CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/309.mrc Output diagnostic file name Micrographs/309.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/309.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2402.000 8764.000 Mean, RMS : 4431.188 222.8805 TITLE 1: emi2mrc: 309.emi Mon Jan 20 00:33:28 2014 TITLE 2: kV 300, Mag 78000x, Def 27890, Spot 4, Int 55.12%, Obj 88.05%, Dif 73.67% TITLE 3: X -498.769, Y 536.228, Z -22.333, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.04233 15600.00 8000.00 0.00 -0.04231 15800.00 8000.00 0.00 -0.04141 16000.00 8000.00 0.00 -0.04036 16200.00 8000.00 0.00 -0.03916 16400.00 8000.00 0.00 -0.03789 16600.00 8000.00 0.00 -0.03660 16800.00 8000.00 0.00 -0.03539 17000.00 8000.00 0.00 -0.03429 17200.00 8000.00 0.00 -0.03331 17400.00 8000.00 0.00 -0.03240 17600.00 8000.00 0.00 -0.03151 17800.00 8000.00 0.00 -0.03055 18000.00 8000.00 0.00 -0.02948 18200.00 8000.00 0.00 -0.02827 18400.00 8000.00 0.00 -0.02698 18600.00 8000.00 0.00 -0.02566 18800.00 8000.00 0.00 -0.02433 19000.00 8000.00 0.00 -0.02301 19200.00 8000.00 0.00 -0.02164 19400.00 8000.00 0.00 -0.02019 19600.00 8000.00 0.00 -0.01864 19800.00 8000.00 0.00 -0.01704 20000.00 8000.00 0.00 -0.01544 20200.00 8000.00 0.00 -0.01389 20400.00 8000.00 0.00 -0.01238 20600.00 8000.00 0.00 -0.01089 20800.00 8000.00 0.00 -0.00937 21000.00 8000.00 0.00 -0.00776 21200.00 8000.00 0.00 -0.00605 21400.00 8000.00 0.00 -0.00422 21600.00 8000.00 0.00 -0.00227 21800.00 8000.00 0.00 -0.00022 22000.00 8000.00 0.00 0.00190 22200.00 8000.00 0.00 0.00406 22400.00 8000.00 0.00 0.00624 22600.00 8000.00 0.00 0.00846 22800.00 8000.00 0.00 0.01073 23000.00 8000.00 0.00 0.01308 23200.00 8000.00 0.00 0.01551 23400.00 8000.00 0.00 0.01798 23600.00 8000.00 0.00 0.02044 23800.00 8000.00 0.00 0.02291 24000.00 8000.00 0.00 0.02545 24200.00 8000.00 0.00 0.02815 24400.00 8000.00 0.00 0.03105 24600.00 8000.00 0.00 0.03411 24800.00 8000.00 0.00 0.03711 25000.00 8000.00 0.00 0.03979 25200.00 8000.00 0.00 0.04191 25400.00 8000.00 0.00 0.04331 25600.00 8000.00 0.00 0.04399 25800.00 8000.00 0.00 0.04412 25600.00 8200.00 0.00 0.04450 25800.00 8200.00 0.00 0.04466 25600.00 8400.00 0.00 0.04520 25800.00 8400.00 0.00 0.04538 25600.00 8600.00 0.00 0.04604 25800.00 8600.00 0.00 0.04622 25600.00 8800.00 0.00 0.04681 25800.00 8800.00 0.00 0.04697 25600.00 9000.00 0.00 0.04730 25800.00 9000.00 0.00 0.04741 25600.00 9200.00 0.00 0.04742 25800.00 9200.00 0.00 0.04749 25600.00 10200.00 0.00 0.04772 25800.00 10200.00 0.00 0.04787 25600.00 10400.00 0.00 0.04847 25800.00 10400.00 0.00 0.04865 25600.00 10600.00 0.00 0.04929 25800.00 10600.00 0.00 0.04951 25600.00 10800.00 0.00 0.05015 25800.00 10800.00 0.00 0.05038 25600.00 11000.00 0.00 0.05107 25800.00 11000.00 0.00 0.05129 25600.00 11200.00 0.00 0.05210 25800.00 11200.00 0.00 0.05231 25400.00 11400.00 0.00 0.05237 25600.00 11400.00 0.00 0.05319 25800.00 11400.00 0.00 0.05337 25400.00 11600.00 0.00 0.05344 25600.00 11600.00 0.00 0.05422 25800.00 11600.00 0.00 0.05437 25600.00 11800.00 0.00 0.05513 25800.00 11800.00 0.00 0.05524 25600.00 12000.00 0.00 0.05586 25800.00 12000.00 0.00 0.05593 25600.00 12200.00 0.00 0.05651 25800.00 12200.00 0.00 0.05655 25400.00 12400.00 0.00 0.05656 25600.00 12400.00 0.00 0.05723 25400.00 12600.00 0.00 0.05745 25600.00 12600.00 0.00 0.05810 25400.00 12800.00 0.00 0.05845 25600.00 12800.00 0.00 0.05908 25400.00 13000.00 0.00 0.05953 25600.00 13000.00 0.00 0.06014 25400.00 13200.00 0.00 0.06062 25600.00 13200.00 0.00 0.06119 25400.00 13400.00 0.00 0.06170 25600.00 13400.00 0.00 0.06223 25400.00 13600.00 0.00 0.06286 25600.00 13600.00 0.00 0.06336 25400.00 13800.00 0.00 0.06418 25600.00 13800.00 0.00 0.06464 25400.00 14000.00 0.00 0.06563 25600.00 14000.00 0.00 0.06606 25400.00 14200.00 0.00 0.06718 25600.00 14200.00 0.00 0.06758 25400.00 14400.00 0.00 0.06876 25600.00 14400.00 0.00 0.06914 25400.00 14600.00 0.00 0.07030 25600.00 14600.00 0.00 0.07066 25400.00 14800.00 0.00 0.07182 25600.00 14800.00 0.00 0.07215 25400.00 15000.00 0.00 0.07336 25600.00 15000.00 0.00 0.07367 25200.00 15200.00 0.00 0.07368 25400.00 15200.00 0.00 0.07489 25600.00 15200.00 0.00 0.07516 25400.00 15400.00 0.00 0.07633 25600.00 15400.00 0.00 0.07657 25400.00 15600.00 0.00 0.07767 25600.00 15600.00 0.00 0.07788 25400.00 15800.00 0.00 0.07891 25600.00 15800.00 0.00 0.07910 25400.00 16000.00 0.00 0.08014 25600.00 16000.00 0.00 0.08029 25200.00 16200.00 0.00 0.08039 25400.00 16200.00 0.00 0.08150 25600.00 16200.00 0.00 0.08162 25200.00 16400.00 0.00 0.08199 25400.00 16400.00 0.00 0.08307 25600.00 16400.00 0.00 0.08316 25200.00 16600.00 0.00 0.08376 25400.00 16600.00 0.00 0.08481 25600.00 16600.00 0.00 0.08486 25200.00 16800.00 0.00 0.08563 25400.00 16800.00 0.00 0.08666 25600.00 16800.00 0.00 0.08667 25200.00 17000.00 0.00 0.08752 25400.00 17000.00 0.00 0.08852 25200.00 17200.00 0.00 0.08938 25400.00 17200.00 0.00 0.09037 25200.00 17400.00 0.00 0.09133 25400.00 17400.00 0.00 0.09231 25200.00 17600.00 0.00 0.09344 25400.00 17600.00 0.00 0.09440 25200.00 17800.00 0.00 0.09566 25400.00 17800.00 0.00 0.09659 25200.00 18000.00 0.00 0.09792 25400.00 18000.00 0.00 0.09881 25200.00 18200.00 0.00 0.10014 25400.00 18200.00 0.00 0.10096 25200.00 18400.00 0.00 0.10219 25400.00 18400.00 0.00 0.10293 25200.00 18600.00 0.00 0.10414 25400.00 18600.00 0.00 0.10481 25200.00 18800.00 0.00 0.10614 25400.00 18800.00 0.00 0.10677 25200.00 19000.00 0.00 0.10824 25400.00 19000.00 0.00 0.10882 25200.00 19200.00 0.00 0.11039 25400.00 19200.00 0.00 0.11096 25200.00 19400.00 0.00 0.11261 25400.00 19400.00 0.00 0.11316 25200.00 19600.00 0.00 0.11479 25400.00 19600.00 0.00 0.11534 25200.00 19800.00 0.00 0.11694 25400.00 19800.00 0.00 0.11749 25200.00 20000.00 0.00 0.11920 25400.00 20000.00 0.00 0.11975 25000.00 20200.00 0.00 0.11983 25200.00 20200.00 0.00 0.12158 25400.00 20200.00 0.00 0.12211 25000.00 20400.00 0.00 0.12225 25200.00 20400.00 0.00 0.12399 25400.00 20400.00 0.00 0.12447 25000.00 20600.00 0.00 0.12467 25200.00 20600.00 0.00 0.12637 25400.00 20600.00 0.00 0.12679 25000.00 20800.00 0.00 0.12702 25200.00 20800.00 0.00 0.12867 25400.00 20800.00 0.00 0.12901 25000.00 21000.00 0.00 0.12922 25200.00 21000.00 0.00 0.13080 25400.00 21000.00 0.00 0.13105 25000.00 21200.00 0.00 0.13142 25200.00 21200.00 0.00 0.13291 25400.00 21200.00 0.00 0.13306 25000.00 21400.00 0.00 0.13374 25200.00 21400.00 0.00 0.13515 25400.00 21400.00 0.00 0.13518 25000.00 21600.00 0.00 0.13612 25200.00 21600.00 0.00 0.13742 25000.00 21800.00 0.00 0.13843 25200.00 21800.00 0.00 0.13963 25000.00 22000.00 0.00 0.14064 25200.00 22000.00 0.00 0.14174 25000.00 22200.00 0.00 0.14262 25200.00 22200.00 0.00 0.14362 25000.00 22400.00 0.00 0.14444 25200.00 22400.00 0.00 0.14537 25000.00 22600.00 0.00 0.14637 25200.00 22600.00 0.00 0.14720 25000.00 22800.00 0.00 0.14845 25200.00 22800.00 0.00 0.14917 25000.00 23000.00 0.00 0.15060 25200.00 23000.00 0.00 0.15120 25000.00 23200.00 0.00 0.15281 25200.00 23200.00 0.00 0.15328 25000.00 23400.00 0.00 0.15495 25200.00 23400.00 0.00 0.15529 25000.00 23600.00 0.00 0.15695 25200.00 23600.00 0.00 0.15716 24800.00 23800.00 0.00 0.15730 25000.00 23800.00 0.00 0.15902 25200.00 23800.00 0.00 0.15907 24800.00 24000.00 0.00 0.15968 25000.00 24000.00 0.00 0.16122 24800.00 24200.00 0.00 0.16200 25000.00 24200.00 0.00 0.16330 24800.00 24400.00 0.00 0.16414 25000.00 24400.00 0.00 0.16513 24800.00 24600.00 0.00 0.16585 25000.00 24600.00 0.00 0.16645 24800.00 24800.00 0.00 0.16667 25000.00 24800.00 0.00 0.16687 24800.00 25000.00 25.00 0.16691 24800.00 25000.00 30.00 0.16698 24800.00 25000.00 35.00 0.16703 24800.00 25000.00 40.00 0.16708 24800.00 25000.00 45.00 0.16711 24800.00 25000.00 50.00 0.16713 24800.00 25000.00 55.00 0.16714 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24743.37 24971.86 59.07 0.16720 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/309.ctf NX, NY, NZ: 360 360 1 MODE : real