CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/257.mrc Output diagnostic file name Micrographs/257.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/257.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2398.000 7704.000 Mean, RMS : 4455.202 225.1161 TITLE 1: emi2mrc: 257.emi Sun Jan 19 22:45:38 2014 TITLE 2: kV 300, Mag 78000x, Def 21330, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -473.415, Y 542.217, Z -22.843, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01688 8200.00 8000.00 0.00 -0.01679 8400.00 8000.00 0.00 -0.01666 8600.00 8000.00 0.00 -0.01650 8800.00 8000.00 0.00 -0.01634 9000.00 8000.00 0.00 -0.01611 9200.00 8000.00 0.00 -0.01576 9400.00 8000.00 0.00 -0.01532 9600.00 8000.00 0.00 -0.01484 9800.00 8000.00 0.00 -0.01435 10000.00 8000.00 0.00 -0.01392 10200.00 8000.00 0.00 -0.01351 10400.00 8000.00 0.00 -0.01307 10600.00 8000.00 0.00 -0.01255 10800.00 8000.00 0.00 -0.01195 11000.00 8000.00 0.00 -0.01127 11200.00 8000.00 0.00 -0.01057 11400.00 8000.00 0.00 -0.00988 11600.00 8000.00 0.00 -0.00919 11800.00 8000.00 0.00 -0.00844 12000.00 8000.00 0.00 -0.00762 12200.00 8000.00 0.00 -0.00670 12400.00 8000.00 0.00 -0.00570 12600.00 8000.00 0.00 -0.00463 12800.00 8000.00 0.00 -0.00350 13000.00 8000.00 0.00 -0.00227 13200.00 8000.00 0.00 -0.00092 13400.00 8000.00 0.00 0.00059 13600.00 8000.00 0.00 0.00225 13800.00 8000.00 0.00 0.00404 14000.00 8000.00 0.00 0.00592 14200.00 8000.00 0.00 0.00785 14400.00 8000.00 0.00 0.00980 14600.00 8000.00 0.00 0.01176 14800.00 8000.00 0.00 0.01374 15000.00 8000.00 0.00 0.01574 15200.00 8000.00 0.00 0.01778 15400.00 8000.00 0.00 0.01985 15600.00 8000.00 0.00 0.02196 15800.00 8000.00 0.00 0.02411 16000.00 8000.00 0.00 0.02633 16200.00 8000.00 0.00 0.02864 16400.00 8000.00 0.00 0.03108 16600.00 8000.00 0.00 0.03362 16800.00 8000.00 0.00 0.03622 17000.00 8000.00 0.00 0.03883 17200.00 8000.00 0.00 0.04140 17400.00 8000.00 0.00 0.04393 17600.00 8000.00 0.00 0.04648 17800.00 8000.00 0.00 0.04908 18000.00 8000.00 0.00 0.05173 18200.00 8000.00 0.00 0.05443 18400.00 8000.00 0.00 0.05718 18600.00 8000.00 0.00 0.06001 18800.00 8000.00 0.00 0.06303 19000.00 8000.00 0.00 0.06627 19200.00 8000.00 0.00 0.06963 19400.00 8000.00 0.00 0.07291 19600.00 8000.00 0.00 0.07584 19800.00 8000.00 0.00 0.07817 20000.00 8000.00 0.00 0.07984 20200.00 8000.00 0.00 0.08090 20400.00 8000.00 0.00 0.08153 20600.00 8000.00 0.00 0.08189 20800.00 8000.00 0.00 0.08211 21000.00 8000.00 0.00 0.08216 20400.00 8200.00 0.00 0.08231 20600.00 8200.00 0.00 0.08266 20800.00 8200.00 0.00 0.08286 21000.00 8200.00 0.00 0.08289 20400.00 8400.00 0.00 0.08311 20600.00 8400.00 0.00 0.08345 20800.00 8400.00 0.00 0.08363 21000.00 8400.00 0.00 0.08363 20400.00 8600.00 0.00 0.08406 20600.00 8600.00 0.00 0.08439 20800.00 8600.00 0.00 0.08455 20200.00 8800.00 0.00 0.08456 20400.00 8800.00 0.00 0.08515 20600.00 8800.00 0.00 0.08546 20800.00 8800.00 0.00 0.08560 20200.00 9000.00 0.00 0.08570 20400.00 9000.00 0.00 0.08628 20600.00 9000.00 0.00 0.08656 20800.00 9000.00 0.00 0.08668 20200.00 9200.00 0.00 0.08686 20400.00 9200.00 0.00 0.08742 20600.00 9200.00 0.00 0.08769 20800.00 9200.00 0.00 0.08777 20200.00 9400.00 0.00 0.08803 20400.00 9400.00 0.00 0.08857 20600.00 9400.00 0.00 0.08880 20800.00 9400.00 0.00 0.08886 20200.00 9600.00 0.00 0.08919 20400.00 9600.00 0.00 0.08970 20600.00 9600.00 0.00 0.08990 20800.00 9600.00 0.00 0.08991 20200.00 9800.00 0.00 0.09044 20400.00 9800.00 0.00 0.09091 20600.00 9800.00 0.00 0.09107 20200.00 10000.00 0.00 0.09178 20400.00 10000.00 0.00 0.09221 20600.00 10000.00 0.00 0.09235 20200.00 10200.00 0.00 0.09309 20400.00 10200.00 0.00 0.09351 20600.00 10200.00 0.00 0.09363 20200.00 10400.00 0.00 0.09435 20400.00 10400.00 0.00 0.09477 20600.00 10400.00 0.00 0.09489 20200.00 10600.00 0.00 0.09563 20400.00 10600.00 0.00 0.09605 20600.00 10600.00 0.00 0.09617 20200.00 10800.00 0.00 0.09698 20400.00 10800.00 0.00 0.09741 20600.00 10800.00 0.00 0.09753 20000.00 11000.00 0.00 0.09758 20200.00 11000.00 0.00 0.09854 20400.00 11000.00 0.00 0.09898 20600.00 11000.00 0.00 0.09908 20000.00 11200.00 0.00 0.09945 20200.00 11200.00 0.00 0.10041 20400.00 11200.00 0.00 0.10083 20600.00 11200.00 0.00 0.10092 20000.00 11400.00 0.00 0.10150 20200.00 11400.00 0.00 0.10245 20400.00 11400.00 0.00 0.10285 20600.00 11400.00 0.00 0.10292 20000.00 11600.00 0.00 0.10356 20200.00 11600.00 0.00 0.10450 20400.00 11600.00 0.00 0.10487 20600.00 11600.00 0.00 0.10492 20000.00 11800.00 0.00 0.10559 20200.00 11800.00 0.00 0.10650 20400.00 11800.00 0.00 0.10685 20600.00 11800.00 0.00 0.10687 20000.00 12000.00 0.00 0.10755 20200.00 12000.00 0.00 0.10845 20400.00 12000.00 0.00 0.10878 20600.00 12000.00 0.00 0.10880 20000.00 12200.00 0.00 0.10959 20200.00 12200.00 0.00 0.11048 20400.00 12200.00 0.00 0.11081 20600.00 12200.00 0.00 0.11083 20000.00 12400.00 0.00 0.11190 20200.00 12400.00 0.00 0.11280 20400.00 12400.00 0.00 0.11314 20600.00 12400.00 0.00 0.11317 20000.00 12600.00 0.00 0.11445 20200.00 12600.00 0.00 0.11537 20400.00 12600.00 0.00 0.11571 20600.00 12600.00 0.00 0.11576 20000.00 12800.00 0.00 0.11706 20200.00 12800.00 0.00 0.11799 20400.00 12800.00 0.00 0.11835 20600.00 12800.00 0.00 0.11839 20000.00 13000.00 0.00 0.11961 20200.00 13000.00 0.00 0.12054 20400.00 13000.00 0.00 0.12089 20600.00 13000.00 0.00 0.12092 20000.00 13200.00 0.00 0.12199 20200.00 13200.00 0.00 0.12292 20400.00 13200.00 0.00 0.12324 20600.00 13200.00 0.00 0.12324 20000.00 13400.00 0.00 0.12424 20200.00 13400.00 0.00 0.12514 20400.00 13400.00 0.00 0.12544 20000.00 13600.00 0.00 0.12660 20200.00 13600.00 0.00 0.12748 20400.00 13600.00 0.00 0.12775 20000.00 13800.00 0.00 0.12923 20200.00 13800.00 0.00 0.13009 20400.00 13800.00 0.00 0.13031 20000.00 14000.00 0.00 0.13203 20200.00 14000.00 0.00 0.13286 20400.00 14000.00 0.00 0.13302 19800.00 14200.00 0.00 0.13319 20000.00 14200.00 0.00 0.13490 20200.00 14200.00 0.00 0.13567 20400.00 14200.00 0.00 0.13578 19800.00 14400.00 0.00 0.13608 20000.00 14400.00 0.00 0.13776 20200.00 14400.00 0.00 0.13847 20400.00 14400.00 0.00 0.13851 19800.00 14600.00 0.00 0.13891 20000.00 14600.00 0.00 0.14055 20200.00 14600.00 0.00 0.14121 19800.00 14800.00 0.00 0.14188 20000.00 14800.00 0.00 0.14349 20200.00 14800.00 0.00 0.14409 19800.00 15000.00 0.00 0.14523 20000.00 15000.00 0.00 0.14679 20200.00 15000.00 0.00 0.14734 19800.00 15200.00 0.00 0.14886 20000.00 15200.00 0.00 0.15038 20200.00 15200.00 0.00 0.15086 19800.00 15400.00 0.00 0.15258 20000.00 15400.00 0.00 0.15404 20200.00 15400.00 0.00 0.15444 19800.00 15600.00 0.00 0.15627 20000.00 15600.00 0.00 0.15766 20200.00 15600.00 0.00 0.15798 19800.00 15800.00 0.00 0.15979 20000.00 15800.00 0.00 0.16112 20200.00 15800.00 0.00 0.16135 19800.00 16000.00 0.00 0.16322 20000.00 16000.00 0.00 0.16447 20200.00 16000.00 0.00 0.16462 19800.00 16200.00 0.00 0.16688 20000.00 16200.00 0.00 0.16804 20200.00 16200.00 0.00 0.16811 19600.00 16400.00 0.00 0.16830 19800.00 16400.00 0.00 0.17086 20000.00 16400.00 0.00 0.17194 19600.00 16600.00 0.00 0.17246 19800.00 16600.00 0.00 0.17497 20000.00 16600.00 0.00 0.17595 19600.00 16800.00 0.00 0.17661 19800.00 16800.00 0.00 0.17905 20000.00 16800.00 0.00 0.17993 19600.00 17000.00 0.00 0.18058 19800.00 17000.00 0.00 0.18296 20000.00 17000.00 0.00 0.18374 19600.00 17200.00 0.00 0.18432 19800.00 17200.00 0.00 0.18664 20000.00 17200.00 0.00 0.18732 19600.00 17400.00 0.00 0.18816 19800.00 17400.00 0.00 0.19041 20000.00 17400.00 0.00 0.19097 19600.00 17600.00 0.00 0.19238 19800.00 17600.00 0.00 0.19456 20000.00 17600.00 0.00 0.19498 19600.00 17800.00 0.00 0.19688 19800.00 17800.00 0.00 0.19896 20000.00 17800.00 0.00 0.19921 19600.00 18000.00 0.00 0.20151 19800.00 18000.00 0.00 0.20347 20000.00 18000.00 0.00 0.20351 19600.00 18200.00 0.00 0.20617 19800.00 18200.00 0.00 0.20797 19600.00 18400.00 0.00 0.21069 19800.00 18400.00 0.00 0.21230 19600.00 18600.00 0.00 0.21530 19800.00 18600.00 0.00 0.21663 19400.00 18800.00 0.00 0.21685 19600.00 18800.00 0.00 0.22033 19800.00 18800.00 0.00 0.22124 19400.00 19000.00 0.00 0.22235 19600.00 19000.00 0.00 0.22541 19800.00 19000.00 0.00 0.22571 19400.00 19200.00 0.00 0.22724 19600.00 19200.00 0.00 0.22969 19400.00 19400.00 0.00 0.23078 19600.00 19400.00 0.00 0.23243 19600.00 19600.00 0.00 0.23276 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19513.31 19572.25 56.67 0.23307 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/257.ctf NX, NY, NZ: 360 360 1 MODE : real