CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/261.mrc Output diagnostic file name Micrographs/261.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/261.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2562.000 8466.000 Mean, RMS : 4454.513 224.1011 TITLE 1: emi2mrc: 261.emi Sun Jan 19 22:48:11 2014 TITLE 2: kV 300, Mag 78000x, Def 21330, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -480.166, Y 533.638, Z -22.843, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.02315 9200.00 8000.00 0.00 -0.02310 9400.00 8000.00 0.00 -0.02287 9600.00 8000.00 0.00 -0.02246 9800.00 8000.00 0.00 -0.02194 10000.00 8000.00 0.00 -0.02141 10200.00 8000.00 0.00 -0.02095 10400.00 8000.00 0.00 -0.02055 10600.00 8000.00 0.00 -0.02020 10800.00 8000.00 0.00 -0.01984 11000.00 8000.00 0.00 -0.01940 11200.00 8000.00 0.00 -0.01887 11400.00 8000.00 0.00 -0.01825 11600.00 8000.00 0.00 -0.01751 11800.00 8000.00 0.00 -0.01670 12000.00 8000.00 0.00 -0.01585 12200.00 8000.00 0.00 -0.01503 12400.00 8000.00 0.00 -0.01426 12600.00 8000.00 0.00 -0.01354 12800.00 8000.00 0.00 -0.01280 13000.00 8000.00 0.00 -0.01196 13200.00 8000.00 0.00 -0.01096 13400.00 8000.00 0.00 -0.00980 13600.00 8000.00 0.00 -0.00851 13800.00 8000.00 0.00 -0.00713 14000.00 8000.00 0.00 -0.00566 14200.00 8000.00 0.00 -0.00407 14400.00 8000.00 0.00 -0.00233 14600.00 8000.00 0.00 -0.00043 14800.00 8000.00 0.00 0.00157 15000.00 8000.00 0.00 0.00359 15200.00 8000.00 0.00 0.00556 15400.00 8000.00 0.00 0.00743 15600.00 8000.00 0.00 0.00925 15800.00 8000.00 0.00 0.01108 16000.00 8000.00 0.00 0.01298 16200.00 8000.00 0.00 0.01501 16400.00 8000.00 0.00 0.01718 16600.00 8000.00 0.00 0.01948 16800.00 8000.00 0.00 0.02186 17000.00 8000.00 0.00 0.02429 17200.00 8000.00 0.00 0.02672 17400.00 8000.00 0.00 0.02915 17600.00 8000.00 0.00 0.03162 17800.00 8000.00 0.00 0.03417 18000.00 8000.00 0.00 0.03684 18200.00 8000.00 0.00 0.03965 18400.00 8000.00 0.00 0.04257 18600.00 8000.00 0.00 0.04555 18800.00 8000.00 0.00 0.04858 19000.00 8000.00 0.00 0.05166 19200.00 8000.00 0.00 0.05480 19400.00 8000.00 0.00 0.05808 19600.00 8000.00 0.00 0.06148 19800.00 8000.00 0.00 0.06496 20000.00 8000.00 0.00 0.06842 20200.00 8000.00 0.00 0.07166 20400.00 8000.00 0.00 0.07447 20600.00 8000.00 0.00 0.07668 20800.00 8000.00 0.00 0.07819 21000.00 8000.00 0.00 0.07901 21200.00 8000.00 0.00 0.07927 21000.00 8200.00 0.00 0.07976 21200.00 8200.00 0.00 0.08001 21000.00 8400.00 0.00 0.08050 21200.00 8400.00 0.00 0.08073 21000.00 8600.00 0.00 0.08133 21200.00 8600.00 0.00 0.08156 21000.00 8800.00 0.00 0.08223 21200.00 8800.00 0.00 0.08246 21000.00 9000.00 0.00 0.08312 21200.00 9000.00 0.00 0.08336 21000.00 9200.00 0.00 0.08401 21200.00 9200.00 0.00 0.08425 21000.00 9400.00 0.00 0.08491 21200.00 9400.00 0.00 0.08515 21000.00 9600.00 0.00 0.08587 21200.00 9600.00 0.00 0.08612 20800.00 9800.00 0.00 0.08623 21000.00 9800.00 0.00 0.08706 21200.00 9800.00 0.00 0.08730 20800.00 10000.00 0.00 0.08767 21000.00 10000.00 0.00 0.08849 21200.00 10000.00 0.00 0.08872 20800.00 10200.00 0.00 0.08922 21000.00 10200.00 0.00 0.09004 21200.00 10200.00 0.00 0.09024 20800.00 10400.00 0.00 0.09079 21000.00 10400.00 0.00 0.09159 21200.00 10400.00 0.00 0.09175 20800.00 10600.00 0.00 0.09233 21000.00 10600.00 0.00 0.09309 21200.00 10600.00 0.00 0.09322 20800.00 10800.00 0.00 0.09379 21000.00 10800.00 0.00 0.09452 21200.00 10800.00 0.00 0.09462 20800.00 11000.00 0.00 0.09528 21000.00 11000.00 0.00 0.09598 21200.00 11000.00 0.00 0.09605 20800.00 11200.00 0.00 0.09691 21000.00 11200.00 0.00 0.09759 21200.00 11200.00 0.00 0.09762 20800.00 11400.00 0.00 0.09862 21000.00 11400.00 0.00 0.09927 20800.00 11600.00 0.00 0.10032 21000.00 11600.00 0.00 0.10094 20800.00 11800.00 0.00 0.10198 21000.00 11800.00 0.00 0.10257 20800.00 12000.00 0.00 0.10361 21000.00 12000.00 0.00 0.10418 20800.00 12200.00 0.00 0.10530 21000.00 12200.00 0.00 0.10585 20800.00 12400.00 0.00 0.10718 21000.00 12400.00 0.00 0.10772 20600.00 12600.00 0.00 0.10785 20800.00 12600.00 0.00 0.10922 21000.00 12600.00 0.00 0.10974 20600.00 12800.00 0.00 0.10989 20800.00 12800.00 0.00 0.11126 21000.00 12800.00 0.00 0.11177 20600.00 13000.00 0.00 0.11189 20800.00 13000.00 0.00 0.11326 21000.00 13000.00 0.00 0.11375 20600.00 13200.00 0.00 0.11382 20800.00 13200.00 0.00 0.11520 21000.00 13200.00 0.00 0.11568 20600.00 13400.00 0.00 0.11577 20800.00 13400.00 0.00 0.11715 21000.00 13400.00 0.00 0.11761 20600.00 13600.00 0.00 0.11796 20800.00 13600.00 0.00 0.11934 21000.00 13600.00 0.00 0.11976 20600.00 13800.00 0.00 0.12047 20800.00 13800.00 0.00 0.12182 21000.00 13800.00 0.00 0.12220 20600.00 14000.00 0.00 0.12310 20800.00 14000.00 0.00 0.12440 21000.00 14000.00 0.00 0.12472 20600.00 14200.00 0.00 0.12566 20800.00 14200.00 0.00 0.12692 21000.00 14200.00 0.00 0.12718 20600.00 14400.00 0.00 0.12803 20800.00 14400.00 0.00 0.12924 21000.00 14400.00 0.00 0.12945 20600.00 14600.00 0.00 0.13013 20800.00 14600.00 0.00 0.13130 21000.00 14600.00 0.00 0.13145 20600.00 14800.00 0.00 0.13213 20800.00 14800.00 0.00 0.13326 21000.00 14800.00 0.00 0.13336 20600.00 15000.00 0.00 0.13429 20800.00 15000.00 0.00 0.13538 21000.00 15000.00 0.00 0.13543 20600.00 15200.00 0.00 0.13662 20800.00 15200.00 0.00 0.13769 21000.00 15200.00 0.00 0.13771 20600.00 15400.00 0.00 0.13908 20800.00 15400.00 0.00 0.14014 20600.00 15600.00 0.00 0.14165 20800.00 15600.00 0.00 0.14271 20600.00 15800.00 0.00 0.14424 20800.00 15800.00 0.00 0.14529 20600.00 16000.00 0.00 0.14688 20800.00 16000.00 0.00 0.14790 20600.00 16200.00 0.00 0.14977 20800.00 16200.00 0.00 0.15075 20400.00 16400.00 0.00 0.15085 20600.00 16400.00 0.00 0.15294 20800.00 16400.00 0.00 0.15387 20400.00 16600.00 0.00 0.15414 20600.00 16600.00 0.00 0.15619 20800.00 16600.00 0.00 0.15708 20400.00 16800.00 0.00 0.15744 20600.00 16800.00 0.00 0.15946 20800.00 16800.00 0.00 0.16029 20400.00 17000.00 0.00 0.16067 20600.00 17000.00 0.00 0.16266 20800.00 17000.00 0.00 0.16343 20400.00 17200.00 0.00 0.16377 20600.00 17200.00 0.00 0.16573 20800.00 17200.00 0.00 0.16644 20400.00 17400.00 0.00 0.16696 20600.00 17400.00 0.00 0.16889 20800.00 17400.00 0.00 0.16954 20400.00 17600.00 0.00 0.17046 20600.00 17600.00 0.00 0.17234 20800.00 17600.00 0.00 0.17290 20400.00 17800.00 0.00 0.17411 20600.00 17800.00 0.00 0.17592 20800.00 17800.00 0.00 0.17638 20400.00 18000.00 0.00 0.17778 20600.00 18000.00 0.00 0.17949 20800.00 18000.00 0.00 0.17981 20400.00 18200.00 0.00 0.18134 20600.00 18200.00 0.00 0.18295 20800.00 18200.00 0.00 0.18310 20400.00 18400.00 0.00 0.18468 20600.00 18400.00 0.00 0.18615 20400.00 18600.00 0.00 0.18794 20600.00 18600.00 0.00 0.18925 20400.00 18800.00 0.00 0.19147 20600.00 18800.00 0.00 0.19260 20200.00 19000.00 0.00 0.19278 20400.00 19000.00 0.00 0.19526 20600.00 19000.00 0.00 0.19618 20200.00 19200.00 0.00 0.19683 20400.00 19200.00 0.00 0.19912 20600.00 19200.00 0.00 0.19981 20200.00 19400.00 0.00 0.20084 20400.00 19400.00 0.00 0.20290 20600.00 19400.00 0.00 0.20331 20200.00 19600.00 0.00 0.20441 20400.00 19600.00 0.00 0.20619 20600.00 19600.00 0.00 0.20628 20200.00 19800.00 0.00 0.20720 20400.00 19800.00 0.00 0.20871 20200.00 20000.00 0.00 0.20943 20400.00 20000.00 0.00 0.21063 20200.00 20200.00 0.00 0.21111 20400.00 20200.00 0.00 0.21189 20400.00 20400.00 0.00 0.21200 20200.00 20400.00 25.00 0.21202 20200.00 20400.00 30.00 0.21207 20200.00 20400.00 35.00 0.21212 20200.00 20400.00 40.00 0.21215 20200.00 20400.00 45.00 0.21218 20200.00 20400.00 50.00 0.21219 20200.00 20400.00 55.00 0.21219 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20276.02 20414.31 46.31 0.21233 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/261.ctf NX, NY, NZ: 360 360 1 MODE : real