00 0.00 -0.01005 12000.00 8000.00 0.00 -0.00915 12200.00 8000.00 0.00 -0.00809 12400.00 8000.00 0.00 -0.00687 12600.00 8000.00 0.00 -0.00548 12800.00 8000.00 0.00 -0.00393 13000.00 8000.00 0.00 -0.00221 13200.00 8000.00 0.00 -0.00040 13400.00 8000.00 0.00 0.00142 13600.00 8000.00 0.00 0.00319 13800.00 8000.00 0.00 0.00491 14000.00 8000.00 0.00 0.00669 14200.00 8000.00 0.00 0.00862 14400.00 8000.00 0.00 0.01080 14600.00 8000.00 0.00 0.01321 14800.00 8000.00 0.00 0.01581 15000.00 8000.00 0.00 0.01853 15200.00 8000.00 0.00 0.02134 15400.00 8000.00 0.00 0.02423 15600.00 8000.00 0.00 0.02717 15800.00 8000.00 0.00 0.03010 16000.00 8000.00 0.00 0.03293 16200.00 8000.00 0.00 0.03568 16400.00 8000.00 0.00 0.03843 16600.00 8000.00 0.00 0.04128 16800.00 8000.00 0.00 0.04432 17000.00 8000.00 0.00 0.04754 17200.00 8000.00 0.00 0.05080 17400.00 8000.00 0.00 0.05401 17600.00 8000.00 0.00 0.05719 17800.00 8000.00 0.00 0.06046 18000.00 8000.00 0.00 0.06396 18200.00 8000.00 0.00 0.06778 18400.00 8000.00 0.00 0.07178 18600.00 8000.00 0.00 0.07567 18800.00 8000.00 0.00 0.07912 19000.00 8000.00 0.00 0.08187 19200.00 8000.00 0.00 0.08381 19400.00 8000.00 0.00 0.08501 19600.00 8000.00 0.00 0.08564 19800.00 8000.00 0.00 0.08587 19400.00 8200.00 0.00 0.08607 19600.00 8200.00 0.00 0.08668 19800.00 8200.00 0.00 0.08688 19400.00 8400.00 0.00 0.08716 19600.00 8400.00 0.00 0.08775 19800.00 8400.00 0.00 0.08791 19400.00 8600.00 0.00 0.08844 19600.00 8600.00 0.00 0.08899 19800.00 8600.00 0.00 0.08912 19400.00 8800.00 0.00 0.08989 19600.00 8800.00 0.00 0.09041 19800.00 8800.00 0.00 0.09049 19400.00 9000.00 0.00 0.09141 19600.00 9000.00 0.00 0.09188 19800.00 9000.00 0.00 0.09191 19400.00 9200.00 0.00 0.09292 19600.00 9200.00 0.00 0.09334 19400.00 9400.00 0.00 0.09436 19600.00 9400.00 0.00 0.09474 19200.00 9600.00 0.00 0.09477 19400.00 9600.00 0.00 0.09574 19600.00 9600.00 0.00 0.09607 19200.00 9800.00 0.00 0.09627 19400.00 9800.00 0.00 0.09719 19600.00 9800.00 0.00 0.09748 19200.00 10000.00 0.00 0.09791 19400.00 10000.00 0.00 0.09880 19600.00 10000.00 0.00 0.09906 19200.00 10200.00 0.00 0.09961 19400.00 10200.00 0.00 0.10047 19600.00 10200.00 0.00 0.10069 19200.00 10400.00 0.00 0.10130 19400.00 10400.00 0.00 0.10212 19600.00 10400.00 0.00 0.10231 19200.00 10600.00 0.00 0.10293 19400.00 10600.00 0.00 0.10372 19600.00 10600.00 0.00 0.10386 19200.00 10800.00 0.00 0.10448 19400.00 10800.00 0.00 0.10523 19600.00 10800.00 0.00 0.10534 19200.00 11000.00 0.00 0.10608 19400.00 11000.00 0.00 0.10680 19600.00 11000.00 0.00 0.10688 19200.00 11200.00 0.00 0.10788 19400.00 11200.00 0.00 0.10857 19600.00 11200.00 0.00 0.10863 19200.00 11400.00 0.00 0.10985 19400.00 11400.00 0.00 0.11053 19600.00 11400.00 0.00 0.11057 19200.00 11600.00 0.00 0.11194 19400.00 11600.00 0.00 0.11260 19600.00 11600.00 0.00 0.11263 19200.00 11800.00 0.00 0.11412 19400.00 11800.00 0.00 0.11478 19600.00 11800.00 0.00 0.11481 19200.00 12000.00 0.00 0.11634 19400.00 12000.00 0.00 0.11701 19600.00 12000.00 0.00 0.11704 19000.00 12200.00 0.00 0.11711 19200.00 12200.00 0.00 0.11867 19400.00 12200.00 0.00 0.11935 19600.00 12200.00 0.00 0.11938 19000.00 12400.00 0.00 0.11968 19200.00 12400.00 0.00 0.12125 19400.00 12400.00 0.00 0.12192 19600.00 12400.00 0.00 0.12193 19000.00 12600.00 0.00 0.12245 19200.00 12600.00 0.00 0.12401 19400.00 12600.00 0.00 0.12467 19000.00 12800.00 0.00 0.12522 19200.00 12800.00 0.00 0.12676 19400.00 12800.00 0.00 0.12738 19000.00 13000.00 0.00 0.12790 19200.00 13000.00 0.00 0.12942 19400.00 13000.00 0.00 0.13000 19000.00 13200.00 0.00 0.13045 19200.00 13200.00 0.00 0.13194 19400.00 13200.00 0.00 0.13247 19000.00 13400.00 0.00 0.13292 19200.00 13400.00 0.00 0.13438 19400.00 13400.00 0.00 0.13487 19000.00 13600.00 0.00 0.13558 19200.00 13600.00 0.00 0.13701 19400.00 13600.00 0.00 0.13745 19000.00 13800.00 0.00 0.13858 19200.00 13800.00 0.00 0.13996 19400.00 13800.00 0.00 0.14035 19000.00 14000.00 0.00 0.14176 19200.00 14000.00 0.00 0.14311 19400.00 14000.00 0.00 0.14344 19000.00 14200.00 0.00 0.14499 19200.00 14200.00 0.00 0.14630 19400.00 14200.00 0.00 0.14657 19000.00 14400.00 0.00 0.14814 19200.00 14400.00 0.00 0.14940 19400.00 14400.00 0.00 0.14960 19000.00 14600.00 0.00 0.15107 19200.00 14600.00 0.00 0.15229 19400.00 14600.00 0.00 0.15241 19000.00 14800.00 0.00 0.15394 19200.00 14800.00 0.00 0.15510 19400.00 14800.00 0.00 0.15515 19000.00 15000.00 0.00 0.15702 19200.00 15000.00 0.00 0.15812 19000.00 15200.00 0.00 0.16032 19200.00 15200.00 0.00 0.16135 18800.00 15400.00 0.00 0.16138 19000.00 15400.00 0.00 0.16372 19200.00 15400.00 0.00 0.16468 18800.00 15600.00 0.00 0.16489 19000.00 15600.00 0.00 0.16720 19200.00 15600.00 0.00 0.16808 18800.00 15800.00 0.00 0.16837 19000.00 15800.00 0.00 0.17063 19200.00 15800.00 0.00 0.17143 18800.00 16000.00 0.00 0.17181 19000.00 16000.00 0.00 0.17400 19200.00 16000.00 0.00 0.17469 18800.00 16200.00 0.00 0.17543 19000.00 16200.00 0.00 0.17752 19200.00 16200.00 0.00 0.17805 18800.00 16400.00 0.00 0.17923 19000.00 16400.00 0.00 0.18117 19200.00 16400.00 0.00 0.18150 18800.00 16600.00 0.00 0.18293 19000.00 16600.00 0.00 0.18468 19200.00 16600.00 0.00 0.18477 18800.00 16800.00 0.00 0.18637 19000.00 16800.00 0.00 0.18790 18800.00 17000.00 0.00 0.18946 19000.00 17000.00 0.00 0.19077 18800.00 17200.00 0.00 0.19224 19000.00 17200.00 0.00 0.19330 18800.00 17400.00 0.00 0.19512 19000.00 17400.00 0.00 0.19590 18600.00 17600.00 0.00 0.19605 18800.00 17600.00 0.00 0.19839 19000.00 17600.00 0.00 0.19882 18600.00 17800.00 0.00 0.19985 18800.00 17800.00 0.00 0.20180 19000.00 17800.00 0.00 0.20181 18600.00 18000.00 0.00 0.20349 18800.00 18000.00 0.00 0.20496 18600.00 18200.00 0.00 0.20654 18800.00 18200.00 0.00 0.20745 18600.00 18400.00 0.00 0.20835 18800.00 18400.00 0.00 0.20870 18600.00 18600.00 0.00 0.20882 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18700.82 18534.71 11.93 0.20904 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/247.ctf NX, NY, NZ: 360 360 1 MODE : real