CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/254.mrc Output diagnostic file name Micrographs/254.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/254.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2270.000 9570.000 Mean, RMS : 4428.189 244.0510 TITLE 1: emi2mrc: 254.emi Sun Jan 19 22:43:26 2014 TITLE 2: kV 300, Mag 78000x, Def 21330, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -475.087, Y 544.605, Z -22.843, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 117 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.02044 8200.00 8000.00 0.00 -0.02027 8400.00 8000.00 0.00 -0.01987 8600.00 8000.00 0.00 -0.01940 8800.00 8000.00 0.00 -0.01899 9000.00 8000.00 0.00 -0.01867 9200.00 8000.00 0.00 -0.01840 9400.00 8000.00 0.00 -0.01813 9600.00 8000.00 0.00 -0.01782 9800.00 8000.00 0.00 -0.01742 10000.00 8000.00 0.00 -0.01701 10200.00 8000.00 0.00 -0.01661 10400.00 8000.00 0.00 -0.01621 10600.00 8000.00 0.00 -0.01578 10800.00 8000.00 0.00 -0.01528 11000.00 8000.00 0.00 -0.01465 11200.00 8000.00 0.00 -0.01388 11400.00 8000.00 0.00 -0.01299 11600.00 8000.00 0.00 -0.01198 11800.00 8000.00 0.00 -0.01090 12000.00 8000.00 0.00 -0.00977 12200.00 8000.00 0.00 -0.00864 12400.00 8000.00 0.00 -0.00758 12600.00 8000.00 0.00 -0.00661 12800.00 8000.00 0.00 -0.00569 13000.00 8000.00 0.00 -0.00478 13200.00 8000.00 0.00 -0.00380 13400.00 8000.00 0.00 -0.00272 13600.00 8000.00 0.00 -0.00153 13800.00 8000.00 0.00 -0.00026 14000.00 8000.00 0.00 0.00107 14200.00 8000.00 0.00 0.00247 14400.00 8000.00 0.00 0.00401 14600.00 8000.00 0.00 0.00573 14800.00 8000.00 0.00 0.00766 15000.00 8000.00 0.00 0.00976 15200.00 8000.00 0.00 0.01198 15400.00 8000.00 0.00 0.01426 15600.00 8000.00 0.00 0.01660 15800.00 8000.00 0.00 0.01902 16000.00 8000.00 0.00 0.02153 16200.00 8000.00 0.00 0.02414 16400.00 8000.00 0.00 0.02683 16600.00 8000.00 0.00 0.02953 16800.00 8000.00 0.00 0.03226 17000.00 8000.00 0.00 0.03505 17200.00 8000.00 0.00 0.03794 17400.00 8000.00 0.00 0.04096 17600.00 8000.00 0.00 0.04412 17800.00 8000.00 0.00 0.04737 18000.00 8000.00 0.00 0.05066 18200.00 8000.00 0.00 0.05402 18400.00 8000.00 0.00 0.05749 18600.00 8000.00 0.00 0.06107 18800.00 8000.00 0.00 0.06470 19000.00 8000.00 0.00 0.06813 19200.00 8000.00 0.00 0.07100 19400.00 8000.00 0.00 0.07302 19600.00 8000.00 0.00 0.07406 19800.00 8000.00 0.00 0.07423 19600.00 8200.00 0.00 0.07510 19800.00 8200.00 0.00 0.07529 19600.00 8400.00 0.00 0.07621 19800.00 8400.00 0.00 0.07641 19400.00 8600.00 0.00 0.07641 19600.00 8600.00 0.00 0.07747 19800.00 8600.00 0.00 0.07770 19400.00 8800.00 0.00 0.07777 19600.00 8800.00 0.00 0.07885 19800.00 8800.00 0.00 0.07910 19400.00 9000.00 0.00 0.07914 19600.00 9000.00 0.00 0.08025 19800.00 9000.00 0.00 0.08051 19400.00 9200.00 0.00 0.08054 19600.00 9200.00 0.00 0.08166 19800.00 9200.00 0.00 0.08193 19400.00 9400.00 0.00 0.08196 19600.00 9400.00 0.00 0.08310 19800.00 9400.00 0.00 0.08336 19400.00 9600.00 0.00 0.08347 19600.00 9600.00 0.00 0.08461 19800.00 9600.00 0.00 0.08487 19400.00 9800.00 0.00 0.08520 19600.00 9800.00 0.00 0.08634 19800.00 9800.00 0.00 0.08660 19400.00 10000.00 0.00 0.08719 19600.00 10000.00 0.00 0.08832 19800.00 10000.00 0.00 0.08857 19400.00 10200.00 0.00 0.08930 19600.00 10200.00 0.00 0.09042 19800.00 10200.00 0.00 0.09066 19400.00 10400.00 0.00 0.09141 19600.00 10400.00 0.00 0.09252 19800.00 10400.00 0.00 0.09274 19400.00 10600.00 0.00 0.09344 19600.00 10600.00 0.00 0.09455 19800.00 10600.00 0.00 0.09475 19400.00 10800.00 0.00 0.09537 19600.00 10800.00 0.00 0.09647 19800.00 10800.00 0.00 0.09667 19400.00 11000.00 0.00 0.09732 19600.00 11000.00 0.00 0.09843 19800.00 11000.00 0.00 0.09863 19400.00 11200.00 0.00 0.09943 19600.00 11200.00 0.00 0.10054 19800.00 11200.00 0.00 0.10074 19400.00 11400.00 0.00 0.10164 19600.00 11400.00 0.00 0.10274 19800.00 11400.00 0.00 0.10293 19400.00 11600.00 0.00 0.10384 19600.00 11600.00 0.00 0.10493 19800.00 11600.00 0.00 0.10510 19400.00 11800.00 0.00 0.10598 19600.00 11800.00 0.00 0.10705 19800.00 11800.00 0.00 0.10720 19400.00 12000.00 0.00 0.10799 19600.00 12000.00 0.00 0.10906 19800.00 12000.00 0.00 0.10919 19400.00 12200.00 0.00 0.10997 19600.00 12200.00 0.00 0.11105 19800.00 12200.00 0.00 0.11117 19400.00 12400.00 0.00 0.11217 19600.00 12400.00 0.00 0.11327 19800.00 12400.00 0.00 0.11339 19400.00 12600.00 0.00 0.11465 19600.00 12600.00 0.00 0.11577 19800.00 12600.00 0.00 0.11590 19400.00 12800.00 0.00 0.11733 19600.00 12800.00 0.00 0.11848 19800.00 12800.00 0.00 0.11861 19400.00 13000.00 0.00 0.12018 19600.00 13000.00 0.00 0.12134 19800.00 13000.00 0.00 0.12146 19400.00 13200.00 0.00 0.12308 19600.00 13200.00 0.00 0.12423 19800.00 13200.00 0.00 0.12431 19400.00 13400.00 0.00 0.12597 19600.00 13400.00 0.00 0.12708 19800.00 13400.00 0.00 0.12709 19400.00 13600.00 0.00 0.12899 19600.00 13600.00 0.00 0.13003 19400.00 13800.00 0.00 0.13220 19600.00 13800.00 0.00 0.13316 19200.00 14000.00 0.00 0.13334 19400.00 14000.00 0.00 0.13554 19600.00 14000.00 0.00 0.13642 19200.00 14200.00 0.00 0.13679 19400.00 14200.00 0.00 0.13895 19600.00 14200.00 0.00 0.13977 19200.00 14400.00 0.00 0.14028 19400.00 14400.00 0.00 0.14239 19600.00 14400.00 0.00 0.14317 19200.00 14600.00 0.00 0.14368 19400.00 14600.00 0.00 0.14576 19600.00 14600.00 0.00 0.14649 19200.00 14800.00 0.00 0.14710 19400.00 14800.00 0.00 0.14915 19600.00 14800.00 0.00 0.14986 19200.00 15000.00 0.00 0.15074 19400.00 15000.00 0.00 0.15277 19600.00 15000.00 0.00 0.15345 19200.00 15200.00 0.00 0.15455 19400.00 15200.00 0.00 0.15657 19600.00 15200.00 0.00 0.15721 19200.00 15400.00 0.00 0.15845 19400.00 15400.00 0.00 0.16045 19600.00 15400.00 0.00 0.16107 19200.00 15600.00 0.00 0.16242 19400.00 15600.00 0.00 0.16442 19600.00 15600.00 0.00 0.16501 19200.00 15800.00 0.00 0.16640 19400.00 15800.00 0.00 0.16839 19600.00 15800.00 0.00 0.16893 19200.00 16000.00 0.00 0.17036 19400.00 16000.00 0.00 0.17232 19600.00 16000.00 0.00 0.17280 19200.00 16200.00 0.00 0.17449 19400.00 16200.00 0.00 0.17641 19600.00 16200.00 0.00 0.17681 19200.00 16400.00 0.00 0.17883 19400.00 16400.00 0.00 0.18068 19600.00 16400.00 0.00 0.18097 19200.00 16600.00 0.00 0.18318 19400.00 16600.00 0.00 0.18495 19600.00 16600.00 0.00 0.18511 19200.00 16800.00 0.00 0.18751 19400.00 16800.00 0.00 0.18918 19600.00 16800.00 0.00 0.18919 19200.00 17000.00 0.00 0.19177 19400.00 17000.00 0.00 0.19333 19200.00 17200.00 0.00 0.19594 19400.00 17200.00 0.00 0.19739 19200.00 17400.00 0.00 0.20030 19400.00 17400.00 0.00 0.20163 19000.00 17600.00 0.00 0.20211 19200.00 17600.00 0.00 0.20507 19400.00 17600.00 0.00 0.20625 19000.00 17800.00 0.00 0.20726 19200.00 17800.00 0.00 0.21011 19400.00 17800.00 0.00 0.21110 19000.00 18000.00 0.00 0.21260 19200.00 18000.00 0.00 0.21529 19400.00 18000.00 0.00 0.21602 19000.00 18200.00 0.00 0.21801 19200.00 18200.00 0.00 0.22045 19400.00 18200.00 0.00 0.22083 19000.00 18400.00 0.00 0.22301 19200.00 18400.00 0.00 0.22512 19000.00 18600.00 0.00 0.22734 19200.00 18600.00 0.00 0.22904 19000.00 18800.00 0.00 0.23095 19200.00 18800.00 0.00 0.23210 19000.00 19000.00 0.00 0.23319 19200.00 19000.00 0.00 0.23362 19000.00 19200.00 25.00 0.23369 19000.00 19200.00 30.00 0.23377 19000.00 19200.00 35.00 0.23384 19000.00 19200.00 40.00 0.23390 19000.00 19200.00 45.00 0.23394 19000.00 19200.00 50.00 0.23397 19000.00 19200.00 55.00 0.23398 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19013.62 19156.07 55.00 0.23404 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/254.ctf NX, NY, NZ: 360 360 1 MODE : real