CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/285.mrc Output diagnostic file name Micrographs/285.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/285.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2228.000 8648.000 Mean, RMS : 4425.168 223.4503 TITLE 1: emi2mrc: 285.emi Sun Jan 19 23:09:38 2014 TITLE 2: kV 300, Mag 78000x, Def 23460, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -491.210, Y 532.625, Z -23.053, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01269 8200.00 8000.00 0.00 -0.01259 8400.00 8000.00 0.00 -0.01244 8600.00 8000.00 0.00 -0.01228 8800.00 8000.00 0.00 -0.01210 9000.00 8000.00 0.00 -0.01184 9200.00 8000.00 0.00 -0.01145 9400.00 8000.00 0.00 -0.01092 9600.00 8000.00 0.00 -0.01030 9800.00 8000.00 0.00 -0.00968 10000.00 8000.00 0.00 -0.00916 10200.00 8000.00 0.00 -0.00873 10400.00 8000.00 0.00 -0.00834 10600.00 8000.00 0.00 -0.00793 10800.00 8000.00 0.00 -0.00742 11000.00 8000.00 0.00 -0.00677 11200.00 8000.00 0.00 -0.00598 11400.00 8000.00 0.00 -0.00504 11600.00 8000.00 0.00 -0.00395 11800.00 8000.00 0.00 -0.00272 12000.00 8000.00 0.00 -0.00140 12200.00 8000.00 0.00 -0.00006 12400.00 8000.00 0.00 0.00125 12600.00 8000.00 0.00 0.00247 12800.00 8000.00 0.00 0.00363 13000.00 8000.00 0.00 0.00476 13200.00 8000.00 0.00 0.00592 13400.00 8000.00 0.00 0.00713 13600.00 8000.00 0.00 0.00842 13800.00 8000.00 0.00 0.00980 14000.00 8000.00 0.00 0.01132 14200.00 8000.00 0.00 0.01299 14400.00 8000.00 0.00 0.01484 14600.00 8000.00 0.00 0.01686 14800.00 8000.00 0.00 0.01901 15000.00 8000.00 0.00 0.02124 15200.00 8000.00 0.00 0.02354 15400.00 8000.00 0.00 0.02590 15600.00 8000.00 0.00 0.02831 15800.00 8000.00 0.00 0.03077 16000.00 8000.00 0.00 0.03325 16200.00 8000.00 0.00 0.03577 16400.00 8000.00 0.00 0.03838 16600.00 8000.00 0.00 0.04111 16800.00 8000.00 0.00 0.04404 17000.00 8000.00 0.00 0.04716 17200.00 8000.00 0.00 0.05041 17400.00 8000.00 0.00 0.05372 17600.00 8000.00 0.00 0.05711 17800.00 8000.00 0.00 0.06065 18000.00 8000.00 0.00 0.06444 18200.00 8000.00 0.00 0.06854 18400.00 8000.00 0.00 0.07288 18600.00 8000.00 0.00 0.07720 18800.00 8000.00 0.00 0.08115 19000.00 8000.00 0.00 0.08436 19200.00 8000.00 0.00 0.08660 19400.00 8000.00 0.00 0.08788 19600.00 8000.00 0.00 0.08840 19800.00 8000.00 0.00 0.08846 19400.00 8200.00 0.00 0.08893 19600.00 8200.00 0.00 0.08945 19800.00 8200.00 0.00 0.08951 19400.00 8400.00 0.00 0.08995 19600.00 8400.00 0.00 0.09046 19800.00 8400.00 0.00 0.09051 19400.00 8600.00 0.00 0.09111 19600.00 8600.00 0.00 0.09162 19800.00 8600.00 0.00 0.09166 19400.00 8800.00 0.00 0.09244 19600.00 8800.00 0.00 0.09294 19800.00 8800.00 0.00 0.09298 19400.00 9000.00 0.00 0.09387 19600.00 9000.00 0.00 0.09437 19800.00 9000.00 0.00 0.09440 19400.00 9200.00 0.00 0.09537 19600.00 9200.00 0.00 0.09587 19800.00 9200.00 0.00 0.09590 19400.00 9400.00 0.00 0.09687 19600.00 9400.00 0.00 0.09738 19800.00 9400.00 0.00 0.09741 19400.00 9600.00 0.00 0.09834 19600.00 9600.00 0.00 0.09886 19800.00 9600.00 0.00 0.09891 19400.00 9800.00 0.00 0.09990 19600.00 9800.00 0.00 0.10044 19800.00 9800.00 0.00 0.10050 19400.00 10000.00 0.00 0.10161 19600.00 10000.00 0.00 0.10217 19800.00 10000.00 0.00 0.10223 19400.00 10200.00 0.00 0.10343 19600.00 10200.00 0.00 0.10400 19800.00 10200.00 0.00 0.10405 19400.00 10400.00 0.00 0.10534 19600.00 10400.00 0.00 0.10591 19800.00 10400.00 0.00 0.10593 19200.00 10600.00 0.00 0.10598 19400.00 10600.00 0.00 0.10732 19600.00 10600.00 0.00 0.10787 19200.00 10800.00 0.00 0.10800 19400.00 10800.00 0.00 0.10933 19600.00 10800.00 0.00 0.10986 19200.00 11000.00 0.00 0.11012 19400.00 11000.00 0.00 0.11145 19600.00 11000.00 0.00 0.11194 19200.00 11200.00 0.00 0.11241 19400.00 11200.00 0.00 0.11371 19600.00 11200.00 0.00 0.11417 19200.00 11400.00 0.00 0.11474 19400.00 11400.00 0.00 0.11601 19600.00 11400.00 0.00 0.11643 19200.00 11600.00 0.00 0.11700 19400.00 11600.00 0.00 0.11824 19600.00 11600.00 0.00 0.11861 19200.00 11800.00 0.00 0.11918 19400.00 11800.00 0.00 0.12039 19600.00 11800.00 0.00 0.12072 19200.00 12000.00 0.00 0.12127 19400.00 12000.00 0.00 0.12245 19600.00 12000.00 0.00 0.12274 19200.00 12200.00 0.00 0.12338 19400.00 12200.00 0.00 0.12454 19600.00 12200.00 0.00 0.12481 19200.00 12400.00 0.00 0.12575 19400.00 12400.00 0.00 0.12690 19600.00 12400.00 0.00 0.12714 19200.00 12600.00 0.00 0.12839 19400.00 12600.00 0.00 0.12952 19600.00 12600.00 0.00 0.12972 19200.00 12800.00 0.00 0.13117 19400.00 12800.00 0.00 0.13227 19600.00 12800.00 0.00 0.13244 19200.00 13000.00 0.00 0.13402 19400.00 13000.00 0.00 0.13509 19600.00 13000.00 0.00 0.13522 19200.00 13200.00 0.00 0.13684 19400.00 13200.00 0.00 0.13788 19600.00 13200.00 0.00 0.13798 19200.00 13400.00 0.00 0.13961 19400.00 13400.00 0.00 0.14061 19600.00 13400.00 0.00 0.14066 19200.00 13600.00 0.00 0.14248 19400.00 13600.00 0.00 0.14344 19000.00 13800.00 0.00 0.14346 19200.00 13800.00 0.00 0.14554 19400.00 13800.00 0.00 0.14644 19000.00 14000.00 0.00 0.14662 19200.00 14000.00 0.00 0.14866 19400.00 14000.00 0.00 0.14952 19000.00 14200.00 0.00 0.14978 19200.00 14200.00 0.00 0.15180 19400.00 14200.00 0.00 0.15263 19000.00 14400.00 0.00 0.15296 19200.00 14400.00 0.00 0.15496 19400.00 14400.00 0.00 0.15576 19000.00 14600.00 0.00 0.15610 19200.00 14600.00 0.00 0.15811 19400.00 14600.00 0.00 0.15890 19000.00 14800.00 0.00 0.15939 19200.00 14800.00 0.00 0.16141 19400.00 14800.00 0.00 0.16220 19000.00 15000.00 0.00 0.16309 19200.00 15000.00 0.00 0.16513 19400.00 15000.00 0.00 0.16592 19000.00 15200.00 0.00 0.16714 19200.00 15200.00 0.00 0.16920 19400.00 15200.00 0.00 0.16996 19000.00 15400.00 0.00 0.17135 19200.00 15400.00 0.00 0.17341 19400.00 15400.00 0.00 0.17415 19000.00 15600.00 0.00 0.17563 19200.00 15600.00 0.00 0.17768 19400.00 15600.00 0.00 0.17837 19000.00 15800.00 0.00 0.17982 19200.00 15800.00 0.00 0.18186 19400.00 15800.00 0.00 0.18248 19000.00 16000.00 0.00 0.18392 19200.00 16000.00 0.00 0.18592 19400.00 16000.00 0.00 0.18646 19000.00 16200.00 0.00 0.18822 19200.00 16200.00 0.00 0.19015 19400.00 16200.00 0.00 0.19057 19000.00 16400.00 0.00 0.19280 19200.00 16400.00 0.00 0.19465 19400.00 16400.00 0.00 0.19492 19000.00 16600.00 0.00 0.19751 19200.00 16600.00 0.00 0.19924 19400.00 16600.00 0.00 0.19933 19000.00 16800.00 0.00 0.20227 19200.00 16800.00 0.00 0.20385 19000.00 17000.00 0.00 0.20696 19200.00 17000.00 0.00 0.20838 18800.00 17200.00 0.00 0.20845 19000.00 17200.00 0.00 0.21152 19200.00 17200.00 0.00 0.21276 18800.00 17400.00 0.00 0.21334 19000.00 17400.00 0.00 0.21627 19200.00 17400.00 0.00 0.21730 18800.00 17600.00 0.00 0.21879 19000.00 17600.00 0.00 0.22153 19200.00 17600.00 0.00 0.22229 18800.00 17800.00 0.00 0.22470 19000.00 17800.00 0.00 0.22717 19200.00 17800.00 0.00 0.22760 18800.00 18000.00 0.00 0.23086 19000.00 18000.00 0.00 0.23298 19200.00 18000.00 0.00 0.23299 18600.00 18200.00 0.00 0.23349 18800.00 18200.00 0.00 0.23693 19000.00 18200.00 0.00 0.23858 18600.00 18400.00 0.00 0.23915 18800.00 18400.00 0.00 0.24205 19000.00 18400.00 0.00 0.24317 18600.00 18600.00 0.00 0.24330 18800.00 18600.00 0.00 0.24567 19000.00 18600.00 0.00 0.24623 18800.00 18800.00 0.00 0.24779 18800.00 19000.00 0.00 0.24801 18800.00 19000.00 5.00 0.24813 18800.00 19000.00 10.00 0.24825 18800.00 19000.00 15.00 0.24836 18800.00 19000.00 20.00 0.24845 18800.00 19000.00 25.00 0.24853 18800.00 19000.00 30.00 0.24858 18800.00 19000.00 35.00 0.24862 18800.00 19000.00 40.00 0.24863 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18785.53 18987.11 40.00 0.24864 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/285.ctf NX, NY, NZ: 360 360 1 MODE : real