CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/276.mrc Output diagnostic file name Micrographs/276.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/276.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2404.000 9120.000 Mean, RMS : 4452.512 224.7301 TITLE 1: emi2mrc: 276.emi Sun Jan 19 23:00:30 2014 TITLE 2: kV 300, Mag 78000x, Def 21330, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -471.056, Y 540.991, Z -22.843, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01791 8200.00 8000.00 0.00 -0.01757 8400.00 8000.00 0.00 -0.01713 8600.00 8000.00 0.00 -0.01668 8800.00 8000.00 0.00 -0.01629 9000.00 8000.00 0.00 -0.01591 9200.00 8000.00 0.00 -0.01547 9400.00 8000.00 0.00 -0.01498 9600.00 8000.00 0.00 -0.01442 9800.00 8000.00 0.00 -0.01381 10000.00 8000.00 0.00 -0.01321 10200.00 8000.00 0.00 -0.01259 10400.00 8000.00 0.00 -0.01188 10600.00 8000.00 0.00 -0.01105 10800.00 8000.00 0.00 -0.01005 11000.00 8000.00 0.00 -0.00891 11200.00 8000.00 0.00 -0.00768 11400.00 8000.00 0.00 -0.00645 11600.00 8000.00 0.00 -0.00528 11800.00 8000.00 0.00 -0.00422 12000.00 8000.00 0.00 -0.00323 12200.00 8000.00 0.00 -0.00225 12400.00 8000.00 0.00 -0.00119 12600.00 8000.00 0.00 0.00004 12800.00 8000.00 0.00 0.00147 13000.00 8000.00 0.00 0.00306 13200.00 8000.00 0.00 0.00476 13400.00 8000.00 0.00 0.00648 13600.00 8000.00 0.00 0.00823 13800.00 8000.00 0.00 0.01005 14000.00 8000.00 0.00 0.01204 14200.00 8000.00 0.00 0.01422 14400.00 8000.00 0.00 0.01661 14600.00 8000.00 0.00 0.01913 14800.00 8000.00 0.00 0.02172 15000.00 8000.00 0.00 0.02436 15200.00 8000.00 0.00 0.02705 15400.00 8000.00 0.00 0.02982 15600.00 8000.00 0.00 0.03266 15800.00 8000.00 0.00 0.03553 16000.00 8000.00 0.00 0.03838 16200.00 8000.00 0.00 0.04124 16400.00 8000.00 0.00 0.04421 16600.00 8000.00 0.00 0.04733 16800.00 8000.00 0.00 0.05063 17000.00 8000.00 0.00 0.05406 17200.00 8000.00 0.00 0.05752 17400.00 8000.00 0.00 0.06094 17600.00 8000.00 0.00 0.06438 17800.00 8000.00 0.00 0.06791 18000.00 8000.00 0.00 0.07160 18200.00 8000.00 0.00 0.07541 18400.00 8000.00 0.00 0.07916 18600.00 8000.00 0.00 0.08256 18800.00 8000.00 0.00 0.08538 19000.00 8000.00 0.00 0.08748 19200.00 8000.00 0.00 0.08885 19400.00 8000.00 0.00 0.08962 19600.00 8000.00 0.00 0.08998 19800.00 8000.00 0.00 0.09011 20000.00 8000.00 0.00 0.09015 19400.00 8200.00 0.00 0.09084 19600.00 8200.00 0.00 0.09119 19800.00 8200.00 0.00 0.09130 20000.00 8200.00 0.00 0.09131 19200.00 8400.00 0.00 0.09135 19400.00 8400.00 0.00 0.09209 19600.00 8400.00 0.00 0.09242 19800.00 8400.00 0.00 0.09251 19200.00 8600.00 0.00 0.09280 19400.00 8600.00 0.00 0.09352 19600.00 8600.00 0.00 0.09383 19800.00 8600.00 0.00 0.09390 19200.00 8800.00 0.00 0.09439 19400.00 8800.00 0.00 0.09510 19600.00 8800.00 0.00 0.09539 19800.00 8800.00 0.00 0.09544 19200.00 9000.00 0.00 0.09600 19400.00 9000.00 0.00 0.09669 19600.00 9000.00 0.00 0.09697 19800.00 9000.00 0.00 0.09700 19200.00 9200.00 0.00 0.09759 19400.00 9200.00 0.00 0.09827 19600.00 9200.00 0.00 0.09854 19800.00 9200.00 0.00 0.09855 19200.00 9400.00 0.00 0.09915 19400.00 9400.00 0.00 0.09983 19600.00 9400.00 0.00 0.10008 19800.00 9400.00 0.00 0.10009 19200.00 9600.00 0.00 0.10075 19400.00 9600.00 0.00 0.10142 19600.00 9600.00 0.00 0.10165 19200.00 9800.00 0.00 0.10255 19400.00 9800.00 0.00 0.10319 19600.00 9800.00 0.00 0.10340 19200.00 10000.00 0.00 0.10457 19400.00 10000.00 0.00 0.10517 19600.00 10000.00 0.00 0.10534 19000.00 10200.00 0.00 0.10541 19200.00 10200.00 0.00 0.10662 19400.00 10200.00 0.00 0.10716 19600.00 10200.00 0.00 0.10727 19000.00 10400.00 0.00 0.10740 19200.00 10400.00 0.00 0.10855 19400.00 10400.00 0.00 0.10901 19600.00 10400.00 0.00 0.10906 19000.00 10600.00 0.00 0.10918 19200.00 10600.00 0.00 0.11026 19400.00 10600.00 0.00 0.11065 19000.00 10800.00 0.00 0.11076 19200.00 10800.00 0.00 0.11177 19400.00 10800.00 0.00 0.11211 19000.00 11000.00 0.00 0.11236 19200.00 11000.00 0.00 0.11332 19400.00 11000.00 0.00 0.11363 19000.00 11200.00 0.00 0.11419 19200.00 11200.00 0.00 0.11512 19400.00 11200.00 0.00 0.11541 19000.00 11400.00 0.00 0.11620 19200.00 11400.00 0.00 0.11711 19400.00 11400.00 0.00 0.11739 19000.00 11600.00 0.00 0.11826 19200.00 11600.00 0.00 0.11915 19400.00 11600.00 0.00 0.11943 19000.00 11800.00 0.00 0.12026 19200.00 11800.00 0.00 0.12113 19400.00 11800.00 0.00 0.12141 19000.00 12000.00 0.00 0.12209 19200.00 12000.00 0.00 0.12296 19400.00 12000.00 0.00 0.12323 19000.00 12200.00 0.00 0.12389 19200.00 12200.00 0.00 0.12476 19400.00 12200.00 0.00 0.12503 19000.00 12400.00 0.00 0.12595 19200.00 12400.00 0.00 0.12682 19400.00 12400.00 0.00 0.12709 19000.00 12600.00 0.00 0.12834 19200.00 12600.00 0.00 0.12921 19400.00 12600.00 0.00 0.12948 19000.00 12800.00 0.00 0.13094 19200.00 12800.00 0.00 0.13181 19400.00 12800.00 0.00 0.13207 19000.00 13000.00 0.00 0.13363 19200.00 13000.00 0.00 0.13449 19400.00 13000.00 0.00 0.13474 19000.00 13200.00 0.00 0.13625 19200.00 13200.00 0.00 0.13710 19400.00 13200.00 0.00 0.13734 19000.00 13400.00 0.00 0.13874 19200.00 13400.00 0.00 0.13958 19400.00 13400.00 0.00 0.13980 19000.00 13600.00 0.00 0.14131 19200.00 13600.00 0.00 0.14214 19400.00 13600.00 0.00 0.14234 18800.00 13800.00 0.00 0.14245 19000.00 13800.00 0.00 0.14414 19200.00 13800.00 0.00 0.14496 19400.00 13800.00 0.00 0.14513 18800.00 14000.00 0.00 0.14549 19000.00 14000.00 0.00 0.14718 19200.00 14000.00 0.00 0.14798 19400.00 14000.00 0.00 0.14812 18800.00 14200.00 0.00 0.14870 19000.00 14200.00 0.00 0.15040 19200.00 14200.00 0.00 0.15120 19400.00 14200.00 0.00 0.15130 18800.00 14400.00 0.00 0.15207 19000.00 14400.00 0.00 0.15377 19200.00 14400.00 0.00 0.15455 19400.00 14400.00 0.00 0.15461 18800.00 14600.00 0.00 0.15548 19000.00 14600.00 0.00 0.15718 19200.00 14600.00 0.00 0.15792 18800.00 14800.00 0.00 0.15904 19000.00 14800.00 0.00 0.16070 19200.00 14800.00 0.00 0.16140 18800.00 15000.00 0.00 0.16292 19000.00 15000.00 0.00 0.16454 19200.00 15000.00 0.00 0.16516 18800.00 15200.00 0.00 0.16700 19000.00 15200.00 0.00 0.16856 19200.00 15200.00 0.00 0.16909 18800.00 15400.00 0.00 0.17106 19000.00 15400.00 0.00 0.17255 19200.00 15400.00 0.00 0.17298 18800.00 15600.00 0.00 0.17503 19000.00 15600.00 0.00 0.17644 19200.00 15600.00 0.00 0.17679 18800.00 15800.00 0.00 0.17880 19000.00 15800.00 0.00 0.18017 19200.00 15800.00 0.00 0.18043 18800.00 16000.00 0.00 0.18250 19000.00 16000.00 0.00 0.18381 19200.00 16000.00 0.00 0.18399 18800.00 16200.00 0.00 0.18647 19000.00 16200.00 0.00 0.18772 19200.00 16200.00 0.00 0.18780 18600.00 16400.00 0.00 0.18838 18800.00 16400.00 0.00 0.19082 19000.00 16400.00 0.00 0.19198 18600.00 16600.00 0.00 0.19293 18800.00 16600.00 0.00 0.19531 19000.00 16600.00 0.00 0.19634 18600.00 16800.00 0.00 0.19746 18800.00 16800.00 0.00 0.19974 19000.00 16800.00 0.00 0.20062 18600.00 17000.00 0.00 0.20181 18800.00 17000.00 0.00 0.20397 19000.00 17000.00 0.00 0.20466 18600.00 17200.00 0.00 0.20594 18800.00 17200.00 0.00 0.20795 19000.00 17200.00 0.00 0.20842 18600.00 17400.00 0.00 0.21034 18800.00 17400.00 0.00 0.21213 19000.00 17400.00 0.00 0.21231 18600.00 17600.00 0.00 0.21538 18800.00 17600.00 0.00 0.21685 18400.00 17800.00 0.00 0.21776 18600.00 17800.00 0.00 0.22080 18800.00 17800.00 0.00 0.22181 18400.00 18000.00 0.00 0.22354 18600.00 18000.00 0.00 0.22606 18800.00 18000.00 0.00 0.22648 18400.00 18200.00 0.00 0.22863 18600.00 18200.00 0.00 0.23045 18400.00 18400.00 0.00 0.23192 18600.00 18400.00 0.00 0.23291 18600.00 18600.00 0.00 0.23308 18400.00 18600.00 30.00 0.23309 18400.00 18600.00 35.00 0.23312 18400.00 18600.00 40.00 0.23315 18400.00 18600.00 45.00 0.23316 18400.00 18600.00 50.00 0.23317 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18493.73 18592.62 48.48 0.23343 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/276.ctf NX, NY, NZ: 360 360 1 MODE : real