CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/291.mrc Output diagnostic file name Micrographs/291.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/291.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2398.000 7756.000 Mean, RMS : 4409.346 222.5162 TITLE 1: emi2mrc: 291.emi Sun Jan 19 23:16:41 2014 TITLE 2: kV 300, Mag 78000x, Def 23460, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -489.172, Y 539.139, Z -23.053, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.04175 13000.00 8000.00 0.00 -0.04164 13200.00 8000.00 0.00 -0.04124 13400.00 8000.00 0.00 -0.04064 13600.00 8000.00 0.00 -0.03991 13800.00 8000.00 0.00 -0.03923 14000.00 8000.00 0.00 -0.03868 14200.00 8000.00 0.00 -0.03830 14400.00 8000.00 0.00 -0.03803 14600.00 8000.00 0.00 -0.03776 14800.00 8000.00 0.00 -0.03738 15000.00 8000.00 0.00 -0.03687 15200.00 8000.00 0.00 -0.03620 15400.00 8000.00 0.00 -0.03537 15600.00 8000.00 0.00 -0.03438 15800.00 8000.00 0.00 -0.03323 16000.00 8000.00 0.00 -0.03195 16200.00 8000.00 0.00 -0.03058 16400.00 8000.00 0.00 -0.02919 16600.00 8000.00 0.00 -0.02779 16800.00 8000.00 0.00 -0.02639 17000.00 8000.00 0.00 -0.02495 17200.00 8000.00 0.00 -0.02347 17400.00 8000.00 0.00 -0.02196 17600.00 8000.00 0.00 -0.02047 17800.00 8000.00 0.00 -0.01900 18000.00 8000.00 0.00 -0.01753 18200.00 8000.00 0.00 -0.01601 18400.00 8000.00 0.00 -0.01438 18600.00 8000.00 0.00 -0.01262 18800.00 8000.00 0.00 -0.01077 19000.00 8000.00 0.00 -0.00889 19200.00 8000.00 0.00 -0.00706 19400.00 8000.00 0.00 -0.00530 19600.00 8000.00 0.00 -0.00361 19800.00 8000.00 0.00 -0.00194 20000.00 8000.00 0.00 -0.00023 20200.00 8000.00 0.00 0.00159 20400.00 8000.00 0.00 0.00355 20600.00 8000.00 0.00 0.00562 20800.00 8000.00 0.00 0.00777 21000.00 8000.00 0.00 0.00994 21200.00 8000.00 0.00 0.01207 21400.00 8000.00 0.00 0.01419 21600.00 8000.00 0.00 0.01630 21800.00 8000.00 0.00 0.01845 22000.00 8000.00 0.00 0.02066 22200.00 8000.00 0.00 0.02293 22400.00 8000.00 0.00 0.02528 22600.00 8000.00 0.00 0.02772 22800.00 8000.00 0.00 0.03031 23000.00 8000.00 0.00 0.03308 23200.00 8000.00 0.00 0.03602 23400.00 8000.00 0.00 0.03905 23600.00 8000.00 0.00 0.04197 23800.00 8000.00 0.00 0.04457 24000.00 8000.00 0.00 0.04665 24200.00 8000.00 0.00 0.04807 24400.00 8000.00 0.00 0.04883 24600.00 8000.00 0.00 0.04905 24400.00 8200.00 0.00 0.04933 24600.00 8200.00 0.00 0.04952 24400.00 8400.00 0.00 0.04995 24600.00 8400.00 0.00 0.05012 24400.00 8600.00 0.00 0.05066 24600.00 8600.00 0.00 0.05081 24400.00 8800.00 0.00 0.05136 24600.00 8800.00 0.00 0.05149 24400.00 9000.00 0.00 0.05196 24600.00 9000.00 0.00 0.05208 24400.00 9200.00 0.00 0.05245 24600.00 9200.00 0.00 0.05256 24400.00 9400.00 0.00 0.05288 24600.00 9400.00 0.00 0.05299 24400.00 9600.00 0.00 0.05332 24600.00 9600.00 0.00 0.05342 24400.00 9800.00 0.00 0.05387 24600.00 9800.00 0.00 0.05397 24400.00 10000.00 0.00 0.05456 24600.00 10000.00 0.00 0.05466 24200.00 10200.00 0.00 0.05468 24400.00 10200.00 0.00 0.05531 24600.00 10200.00 0.00 0.05541 24200.00 10400.00 0.00 0.05546 24400.00 10400.00 0.00 0.05609 24600.00 10400.00 0.00 0.05617 24200.00 10600.00 0.00 0.05625 24400.00 10600.00 0.00 0.05685 24600.00 10600.00 0.00 0.05691 24200.00 10800.00 0.00 0.05702 24400.00 10800.00 0.00 0.05760 24600.00 10800.00 0.00 0.05764 24200.00 11000.00 0.00 0.05785 24400.00 11000.00 0.00 0.05839 24600.00 11000.00 0.00 0.05840 24200.00 11200.00 0.00 0.05874 24400.00 11200.00 0.00 0.05926 24200.00 11400.00 0.00 0.05965 24400.00 11400.00 0.00 0.06013 24200.00 11600.00 0.00 0.06053 24400.00 11600.00 0.00 0.06099 24200.00 11800.00 0.00 0.06138 24400.00 11800.00 0.00 0.06184 24200.00 12000.00 0.00 0.06226 24400.00 12000.00 0.00 0.06272 24200.00 12200.00 0.00 0.06324 24400.00 12200.00 0.00 0.06371 24200.00 12400.00 0.00 0.06442 24400.00 12400.00 0.00 0.06491 24200.00 12600.00 0.00 0.06576 24400.00 12600.00 0.00 0.06625 24200.00 12800.00 0.00 0.06712 24400.00 12800.00 0.00 0.06761 24200.00 13000.00 0.00 0.06842 24400.00 13000.00 0.00 0.06890 24200.00 13200.00 0.00 0.06960 24400.00 13200.00 0.00 0.07008 24200.00 13400.00 0.00 0.07071 24400.00 13400.00 0.00 0.07117 24200.00 13600.00 0.00 0.07188 24400.00 13600.00 0.00 0.07232 24200.00 13800.00 0.00 0.07319 24400.00 13800.00 0.00 0.07363 24200.00 14000.00 0.00 0.07462 24400.00 14000.00 0.00 0.07505 24200.00 14200.00 0.00 0.07610 24400.00 14200.00 0.00 0.07653 24200.00 14400.00 0.00 0.07760 24400.00 14400.00 0.00 0.07801 24200.00 14600.00 0.00 0.07906 24400.00 14600.00 0.00 0.07947 24200.00 14800.00 0.00 0.08059 24400.00 14800.00 0.00 0.08098 24000.00 15000.00 0.00 0.08114 24200.00 15000.00 0.00 0.08229 24400.00 15000.00 0.00 0.08267 24000.00 15200.00 0.00 0.08304 24200.00 15200.00 0.00 0.08417 24400.00 15200.00 0.00 0.08454 24000.00 15400.00 0.00 0.08506 24200.00 15400.00 0.00 0.08618 24400.00 15400.00 0.00 0.08652 24000.00 15600.00 0.00 0.08713 24200.00 15600.00 0.00 0.08823 24400.00 15600.00 0.00 0.08854 24000.00 15800.00 0.00 0.08913 24200.00 15800.00 0.00 0.09021 24400.00 15800.00 0.00 0.09049 24000.00 16000.00 0.00 0.09105 24200.00 16000.00 0.00 0.09210 24400.00 16000.00 0.00 0.09236 24000.00 16200.00 0.00 0.09298 24200.00 16200.00 0.00 0.09403 24400.00 16200.00 0.00 0.09427 24000.00 16400.00 0.00 0.09500 24200.00 16400.00 0.00 0.09605 24400.00 16400.00 0.00 0.09629 24000.00 16600.00 0.00 0.09712 24200.00 16600.00 0.00 0.09817 24400.00 16600.00 0.00 0.09840 24000.00 16800.00 0.00 0.09936 24200.00 16800.00 0.00 0.10041 24400.00 16800.00 0.00 0.10062 24000.00 17000.00 0.00 0.10168 24200.00 17000.00 0.00 0.10271 24400.00 17000.00 0.00 0.10290 24000.00 17200.00 0.00 0.10402 24200.00 17200.00 0.00 0.10502 24400.00 17200.00 0.00 0.10517 24000.00 17400.00 0.00 0.10640 24200.00 17400.00 0.00 0.10738 24400.00 17400.00 0.00 0.10748 24000.00 17600.00 0.00 0.10885 24200.00 17600.00 0.00 0.10979 24400.00 17600.00 0.00 0.10984 24000.00 17800.00 0.00 0.11128 24200.00 17800.00 0.00 0.11218 24000.00 18000.00 0.00 0.11366 24200.00 18000.00 0.00 0.11451 24000.00 18200.00 0.00 0.11602 24200.00 18200.00 0.00 0.11682 24000.00 18400.00 0.00 0.11835 24200.00 18400.00 0.00 0.11909 24000.00 18600.00 0.00 0.12072 24200.00 18600.00 0.00 0.12142 23800.00 18800.00 0.00 0.12151 24000.00 18800.00 0.00 0.12324 24200.00 18800.00 0.00 0.12388 23800.00 19000.00 0.00 0.12410 24000.00 19000.00 0.00 0.12577 24200.00 19000.00 0.00 0.12635 23800.00 19200.00 0.00 0.12655 24000.00 19200.00 0.00 0.12816 24200.00 19200.00 0.00 0.12868 23800.00 19400.00 0.00 0.12885 24000.00 19400.00 0.00 0.13042 24200.00 19400.00 0.00 0.13088 23800.00 19600.00 0.00 0.13098 24000.00 19600.00 0.00 0.13251 24200.00 19600.00 0.00 0.13293 23800.00 19800.00 0.00 0.13303 24000.00 19800.00 0.00 0.13452 24200.00 19800.00 0.00 0.13490 23800.00 20000.00 0.00 0.13523 24000.00 20000.00 0.00 0.13670 24200.00 20000.00 0.00 0.13703 23800.00 20200.00 0.00 0.13766 24000.00 20200.00 0.00 0.13909 24200.00 20200.00 0.00 0.13937 23800.00 20400.00 0.00 0.14013 24000.00 20400.00 0.00 0.14152 24200.00 20400.00 0.00 0.14173 23800.00 20600.00 0.00 0.14253 24000.00 20600.00 0.00 0.14388 24200.00 20600.00 0.00 0.14402 23800.00 20800.00 0.00 0.14478 24000.00 20800.00 0.00 0.14609 24200.00 20800.00 0.00 0.14616 23800.00 21000.00 0.00 0.14684 24000.00 21000.00 0.00 0.14810 23800.00 21200.00 0.00 0.14891 24000.00 21200.00 0.00 0.15011 23800.00 21400.00 0.00 0.15118 24000.00 21400.00 0.00 0.15229 23800.00 21600.00 0.00 0.15356 24000.00 21600.00 0.00 0.15453 23800.00 21800.00 0.00 0.15589 24000.00 21800.00 0.00 0.15670 23800.00 22000.00 0.00 0.15807 24000.00 22000.00 0.00 0.15868 23800.00 22200.00 0.00 0.15994 24000.00 22200.00 0.00 0.16034 23800.00 22400.00 0.00 0.16163 24000.00 22400.00 0.00 0.16180 23600.00 22600.00 0.00 0.16189 23800.00 22600.00 0.00 0.16342 23600.00 22800.00 0.00 0.16406 23800.00 22800.00 0.00 0.16530 23600.00 23000.00 0.00 0.16615 23800.00 23000.00 0.00 0.16702 23600.00 23200.00 0.00 0.16798 23800.00 23200.00 0.00 0.16841 23600.00 23400.00 0.00 0.16914 23600.00 23600.00 0.00 0.16928 23600.00 23800.00 40.00 0.16930 23600.00 23800.00 45.00 0.16933 23400.00 23800.00 50.00 0.16940 23400.00 23800.00 55.00 0.16951 23400.00 23800.00 60.00 0.16957 23400.00 23800.00 65.00 0.16959 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23468.37 23749.48 65.00 0.16967 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/291.ctf NX, NY, NZ: 360 360 1 MODE : real