CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/264.mrc Output diagnostic file name Micrographs/264.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/264.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2426.000 7862.000 Mean, RMS : 4455.997 223.7226 TITLE 1: emi2mrc: 264.emi Sun Jan 19 22:51:14 2014 TITLE 2: kV 300, Mag 78000x, Def 21330, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -486.684, Y 525.760, Z -22.843, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01205 8200.00 8000.00 0.00 -0.01181 8400.00 8000.00 0.00 -0.01150 8600.00 8000.00 0.00 -0.01122 8800.00 8000.00 0.00 -0.01098 9000.00 8000.00 0.00 -0.01072 9200.00 8000.00 0.00 -0.01038 9400.00 8000.00 0.00 -0.00998 9600.00 8000.00 0.00 -0.00958 9800.00 8000.00 0.00 -0.00928 10000.00 8000.00 0.00 -0.00907 10200.00 8000.00 0.00 -0.00891 10400.00 8000.00 0.00 -0.00865 10600.00 8000.00 0.00 -0.00823 10800.00 8000.00 0.00 -0.00764 11000.00 8000.00 0.00 -0.00694 11200.00 8000.00 0.00 -0.00621 11400.00 8000.00 0.00 -0.00547 11600.00 8000.00 0.00 -0.00466 11800.00 8000.00 0.00 -0.00371 12000.00 8000.00 0.00 -0.00259 12200.00 8000.00 0.00 -0.00131 12400.00 8000.00 0.00 0.00003 12600.00 8000.00 0.00 0.00138 12800.00 8000.00 0.00 0.00270 13000.00 8000.00 0.00 0.00404 13200.00 8000.00 0.00 0.00543 13400.00 8000.00 0.00 0.00690 13600.00 8000.00 0.00 0.00847 13800.00 8000.00 0.00 0.01014 14000.00 8000.00 0.00 0.01193 14200.00 8000.00 0.00 0.01387 14400.00 8000.00 0.00 0.01598 14600.00 8000.00 0.00 0.01827 14800.00 8000.00 0.00 0.02069 15000.00 8000.00 0.00 0.02319 15200.00 8000.00 0.00 0.02570 15400.00 8000.00 0.00 0.02820 15600.00 8000.00 0.00 0.03069 15800.00 8000.00 0.00 0.03320 16000.00 8000.00 0.00 0.03574 16200.00 8000.00 0.00 0.03837 16400.00 8000.00 0.00 0.04113 16600.00 8000.00 0.00 0.04402 16800.00 8000.00 0.00 0.04700 17000.00 8000.00 0.00 0.05002 17200.00 8000.00 0.00 0.05302 17400.00 8000.00 0.00 0.05597 17600.00 8000.00 0.00 0.05897 17800.00 8000.00 0.00 0.06212 18000.00 8000.00 0.00 0.06551 18200.00 8000.00 0.00 0.06914 18400.00 8000.00 0.00 0.07290 18600.00 8000.00 0.00 0.07654 18800.00 8000.00 0.00 0.07982 19000.00 8000.00 0.00 0.08251 19200.00 8000.00 0.00 0.08446 19400.00 8000.00 0.00 0.08572 19600.00 8000.00 0.00 0.08643 19800.00 8000.00 0.00 0.08679 20000.00 8000.00 0.00 0.08705 20200.00 8000.00 0.00 0.08729 20400.00 8000.00 0.00 0.08745 19600.00 8200.00 0.00 0.08753 19800.00 8200.00 0.00 0.08787 20000.00 8200.00 0.00 0.08811 20200.00 8200.00 0.00 0.08832 20400.00 8200.00 0.00 0.08845 19600.00 8400.00 0.00 0.08871 19800.00 8400.00 0.00 0.08903 20000.00 8400.00 0.00 0.08923 20200.00 8400.00 0.00 0.08942 20400.00 8400.00 0.00 0.08952 19600.00 8600.00 0.00 0.09009 19800.00 8600.00 0.00 0.09038 20000.00 8600.00 0.00 0.09057 20200.00 8600.00 0.00 0.09073 20400.00 8600.00 0.00 0.09080 19400.00 8800.00 0.00 0.09101 19600.00 8800.00 0.00 0.09160 19800.00 8800.00 0.00 0.09188 20000.00 8800.00 0.00 0.09204 20200.00 8800.00 0.00 0.09218 20400.00 8800.00 0.00 0.09220 19400.00 9000.00 0.00 0.09252 19600.00 9000.00 0.00 0.09309 19800.00 9000.00 0.00 0.09335 20000.00 9000.00 0.00 0.09349 20200.00 9000.00 0.00 0.09359 19400.00 9200.00 0.00 0.09393 19600.00 9200.00 0.00 0.09449 19800.00 9200.00 0.00 0.09473 20000.00 9200.00 0.00 0.09485 20200.00 9200.00 0.00 0.09492 19400.00 9400.00 0.00 0.09525 19600.00 9400.00 0.00 0.09580 19800.00 9400.00 0.00 0.09603 20000.00 9400.00 0.00 0.09614 20200.00 9400.00 0.00 0.09618 19400.00 9600.00 0.00 0.09660 19600.00 9600.00 0.00 0.09714 19800.00 9600.00 0.00 0.09736 20000.00 9600.00 0.00 0.09745 20200.00 9600.00 0.00 0.09747 19400.00 9800.00 0.00 0.09818 19600.00 9800.00 0.00 0.09872 19800.00 9800.00 0.00 0.09894 20000.00 9800.00 0.00 0.09900 19200.00 10000.00 0.00 0.09903 19400.00 10000.00 0.00 0.10009 19600.00 10000.00 0.00 0.10063 19800.00 10000.00 0.00 0.10082 20000.00 10000.00 0.00 0.10085 19200.00 10200.00 0.00 0.10111 19400.00 10200.00 0.00 0.10216 19600.00 10200.00 0.00 0.10267 19800.00 10200.00 0.00 0.10283 19200.00 10400.00 0.00 0.10317 19400.00 10400.00 0.00 0.10419 19600.00 10400.00 0.00 0.10467 19800.00 10400.00 0.00 0.10477 19200.00 10600.00 0.00 0.10508 19400.00 10600.00 0.00 0.10606 19600.00 10600.00 0.00 0.10650 19800.00 10600.00 0.00 0.10655 19200.00 10800.00 0.00 0.10681 19400.00 10800.00 0.00 0.10776 19600.00 10800.00 0.00 0.10815 19800.00 10800.00 0.00 0.10817 19200.00 11000.00 0.00 0.10858 19400.00 11000.00 0.00 0.10950 19600.00 11000.00 0.00 0.10985 19200.00 11200.00 0.00 0.11061 19400.00 11200.00 0.00 0.11150 19600.00 11200.00 0.00 0.11183 19200.00 11400.00 0.00 0.11288 19400.00 11400.00 0.00 0.11374 19600.00 11400.00 0.00 0.11403 19200.00 11600.00 0.00 0.11522 19400.00 11600.00 0.00 0.11606 19600.00 11600.00 0.00 0.11631 19200.00 11800.00 0.00 0.11751 19400.00 11800.00 0.00 0.11832 19600.00 11800.00 0.00 0.11853 19200.00 12000.00 0.00 0.11961 19400.00 12000.00 0.00 0.12039 19600.00 12000.00 0.00 0.12057 19200.00 12200.00 0.00 0.12163 19400.00 12200.00 0.00 0.12238 19600.00 12200.00 0.00 0.12252 19200.00 12400.00 0.00 0.12387 19400.00 12400.00 0.00 0.12459 19600.00 12400.00 0.00 0.12468 19000.00 12600.00 0.00 0.12492 19200.00 12600.00 0.00 0.12645 19400.00 12600.00 0.00 0.12712 19600.00 12600.00 0.00 0.12715 19000.00 12800.00 0.00 0.12777 19200.00 12800.00 0.00 0.12926 19400.00 12800.00 0.00 0.12986 19000.00 13000.00 0.00 0.13076 19200.00 13000.00 0.00 0.13218 19400.00 13000.00 0.00 0.13271 19000.00 13200.00 0.00 0.13367 19200.00 13200.00 0.00 0.13502 19400.00 13200.00 0.00 0.13549 19000.00 13400.00 0.00 0.13642 19200.00 13400.00 0.00 0.13771 19400.00 13400.00 0.00 0.13812 19000.00 13600.00 0.00 0.13923 19200.00 13600.00 0.00 0.14046 19400.00 13600.00 0.00 0.14081 19000.00 13800.00 0.00 0.14229 19200.00 13800.00 0.00 0.14346 19400.00 13800.00 0.00 0.14376 19000.00 14000.00 0.00 0.14556 19200.00 14000.00 0.00 0.14669 19400.00 14000.00 0.00 0.14695 19000.00 14200.00 0.00 0.14902 19200.00 14200.00 0.00 0.15011 19400.00 14200.00 0.00 0.15035 18800.00 14400.00 0.00 0.15044 19000.00 14400.00 0.00 0.15261 19200.00 14400.00 0.00 0.15368 19400.00 14400.00 0.00 0.15390 18800.00 14600.00 0.00 0.15406 19000.00 14600.00 0.00 0.15621 19200.00 14600.00 0.00 0.15726 19400.00 14600.00 0.00 0.15745 18800.00 14800.00 0.00 0.15781 19000.00 14800.00 0.00 0.15993 19200.00 14800.00 0.00 0.16095 19400.00 14800.00 0.00 0.16111 18800.00 15000.00 0.00 0.16191 19000.00 15000.00 0.00 0.16400 19200.00 15000.00 0.00 0.16499 19400.00 15000.00 0.00 0.16510 18800.00 15200.00 0.00 0.16630 19000.00 15200.00 0.00 0.16836 19200.00 15200.00 0.00 0.16929 19400.00 15200.00 0.00 0.16934 18800.00 15400.00 0.00 0.17076 19000.00 15400.00 0.00 0.17277 19200.00 15400.00 0.00 0.17364 18800.00 15600.00 0.00 0.17512 19000.00 15600.00 0.00 0.17709 19200.00 15600.00 0.00 0.17791 18800.00 15800.00 0.00 0.17923 19000.00 15800.00 0.00 0.18116 19200.00 15800.00 0.00 0.18192 18800.00 16000.00 0.00 0.18315 19000.00 16000.00 0.00 0.18503 19200.00 16000.00 0.00 0.18571 18800.00 16200.00 0.00 0.18729 19000.00 16200.00 0.00 0.18910 19200.00 16200.00 0.00 0.18967 18800.00 16400.00 0.00 0.19185 19000.00 16400.00 0.00 0.19355 19200.00 16400.00 0.00 0.19397 18800.00 16600.00 0.00 0.19667 19000.00 16600.00 0.00 0.19824 19200.00 16600.00 0.00 0.19848 18600.00 16800.00 0.00 0.19875 18800.00 16800.00 0.00 0.20158 19000.00 16800.00 0.00 0.20299 19200.00 16800.00 0.00 0.20305 18600.00 17000.00 0.00 0.20365 18800.00 17000.00 0.00 0.20637 19000.00 17000.00 0.00 0.20762 18600.00 17200.00 0.00 0.20835 18800.00 17200.00 0.00 0.21097 19000.00 17200.00 0.00 0.21204 18600.00 17400.00 0.00 0.21340 18800.00 17400.00 0.00 0.21586 19000.00 17400.00 0.00 0.21671 18600.00 17600.00 0.00 0.21934 18800.00 17600.00 0.00 0.22155 19000.00 17600.00 0.00 0.22208 18400.00 17800.00 0.00 0.22230 18600.00 17800.00 0.00 0.22608 18800.00 17800.00 0.00 0.22789 19000.00 17800.00 0.00 0.22797 18400.00 18000.00 0.00 0.22981 18600.00 18000.00 0.00 0.23311 18800.00 18000.00 0.00 0.23433 18400.00 18200.00 0.00 0.23690 18600.00 18200.00 0.00 0.23945 18800.00 18200.00 0.00 0.23989 18400.00 18400.00 0.00 0.24193 18600.00 18400.00 0.00 0.24354 18400.00 18600.00 0.00 0.24380 18600.00 18600.00 0.00 0.24452 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18511.95 18661.41 48.11 0.24481 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/264.ctf NX, NY, NZ: 360 360 1 MODE : real