CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/268.mrc Output diagnostic file name Micrographs/268.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/268.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2270.000 7868.000 Mean, RMS : 4455.643 223.6944 TITLE 1: emi2mrc: 268.emi Sun Jan 19 22:53:29 2014 TITLE 2: kV 300, Mag 78000x, Def 21330, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -491.420, Y 519.948, Z -22.843, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01962 8200.00 8000.00 0.00 -0.01913 8400.00 8000.00 0.00 -0.01848 8600.00 8000.00 0.00 -0.01783 8800.00 8000.00 0.00 -0.01730 9000.00 8000.00 0.00 -0.01692 9200.00 8000.00 0.00 -0.01667 9400.00 8000.00 0.00 -0.01652 9600.00 8000.00 0.00 -0.01641 9800.00 8000.00 0.00 -0.01630 10000.00 8000.00 0.00 -0.01620 10200.00 8000.00 0.00 -0.01605 10400.00 8000.00 0.00 -0.01583 10600.00 8000.00 0.00 -0.01550 10800.00 8000.00 0.00 -0.01503 11000.00 8000.00 0.00 -0.01442 11200.00 8000.00 0.00 -0.01367 11400.00 8000.00 0.00 -0.01279 11600.00 8000.00 0.00 -0.01175 11800.00 8000.00 0.00 -0.01055 12000.00 8000.00 0.00 -0.00921 12200.00 8000.00 0.00 -0.00777 12400.00 8000.00 0.00 -0.00628 12600.00 8000.00 0.00 -0.00478 12800.00 8000.00 0.00 -0.00328 13000.00 8000.00 0.00 -0.00176 13200.00 8000.00 0.00 -0.00022 13400.00 8000.00 0.00 0.00135 13600.00 8000.00 0.00 0.00298 13800.00 8000.00 0.00 0.00468 14000.00 8000.00 0.00 0.00646 14200.00 8000.00 0.00 0.00834 14400.00 8000.00 0.00 0.01028 14600.00 8000.00 0.00 0.01222 14800.00 8000.00 0.00 0.01411 15000.00 8000.00 0.00 0.01595 15200.00 8000.00 0.00 0.01780 15400.00 8000.00 0.00 0.01974 15600.00 8000.00 0.00 0.02185 15800.00 8000.00 0.00 0.02415 16000.00 8000.00 0.00 0.02658 16200.00 8000.00 0.00 0.02905 16400.00 8000.00 0.00 0.03149 16600.00 8000.00 0.00 0.03386 16800.00 8000.00 0.00 0.03620 17000.00 8000.00 0.00 0.03859 17200.00 8000.00 0.00 0.04106 17400.00 8000.00 0.00 0.04366 17600.00 8000.00 0.00 0.04637 17800.00 8000.00 0.00 0.04915 18000.00 8000.00 0.00 0.05196 18200.00 8000.00 0.00 0.05483 18400.00 8000.00 0.00 0.05779 18600.00 8000.00 0.00 0.06088 18800.00 8000.00 0.00 0.06411 19000.00 8000.00 0.00 0.06739 19200.00 8000.00 0.00 0.07049 19400.00 8000.00 0.00 0.07318 19600.00 8000.00 0.00 0.07528 19800.00 8000.00 0.00 0.07675 20000.00 8000.00 0.00 0.07771 20200.00 8000.00 0.00 0.07836 20400.00 8000.00 0.00 0.07884 20600.00 8000.00 0.00 0.07924 20800.00 8000.00 0.00 0.07955 21000.00 8000.00 0.00 0.07968 20400.00 8200.00 0.00 0.08005 20600.00 8200.00 0.00 0.08045 20800.00 8200.00 0.00 0.08075 21000.00 8200.00 0.00 0.08086 20400.00 8400.00 0.00 0.08121 20600.00 8400.00 0.00 0.08160 20800.00 8400.00 0.00 0.08188 21000.00 8400.00 0.00 0.08197 20400.00 8600.00 0.00 0.08239 20600.00 8600.00 0.00 0.08277 20800.00 8600.00 0.00 0.08303 21000.00 8600.00 0.00 0.08310 20200.00 8800.00 0.00 0.08312 20400.00 8800.00 0.00 0.08358 20600.00 8800.00 0.00 0.08393 20800.00 8800.00 0.00 0.08417 21000.00 8800.00 0.00 0.08420 20200.00 9000.00 0.00 0.08426 20400.00 9000.00 0.00 0.08469 20600.00 9000.00 0.00 0.08502 20800.00 9000.00 0.00 0.08522 21000.00 9000.00 0.00 0.08523 20200.00 9200.00 0.00 0.08534 20400.00 9200.00 0.00 0.08576 20600.00 9200.00 0.00 0.08606 20800.00 9200.00 0.00 0.08624 20200.00 9400.00 0.00 0.08639 20400.00 9400.00 0.00 0.08679 20600.00 9400.00 0.00 0.08707 20800.00 9400.00 0.00 0.08723 20200.00 9600.00 0.00 0.08743 20400.00 9600.00 0.00 0.08782 20600.00 9600.00 0.00 0.08808 20800.00 9600.00 0.00 0.08822 20200.00 9800.00 0.00 0.08861 20400.00 9800.00 0.00 0.08898 20600.00 9800.00 0.00 0.08924 20800.00 9800.00 0.00 0.08936 20000.00 10000.00 0.00 0.08940 20200.00 10000.00 0.00 0.08997 20400.00 10000.00 0.00 0.09034 20600.00 10000.00 0.00 0.09059 20800.00 10000.00 0.00 0.09071 20000.00 10200.00 0.00 0.09086 20200.00 10200.00 0.00 0.09144 20400.00 10200.00 0.00 0.09182 20600.00 10200.00 0.00 0.09208 20800.00 10200.00 0.00 0.09220 20000.00 10400.00 0.00 0.09243 20200.00 10400.00 0.00 0.09302 20400.00 10400.00 0.00 0.09342 20600.00 10400.00 0.00 0.09368 20800.00 10400.00 0.00 0.09379 20000.00 10600.00 0.00 0.09412 20200.00 10600.00 0.00 0.09473 20400.00 10600.00 0.00 0.09514 20600.00 10600.00 0.00 0.09539 20800.00 10600.00 0.00 0.09549 20000.00 10800.00 0.00 0.09593 20200.00 10800.00 0.00 0.09656 20400.00 10800.00 0.00 0.09695 20600.00 10800.00 0.00 0.09719 20800.00 10800.00 0.00 0.09726 20000.00 11000.00 0.00 0.09791 20200.00 11000.00 0.00 0.09853 20400.00 11000.00 0.00 0.09890 20600.00 11000.00 0.00 0.09910 20800.00 11000.00 0.00 0.09914 20000.00 11200.00 0.00 0.10006 20200.00 11200.00 0.00 0.10065 20400.00 11200.00 0.00 0.10098 20600.00 11200.00 0.00 0.10114 20800.00 11200.00 0.00 0.10114 19800.00 11400.00 0.00 0.10126 20000.00 11400.00 0.00 0.10222 20200.00 11400.00 0.00 0.10277 20400.00 11400.00 0.00 0.10305 20600.00 11400.00 0.00 0.10316 19800.00 11600.00 0.00 0.10332 20000.00 11600.00 0.00 0.10424 20200.00 11600.00 0.00 0.10475 20400.00 11600.00 0.00 0.10498 20600.00 11600.00 0.00 0.10505 19800.00 11800.00 0.00 0.10523 20000.00 11800.00 0.00 0.10611 20200.00 11800.00 0.00 0.10657 20400.00 11800.00 0.00 0.10676 20600.00 11800.00 0.00 0.10678 19800.00 12000.00 0.00 0.10699 20000.00 12000.00 0.00 0.10785 20200.00 12000.00 0.00 0.10827 20400.00 12000.00 0.00 0.10841 19800.00 12200.00 0.00 0.10876 20000.00 12200.00 0.00 0.10959 20200.00 12200.00 0.00 0.10999 20400.00 12200.00 0.00 0.11012 19800.00 12400.00 0.00 0.11075 20000.00 12400.00 0.00 0.11158 20200.00 12400.00 0.00 0.11196 20400.00 12400.00 0.00 0.11208 19800.00 12600.00 0.00 0.11299 20000.00 12600.00 0.00 0.11381 20200.00 12600.00 0.00 0.11420 20400.00 12600.00 0.00 0.11431 19800.00 12800.00 0.00 0.11537 20000.00 12800.00 0.00 0.11620 20200.00 12800.00 0.00 0.11658 20400.00 12800.00 0.00 0.11669 19800.00 13000.00 0.00 0.11784 20000.00 13000.00 0.00 0.11868 20200.00 13000.00 0.00 0.11905 20400.00 13000.00 0.00 0.11913 19800.00 13200.00 0.00 0.12035 20000.00 13200.00 0.00 0.12117 20200.00 13200.00 0.00 0.12150 20400.00 13200.00 0.00 0.12154 19800.00 13400.00 0.00 0.12286 20000.00 13400.00 0.00 0.12364 20200.00 13400.00 0.00 0.12392 19600.00 13600.00 0.00 0.12398 19800.00 13600.00 0.00 0.12548 20000.00 13600.00 0.00 0.12621 20200.00 13600.00 0.00 0.12644 19600.00 13800.00 0.00 0.12682 19800.00 13800.00 0.00 0.12829 20000.00 13800.00 0.00 0.12896 20200.00 13800.00 0.00 0.12913 19600.00 14000.00 0.00 0.12972 19800.00 14000.00 0.00 0.13115 20000.00 14000.00 0.00 0.13178 20200.00 14000.00 0.00 0.13188 19600.00 14200.00 0.00 0.13264 19800.00 14200.00 0.00 0.13403 20000.00 14200.00 0.00 0.13460 20200.00 14200.00 0.00 0.13464 19600.00 14400.00 0.00 0.13558 19800.00 14400.00 0.00 0.13693 20000.00 14400.00 0.00 0.13744 19600.00 14600.00 0.00 0.13850 19800.00 14600.00 0.00 0.13982 20000.00 14600.00 0.00 0.14028 19600.00 14800.00 0.00 0.14156 19800.00 14800.00 0.00 0.14285 20000.00 14800.00 0.00 0.14325 19600.00 15000.00 0.00 0.14489 19800.00 15000.00 0.00 0.14614 20000.00 15000.00 0.00 0.14649 19600.00 15200.00 0.00 0.14835 19800.00 15200.00 0.00 0.14956 20000.00 15200.00 0.00 0.14984 19600.00 15400.00 0.00 0.15175 19800.00 15400.00 0.00 0.15292 20000.00 15400.00 0.00 0.15312 19600.00 15600.00 0.00 0.15510 19800.00 15600.00 0.00 0.15622 20000.00 15600.00 0.00 0.15634 19600.00 15800.00 0.00 0.15840 19800.00 15800.00 0.00 0.15945 20000.00 15800.00 0.00 0.15948 19400.00 16000.00 0.00 0.15950 19600.00 16000.00 0.00 0.16174 19800.00 16000.00 0.00 0.16272 19400.00 16200.00 0.00 0.16321 19600.00 16200.00 0.00 0.16539 19800.00 16200.00 0.00 0.16629 19400.00 16400.00 0.00 0.16723 19600.00 16400.00 0.00 0.16935 19800.00 16400.00 0.00 0.17015 19400.00 16600.00 0.00 0.17128 19600.00 16600.00 0.00 0.17333 19800.00 16600.00 0.00 0.17401 19400.00 16800.00 0.00 0.17520 19600.00 16800.00 0.00 0.17717 19800.00 16800.00 0.00 0.17775 19400.00 17000.00 0.00 0.17892 19600.00 17000.00 0.00 0.18082 19800.00 17000.00 0.00 0.18128 19400.00 17200.00 0.00 0.18245 19600.00 17200.00 0.00 0.18428 19800.00 17200.00 0.00 0.18464 19400.00 17400.00 0.00 0.18611 19600.00 17400.00 0.00 0.18787 19800.00 17400.00 0.00 0.18813 19400.00 17600.00 0.00 0.19014 19600.00 17600.00 0.00 0.19182 19800.00 17600.00 0.00 0.19194 19400.00 17800.00 0.00 0.19437 19600.00 17800.00 0.00 0.19594 19400.00 18000.00 0.00 0.19863 19600.00 18000.00 0.00 0.20006 19400.00 18200.00 0.00 0.20287 19600.00 18200.00 0.00 0.20410 19200.00 18400.00 0.00 0.20427 19400.00 18400.00 0.00 0.20694 19600.00 18400.00 0.00 0.20792 19200.00 18600.00 0.00 0.20851 19400.00 18600.00 0.00 0.21097 19600.00 18600.00 0.00 0.21162 19200.00 18800.00 0.00 0.21300 19400.00 18800.00 0.00 0.21511 19600.00 18800.00 0.00 0.21530 19200.00 19000.00 0.00 0.21714 19400.00 19000.00 0.00 0.21874 19200.00 19200.00 0.00 0.21992 19400.00 19200.00 0.00 0.22087 19400.00 19400.00 0.00 0.22099 19200.00 19400.00 15.00 0.22103 19200.00 19400.00 20.00 0.22112 19200.00 19400.00 25.00 0.22119 19200.00 19400.00 30.00 0.22126 19200.00 19400.00 35.00 0.22131 19200.00 19400.00 40.00 0.22135 19200.00 19400.00 45.00 0.22137 19200.00 19400.00 50.00 0.22137 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19259.04 19444.40 50.00 0.22158 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/268.ctf NX, NY, NZ: 360 360 1 MODE : real