CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/312.mrc Output diagnostic file name Micrographs/312.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/312.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2462.000 7988.000 Mean, RMS : 4446.538 226.7435 TITLE 1: emi2mrc: 312.emi Mon Jan 20 00:36:51 2014 TITLE 2: kV 300, Mag 78000x, Def 27890, Spot 4, Int 55.12%, Obj 88.04%, Dif 73.67% TITLE 3: X -493.526, Y 542.181, Z -22.452, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.06327 24200.00 8000.00 0.00 -0.06250 24400.00 8000.00 0.00 -0.06148 24600.00 8000.00 0.00 -0.06042 24800.00 8000.00 0.00 -0.05932 25000.00 8000.00 0.00 -0.05822 25200.00 8000.00 0.00 -0.05718 25400.00 8000.00 0.00 -0.05616 25600.00 8000.00 0.00 -0.05512 25800.00 8000.00 0.00 -0.05402 26000.00 8000.00 0.00 -0.05280 26200.00 8000.00 0.00 -0.05142 26400.00 8000.00 0.00 -0.05002 26600.00 8000.00 0.00 -0.04868 26800.00 8000.00 0.00 -0.04744 27000.00 8000.00 0.00 -0.04634 27200.00 8000.00 0.00 -0.04531 27400.00 8000.00 0.00 -0.04420 27600.00 8000.00 0.00 -0.04298 27800.00 8000.00 0.00 -0.04159 28000.00 8000.00 0.00 -0.04001 28200.00 8000.00 0.00 -0.03829 28400.00 8000.00 0.00 -0.03651 28600.00 8000.00 0.00 -0.03462 28800.00 8000.00 0.00 -0.03263 29000.00 8000.00 0.00 -0.03059 29200.00 8000.00 0.00 -0.02845 29400.00 8000.00 0.00 -0.02622 29600.00 8000.00 0.00 -0.02398 29800.00 8000.00 0.00 -0.02169 30000.00 8000.00 0.00 -0.01936 30200.00 8000.00 0.00 -0.01709 30400.00 8000.00 0.00 -0.01498 30600.00 8000.00 0.00 -0.01315 30800.00 8000.00 0.00 -0.01180 31000.00 8000.00 0.00 -0.01103 31200.00 8000.00 0.00 -0.01078 31000.00 11400.00 0.00 -0.01053 31200.00 11400.00 0.00 -0.01050 31000.00 11600.00 0.00 -0.01010 31200.00 11600.00 0.00 -0.01010 31000.00 11800.00 0.00 -0.00979 31000.00 12000.00 0.00 -0.00960 31000.00 12200.00 0.00 -0.00954 31000.00 13400.00 0.00 -0.00941 31000.00 13600.00 0.00 -0.00913 31000.00 13800.00 0.00 -0.00873 31200.00 13800.00 0.00 -0.00868 31000.00 14000.00 0.00 -0.00823 31200.00 14000.00 0.00 -0.00811 31000.00 14200.00 0.00 -0.00763 31200.00 14200.00 0.00 -0.00745 31000.00 14400.00 0.00 -0.00695 31200.00 14400.00 0.00 -0.00673 31400.00 14400.00 0.00 -0.00669 31000.00 14600.00 0.00 -0.00623 31200.00 14600.00 0.00 -0.00595 31400.00 14600.00 0.00 -0.00589 31000.00 14800.00 0.00 -0.00546 31200.00 14800.00 0.00 -0.00516 31400.00 14800.00 0.00 -0.00508 31000.00 15000.00 0.00 -0.00468 31200.00 15000.00 0.00 -0.00435 31400.00 15000.00 0.00 -0.00428 31000.00 15200.00 0.00 -0.00388 31200.00 15200.00 0.00 -0.00354 31400.00 15200.00 0.00 -0.00348 31000.00 15400.00 0.00 -0.00305 31200.00 15400.00 0.00 -0.00272 31400.00 15400.00 0.00 -0.00268 31000.00 15600.00 0.00 -0.00220 31200.00 15600.00 0.00 -0.00188 31400.00 15600.00 0.00 -0.00187 31000.00 15800.00 0.00 -0.00132 31200.00 15800.00 0.00 -0.00102 31000.00 16000.00 0.00 -0.00040 31200.00 16000.00 0.00 -0.00012 31000.00 16200.00 0.00 0.00055 31200.00 16200.00 0.00 0.00083 31000.00 16400.00 0.00 0.00154 31200.00 16400.00 0.00 0.00181 31000.00 16600.00 0.00 0.00258 31200.00 16600.00 0.00 0.00285 30800.00 16800.00 0.00 0.00287 31000.00 16800.00 0.00 0.00367 31200.00 16800.00 0.00 0.00396 30800.00 17000.00 0.00 0.00403 31000.00 17000.00 0.00 0.00484 31200.00 17000.00 0.00 0.00514 30800.00 17200.00 0.00 0.00530 31000.00 17200.00 0.00 0.00612 31200.00 17200.00 0.00 0.00642 30800.00 17400.00 0.00 0.00666 31000.00 17400.00 0.00 0.00748 31200.00 17400.00 0.00 0.00777 30800.00 17600.00 0.00 0.00808 31000.00 17600.00 0.00 0.00889 31200.00 17600.00 0.00 0.00917 30800.00 17800.00 0.00 0.00950 31000.00 17800.00 0.00 0.01029 31200.00 17800.00 0.00 0.01055 30800.00 18000.00 0.00 0.01086 31000.00 18000.00 0.00 0.01163 31200.00 18000.00 0.00 0.01186 30800.00 18200.00 0.00 0.01215 31000.00 18200.00 0.00 0.01288 31200.00 18200.00 0.00 0.01309 30800.00 18400.00 0.00 0.01337 31000.00 18400.00 0.00 0.01409 31200.00 18400.00 0.00 0.01427 30800.00 18600.00 0.00 0.01459 31000.00 18600.00 0.00 0.01529 31200.00 18600.00 0.00 0.01545 30800.00 18800.00 0.00 0.01586 31000.00 18800.00 0.00 0.01655 31200.00 18800.00 0.00 0.01670 30800.00 19000.00 0.00 0.01720 31000.00 19000.00 0.00 0.01788 31200.00 19000.00 0.00 0.01802 30800.00 19200.00 0.00 0.01857 31000.00 19200.00 0.00 0.01925 31200.00 19200.00 0.00 0.01937 30800.00 19400.00 0.00 0.01996 31000.00 19400.00 0.00 0.02063 31200.00 19400.00 0.00 0.02073 30800.00 19600.00 0.00 0.02131 31000.00 19600.00 0.00 0.02198 31200.00 19600.00 0.00 0.02204 30800.00 19800.00 0.00 0.02263 31000.00 19800.00 0.00 0.02328 31200.00 19800.00 0.00 0.02330 30800.00 20000.00 0.00 0.02395 31000.00 20000.00 0.00 0.02456 30800.00 20200.00 0.00 0.02528 31000.00 20200.00 0.00 0.02586 30800.00 20400.00 0.00 0.02662 31000.00 20400.00 0.00 0.02716 30800.00 20600.00 0.00 0.02796 31000.00 20600.00 0.00 0.02844 30800.00 20800.00 0.00 0.02928 31000.00 20800.00 0.00 0.02972 30800.00 21000.00 0.00 0.03059 31000.00 21000.00 0.00 0.03098 30800.00 21200.00 0.00 0.03190 31000.00 21200.00 0.00 0.03226 30800.00 21400.00 0.00 0.03327 31000.00 21400.00 0.00 0.03361 30600.00 21600.00 0.00 0.03364 30800.00 21600.00 0.00 0.03468 31000.00 21600.00 0.00 0.03503 30600.00 21800.00 0.00 0.03509 30800.00 21800.00 0.00 0.03614 31000.00 21800.00 0.00 0.03651 30600.00 22000.00 0.00 0.03658 30800.00 22000.00 0.00 0.03765 31000.00 22000.00 0.00 0.03805 30600.00 22200.00 0.00 0.03813 30800.00 22200.00 0.00 0.03923 31000.00 22200.00 0.00 0.03965 30600.00 22400.00 0.00 0.03977 30800.00 22400.00 0.00 0.04089 31000.00 22400.00 0.00 0.04133 30600.00 22600.00 0.00 0.04151 30800.00 22600.00 0.00 0.04264 31000.00 22600.00 0.00 0.04309 30600.00 22800.00 0.00 0.04330 30800.00 22800.00 0.00 0.04443 31000.00 22800.00 0.00 0.04487 30600.00 23000.00 0.00 0.04505 30800.00 23000.00 0.00 0.04617 31000.00 23000.00 0.00 0.04660 30600.00 23200.00 0.00 0.04672 30800.00 23200.00 0.00 0.04784 31000.00 23200.00 0.00 0.04825 30600.00 23400.00 0.00 0.04829 30800.00 23400.00 0.00 0.04941 31000.00 23400.00 0.00 0.04982 30800.00 23600.00 0.00 0.05094 31000.00 23600.00 0.00 0.05136 30600.00 23800.00 0.00 0.05139 30800.00 23800.00 0.00 0.05255 31000.00 23800.00 0.00 0.05300 30600.00 24000.00 0.00 0.05311 30800.00 24000.00 0.00 0.05431 31000.00 24000.00 0.00 0.05478 30600.00 24200.00 0.00 0.05496 30800.00 24200.00 0.00 0.05619 31000.00 24200.00 0.00 0.05666 30600.00 24400.00 0.00 0.05691 30800.00 24400.00 0.00 0.05815 31000.00 24400.00 0.00 0.05863 30600.00 24600.00 0.00 0.05890 30800.00 24600.00 0.00 0.06016 31000.00 24600.00 0.00 0.06061 30600.00 24800.00 0.00 0.06089 30800.00 24800.00 0.00 0.06213 31000.00 24800.00 0.00 0.06254 30600.00 25000.00 0.00 0.06289 30800.00 25000.00 0.00 0.06411 31000.00 25000.00 0.00 0.06447 30600.00 25200.00 0.00 0.06497 30800.00 25200.00 0.00 0.06614 31000.00 25200.00 0.00 0.06644 30600.00 25400.00 0.00 0.06707 30800.00 25400.00 0.00 0.06820 31000.00 25400.00 0.00 0.06845 30600.00 25600.00 0.00 0.06920 30800.00 25600.00 0.00 0.07030 31000.00 25600.00 0.00 0.07050 30600.00 25800.00 0.00 0.07141 30800.00 25800.00 0.00 0.07247 31000.00 25800.00 0.00 0.07262 30600.00 26000.00 0.00 0.07364 30800.00 26000.00 0.00 0.07467 31000.00 26000.00 0.00 0.07477 30600.00 26200.00 0.00 0.07594 30800.00 26200.00 0.00 0.07691 31000.00 26200.00 0.00 0.07694 30600.00 26400.00 0.00 0.07839 30800.00 26400.00 0.00 0.07930 30600.00 26600.00 0.00 0.08095 30800.00 26600.00 0.00 0.08178 30600.00 26800.00 0.00 0.08351 30800.00 26800.00 0.00 0.08426 30400.00 27000.00 0.00 0.08427 30600.00 27000.00 0.00 0.08607 30800.00 27000.00 0.00 0.08675 30400.00 27200.00 0.00 0.08684 30600.00 27200.00 0.00 0.08860 30800.00 27200.00 0.00 0.08920 30400.00 27400.00 0.00 0.08935 30600.00 27400.00 0.00 0.09105 30800.00 27400.00 0.00 0.09158 30400.00 27600.00 0.00 0.09194 30600.00 27600.00 0.00 0.09358 30800.00 27600.00 0.00 0.09403 30400.00 27800.00 0.00 0.09469 30600.00 27800.00 0.00 0.09627 30800.00 27800.00 0.00 0.09662 30400.00 28000.00 0.00 0.09748 30600.00 28000.00 0.00 0.09898 30800.00 28000.00 0.00 0.09922 30400.00 28200.00 0.00 0.10027 30600.00 28200.00 0.00 0.10167 30800.00 28200.00 0.00 0.10178 30400.00 28400.00 0.00 0.10303 30600.00 28400.00 0.00 0.10432 30400.00 28600.00 0.00 0.10559 30600.00 28600.00 0.00 0.10675 30400.00 28800.00 0.00 0.10801 30600.00 28800.00 0.00 0.10900 30400.00 29000.00 0.00 0.11043 30600.00 29000.00 0.00 0.11122 30400.00 29200.00 0.00 0.11267 30600.00 29200.00 0.00 0.11322 30400.00 29400.00 0.00 0.11458 30600.00 29400.00 0.00 0.11488 30400.00 29600.00 0.00 0.11622 30600.00 29600.00 0.00 0.11624 30400.00 29800.00 0.00 0.11734 30400.00 30000.00 0.00 0.11781 30400.00 30200.00 0.00 0.11792 30200.00 30400.00 35.00 0.11795 30200.00 30400.00 40.00 0.11804 30200.00 30400.00 45.00 0.11811 30200.00 30400.00 50.00 0.11816 30200.00 30400.00 55.00 0.11819 30200.00 30400.00 60.00 0.11821 30000.00 30400.00 65.00 0.11824 30000.00 30400.00 70.00 0.11824 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 30040.47 30477.40 63.07 0.11837 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/312.ctf NX, NY, NZ: 360 360 1 MODE : real