CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/277.mrc Output diagnostic file name Micrographs/277.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/277.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2344.000 8120.000 Mean, RMS : 4458.354 225.2912 TITLE 1: emi2mrc: 277.emi Sun Jan 19 23:03:00 2014 TITLE 2: kV 300, Mag 78000x, Def 24120, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -478.234, Y 549.199, Z -22.843, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01681 8600.00 8000.00 0.00 -0.01678 8800.00 8000.00 0.00 -0.01673 11400.00 8000.00 0.00 -0.01634 11600.00 8000.00 0.00 -0.01557 11800.00 8000.00 0.00 -0.01473 12000.00 8000.00 0.00 -0.01391 12200.00 8000.00 0.00 -0.01315 12400.00 8000.00 0.00 -0.01245 12600.00 8000.00 0.00 -0.01179 12800.00 8000.00 0.00 -0.01108 13000.00 8000.00 0.00 -0.01025 13200.00 8000.00 0.00 -0.00927 13400.00 8000.00 0.00 -0.00815 13600.00 8000.00 0.00 -0.00697 13800.00 8000.00 0.00 -0.00582 14000.00 8000.00 0.00 -0.00473 14200.00 8000.00 0.00 -0.00370 14400.00 8000.00 0.00 -0.00266 14600.00 8000.00 0.00 -0.00152 14800.00 8000.00 0.00 -0.00022 15000.00 8000.00 0.00 0.00128 15200.00 8000.00 0.00 0.00298 15400.00 8000.00 0.00 0.00487 15600.00 8000.00 0.00 0.00696 15800.00 8000.00 0.00 0.00925 16000.00 8000.00 0.00 0.01169 16200.00 8000.00 0.00 0.01421 16400.00 8000.00 0.00 0.01672 16600.00 8000.00 0.00 0.01915 16800.00 8000.00 0.00 0.02145 17000.00 8000.00 0.00 0.02368 17200.00 8000.00 0.00 0.02588 17400.00 8000.00 0.00 0.02809 17600.00 8000.00 0.00 0.03033 17800.00 8000.00 0.00 0.03258 18000.00 8000.00 0.00 0.03481 18200.00 8000.00 0.00 0.03707 18400.00 8000.00 0.00 0.03940 18600.00 8000.00 0.00 0.04188 18800.00 8000.00 0.00 0.04452 19000.00 8000.00 0.00 0.04731 19200.00 8000.00 0.00 0.05018 19400.00 8000.00 0.00 0.05315 19600.00 8000.00 0.00 0.05631 19800.00 8000.00 0.00 0.05975 20000.00 8000.00 0.00 0.06355 20200.00 8000.00 0.00 0.06758 20400.00 8000.00 0.00 0.07151 20600.00 8000.00 0.00 0.07490 20800.00 8000.00 0.00 0.07742 21000.00 8000.00 0.00 0.07889 21200.00 8000.00 0.00 0.07949 21400.00 8000.00 0.00 0.07953 21200.00 8200.00 0.00 0.08007 21400.00 8200.00 0.00 0.08008 21000.00 8400.00 0.00 0.08013 21200.00 8400.00 0.00 0.08066 21000.00 8600.00 0.00 0.08092 21200.00 8600.00 0.00 0.08142 21000.00 8800.00 0.00 0.08183 21200.00 8800.00 0.00 0.08232 21000.00 9000.00 0.00 0.08275 21200.00 9000.00 0.00 0.08324 21000.00 9200.00 0.00 0.08364 21200.00 9200.00 0.00 0.08413 21000.00 9400.00 0.00 0.08446 21200.00 9400.00 0.00 0.08496 21000.00 9600.00 0.00 0.08526 21200.00 9600.00 0.00 0.08577 21000.00 9800.00 0.00 0.08622 21200.00 9800.00 0.00 0.08672 21000.00 10000.00 0.00 0.08737 21200.00 10000.00 0.00 0.08786 21000.00 10200.00 0.00 0.08858 21200.00 10200.00 0.00 0.08906 21000.00 10400.00 0.00 0.08977 21200.00 10400.00 0.00 0.09022 21000.00 10600.00 0.00 0.09089 21200.00 10600.00 0.00 0.09133 21000.00 10800.00 0.00 0.09195 21200.00 10800.00 0.00 0.09240 21000.00 11000.00 0.00 0.09310 21200.00 11000.00 0.00 0.09358 21000.00 11200.00 0.00 0.09451 21200.00 11200.00 0.00 0.09501 21000.00 11400.00 0.00 0.09611 21200.00 11400.00 0.00 0.09663 21000.00 11600.00 0.00 0.09783 21200.00 11600.00 0.00 0.09836 21000.00 11800.00 0.00 0.09962 21200.00 11800.00 0.00 0.10014 21000.00 12000.00 0.00 0.10143 21200.00 12000.00 0.00 0.10193 21000.00 12200.00 0.00 0.10329 21200.00 12200.00 0.00 0.10375 20800.00 12400.00 0.00 0.10381 21000.00 12400.00 0.00 0.10530 21200.00 12400.00 0.00 0.10571 20800.00 12600.00 0.00 0.10595 21000.00 12600.00 0.00 0.10740 21200.00 12600.00 0.00 0.10773 20800.00 12800.00 0.00 0.10805 21000.00 12800.00 0.00 0.10945 21200.00 12800.00 0.00 0.10971 20800.00 13000.00 0.00 0.11004 21000.00 13000.00 0.00 0.11138 21200.00 13000.00 0.00 0.11158 20800.00 13200.00 0.00 0.11189 21000.00 13200.00 0.00 0.11319 21200.00 13200.00 0.00 0.11333 20800.00 13400.00 0.00 0.11367 21000.00 13400.00 0.00 0.11493 21200.00 13400.00 0.00 0.11500 20800.00 13600.00 0.00 0.11558 21000.00 13600.00 0.00 0.11679 21200.00 13600.00 0.00 0.11681 20800.00 13800.00 0.00 0.11772 21000.00 13800.00 0.00 0.11887 20800.00 14000.00 0.00 0.11999 21000.00 14000.00 0.00 0.12109 20800.00 14200.00 0.00 0.12234 21000.00 14200.00 0.00 0.12340 20800.00 14400.00 0.00 0.12476 21000.00 14400.00 0.00 0.12576 20800.00 14600.00 0.00 0.12717 21000.00 14600.00 0.00 0.12813 20800.00 14800.00 0.00 0.12967 21000.00 14800.00 0.00 0.13057 20800.00 15000.00 0.00 0.13233 21000.00 15000.00 0.00 0.13318 20800.00 15200.00 0.00 0.13504 21000.00 15200.00 0.00 0.13587 20800.00 15400.00 0.00 0.13769 21000.00 15400.00 0.00 0.13853 20800.00 15600.00 0.00 0.14031 21000.00 15600.00 0.00 0.14118 20800.00 15800.00 0.00 0.14292 21000.00 15800.00 0.00 0.14384 20800.00 16000.00 0.00 0.14564 21000.00 16000.00 0.00 0.14662 20800.00 16200.00 0.00 0.14875 21000.00 16200.00 0.00 0.14980 20800.00 16400.00 0.00 0.15225 21000.00 16400.00 0.00 0.15336 20800.00 16600.00 0.00 0.15594 21000.00 16600.00 0.00 0.15709 20800.00 16800.00 0.00 0.15974 21000.00 16800.00 0.00 0.16090 20800.00 17000.00 0.00 0.16359 21000.00 17000.00 0.00 0.16471 20800.00 17200.00 0.00 0.16740 21000.00 17200.00 0.00 0.16845 20600.00 17400.00 0.00 0.16859 20800.00 17400.00 0.00 0.17135 21000.00 17400.00 0.00 0.17227 20600.00 17600.00 0.00 0.17282 20800.00 17600.00 0.00 0.17550 21000.00 17600.00 0.00 0.17627 20600.00 17800.00 0.00 0.17708 20800.00 17800.00 0.00 0.17966 21000.00 17800.00 0.00 0.18026 20600.00 18000.00 0.00 0.18123 20800.00 18000.00 0.00 0.18370 21000.00 18000.00 0.00 0.18411 20600.00 18200.00 0.00 0.18530 20800.00 18200.00 0.00 0.18765 21000.00 18200.00 0.00 0.18787 20600.00 18400.00 0.00 0.18933 20800.00 18400.00 0.00 0.19155 21000.00 18400.00 0.00 0.19159 20600.00 18600.00 0.00 0.19354 20800.00 18600.00 0.00 0.19564 20600.00 18800.00 0.00 0.19823 20800.00 18800.00 0.00 0.20019 20600.00 19000.00 0.00 0.20330 20800.00 19000.00 0.00 0.20513 20600.00 19200.00 0.00 0.20857 20800.00 19200.00 0.00 0.21025 20600.00 19400.00 0.00 0.21408 20800.00 19400.00 0.00 0.21556 20400.00 19600.00 0.00 0.21603 20600.00 19600.00 0.00 0.21979 20800.00 19600.00 0.00 0.22098 20400.00 19800.00 0.00 0.22211 20600.00 19800.00 0.00 0.22565 20800.00 19800.00 0.00 0.22643 20400.00 20000.00 0.00 0.22855 20600.00 20000.00 0.00 0.23166 20800.00 20000.00 0.00 0.23185 20400.00 20200.00 0.00 0.23468 20600.00 20200.00 0.00 0.23712 20400.00 20400.00 0.00 0.23901 20600.00 20400.00 0.00 0.24053 20600.00 20600.00 0.00 0.24086 20400.00 20600.00 20.00 0.24089 20400.00 20600.00 25.00 0.24100 20400.00 20600.00 30.00 0.24109 20400.00 20600.00 35.00 0.24116 20400.00 20600.00 40.00 0.24121 20400.00 20600.00 45.00 0.24123 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20471.29 20618.06 48.37 0.24150 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/277.ctf NX, NY, NZ: 360 360 1 MODE : real