CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/288.mrc Output diagnostic file name Micrographs/288.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/288.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2426.000 8448.000 Mean, RMS : 4424.415 220.0921 TITLE 1: emi2mrc: 288.emi Sun Jan 19 23:10:17 2014 TITLE 2: kV 300, Mag 78000x, Def 24120, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -492.873, Y 530.958, Z -23.053, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01382 8200.00 8000.00 0.00 -0.01340 8400.00 8000.00 0.00 -0.01293 8600.00 8000.00 0.00 -0.01254 8800.00 8000.00 0.00 -0.01231 9000.00 8000.00 0.00 -0.01217 9200.00 8000.00 0.00 -0.01203 9400.00 8000.00 0.00 -0.01179 9600.00 8000.00 0.00 -0.01140 9800.00 8000.00 0.00 -0.01089 10000.00 8000.00 0.00 -0.01037 10200.00 8000.00 0.00 -0.00989 10400.00 8000.00 0.00 -0.00947 10600.00 8000.00 0.00 -0.00909 10800.00 8000.00 0.00 -0.00871 11000.00 8000.00 0.00 -0.00827 11200.00 8000.00 0.00 -0.00776 11400.00 8000.00 0.00 -0.00715 11600.00 8000.00 0.00 -0.00640 11800.00 8000.00 0.00 -0.00548 12000.00 8000.00 0.00 -0.00436 12200.00 8000.00 0.00 -0.00306 12400.00 8000.00 0.00 -0.00165 12600.00 8000.00 0.00 -0.00020 12800.00 8000.00 0.00 0.00127 13000.00 8000.00 0.00 0.00276 13200.00 8000.00 0.00 0.00433 13400.00 8000.00 0.00 0.00603 13600.00 8000.00 0.00 0.00790 13800.00 8000.00 0.00 0.00992 14000.00 8000.00 0.00 0.01202 14200.00 8000.00 0.00 0.01411 14400.00 8000.00 0.00 0.01610 14600.00 8000.00 0.00 0.01796 14800.00 8000.00 0.00 0.01969 15000.00 8000.00 0.00 0.02142 15200.00 8000.00 0.00 0.02327 15400.00 8000.00 0.00 0.02536 15600.00 8000.00 0.00 0.02775 15800.00 8000.00 0.00 0.03043 16000.00 8000.00 0.00 0.03327 16200.00 8000.00 0.00 0.03617 16400.00 8000.00 0.00 0.03902 16600.00 8000.00 0.00 0.04178 16800.00 8000.00 0.00 0.04447 17000.00 8000.00 0.00 0.04719 17200.00 8000.00 0.00 0.05000 17400.00 8000.00 0.00 0.05293 17600.00 8000.00 0.00 0.05598 17800.00 8000.00 0.00 0.05911 18000.00 8000.00 0.00 0.06225 18200.00 8000.00 0.00 0.06542 18400.00 8000.00 0.00 0.06869 18600.00 8000.00 0.00 0.07209 18800.00 8000.00 0.00 0.07562 19000.00 8000.00 0.00 0.07915 19200.00 8000.00 0.00 0.08239 19400.00 8000.00 0.00 0.08506 19600.00 8000.00 0.00 0.08696 19800.00 8000.00 0.00 0.08809 20000.00 8000.00 0.00 0.08864 20200.00 8000.00 0.00 0.08886 20400.00 8000.00 0.00 0.08891 20000.00 8200.00 0.00 0.08932 20200.00 8200.00 0.00 0.08952 20400.00 8200.00 0.00 0.08956 20000.00 8400.00 0.00 0.08992 20200.00 8400.00 0.00 0.09011 20400.00 8400.00 0.00 0.09015 19800.00 8600.00 0.00 0.09018 20000.00 8600.00 0.00 0.09069 20200.00 8600.00 0.00 0.09088 20400.00 8600.00 0.00 0.09092 19800.00 8800.00 0.00 0.09119 20000.00 8800.00 0.00 0.09170 20200.00 8800.00 0.00 0.09190 20400.00 8800.00 0.00 0.09193 19800.00 9000.00 0.00 0.09237 20000.00 9000.00 0.00 0.09288 20200.00 9000.00 0.00 0.09308 20400.00 9000.00 0.00 0.09310 19800.00 9200.00 0.00 0.09364 20000.00 9200.00 0.00 0.09415 20200.00 9200.00 0.00 0.09434 20400.00 9200.00 0.00 0.09435 19800.00 9400.00 0.00 0.09490 20000.00 9400.00 0.00 0.09540 20200.00 9400.00 0.00 0.09558 19800.00 9600.00 0.00 0.09610 20000.00 9600.00 0.00 0.09660 20200.00 9600.00 0.00 0.09675 19800.00 9800.00 0.00 0.09739 20000.00 9800.00 0.00 0.09787 20200.00 9800.00 0.00 0.09799 19800.00 10000.00 0.00 0.09887 20000.00 10000.00 0.00 0.09932 20200.00 10000.00 0.00 0.09940 19600.00 10200.00 0.00 0.09945 19800.00 10200.00 0.00 0.10052 20000.00 10200.00 0.00 0.10093 20200.00 10200.00 0.00 0.10097 19600.00 10400.00 0.00 0.10124 19800.00 10400.00 0.00 0.10228 20000.00 10400.00 0.00 0.10265 19600.00 10600.00 0.00 0.10310 19800.00 10600.00 0.00 0.10410 20000.00 10600.00 0.00 0.10442 19600.00 10800.00 0.00 0.10491 19800.00 10800.00 0.00 0.10587 20000.00 10800.00 0.00 0.10614 19600.00 11000.00 0.00 0.10674 19800.00 11000.00 0.00 0.10764 20000.00 11000.00 0.00 0.10786 19600.00 11200.00 0.00 0.10865 19800.00 11200.00 0.00 0.10950 20000.00 11200.00 0.00 0.10966 19600.00 11400.00 0.00 0.11061 19800.00 11400.00 0.00 0.11140 20000.00 11400.00 0.00 0.11150 19600.00 11600.00 0.00 0.11258 19800.00 11600.00 0.00 0.11332 20000.00 11600.00 0.00 0.11336 19600.00 11800.00 0.00 0.11457 19800.00 11800.00 0.00 0.11526 19600.00 12000.00 0.00 0.11654 19800.00 12000.00 0.00 0.11718 19600.00 12200.00 0.00 0.11854 19800.00 12200.00 0.00 0.11914 19600.00 12400.00 0.00 0.12072 19800.00 12400.00 0.00 0.12129 19400.00 12600.00 0.00 0.12150 19600.00 12600.00 0.00 0.12305 19800.00 12600.00 0.00 0.12358 19400.00 12800.00 0.00 0.12390 19600.00 12800.00 0.00 0.12542 19800.00 12800.00 0.00 0.12592 19400.00 13000.00 0.00 0.12630 19600.00 13000.00 0.00 0.12780 19800.00 13000.00 0.00 0.12828 19400.00 13200.00 0.00 0.12868 19600.00 13200.00 0.00 0.13017 19800.00 13200.00 0.00 0.13063 19400.00 13400.00 0.00 0.13106 19600.00 13400.00 0.00 0.13255 19800.00 13400.00 0.00 0.13299 19400.00 13600.00 0.00 0.13366 19600.00 13600.00 0.00 0.13514 19800.00 13600.00 0.00 0.13556 19400.00 13800.00 0.00 0.13660 19600.00 13800.00 0.00 0.13807 19800.00 13800.00 0.00 0.13847 19400.00 14000.00 0.00 0.13979 19600.00 14000.00 0.00 0.14125 19800.00 14000.00 0.00 0.14163 19400.00 14200.00 0.00 0.14315 19600.00 14200.00 0.00 0.14460 19800.00 14200.00 0.00 0.14496 19400.00 14400.00 0.00 0.14660 19600.00 14400.00 0.00 0.14802 19800.00 14400.00 0.00 0.14834 19400.00 14600.00 0.00 0.14996 19600.00 14600.00 0.00 0.15135 19800.00 14600.00 0.00 0.15163 19400.00 14800.00 0.00 0.15331 19600.00 14800.00 0.00 0.15466 19800.00 14800.00 0.00 0.15488 19400.00 15000.00 0.00 0.15681 19600.00 15000.00 0.00 0.15812 19800.00 15000.00 0.00 0.15828 19400.00 15200.00 0.00 0.16040 19600.00 15200.00 0.00 0.16166 19800.00 15200.00 0.00 0.16175 19400.00 15400.00 0.00 0.16396 19600.00 15400.00 0.00 0.16518 19800.00 15400.00 0.00 0.16520 19400.00 15600.00 0.00 0.16751 19600.00 15600.00 0.00 0.16870 19400.00 15800.00 0.00 0.17100 19600.00 15800.00 0.00 0.17214 19400.00 16000.00 0.00 0.17443 19600.00 16000.00 0.00 0.17552 19200.00 16200.00 0.00 0.17560 19400.00 16200.00 0.00 0.17806 19600.00 16200.00 0.00 0.17908 19200.00 16400.00 0.00 0.17949 19400.00 16400.00 0.00 0.18192 19600.00 16400.00 0.00 0.18287 19200.00 16600.00 0.00 0.18345 19400.00 16600.00 0.00 0.18584 19600.00 16600.00 0.00 0.18670 19200.00 16800.00 0.00 0.18743 19400.00 16800.00 0.00 0.18977 19600.00 16800.00 0.00 0.19053 19200.00 17000.00 0.00 0.19139 19400.00 17000.00 0.00 0.19368 19600.00 17000.00 0.00 0.19431 19200.00 17200.00 0.00 0.19529 19400.00 17200.00 0.00 0.19750 19600.00 17200.00 0.00 0.19800 19200.00 17400.00 0.00 0.19937 19400.00 17400.00 0.00 0.20148 19600.00 17400.00 0.00 0.20179 19200.00 17600.00 0.00 0.20381 19400.00 17600.00 0.00 0.20577 19600.00 17600.00 0.00 0.20586 19200.00 17800.00 0.00 0.20839 19400.00 17800.00 0.00 0.21015 19200.00 18000.00 0.00 0.21295 19400.00 18000.00 0.00 0.21446 19200.00 18200.00 0.00 0.21741 19400.00 18200.00 0.00 0.21858 19200.00 18400.00 0.00 0.22147 19400.00 18400.00 0.00 0.22223 19000.00 18600.00 0.00 0.22251 19200.00 18600.00 0.00 0.22508 19400.00 18600.00 0.00 0.22535 19000.00 18800.00 0.00 0.22629 19200.00 18800.00 0.00 0.22837 19000.00 19000.00 0.00 0.22933 19200.00 19000.00 0.00 0.23072 19200.00 19200.00 0.00 0.23130 19000.00 19400.00 35.00 0.23130 19000.00 19400.00 40.00 0.23132 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19084.67 19279.96 40.29 0.23174 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/288.ctf NX, NY, NZ: 360 360 1 MODE : real