CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/294.mrc Output diagnostic file name Micrographs/294.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/294.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2300.000 7916.000 Mean, RMS : 4436.748 227.6035 TITLE 1: emi2mrc: 294.emi Sun Jan 19 23:20:18 2014 TITLE 2: kV 300, Mag 78000x, Def 25920, Spot 4, Int 55.12%, Obj 88.06%, Dif 73.67% TITLE 3: X -484.821, Y 545.168, Z -22.333, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 108 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01225 8200.00 8000.00 0.00 -0.01171 8400.00 8000.00 0.00 -0.01100 8600.00 8000.00 0.00 -0.01028 8800.00 8000.00 0.00 -0.00964 9000.00 8000.00 0.00 -0.00910 9200.00 8000.00 0.00 -0.00862 9400.00 8000.00 0.00 -0.00817 9600.00 8000.00 0.00 -0.00770 9800.00 8000.00 0.00 -0.00716 10000.00 8000.00 0.00 -0.00652 10200.00 8000.00 0.00 -0.00574 10400.00 8000.00 0.00 -0.00485 10600.00 8000.00 0.00 -0.00388 10800.00 8000.00 0.00 -0.00292 11000.00 8000.00 0.00 -0.00199 11200.00 8000.00 0.00 -0.00109 11400.00 8000.00 0.00 -0.00010 11600.00 8000.00 0.00 0.00110 11800.00 8000.00 0.00 0.00259 12000.00 8000.00 0.00 0.00434 12200.00 8000.00 0.00 0.00626 12400.00 8000.00 0.00 0.00824 12600.00 8000.00 0.00 0.01024 12800.00 8000.00 0.00 0.01226 13000.00 8000.00 0.00 0.01434 13200.00 8000.00 0.00 0.01654 13400.00 8000.00 0.00 0.01883 13600.00 8000.00 0.00 0.02122 13800.00 8000.00 0.00 0.02370 14000.00 8000.00 0.00 0.02627 14200.00 8000.00 0.00 0.02895 14400.00 8000.00 0.00 0.03175 14600.00 8000.00 0.00 0.03465 14800.00 8000.00 0.00 0.03762 15000.00 8000.00 0.00 0.04068 15200.00 8000.00 0.00 0.04385 15400.00 8000.00 0.00 0.04714 15600.00 8000.00 0.00 0.05055 15800.00 8000.00 0.00 0.05406 16000.00 8000.00 0.00 0.05760 16200.00 8000.00 0.00 0.06122 16400.00 8000.00 0.00 0.06502 16600.00 8000.00 0.00 0.06905 16800.00 8000.00 0.00 0.07333 17000.00 8000.00 0.00 0.07776 17200.00 8000.00 0.00 0.08206 17400.00 8000.00 0.00 0.08590 17600.00 8000.00 0.00 0.08904 17800.00 8000.00 0.00 0.09132 18000.00 8000.00 0.00 0.09276 18200.00 8000.00 0.00 0.09353 18400.00 8000.00 0.00 0.09385 18600.00 8000.00 0.00 0.09394 18800.00 8000.00 0.00 0.09398 19000.00 8000.00 0.00 0.09402 18000.00 8200.00 0.00 0.09439 18200.00 8200.00 0.00 0.09517 18400.00 8200.00 0.00 0.09550 18600.00 8200.00 0.00 0.09559 18800.00 8200.00 0.00 0.09562 19000.00 8200.00 0.00 0.09565 18000.00 8400.00 0.00 0.09609 18200.00 8400.00 0.00 0.09688 18400.00 8400.00 0.00 0.09720 18600.00 8400.00 0.00 0.09727 18800.00 8400.00 0.00 0.09728 18000.00 8600.00 0.00 0.09800 18200.00 8600.00 0.00 0.09877 18400.00 8600.00 0.00 0.09907 18600.00 8600.00 0.00 0.09911 18000.00 8800.00 0.00 0.10008 18200.00 8800.00 0.00 0.10084 18400.00 8800.00 0.00 0.10110 18000.00 9000.00 0.00 0.10220 18200.00 9000.00 0.00 0.10293 18400.00 9000.00 0.00 0.10315 18000.00 9200.00 0.00 0.10431 18200.00 9200.00 0.00 0.10500 18400.00 9200.00 0.00 0.10518 18000.00 9400.00 0.00 0.10636 18200.00 9400.00 0.00 0.10701 18400.00 9400.00 0.00 0.10714 18000.00 9600.00 0.00 0.10839 18200.00 9600.00 0.00 0.10900 18400.00 9600.00 0.00 0.10908 17800.00 9800.00 0.00 0.10924 18000.00 9800.00 0.00 0.11059 18200.00 9800.00 0.00 0.11115 18400.00 9800.00 0.00 0.11119 17800.00 10000.00 0.00 0.11171 18000.00 10000.00 0.00 0.11301 18200.00 10000.00 0.00 0.11351 17800.00 10200.00 0.00 0.11425 18000.00 10200.00 0.00 0.11550 18200.00 10200.00 0.00 0.11595 17800.00 10400.00 0.00 0.11676 18000.00 10400.00 0.00 0.11795 18200.00 10400.00 0.00 0.11835 17800.00 10600.00 0.00 0.11915 18000.00 10600.00 0.00 0.12029 18200.00 10600.00 0.00 0.12065 17800.00 10800.00 0.00 0.12141 18000.00 10800.00 0.00 0.12251 18200.00 10800.00 0.00 0.12284 17800.00 11000.00 0.00 0.12374 18000.00 11000.00 0.00 0.12482 18200.00 11000.00 0.00 0.12514 17800.00 11200.00 0.00 0.12635 18000.00 11200.00 0.00 0.12742 18200.00 11200.00 0.00 0.12771 17800.00 11400.00 0.00 0.12916 18000.00 11400.00 0.00 0.13020 18200.00 11400.00 0.00 0.13047 17800.00 11600.00 0.00 0.13197 18000.00 11600.00 0.00 0.13298 18200.00 11600.00 0.00 0.13322 17800.00 11800.00 0.00 0.13470 18000.00 11800.00 0.00 0.13567 18200.00 11800.00 0.00 0.13587 17800.00 12000.00 0.00 0.13725 18000.00 12000.00 0.00 0.13819 18200.00 12000.00 0.00 0.13835 17800.00 12200.00 0.00 0.13976 18000.00 12200.00 0.00 0.14067 18200.00 12200.00 0.00 0.14079 17800.00 12400.00 0.00 0.14256 18000.00 12400.00 0.00 0.14343 18200.00 12400.00 0.00 0.14350 17600.00 12600.00 0.00 0.14377 17800.00 12600.00 0.00 0.14573 18000.00 12600.00 0.00 0.14656 18200.00 12600.00 0.00 0.14659 17600.00 12800.00 0.00 0.14718 17800.00 12800.00 0.00 0.14912 18000.00 12800.00 0.00 0.14993 17600.00 13000.00 0.00 0.15071 17800.00 13000.00 0.00 0.15265 18000.00 13000.00 0.00 0.15344 17600.00 13200.00 0.00 0.15424 17800.00 13200.00 0.00 0.15618 18000.00 13200.00 0.00 0.15695 17600.00 13400.00 0.00 0.15772 17800.00 13400.00 0.00 0.15966 18000.00 13400.00 0.00 0.16040 17600.00 13600.00 0.00 0.16142 17800.00 13600.00 0.00 0.16334 18000.00 13600.00 0.00 0.16403 17600.00 13800.00 0.00 0.16553 17800.00 13800.00 0.00 0.16741 18000.00 13800.00 0.00 0.16803 17600.00 14000.00 0.00 0.16987 17800.00 14000.00 0.00 0.17170 18000.00 14000.00 0.00 0.17221 17600.00 14200.00 0.00 0.17426 17800.00 14200.00 0.00 0.17601 18000.00 14200.00 0.00 0.17642 17600.00 14400.00 0.00 0.17858 17800.00 14400.00 0.00 0.18026 18000.00 14400.00 0.00 0.18055 17600.00 14600.00 0.00 0.18275 17800.00 14600.00 0.00 0.18434 18000.00 14600.00 0.00 0.18451 17600.00 14800.00 0.00 0.18702 17800.00 14800.00 0.00 0.18851 18000.00 14800.00 0.00 0.18854 17400.00 15000.00 0.00 0.18881 17600.00 15000.00 0.00 0.19182 17800.00 15000.00 0.00 0.19318 17400.00 15200.00 0.00 0.19423 17600.00 15200.00 0.00 0.19714 17800.00 15200.00 0.00 0.19834 17400.00 15400.00 0.00 0.19994 17600.00 15400.00 0.00 0.20271 17800.00 15400.00 0.00 0.20372 17400.00 15600.00 0.00 0.20568 17600.00 15600.00 0.00 0.20828 17800.00 15600.00 0.00 0.20908 17400.00 15800.00 0.00 0.21114 17600.00 15800.00 0.00 0.21358 17800.00 15800.00 0.00 0.21416 17400.00 16000.00 0.00 0.21642 17600.00 16000.00 0.00 0.21869 17800.00 16000.00 0.00 0.21902 17400.00 16200.00 0.00 0.22219 17600.00 16200.00 0.00 0.22420 17800.00 16200.00 0.00 0.22421 17200.00 16400.00 0.00 0.22488 17400.00 16400.00 0.00 0.22876 17600.00 16400.00 0.00 0.23039 17200.00 16600.00 0.00 0.23235 17400.00 16600.00 0.00 0.23580 17600.00 16600.00 0.00 0.23687 17200.00 16800.00 0.00 0.23992 17400.00 16800.00 0.00 0.24269 17600.00 16800.00 0.00 0.24304 17200.00 17000.00 0.00 0.24643 17400.00 17000.00 0.00 0.24828 17200.00 17200.00 0.00 0.25033 17400.00 17200.00 0.00 0.25120 17200.00 17400.00 5.00 0.25121 17200.00 17400.00 10.00 0.25126 17200.00 17400.00 15.00 0.25130 17200.00 17400.00 20.00 0.25135 17200.00 17400.00 25.00 0.25138 17200.00 17400.00 30.00 0.25142 17200.00 17400.00 35.00 0.25144 17200.00 17400.00 40.00 0.25146 17200.00 17400.00 45.00 0.25146 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 17286.74 17358.79 48.15 0.25171 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/294.ctf NX, NY, NZ: 360 360 1 MODE : real