CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/265.mrc Output diagnostic file name Micrographs/265.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/265.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2364.000 7802.000 Mean, RMS : 4455.014 224.8172 TITLE 1: emi2mrc: 265.emi Sun Jan 19 22:50:27 2014 TITLE 2: kV 300, Mag 78000x, Def 21330, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -484.831, Y 527.905, Z -22.843, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00799 8200.00 8000.00 0.00 -0.00772 8400.00 8000.00 0.00 -0.00734 8600.00 8000.00 0.00 -0.00692 8800.00 8000.00 0.00 -0.00650 9000.00 8000.00 0.00 -0.00606 9200.00 8000.00 0.00 -0.00557 9400.00 8000.00 0.00 -0.00504 9600.00 8000.00 0.00 -0.00444 9800.00 8000.00 0.00 -0.00380 10000.00 8000.00 0.00 -0.00311 10200.00 8000.00 0.00 -0.00236 10400.00 8000.00 0.00 -0.00151 10600.00 8000.00 0.00 -0.00052 10800.00 8000.00 0.00 0.00061 11000.00 8000.00 0.00 0.00186 11200.00 8000.00 0.00 0.00316 11400.00 8000.00 0.00 0.00445 11600.00 8000.00 0.00 0.00573 11800.00 8000.00 0.00 0.00704 12000.00 8000.00 0.00 0.00840 12200.00 8000.00 0.00 0.00986 12400.00 8000.00 0.00 0.01142 12600.00 8000.00 0.00 0.01307 12800.00 8000.00 0.00 0.01477 13000.00 8000.00 0.00 0.01653 13200.00 8000.00 0.00 0.01837 13400.00 8000.00 0.00 0.02029 13600.00 8000.00 0.00 0.02231 13800.00 8000.00 0.00 0.02441 14000.00 8000.00 0.00 0.02658 14200.00 8000.00 0.00 0.02880 14400.00 8000.00 0.00 0.03112 14600.00 8000.00 0.00 0.03359 14800.00 8000.00 0.00 0.03623 15000.00 8000.00 0.00 0.03907 15200.00 8000.00 0.00 0.04206 15400.00 8000.00 0.00 0.04516 15600.00 8000.00 0.00 0.04833 15800.00 8000.00 0.00 0.05157 16000.00 8000.00 0.00 0.05489 16200.00 8000.00 0.00 0.05828 16400.00 8000.00 0.00 0.06171 16600.00 8000.00 0.00 0.06513 16800.00 8000.00 0.00 0.06854 17000.00 8000.00 0.00 0.07206 17200.00 8000.00 0.00 0.07580 17400.00 8000.00 0.00 0.07986 17600.00 8000.00 0.00 0.08419 17800.00 8000.00 0.00 0.08846 18000.00 8000.00 0.00 0.09223 18200.00 8000.00 0.00 0.09511 18400.00 8000.00 0.00 0.09693 18600.00 8000.00 0.00 0.09783 18800.00 8000.00 0.00 0.09821 19000.00 8000.00 0.00 0.09841 19200.00 8000.00 0.00 0.09860 19400.00 8000.00 0.00 0.09875 18600.00 8200.00 0.00 0.09907 18800.00 8200.00 0.00 0.09947 19000.00 8200.00 0.00 0.09970 19200.00 8200.00 0.00 0.09992 19400.00 8200.00 0.00 0.10008 18600.00 8400.00 0.00 0.10035 18800.00 8400.00 0.00 0.10079 19000.00 8400.00 0.00 0.10105 19200.00 8400.00 0.00 0.10129 19400.00 8400.00 0.00 0.10145 18600.00 8600.00 0.00 0.10184 18800.00 8600.00 0.00 0.10231 19000.00 8600.00 0.00 0.10261 19200.00 8600.00 0.00 0.10285 19400.00 8600.00 0.00 0.10301 18600.00 8800.00 0.00 0.10350 18800.00 8800.00 0.00 0.10400 19000.00 8800.00 0.00 0.10432 19200.00 8800.00 0.00 0.10456 19400.00 8800.00 0.00 0.10470 18600.00 9000.00 0.00 0.10522 18800.00 9000.00 0.00 0.10574 19000.00 9000.00 0.00 0.10606 19200.00 9000.00 0.00 0.10630 19400.00 9000.00 0.00 0.10640 18600.00 9200.00 0.00 0.10695 18800.00 9200.00 0.00 0.10748 19000.00 9200.00 0.00 0.10779 19200.00 9200.00 0.00 0.10800 19400.00 9200.00 0.00 0.10804 18600.00 9400.00 0.00 0.10863 18800.00 9400.00 0.00 0.10916 19000.00 9400.00 0.00 0.10945 19200.00 9400.00 0.00 0.10960 18600.00 9600.00 0.00 0.11025 18800.00 9600.00 0.00 0.11075 19000.00 9600.00 0.00 0.11100 19200.00 9600.00 0.00 0.11110 18600.00 9800.00 0.00 0.11191 18800.00 9800.00 0.00 0.11239 19000.00 9800.00 0.00 0.11258 19200.00 9800.00 0.00 0.11260 18400.00 10000.00 0.00 0.11263 18600.00 10000.00 0.00 0.11366 18800.00 10000.00 0.00 0.11409 19000.00 10000.00 0.00 0.11423 18400.00 10200.00 0.00 0.11438 18600.00 10200.00 0.00 0.11539 18800.00 10200.00 0.00 0.11578 19000.00 10200.00 0.00 0.11587 18400.00 10400.00 0.00 0.11612 18600.00 10400.00 0.00 0.11711 18800.00 10400.00 0.00 0.11746 19000.00 10400.00 0.00 0.11751 18400.00 10600.00 0.00 0.11789 18600.00 10600.00 0.00 0.11885 18800.00 10600.00 0.00 0.11918 19000.00 10600.00 0.00 0.11918 18400.00 10800.00 0.00 0.11971 18600.00 10800.00 0.00 0.12066 18800.00 10800.00 0.00 0.12097 18400.00 11000.00 0.00 0.12176 18600.00 11000.00 0.00 0.12270 18800.00 11000.00 0.00 0.12297 18400.00 11200.00 0.00 0.12414 18600.00 11200.00 0.00 0.12505 18800.00 11200.00 0.00 0.12530 18400.00 11400.00 0.00 0.12669 18600.00 11400.00 0.00 0.12758 18800.00 11400.00 0.00 0.12779 18400.00 11600.00 0.00 0.12926 18600.00 11600.00 0.00 0.13012 18800.00 11600.00 0.00 0.13029 18400.00 11800.00 0.00 0.13179 18600.00 11800.00 0.00 0.13262 18800.00 11800.00 0.00 0.13273 18400.00 12000.00 0.00 0.13420 18600.00 12000.00 0.00 0.13499 18800.00 12000.00 0.00 0.13505 18400.00 12200.00 0.00 0.13658 18600.00 12200.00 0.00 0.13732 18400.00 12400.00 0.00 0.13914 18600.00 12400.00 0.00 0.13985 18200.00 12600.00 0.00 0.14008 18400.00 12600.00 0.00 0.14194 18600.00 12600.00 0.00 0.14261 18200.00 12800.00 0.00 0.14301 18400.00 12800.00 0.00 0.14487 18600.00 12800.00 0.00 0.14552 18200.00 13000.00 0.00 0.14608 18400.00 13000.00 0.00 0.14793 18600.00 13000.00 0.00 0.14856 18200.00 13200.00 0.00 0.14924 18400.00 13200.00 0.00 0.15109 18600.00 13200.00 0.00 0.15167 18200.00 13400.00 0.00 0.15247 18400.00 13400.00 0.00 0.15430 18600.00 13400.00 0.00 0.15484 18200.00 13600.00 0.00 0.15594 18400.00 13600.00 0.00 0.15775 18600.00 13600.00 0.00 0.15826 18200.00 13800.00 0.00 0.15976 18400.00 13800.00 0.00 0.16155 18600.00 13800.00 0.00 0.16200 18200.00 14000.00 0.00 0.16374 18400.00 14000.00 0.00 0.16551 18600.00 14000.00 0.00 0.16591 18200.00 14200.00 0.00 0.16782 18400.00 14200.00 0.00 0.16958 18600.00 14200.00 0.00 0.16991 18200.00 14400.00 0.00 0.17203 18400.00 14400.00 0.00 0.17375 18600.00 14400.00 0.00 0.17401 18200.00 14600.00 0.00 0.17628 18400.00 14600.00 0.00 0.17796 18600.00 14600.00 0.00 0.17811 18200.00 14800.00 0.00 0.18067 18400.00 14800.00 0.00 0.18229 18600.00 14800.00 0.00 0.18233 18200.00 15000.00 0.00 0.18540 18400.00 15000.00 0.00 0.18693 18000.00 15200.00 0.00 0.18713 18200.00 15200.00 0.00 0.19031 18400.00 15200.00 0.00 0.19173 18000.00 15400.00 0.00 0.19209 18200.00 15400.00 0.00 0.19521 18400.00 15400.00 0.00 0.19650 18000.00 15600.00 0.00 0.19707 18200.00 15600.00 0.00 0.20010 18400.00 15600.00 0.00 0.20125 18000.00 15800.00 0.00 0.20199 18200.00 15800.00 0.00 0.20493 18400.00 15800.00 0.00 0.20592 18000.00 16000.00 0.00 0.20690 18200.00 16000.00 0.00 0.20973 18400.00 16000.00 0.00 0.21056 18000.00 16200.00 0.00 0.21205 18200.00 16200.00 0.00 0.21477 18400.00 16200.00 0.00 0.21541 18000.00 16400.00 0.00 0.21742 18200.00 16400.00 0.00 0.22002 18400.00 16400.00 0.00 0.22046 18000.00 16600.00 0.00 0.22281 18200.00 16600.00 0.00 0.22528 18400.00 16600.00 0.00 0.22548 18000.00 16800.00 0.00 0.22832 18200.00 16800.00 0.00 0.23062 18000.00 17000.00 0.00 0.23406 18200.00 17000.00 0.00 0.23612 18000.00 17200.00 0.00 0.23997 18200.00 17200.00 0.00 0.24170 17800.00 17400.00 0.00 0.24235 18000.00 17400.00 0.00 0.24612 18200.00 17400.00 0.00 0.24736 17800.00 17600.00 0.00 0.24882 18000.00 17600.00 0.00 0.25204 18200.00 17600.00 0.00 0.25256 17800.00 17800.00 0.00 0.25385 18000.00 17800.00 0.00 0.25624 18000.00 18000.00 0.00 0.25743 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18056.50 17925.78 -35.41 0.25767 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/265.ctf NX, NY, NZ: 360 360 1 MODE : real