CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/310.mrc Output diagnostic file name Micrographs/310.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/310.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2406.000 7716.000 Mean, RMS : 4450.090 227.8853 TITLE 1: emi2mrc: 310.emi Mon Jan 20 00:35:34 2014 TITLE 2: kV 300, Mag 78000x, Def 27890, Spot 4, Int 55.12%, Obj 88.04%, Dif 73.67% TITLE 3: X -497.082, Y 537.834, Z -22.452, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.05688 25400.00 8000.00 0.00 -0.05639 25600.00 8000.00 0.00 -0.05537 25800.00 8000.00 0.00 -0.05438 26000.00 8000.00 0.00 -0.05335 26200.00 8000.00 0.00 -0.05225 26400.00 8000.00 0.00 -0.05116 26600.00 8000.00 0.00 -0.05010 26800.00 8000.00 0.00 -0.04908 27000.00 8000.00 0.00 -0.04814 27200.00 8000.00 0.00 -0.04722 27400.00 8000.00 0.00 -0.04622 27600.00 8000.00 0.00 -0.04509 27800.00 8000.00 0.00 -0.04381 28000.00 8000.00 0.00 -0.04231 28200.00 8000.00 0.00 -0.04067 28400.00 8000.00 0.00 -0.03897 28600.00 8000.00 0.00 -0.03724 28800.00 8000.00 0.00 -0.03552 29000.00 8000.00 0.00 -0.03391 29200.00 8000.00 0.00 -0.03234 29400.00 8000.00 0.00 -0.03080 29600.00 8000.00 0.00 -0.02929 29800.00 8000.00 0.00 -0.02773 30000.00 8000.00 0.00 -0.02602 30200.00 8000.00 0.00 -0.02422 30400.00 8000.00 0.00 -0.02237 30600.00 8000.00 0.00 -0.02046 30800.00 8000.00 0.00 -0.01870 31000.00 8000.00 0.00 -0.01724 31200.00 8000.00 0.00 -0.01607 31400.00 8000.00 0.00 -0.01521 31600.00 8000.00 0.00 -0.01470 31800.00 8000.00 0.00 -0.01441 32000.00 8000.00 0.00 -0.01421 32200.00 8000.00 0.00 -0.01418 31800.00 11400.00 0.00 -0.01415 32000.00 11400.00 0.00 -0.01397 31800.00 11600.00 0.00 -0.01395 32000.00 11600.00 0.00 -0.01379 32000.00 11800.00 0.00 -0.01370 32000.00 12000.00 0.00 -0.01364 32000.00 12200.00 0.00 -0.01354 31800.00 12400.00 0.00 -0.01346 32000.00 12400.00 0.00 -0.01331 31800.00 12600.00 0.00 -0.01308 32000.00 12600.00 0.00 -0.01292 31800.00 12800.00 0.00 -0.01255 32000.00 12800.00 0.00 -0.01238 31600.00 13000.00 0.00 -0.01233 31800.00 13000.00 0.00 -0.01196 32000.00 13000.00 0.00 -0.01180 31600.00 13200.00 0.00 -0.01178 31800.00 13200.00 0.00 -0.01141 32000.00 13200.00 0.00 -0.01126 31800.00 13400.00 0.00 -0.01097 32000.00 13400.00 0.00 -0.01083 31800.00 13600.00 0.00 -0.01065 32000.00 13600.00 0.00 -0.01053 31800.00 13800.00 0.00 -0.01038 32000.00 13800.00 0.00 -0.01027 31800.00 14000.00 0.00 -0.01006 32000.00 14000.00 0.00 -0.00995 31800.00 14200.00 0.00 -0.00963 32000.00 14200.00 0.00 -0.00953 31600.00 14400.00 0.00 -0.00942 31800.00 14400.00 0.00 -0.00907 32000.00 14400.00 0.00 -0.00899 31600.00 14600.00 0.00 -0.00880 31800.00 14600.00 0.00 -0.00846 32000.00 14600.00 0.00 -0.00840 31600.00 14800.00 0.00 -0.00818 31800.00 14800.00 0.00 -0.00787 32000.00 14800.00 0.00 -0.00783 31600.00 15000.00 0.00 -0.00761 31800.00 15000.00 0.00 -0.00732 32000.00 15000.00 0.00 -0.00729 31600.00 15200.00 0.00 -0.00705 31800.00 15200.00 0.00 -0.00677 32000.00 15200.00 0.00 -0.00674 31600.00 15400.00 0.00 -0.00643 31800.00 15400.00 0.00 -0.00615 32000.00 15400.00 0.00 -0.00612 31600.00 15600.00 0.00 -0.00571 31800.00 15600.00 0.00 -0.00542 32000.00 15600.00 0.00 -0.00539 31600.00 15800.00 0.00 -0.00489 31800.00 15800.00 0.00 -0.00460 32000.00 15800.00 0.00 -0.00455 31600.00 16000.00 0.00 -0.00405 31800.00 16000.00 0.00 -0.00375 32000.00 16000.00 0.00 -0.00371 31600.00 16200.00 0.00 -0.00328 31800.00 16200.00 0.00 -0.00298 32000.00 16200.00 0.00 -0.00295 31600.00 16400.00 0.00 -0.00261 31800.00 16400.00 0.00 -0.00232 32000.00 16400.00 0.00 -0.00230 31600.00 16600.00 0.00 -0.00202 31800.00 16600.00 0.00 -0.00175 31600.00 16800.00 0.00 -0.00142 31800.00 16800.00 0.00 -0.00118 31600.00 17000.00 0.00 -0.00073 31800.00 17000.00 0.00 -0.00051 31400.00 17200.00 0.00 -0.00048 31600.00 17200.00 0.00 0.00011 31800.00 17200.00 0.00 0.00030 31400.00 17400.00 0.00 0.00053 31600.00 17400.00 0.00 0.00109 31800.00 17400.00 0.00 0.00125 31400.00 17600.00 0.00 0.00163 31600.00 17600.00 0.00 0.00217 31800.00 17600.00 0.00 0.00230 31400.00 17800.00 0.00 0.00279 31600.00 17800.00 0.00 0.00331 31800.00 17800.00 0.00 0.00342 31400.00 18000.00 0.00 0.00398 31600.00 18000.00 0.00 0.00448 31800.00 18000.00 0.00 0.00457 31400.00 18200.00 0.00 0.00521 31600.00 18200.00 0.00 0.00570 31800.00 18200.00 0.00 0.00577 31400.00 18400.00 0.00 0.00649 31600.00 18400.00 0.00 0.00696 31800.00 18400.00 0.00 0.00701 31400.00 18600.00 0.00 0.00780 31600.00 18600.00 0.00 0.00824 31800.00 18600.00 0.00 0.00827 31400.00 18800.00 0.00 0.00910 31600.00 18800.00 0.00 0.00949 31400.00 19000.00 0.00 0.01030 31600.00 19000.00 0.00 0.01064 31400.00 19200.00 0.00 0.01135 31600.00 19200.00 0.00 0.01163 31400.00 19400.00 0.00 0.01225 31600.00 19400.00 0.00 0.01249 31400.00 19600.00 0.00 0.01304 31600.00 19600.00 0.00 0.01327 31400.00 19800.00 0.00 0.01380 31600.00 19800.00 0.00 0.01404 31400.00 20000.00 0.00 0.01464 31600.00 20000.00 0.00 0.01492 31400.00 20200.00 0.00 0.01560 31600.00 20200.00 0.00 0.01593 31800.00 20200.00 0.00 0.01594 31400.00 20400.00 0.00 0.01670 31600.00 20400.00 0.00 0.01706 31800.00 20400.00 0.00 0.01712 31200.00 20600.00 0.00 0.01712 31400.00 20600.00 0.00 0.01790 31600.00 20600.00 0.00 0.01829 31800.00 20600.00 0.00 0.01836 31200.00 20800.00 0.00 0.01837 31400.00 20800.00 0.00 0.01917 31600.00 20800.00 0.00 0.01957 31800.00 20800.00 0.00 0.01964 31200.00 21000.00 0.00 0.01965 31400.00 21000.00 0.00 0.02045 31600.00 21000.00 0.00 0.02084 31800.00 21000.00 0.00 0.02091 31200.00 21200.00 0.00 0.02094 31400.00 21200.00 0.00 0.02173 31600.00 21200.00 0.00 0.02211 31800.00 21200.00 0.00 0.02215 31200.00 21400.00 0.00 0.02222 31400.00 21400.00 0.00 0.02300 31600.00 21400.00 0.00 0.02337 31800.00 21400.00 0.00 0.02339 31200.00 21600.00 0.00 0.02350 31400.00 21600.00 0.00 0.02427 31600.00 21600.00 0.00 0.02463 31800.00 21600.00 0.00 0.02464 31200.00 21800.00 0.00 0.02479 31400.00 21800.00 0.00 0.02557 31600.00 21800.00 0.00 0.02593 31800.00 21800.00 0.00 0.02595 31200.00 22000.00 0.00 0.02616 31400.00 22000.00 0.00 0.02695 31600.00 22000.00 0.00 0.02733 31800.00 22000.00 0.00 0.02737 31200.00 22200.00 0.00 0.02763 31400.00 22200.00 0.00 0.02844 31600.00 22200.00 0.00 0.02886 31800.00 22200.00 0.00 0.02892 31200.00 22400.00 0.00 0.02922 31400.00 22400.00 0.00 0.03007 31600.00 22400.00 0.00 0.03051 31800.00 22400.00 0.00 0.03059 31200.00 22600.00 0.00 0.03095 31400.00 22600.00 0.00 0.03182 31600.00 22600.00 0.00 0.03229 31800.00 22600.00 0.00 0.03238 31200.00 22800.00 0.00 0.03276 31400.00 22800.00 0.00 0.03365 31600.00 22800.00 0.00 0.03413 31800.00 22800.00 0.00 0.03423 31200.00 23000.00 0.00 0.03458 31400.00 23000.00 0.00 0.03548 31600.00 23000.00 0.00 0.03596 31800.00 23000.00 0.00 0.03606 31200.00 23200.00 0.00 0.03639 31400.00 23200.00 0.00 0.03729 31600.00 23200.00 0.00 0.03777 31800.00 23200.00 0.00 0.03785 31200.00 23400.00 0.00 0.03814 31400.00 23400.00 0.00 0.03904 31600.00 23400.00 0.00 0.03951 31800.00 23400.00 0.00 0.03956 31200.00 23600.00 0.00 0.03984 31400.00 23600.00 0.00 0.04074 31600.00 23600.00 0.00 0.04119 31800.00 23600.00 0.00 0.04122 31200.00 23800.00 0.00 0.04154 31400.00 23800.00 0.00 0.04244 31600.00 23800.00 0.00 0.04288 31800.00 23800.00 0.00 0.04290 31200.00 24000.00 0.00 0.04328 31400.00 24000.00 0.00 0.04418 31600.00 24000.00 0.00 0.04462 31800.00 24000.00 0.00 0.04463 31200.00 24200.00 0.00 0.04509 31400.00 24200.00 0.00 0.04600 31600.00 24200.00 0.00 0.04644 31200.00 24400.00 0.00 0.04702 31400.00 24400.00 0.00 0.04794 31600.00 24400.00 0.00 0.04836 31200.00 24600.00 0.00 0.04908 31400.00 24600.00 0.00 0.04998 31600.00 24600.00 0.00 0.05037 31200.00 24800.00 0.00 0.05120 31400.00 24800.00 0.00 0.05206 31600.00 24800.00 0.00 0.05240 31200.00 25000.00 0.00 0.05333 31400.00 25000.00 0.00 0.05414 31600.00 25000.00 0.00 0.05442 31200.00 25200.00 0.00 0.05542 31400.00 25200.00 0.00 0.05618 31600.00 25200.00 0.00 0.05640 31200.00 25400.00 0.00 0.05744 31400.00 25400.00 0.00 0.05815 31600.00 25400.00 0.00 0.05833 31200.00 25600.00 0.00 0.05944 31400.00 25600.00 0.00 0.06012 31600.00 25600.00 0.00 0.06025 31200.00 25800.00 0.00 0.06155 31400.00 25800.00 0.00 0.06221 31600.00 25800.00 0.00 0.06231 31000.00 26000.00 0.00 0.06237 31200.00 26000.00 0.00 0.06384 31400.00 26000.00 0.00 0.06447 31600.00 26000.00 0.00 0.06453 31000.00 26200.00 0.00 0.06484 31200.00 26200.00 0.00 0.06630 31400.00 26200.00 0.00 0.06690 31600.00 26200.00 0.00 0.06694 31000.00 26400.00 0.00 0.06747 31200.00 26400.00 0.00 0.06893 31400.00 26400.00 0.00 0.06950 31600.00 26400.00 0.00 0.06950 31000.00 26600.00 0.00 0.07011 31200.00 26600.00 0.00 0.07155 31400.00 26600.00 0.00 0.07209 31000.00 26800.00 0.00 0.07261 31200.00 26800.00 0.00 0.07404 31400.00 26800.00 0.00 0.07454 31000.00 27000.00 0.00 0.07505 31200.00 27000.00 0.00 0.07648 31400.00 27000.00 0.00 0.07695 31000.00 27200.00 0.00 0.07759 31200.00 27200.00 0.00 0.07901 31400.00 27200.00 0.00 0.07946 31000.00 27400.00 0.00 0.08036 31200.00 27400.00 0.00 0.08178 31400.00 27400.00 0.00 0.08221 31000.00 27600.00 0.00 0.08356 31200.00 27600.00 0.00 0.08498 31400.00 27600.00 0.00 0.08537 31000.00 27800.00 0.00 0.08720 31200.00 27800.00 0.00 0.08860 31400.00 27800.00 0.00 0.08893 31000.00 28000.00 0.00 0.09101 31200.00 28000.00 0.00 0.09235 31400.00 28000.00 0.00 0.09259 31000.00 28200.00 0.00 0.09481 31200.00 28200.00 0.00 0.09607 31400.00 28200.00 0.00 0.09617 30800.00 28400.00 0.00 0.09617 31000.00 28400.00 0.00 0.09854 31200.00 28400.00 0.00 0.09967 30800.00 28600.00 0.00 0.09982 31000.00 28600.00 0.00 0.10209 31200.00 28600.00 0.00 0.10308 30800.00 28800.00 0.00 0.10343 31000.00 28800.00 0.00 0.10558 31200.00 28800.00 0.00 0.10641 30800.00 29000.00 0.00 0.10717 31000.00 29000.00 0.00 0.10920 31200.00 29000.00 0.00 0.10987 30800.00 29200.00 0.00 0.11092 31000.00 29200.00 0.00 0.11283 31200.00 29200.00 0.00 0.11332 30800.00 29400.00 0.00 0.11460 31000.00 29400.00 0.00 0.11637 31200.00 29400.00 0.00 0.11666 30800.00 29600.00 0.00 0.11838 31000.00 29600.00 0.00 0.11999 31200.00 29600.00 0.00 0.12006 30800.00 29800.00 0.00 0.12222 31000.00 29800.00 0.00 0.12364 30600.00 30000.00 0.00 0.12368 30800.00 30000.00 0.00 0.12605 31000.00 30000.00 0.00 0.12723 30600.00 30200.00 0.00 0.12788 30800.00 30200.00 0.00 0.13002 31000.00 30200.00 0.00 0.13089 30600.00 30400.00 0.00 0.13185 30800.00 30400.00 0.00 0.13365 31000.00 30400.00 0.00 0.13411 30600.00 30600.00 0.00 0.13457 30800.00 30600.00 0.00 0.13593 30800.00 30800.00 0.00 0.13643 30600.00 31000.00 45.00 0.13652 30600.00 31000.00 50.00 0.13661 30600.00 31000.00 55.00 0.13667 30600.00 31000.00 60.00 0.13668 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 30678.71 30924.97 58.24 0.13685 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/310.ctf NX, NY, NZ: 360 360 1 MODE : real