CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/290.mrc Output diagnostic file name Micrographs/290.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/290.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2502.000 8654.000 Mean, RMS : 4417.639 223.5597 TITLE 1: emi2mrc: 290.emi Sun Jan 19 23:14:44 2014 TITLE 2: kV 300, Mag 78000x, Def 23460, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -492.774, Y 535.028, Z -23.053, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.02315 9000.00 8000.00 0.00 -0.02311 9200.00 8000.00 0.00 -0.02291 9400.00 8000.00 0.00 -0.02268 9600.00 8000.00 0.00 -0.02244 9800.00 8000.00 0.00 -0.02223 10000.00 8000.00 0.00 -0.02210 10200.00 8000.00 0.00 -0.02199 10400.00 8000.00 0.00 -0.02183 10600.00 8000.00 0.00 -0.02161 10800.00 8000.00 0.00 -0.02137 11000.00 8000.00 0.00 -0.02116 11200.00 8000.00 0.00 -0.02104 11400.00 8000.00 0.00 -0.02099 11600.00 8000.00 0.00 -0.02092 11800.00 8000.00 0.00 -0.02075 12000.00 8000.00 0.00 -0.02042 12200.00 8000.00 0.00 -0.01994 12400.00 8000.00 0.00 -0.01936 12600.00 8000.00 0.00 -0.01877 12800.00 8000.00 0.00 -0.01818 13000.00 8000.00 0.00 -0.01759 13200.00 8000.00 0.00 -0.01696 13400.00 8000.00 0.00 -0.01627 13600.00 8000.00 0.00 -0.01552 13800.00 8000.00 0.00 -0.01473 14000.00 8000.00 0.00 -0.01390 14200.00 8000.00 0.00 -0.01305 14400.00 8000.00 0.00 -0.01217 14600.00 8000.00 0.00 -0.01124 14800.00 8000.00 0.00 -0.01022 15000.00 8000.00 0.00 -0.00905 15200.00 8000.00 0.00 -0.00768 15400.00 8000.00 0.00 -0.00607 15600.00 8000.00 0.00 -0.00425 15800.00 8000.00 0.00 -0.00227 16000.00 8000.00 0.00 -0.00022 16200.00 8000.00 0.00 0.00186 16400.00 8000.00 0.00 0.00398 16600.00 8000.00 0.00 0.00616 16800.00 8000.00 0.00 0.00841 17000.00 8000.00 0.00 0.01070 17200.00 8000.00 0.00 0.01298 17400.00 8000.00 0.00 0.01520 17600.00 8000.00 0.00 0.01735 17800.00 8000.00 0.00 0.01947 18000.00 8000.00 0.00 0.02157 18200.00 8000.00 0.00 0.02366 18400.00 8000.00 0.00 0.02572 18600.00 8000.00 0.00 0.02775 18800.00 8000.00 0.00 0.02979 19000.00 8000.00 0.00 0.03196 19200.00 8000.00 0.00 0.03433 19400.00 8000.00 0.00 0.03696 19600.00 8000.00 0.00 0.03980 19800.00 8000.00 0.00 0.04277 20000.00 8000.00 0.00 0.04581 20200.00 8000.00 0.00 0.04895 20400.00 8000.00 0.00 0.05224 20600.00 8000.00 0.00 0.05575 20800.00 8000.00 0.00 0.05945 21000.00 8000.00 0.00 0.06311 21200.00 8000.00 0.00 0.06644 21400.00 8000.00 0.00 0.06915 21600.00 8000.00 0.00 0.07102 21800.00 8000.00 0.00 0.07204 22000.00 8000.00 0.00 0.07237 21800.00 8200.00 0.00 0.07259 22000.00 8200.00 0.00 0.07292 21800.00 8400.00 0.00 0.07313 22000.00 8400.00 0.00 0.07345 21800.00 8600.00 0.00 0.07381 22000.00 8600.00 0.00 0.07412 21800.00 8800.00 0.00 0.07463 22000.00 8800.00 0.00 0.07493 21800.00 9000.00 0.00 0.07552 22000.00 9000.00 0.00 0.07582 21800.00 9200.00 0.00 0.07645 22000.00 9200.00 0.00 0.07676 21800.00 9400.00 0.00 0.07741 22000.00 9400.00 0.00 0.07773 21800.00 9600.00 0.00 0.07842 22000.00 9600.00 0.00 0.07876 21800.00 9800.00 0.00 0.07963 22000.00 9800.00 0.00 0.07998 21600.00 10000.00 0.00 0.08000 21800.00 10000.00 0.00 0.08107 22000.00 10000.00 0.00 0.08142 21600.00 10200.00 0.00 0.08155 21800.00 10200.00 0.00 0.08262 22000.00 10200.00 0.00 0.08296 21600.00 10400.00 0.00 0.08311 21800.00 10400.00 0.00 0.08416 22000.00 10400.00 0.00 0.08447 21600.00 10600.00 0.00 0.08455 21800.00 10600.00 0.00 0.08557 22000.00 10600.00 0.00 0.08586 21800.00 10800.00 0.00 0.08679 22000.00 10800.00 0.00 0.08703 21800.00 11000.00 0.00 0.08787 22000.00 11000.00 0.00 0.08808 21800.00 11200.00 0.00 0.08896 22000.00 11200.00 0.00 0.08915 21600.00 11400.00 0.00 0.08917 21800.00 11400.00 0.00 0.09009 22000.00 11400.00 0.00 0.09028 21600.00 11600.00 0.00 0.09036 21800.00 11600.00 0.00 0.09128 22000.00 11600.00 0.00 0.09147 21600.00 11800.00 0.00 0.09164 21800.00 11800.00 0.00 0.09256 22000.00 11800.00 0.00 0.09275 21600.00 12000.00 0.00 0.09301 21800.00 12000.00 0.00 0.09393 22000.00 12000.00 0.00 0.09412 21600.00 12200.00 0.00 0.09448 21800.00 12200.00 0.00 0.09540 22000.00 12200.00 0.00 0.09558 21600.00 12400.00 0.00 0.09612 21800.00 12400.00 0.00 0.09702 22000.00 12400.00 0.00 0.09718 21600.00 12600.00 0.00 0.09785 21800.00 12600.00 0.00 0.09873 22000.00 12600.00 0.00 0.09887 21600.00 12800.00 0.00 0.09953 21800.00 12800.00 0.00 0.10038 22000.00 12800.00 0.00 0.10050 21600.00 13000.00 0.00 0.10113 21800.00 13000.00 0.00 0.10195 22000.00 13000.00 0.00 0.10205 21600.00 13200.00 0.00 0.10267 21800.00 13200.00 0.00 0.10347 22000.00 13200.00 0.00 0.10354 21600.00 13400.00 0.00 0.10426 21800.00 13400.00 0.00 0.10504 22000.00 13400.00 0.00 0.10511 21600.00 13600.00 0.00 0.10611 21800.00 13600.00 0.00 0.10688 22000.00 13600.00 0.00 0.10696 21600.00 13800.00 0.00 0.10828 21800.00 13800.00 0.00 0.10907 22000.00 13800.00 0.00 0.10916 21600.00 14000.00 0.00 0.11065 21800.00 14000.00 0.00 0.11145 22000.00 14000.00 0.00 0.11156 21600.00 14200.00 0.00 0.11308 21800.00 14200.00 0.00 0.11389 22000.00 14200.00 0.00 0.11400 21600.00 14400.00 0.00 0.11544 21800.00 14400.00 0.00 0.11626 22000.00 14400.00 0.00 0.11637 21600.00 14600.00 0.00 0.11769 21800.00 14600.00 0.00 0.11852 22000.00 14600.00 0.00 0.11862 21600.00 14800.00 0.00 0.11999 21800.00 14800.00 0.00 0.12083 22000.00 14800.00 0.00 0.12091 21600.00 15000.00 0.00 0.12254 21800.00 15000.00 0.00 0.12337 22000.00 15000.00 0.00 0.12341 21400.00 15200.00 0.00 0.12344 21600.00 15200.00 0.00 0.12527 21800.00 15200.00 0.00 0.12608 21400.00 15400.00 0.00 0.12621 21600.00 15400.00 0.00 0.12804 21800.00 15400.00 0.00 0.12881 21400.00 15600.00 0.00 0.12895 21600.00 15600.00 0.00 0.13075 21800.00 15600.00 0.00 0.13148 21400.00 15800.00 0.00 0.13153 21600.00 15800.00 0.00 0.13331 21800.00 15800.00 0.00 0.13398 21400.00 16000.00 0.00 0.13403 21600.00 16000.00 0.00 0.13578 21800.00 16000.00 0.00 0.13640 21400.00 16200.00 0.00 0.13670 21600.00 16200.00 0.00 0.13842 21800.00 16200.00 0.00 0.13898 21400.00 16400.00 0.00 0.13961 21600.00 16400.00 0.00 0.14129 21800.00 16400.00 0.00 0.14179 21400.00 16600.00 0.00 0.14262 21600.00 16600.00 0.00 0.14426 21800.00 16600.00 0.00 0.14469 21400.00 16800.00 0.00 0.14563 21600.00 16800.00 0.00 0.14723 21800.00 16800.00 0.00 0.14760 21400.00 17000.00 0.00 0.14851 21600.00 17000.00 0.00 0.15007 21800.00 17000.00 0.00 0.15039 21400.00 17200.00 0.00 0.15118 21600.00 17200.00 0.00 0.15271 21800.00 17200.00 0.00 0.15299 21400.00 17400.00 0.00 0.15387 21600.00 17400.00 0.00 0.15538 21800.00 17400.00 0.00 0.15560 21400.00 17600.00 0.00 0.15680 21600.00 17600.00 0.00 0.15829 21800.00 17600.00 0.00 0.15845 21400.00 17800.00 0.00 0.15992 21600.00 17800.00 0.00 0.16137 21800.00 17800.00 0.00 0.16145 21400.00 18000.00 0.00 0.16314 21600.00 18000.00 0.00 0.16453 21400.00 18200.00 0.00 0.16641 21600.00 18200.00 0.00 0.16773 21400.00 18400.00 0.00 0.16959 21600.00 18400.00 0.00 0.17083 21400.00 18600.00 0.00 0.17282 21600.00 18600.00 0.00 0.17395 21400.00 18800.00 0.00 0.17635 21600.00 18800.00 0.00 0.17735 21200.00 19000.00 0.00 0.17766 21400.00 19000.00 0.00 0.18016 21600.00 19000.00 0.00 0.18100 21200.00 19200.00 0.00 0.18170 21400.00 19200.00 0.00 0.18406 21600.00 19200.00 0.00 0.18472 21200.00 19400.00 0.00 0.18570 21400.00 19400.00 0.00 0.18791 21600.00 19400.00 0.00 0.18838 21200.00 19600.00 0.00 0.18944 21400.00 19600.00 0.00 0.19149 21600.00 19600.00 0.00 0.19176 21200.00 19800.00 0.00 0.19291 21400.00 19800.00 0.00 0.19479 21600.00 19800.00 0.00 0.19483 21200.00 20000.00 0.00 0.19651 21400.00 20000.00 0.00 0.19816 21200.00 20200.00 0.00 0.20041 21400.00 20200.00 0.00 0.20176 21200.00 20400.00 0.00 0.20442 21400.00 20400.00 0.00 0.20539 21000.00 20600.00 0.00 0.20578 21200.00 20600.00 0.00 0.20830 21400.00 20600.00 0.00 0.20882 21000.00 20800.00 0.00 0.20962 21200.00 20800.00 0.00 0.21159 21000.00 21000.00 0.00 0.21215 21200.00 21000.00 0.00 0.21354 21200.00 21200.00 0.00 0.21405 21000.00 21400.00 25.00 0.21413 21000.00 21400.00 30.00 0.21430 21000.00 21400.00 35.00 0.21445 21000.00 21400.00 40.00 0.21455 21000.00 21400.00 45.00 0.21461 21000.00 21400.00 50.00 0.21461 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21057.11 21380.18 46.45 0.21468 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/290.ctf NX, NY, NZ: 360 360 1 MODE : real