CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/304.mrc Output diagnostic file name Micrographs/304.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/304.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2284.000 7676.000 Mean, RMS : 4430.602 222.9325 TITLE 1: emi2mrc: 304.emi Mon Jan 20 00:30:01 2014 TITLE 2: kV 300, Mag 78000x, Def 27890, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -500.239, Y 529.910, Z -22.333, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03421 13800.00 8000.00 0.00 -0.03402 14000.00 8000.00 0.00 -0.03360 14200.00 8000.00 0.00 -0.03322 14400.00 8000.00 0.00 -0.03287 14600.00 8000.00 0.00 -0.03249 14800.00 8000.00 0.00 -0.03203 15000.00 8000.00 0.00 -0.03148 15200.00 8000.00 0.00 -0.03085 15400.00 8000.00 0.00 -0.03015 15600.00 8000.00 0.00 -0.02942 15800.00 8000.00 0.00 -0.02869 16000.00 8000.00 0.00 -0.02798 16200.00 8000.00 0.00 -0.02730 16400.00 8000.00 0.00 -0.02659 16600.00 8000.00 0.00 -0.02577 16800.00 8000.00 0.00 -0.02479 17000.00 8000.00 0.00 -0.02361 17200.00 8000.00 0.00 -0.02228 17400.00 8000.00 0.00 -0.02085 17600.00 8000.00 0.00 -0.01940 17800.00 8000.00 0.00 -0.01795 18000.00 8000.00 0.00 -0.01647 18200.00 8000.00 0.00 -0.01495 18400.00 8000.00 0.00 -0.01341 18600.00 8000.00 0.00 -0.01191 18800.00 8000.00 0.00 -0.01050 19000.00 8000.00 0.00 -0.00919 19200.00 8000.00 0.00 -0.00788 19400.00 8000.00 0.00 -0.00645 19600.00 8000.00 0.00 -0.00477 19800.00 8000.00 0.00 -0.00284 20000.00 8000.00 0.00 -0.00078 20200.00 8000.00 0.00 0.00123 20400.00 8000.00 0.00 0.00304 20600.00 8000.00 0.00 0.00457 20800.00 8000.00 0.00 0.00591 21000.00 8000.00 0.00 0.00723 21200.00 8000.00 0.00 0.00870 21400.00 8000.00 0.00 0.01044 21600.00 8000.00 0.00 0.01245 21800.00 8000.00 0.00 0.01462 22000.00 8000.00 0.00 0.01682 22200.00 8000.00 0.00 0.01894 22400.00 8000.00 0.00 0.02094 22600.00 8000.00 0.00 0.02291 22800.00 8000.00 0.00 0.02499 23000.00 8000.00 0.00 0.02728 23200.00 8000.00 0.00 0.02982 23400.00 8000.00 0.00 0.03253 23600.00 8000.00 0.00 0.03519 23800.00 8000.00 0.00 0.03758 24000.00 8000.00 0.00 0.03952 24200.00 8000.00 0.00 0.04088 24400.00 8000.00 0.00 0.04171 24600.00 8000.00 0.00 0.04214 24800.00 8000.00 0.00 0.04229 25000.00 8000.00 0.00 0.04231 24600.00 8200.00 0.00 0.04244 24800.00 8200.00 0.00 0.04260 25000.00 8200.00 0.00 0.04264 24600.00 8400.00 0.00 0.04285 24800.00 8400.00 0.00 0.04303 25000.00 8400.00 0.00 0.04307 24600.00 8600.00 0.00 0.04351 24800.00 8600.00 0.00 0.04371 25000.00 8600.00 0.00 0.04376 24400.00 8800.00 0.00 0.04390 24600.00 8800.00 0.00 0.04441 24800.00 8800.00 0.00 0.04462 25000.00 8800.00 0.00 0.04468 25200.00 8800.00 0.00 0.04468 24400.00 9000.00 0.00 0.04491 24600.00 9000.00 0.00 0.04543 24800.00 9000.00 0.00 0.04564 25000.00 9000.00 0.00 0.04569 24400.00 9200.00 0.00 0.04590 24600.00 9200.00 0.00 0.04641 24800.00 9200.00 0.00 0.04661 25000.00 9200.00 0.00 0.04665 24400.00 9400.00 0.00 0.04676 24600.00 9400.00 0.00 0.04724 24800.00 9400.00 0.00 0.04741 25000.00 9400.00 0.00 0.04742 24400.00 9600.00 0.00 0.04744 24600.00 9600.00 0.00 0.04790 24800.00 9600.00 0.00 0.04804 24400.00 9800.00 0.00 0.04808 24600.00 9800.00 0.00 0.04850 24800.00 9800.00 0.00 0.04862 24400.00 10000.00 0.00 0.04877 24600.00 10000.00 0.00 0.04918 24800.00 10000.00 0.00 0.04927 24400.00 10200.00 0.00 0.04954 24600.00 10200.00 0.00 0.04993 24800.00 10200.00 0.00 0.05002 24400.00 10400.00 0.00 0.05036 24600.00 10400.00 0.00 0.05075 24800.00 10400.00 0.00 0.05084 24400.00 10600.00 0.00 0.05119 24600.00 10600.00 0.00 0.05159 24800.00 10600.00 0.00 0.05169 24400.00 10800.00 0.00 0.05202 24600.00 10800.00 0.00 0.05243 24800.00 10800.00 0.00 0.05253 24400.00 11000.00 0.00 0.05291 24600.00 11000.00 0.00 0.05332 24800.00 11000.00 0.00 0.05344 24400.00 11200.00 0.00 0.05395 24600.00 11200.00 0.00 0.05437 24800.00 11200.00 0.00 0.05447 24400.00 11400.00 0.00 0.05513 24600.00 11400.00 0.00 0.05554 24800.00 11400.00 0.00 0.05563 24400.00 11600.00 0.00 0.05641 24600.00 11600.00 0.00 0.05679 24800.00 11600.00 0.00 0.05686 24400.00 11800.00 0.00 0.05769 24600.00 11800.00 0.00 0.05805 24800.00 11800.00 0.00 0.05810 24200.00 12000.00 0.00 0.05810 24400.00 12000.00 0.00 0.05891 24600.00 12000.00 0.00 0.05924 24800.00 12000.00 0.00 0.05925 24200.00 12200.00 0.00 0.05929 24400.00 12200.00 0.00 0.06007 24600.00 12200.00 0.00 0.06037 24200.00 12400.00 0.00 0.06051 24400.00 12400.00 0.00 0.06127 24600.00 12400.00 0.00 0.06154 24200.00 12600.00 0.00 0.06179 24400.00 12600.00 0.00 0.06253 24600.00 12600.00 0.00 0.06277 24200.00 12800.00 0.00 0.06310 24400.00 12800.00 0.00 0.06382 24600.00 12800.00 0.00 0.06405 24200.00 13000.00 0.00 0.06442 24400.00 13000.00 0.00 0.06514 24600.00 13000.00 0.00 0.06534 24200.00 13200.00 0.00 0.06572 24400.00 13200.00 0.00 0.06643 24600.00 13200.00 0.00 0.06662 24200.00 13400.00 0.00 0.06700 24400.00 13400.00 0.00 0.06769 24600.00 13400.00 0.00 0.06787 24200.00 13600.00 0.00 0.06835 24400.00 13600.00 0.00 0.06902 24600.00 13600.00 0.00 0.06919 24200.00 13800.00 0.00 0.06986 24400.00 13800.00 0.00 0.07051 24600.00 13800.00 0.00 0.07066 24200.00 14000.00 0.00 0.07151 24400.00 14000.00 0.00 0.07214 24600.00 14000.00 0.00 0.07228 24200.00 14200.00 0.00 0.07325 24400.00 14200.00 0.00 0.07386 24600.00 14200.00 0.00 0.07398 24200.00 14400.00 0.00 0.07502 24400.00 14400.00 0.00 0.07561 24600.00 14400.00 0.00 0.07572 24200.00 14600.00 0.00 0.07675 24400.00 14600.00 0.00 0.07732 24600.00 14600.00 0.00 0.07742 24200.00 14800.00 0.00 0.07851 24400.00 14800.00 0.00 0.07906 24600.00 14800.00 0.00 0.07916 24200.00 15000.00 0.00 0.08038 24400.00 15000.00 0.00 0.08092 24600.00 15000.00 0.00 0.08103 24000.00 15200.00 0.00 0.08117 24200.00 15200.00 0.00 0.08239 24400.00 15200.00 0.00 0.08292 24600.00 15200.00 0.00 0.08303 24000.00 15400.00 0.00 0.08327 24200.00 15400.00 0.00 0.08448 24400.00 15400.00 0.00 0.08500 24600.00 15400.00 0.00 0.08511 24000.00 15600.00 0.00 0.08539 24200.00 15600.00 0.00 0.08657 24400.00 15600.00 0.00 0.08708 24600.00 15600.00 0.00 0.08718 24000.00 15800.00 0.00 0.08743 24200.00 15800.00 0.00 0.08858 24400.00 15800.00 0.00 0.08907 24600.00 15800.00 0.00 0.08918 24000.00 16000.00 0.00 0.08938 24200.00 16000.00 0.00 0.09051 24400.00 16000.00 0.00 0.09100 24600.00 16000.00 0.00 0.09111 24000.00 16200.00 0.00 0.09137 24200.00 16200.00 0.00 0.09249 24400.00 16200.00 0.00 0.09298 24600.00 16200.00 0.00 0.09310 24000.00 16400.00 0.00 0.09345 24200.00 16400.00 0.00 0.09457 24400.00 16400.00 0.00 0.09507 24600.00 16400.00 0.00 0.09519 24000.00 16600.00 0.00 0.09561 24200.00 16600.00 0.00 0.09674 24400.00 16600.00 0.00 0.09724 24600.00 16600.00 0.00 0.09737 24000.00 16800.00 0.00 0.09784 24200.00 16800.00 0.00 0.09897 24400.00 16800.00 0.00 0.09947 24600.00 16800.00 0.00 0.09959 24000.00 17000.00 0.00 0.10006 24200.00 17000.00 0.00 0.10119 24400.00 17000.00 0.00 0.10168 24600.00 17000.00 0.00 0.10178 24000.00 17200.00 0.00 0.10221 24200.00 17200.00 0.00 0.10332 24400.00 17200.00 0.00 0.10380 24600.00 17200.00 0.00 0.10387 24000.00 17400.00 0.00 0.10439 24200.00 17400.00 0.00 0.10550 24400.00 17400.00 0.00 0.10595 24600.00 17400.00 0.00 0.10596 24000.00 17600.00 0.00 0.10670 24200.00 17600.00 0.00 0.10778 24400.00 17600.00 0.00 0.10819 24000.00 17800.00 0.00 0.10908 24200.00 17800.00 0.00 0.11012 24400.00 17800.00 0.00 0.11047 24000.00 18000.00 0.00 0.11147 24200.00 18000.00 0.00 0.11246 24400.00 18000.00 0.00 0.11274 24000.00 18200.00 0.00 0.11379 24200.00 18200.00 0.00 0.11473 24400.00 18200.00 0.00 0.11494 24000.00 18400.00 0.00 0.11595 24200.00 18400.00 0.00 0.11683 24400.00 18400.00 0.00 0.11699 24000.00 18600.00 0.00 0.11800 24200.00 18600.00 0.00 0.11885 24400.00 18600.00 0.00 0.11897 24000.00 18800.00 0.00 0.12013 24200.00 18800.00 0.00 0.12096 24400.00 18800.00 0.00 0.12105 24000.00 19000.00 0.00 0.12238 24200.00 19000.00 0.00 0.12319 24400.00 19000.00 0.00 0.12327 24000.00 19200.00 0.00 0.12475 24200.00 19200.00 0.00 0.12556 24400.00 19200.00 0.00 0.12563 24000.00 19400.00 0.00 0.12728 24200.00 19400.00 0.00 0.12809 24400.00 19400.00 0.00 0.12814 23800.00 19600.00 0.00 0.12815 24000.00 19600.00 0.00 0.12989 24200.00 19600.00 0.00 0.13068 24400.00 19600.00 0.00 0.13071 23800.00 19800.00 0.00 0.13078 24000.00 19800.00 0.00 0.13251 24200.00 19800.00 0.00 0.13327 23800.00 20000.00 0.00 0.13357 24000.00 20000.00 0.00 0.13526 24200.00 20000.00 0.00 0.13597 23800.00 20200.00 0.00 0.13650 24000.00 20200.00 0.00 0.13815 24200.00 20200.00 0.00 0.13878 23800.00 20400.00 0.00 0.13944 24000.00 20400.00 0.00 0.14102 24200.00 20400.00 0.00 0.14157 23800.00 20600.00 0.00 0.14234 24000.00 20600.00 0.00 0.14386 24200.00 20600.00 0.00 0.14432 23800.00 20800.00 0.00 0.14519 24000.00 20800.00 0.00 0.14665 24200.00 20800.00 0.00 0.14705 23800.00 21000.00 0.00 0.14795 24000.00 21000.00 0.00 0.14937 24200.00 21000.00 0.00 0.14969 23800.00 21200.00 0.00 0.15081 24000.00 21200.00 0.00 0.15217 24200.00 21200.00 0.00 0.15242 23800.00 21400.00 0.00 0.15391 24000.00 21400.00 0.00 0.15520 24200.00 21400.00 0.00 0.15536 23800.00 21600.00 0.00 0.15715 24000.00 21600.00 0.00 0.15834 24200.00 21600.00 0.00 0.15838 23800.00 21800.00 0.00 0.16037 24000.00 21800.00 0.00 0.16145 23800.00 22000.00 0.00 0.16348 24000.00 22000.00 0.00 0.16443 23800.00 22200.00 0.00 0.16633 24000.00 22200.00 0.00 0.16716 23800.00 22400.00 0.00 0.16905 24000.00 22400.00 0.00 0.16973 23600.00 22600.00 0.00 0.17011 23800.00 22600.00 0.00 0.17195 24000.00 22600.00 0.00 0.17244 23600.00 22800.00 0.00 0.17337 23800.00 22800.00 0.00 0.17499 24000.00 22800.00 0.00 0.17520 23600.00 23000.00 0.00 0.17657 23800.00 23000.00 0.00 0.17788 23600.00 23200.00 0.00 0.17947 23800.00 23200.00 0.00 0.18038 23600.00 23400.00 0.00 0.18154 23800.00 23400.00 0.00 0.18198 23600.00 23600.00 0.00 0.18226 23600.00 23800.00 35.00 0.18228 23600.00 23800.00 40.00 0.18234 23600.00 23800.00 45.00 0.18239 23600.00 23800.00 50.00 0.18243 23600.00 23800.00 55.00 0.18246 23600.00 23800.00 60.00 0.18247 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23517.80 23762.56 63.21 0.18263 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/304.ctf NX, NY, NZ: 360 360 1 MODE : real