CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/272.mrc Output diagnostic file name Micrographs/272.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/272.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2016.000 7994.000 Mean, RMS : 4454.849 220.0023 TITLE 1: emi2mrc: 272.emi Sun Jan 19 22:56:46 2014 TITLE 2: kV 300, Mag 78000x, Def 21330, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -482.374, Y 526.819, Z -22.843, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.00509 8200.00 8000.00 0.00 -0.00435 8400.00 8000.00 0.00 -0.00345 8600.00 8000.00 0.00 -0.00252 8800.00 8000.00 0.00 -0.00163 9000.00 8000.00 0.00 -0.00078 9200.00 8000.00 0.00 0.00010 9400.00 8000.00 0.00 0.00105 9600.00 8000.00 0.00 0.00211 9800.00 8000.00 0.00 0.00325 10000.00 8000.00 0.00 0.00442 10200.00 8000.00 0.00 0.00561 10400.00 8000.00 0.00 0.00684 10600.00 8000.00 0.00 0.00817 10800.00 8000.00 0.00 0.00966 11000.00 8000.00 0.00 0.01132 11200.00 8000.00 0.00 0.01316 11400.00 8000.00 0.00 0.01513 11600.00 8000.00 0.00 0.01720 11800.00 8000.00 0.00 0.01937 12000.00 8000.00 0.00 0.02166 12200.00 8000.00 0.00 0.02412 12400.00 8000.00 0.00 0.02680 12600.00 8000.00 0.00 0.02970 12800.00 8000.00 0.00 0.03278 13000.00 8000.00 0.00 0.03590 13200.00 8000.00 0.00 0.03899 13400.00 8000.00 0.00 0.04197 13600.00 8000.00 0.00 0.04489 13800.00 8000.00 0.00 0.04791 14000.00 8000.00 0.00 0.05113 14200.00 8000.00 0.00 0.05461 14400.00 8000.00 0.00 0.05827 14600.00 8000.00 0.00 0.06198 14800.00 8000.00 0.00 0.06555 15000.00 8000.00 0.00 0.06898 15200.00 8000.00 0.00 0.07235 15400.00 8000.00 0.00 0.07583 15600.00 8000.00 0.00 0.07959 15800.00 8000.00 0.00 0.08366 16000.00 8000.00 0.00 0.08789 16200.00 8000.00 0.00 0.09199 16400.00 8000.00 0.00 0.09569 16600.00 8000.00 0.00 0.09868 16800.00 8000.00 0.00 0.10086 17000.00 8000.00 0.00 0.10234 17200.00 8000.00 0.00 0.10326 17400.00 8000.00 0.00 0.10383 17600.00 8000.00 0.00 0.10425 17800.00 8000.00 0.00 0.10455 18000.00 8000.00 0.00 0.10467 17200.00 8200.00 0.00 0.10508 17400.00 8200.00 0.00 0.10565 17600.00 8200.00 0.00 0.10607 17800.00 8200.00 0.00 0.10636 18000.00 8200.00 0.00 0.10645 17200.00 8400.00 0.00 0.10697 17400.00 8400.00 0.00 0.10753 17600.00 8400.00 0.00 0.10792 17800.00 8400.00 0.00 0.10817 18000.00 8400.00 0.00 0.10822 17200.00 8600.00 0.00 0.10906 17400.00 8600.00 0.00 0.10959 17600.00 8600.00 0.00 0.10995 17800.00 8600.00 0.00 0.11015 17000.00 8800.00 0.00 0.11044 17200.00 8800.00 0.00 0.11130 17400.00 8800.00 0.00 0.11179 17600.00 8800.00 0.00 0.11210 17800.00 8800.00 0.00 0.11226 17000.00 9000.00 0.00 0.11268 17200.00 9000.00 0.00 0.11351 17400.00 9000.00 0.00 0.11397 17600.00 9000.00 0.00 0.11425 17800.00 9000.00 0.00 0.11437 17000.00 9200.00 0.00 0.11483 17200.00 9200.00 0.00 0.11564 17400.00 9200.00 0.00 0.11607 17600.00 9200.00 0.00 0.11632 17800.00 9200.00 0.00 0.11641 17000.00 9400.00 0.00 0.11687 17200.00 9400.00 0.00 0.11767 17400.00 9400.00 0.00 0.11808 17600.00 9400.00 0.00 0.11831 17800.00 9400.00 0.00 0.11837 17000.00 9600.00 0.00 0.11889 17200.00 9600.00 0.00 0.11968 17400.00 9600.00 0.00 0.12008 17600.00 9600.00 0.00 0.12029 17800.00 9600.00 0.00 0.12033 17000.00 9800.00 0.00 0.12117 17200.00 9800.00 0.00 0.12196 17400.00 9800.00 0.00 0.12235 17600.00 9800.00 0.00 0.12254 17800.00 9800.00 0.00 0.12256 17000.00 10000.00 0.00 0.12384 17200.00 10000.00 0.00 0.12463 17400.00 10000.00 0.00 0.12501 17600.00 10000.00 0.00 0.12517 16800.00 10200.00 0.00 0.12530 17000.00 10200.00 0.00 0.12672 17200.00 10200.00 0.00 0.12751 17400.00 10200.00 0.00 0.12788 17600.00 10200.00 0.00 0.12803 16800.00 10400.00 0.00 0.12822 17000.00 10400.00 0.00 0.12966 17200.00 10400.00 0.00 0.13047 17400.00 10400.00 0.00 0.13084 17600.00 10400.00 0.00 0.13096 16800.00 10600.00 0.00 0.13107 17000.00 10600.00 0.00 0.13255 17200.00 10600.00 0.00 0.13337 17400.00 10600.00 0.00 0.13372 17600.00 10600.00 0.00 0.13381 17000.00 10800.00 0.00 0.13527 17200.00 10800.00 0.00 0.13610 17400.00 10800.00 0.00 0.13643 17600.00 10800.00 0.00 0.13646 16800.00 11000.00 0.00 0.13649 17000.00 11000.00 0.00 0.13803 17200.00 11000.00 0.00 0.13884 17400.00 11000.00 0.00 0.13912 16800.00 11200.00 0.00 0.13953 17000.00 11200.00 0.00 0.14107 17200.00 11200.00 0.00 0.14185 17400.00 11200.00 0.00 0.14205 16800.00 11400.00 0.00 0.14283 17000.00 11400.00 0.00 0.14435 17200.00 11400.00 0.00 0.14507 17400.00 11400.00 0.00 0.14519 16800.00 11600.00 0.00 0.14628 17000.00 11600.00 0.00 0.14776 17200.00 11600.00 0.00 0.14840 17400.00 11600.00 0.00 0.14842 16800.00 11800.00 0.00 0.14978 17000.00 11800.00 0.00 0.15120 17200.00 11800.00 0.00 0.15174 16800.00 12000.00 0.00 0.15317 17000.00 12000.00 0.00 0.15452 17200.00 12000.00 0.00 0.15496 16800.00 12200.00 0.00 0.15651 17000.00 12200.00 0.00 0.15778 17200.00 12200.00 0.00 0.15812 16800.00 12400.00 0.00 0.16015 17000.00 12400.00 0.00 0.16134 17200.00 12400.00 0.00 0.16159 16600.00 12600.00 0.00 0.16197 16800.00 12600.00 0.00 0.16418 17000.00 12600.00 0.00 0.16531 17200.00 12600.00 0.00 0.16548 16600.00 12800.00 0.00 0.16630 16800.00 12800.00 0.00 0.16848 17000.00 12800.00 0.00 0.16956 17200.00 12800.00 0.00 0.16965 16600.00 13000.00 0.00 0.17083 16800.00 13000.00 0.00 0.17299 17000.00 13000.00 0.00 0.17401 17200.00 13000.00 0.00 0.17403 16600.00 13200.00 0.00 0.17541 16800.00 13200.00 0.00 0.17755 17000.00 13200.00 0.00 0.17852 16600.00 13400.00 0.00 0.17993 16800.00 13400.00 0.00 0.18203 17000.00 13400.00 0.00 0.18293 16600.00 13600.00 0.00 0.18463 16800.00 13600.00 0.00 0.18667 17000.00 13600.00 0.00 0.18745 16600.00 13800.00 0.00 0.18971 16800.00 13800.00 0.00 0.19165 17000.00 13800.00 0.00 0.19228 16600.00 14000.00 0.00 0.19502 16800.00 14000.00 0.00 0.19682 17000.00 14000.00 0.00 0.19727 16400.00 14200.00 0.00 0.19731 16600.00 14200.00 0.00 0.20039 16800.00 14200.00 0.00 0.20202 17000.00 14200.00 0.00 0.20225 16400.00 14400.00 0.00 0.20271 16600.00 14400.00 0.00 0.20566 16800.00 14400.00 0.00 0.20710 16400.00 14600.00 0.00 0.20778 16600.00 14600.00 0.00 0.21058 16800.00 14600.00 0.00 0.21181 16400.00 14800.00 0.00 0.21268 16600.00 14800.00 0.00 0.21531 16800.00 14800.00 0.00 0.21630 16400.00 15000.00 0.00 0.21782 16600.00 15000.00 0.00 0.22022 16800.00 15000.00 0.00 0.22092 16400.00 15200.00 0.00 0.22317 16600.00 15200.00 0.00 0.22528 16800.00 15200.00 0.00 0.22564 16400.00 15400.00 0.00 0.22855 16600.00 15400.00 0.00 0.23030 16200.00 15600.00 0.00 0.23089 16400.00 15600.00 0.00 0.23378 16600.00 15600.00 0.00 0.23508 16200.00 15800.00 0.00 0.23582 16400.00 15800.00 0.00 0.23823 16600.00 15800.00 0.00 0.23902 16200.00 16000.00 0.00 0.23939 16400.00 16000.00 0.00 0.24134 16600.00 16000.00 0.00 0.24161 16200.00 16200.00 0.00 0.24172 16400.00 16200.00 0.00 0.24322 16400.00 16400.00 0.00 0.24370 16200.00 16600.00 25.00 0.24397 16200.00 16600.00 30.00 0.24429 16200.00 16600.00 35.00 0.24455 16200.00 16600.00 40.00 0.24474 16200.00 16600.00 45.00 0.24486 16200.00 16600.00 50.00 0.24491 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16200.00 16608.56 50.00 0.24491 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/272.ctf NX, NY, NZ: 360 360 1 MODE : real