CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/314.mrc Output diagnostic file name Micrographs/314.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/314.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2260.000 8176.000 Mean, RMS : 4405.136 225.4594 TITLE 1: emi2mrc: 314.emi Mon Jan 20 00:40:04 2014 TITLE 2: kV 300, Mag 78000x, Def 29040, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -488.648, Y 548.314, Z -23.326, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03561 14200.00 8000.00 0.00 -0.03533 14400.00 8000.00 0.00 -0.03491 14600.00 8000.00 0.00 -0.03434 14800.00 8000.00 0.00 -0.03361 15000.00 8000.00 0.00 -0.03277 15200.00 8000.00 0.00 -0.03188 15400.00 8000.00 0.00 -0.03097 15600.00 8000.00 0.00 -0.03004 15800.00 8000.00 0.00 -0.02905 16000.00 8000.00 0.00 -0.02797 16200.00 8000.00 0.00 -0.02682 16400.00 8000.00 0.00 -0.02560 16600.00 8000.00 0.00 -0.02432 16800.00 8000.00 0.00 -0.02299 17000.00 8000.00 0.00 -0.02165 17200.00 8000.00 0.00 -0.02032 17400.00 8000.00 0.00 -0.01899 17600.00 8000.00 0.00 -0.01767 17800.00 8000.00 0.00 -0.01628 18000.00 8000.00 0.00 -0.01476 18200.00 8000.00 0.00 -0.01309 18400.00 8000.00 0.00 -0.01132 18600.00 8000.00 0.00 -0.00953 18800.00 8000.00 0.00 -0.00781 19000.00 8000.00 0.00 -0.00616 19200.00 8000.00 0.00 -0.00451 19400.00 8000.00 0.00 -0.00278 19600.00 8000.00 0.00 -0.00090 19800.00 8000.00 0.00 0.00108 20000.00 8000.00 0.00 0.00308 20200.00 8000.00 0.00 0.00497 20400.00 8000.00 0.00 0.00670 20600.00 8000.00 0.00 0.00830 20800.00 8000.00 0.00 0.00984 21000.00 8000.00 0.00 0.01144 21200.00 8000.00 0.00 0.01317 21400.00 8000.00 0.00 0.01506 21600.00 8000.00 0.00 0.01710 21800.00 8000.00 0.00 0.01922 22000.00 8000.00 0.00 0.02142 22200.00 8000.00 0.00 0.02367 22400.00 8000.00 0.00 0.02599 22600.00 8000.00 0.00 0.02845 22800.00 8000.00 0.00 0.03110 23000.00 8000.00 0.00 0.03393 23200.00 8000.00 0.00 0.03691 23400.00 8000.00 0.00 0.03989 23600.00 8000.00 0.00 0.04269 23800.00 8000.00 0.00 0.04513 24000.00 8000.00 0.00 0.04710 24200.00 8000.00 0.00 0.04853 24400.00 8000.00 0.00 0.04945 24600.00 8000.00 0.00 0.04994 24800.00 8000.00 0.00 0.05008 24600.00 8200.00 0.00 0.05030 24800.00 8200.00 0.00 0.05044 24600.00 8400.00 0.00 0.05074 24800.00 8400.00 0.00 0.05090 24600.00 8600.00 0.00 0.05131 24800.00 8600.00 0.00 0.05149 24600.00 8800.00 0.00 0.05194 24800.00 8800.00 0.00 0.05214 24600.00 9000.00 0.00 0.05256 24800.00 9000.00 0.00 0.05277 24600.00 9200.00 0.00 0.05315 24800.00 9200.00 0.00 0.05337 24600.00 9400.00 0.00 0.05374 24800.00 9400.00 0.00 0.05397 24600.00 9600.00 0.00 0.05441 24800.00 9600.00 0.00 0.05462 24600.00 9800.00 0.00 0.05523 24800.00 9800.00 0.00 0.05543 24400.00 10000.00 0.00 0.05557 24600.00 10000.00 0.00 0.05619 24800.00 10000.00 0.00 0.05637 24400.00 10200.00 0.00 0.05660 24600.00 10200.00 0.00 0.05720 24800.00 10200.00 0.00 0.05735 24400.00 10400.00 0.00 0.05762 24600.00 10400.00 0.00 0.05819 24800.00 10400.00 0.00 0.05831 24400.00 10600.00 0.00 0.05860 24600.00 10600.00 0.00 0.05915 24800.00 10600.00 0.00 0.05924 24400.00 10800.00 0.00 0.05958 24600.00 10800.00 0.00 0.06011 24800.00 10800.00 0.00 0.06018 24400.00 11000.00 0.00 0.06063 24600.00 11000.00 0.00 0.06115 24800.00 11000.00 0.00 0.06119 24400.00 11200.00 0.00 0.06180 24600.00 11200.00 0.00 0.06229 24800.00 11200.00 0.00 0.06232 24400.00 11400.00 0.00 0.06296 24600.00 11400.00 0.00 0.06343 24800.00 11400.00 0.00 0.06344 24400.00 11600.00 0.00 0.06399 24600.00 11600.00 0.00 0.06445 24400.00 11800.00 0.00 0.06487 24600.00 11800.00 0.00 0.06532 24400.00 12000.00 0.00 0.06562 24600.00 12000.00 0.00 0.06606 24400.00 12200.00 0.00 0.06638 24600.00 12200.00 0.00 0.06681 24400.00 12400.00 0.00 0.06731 24600.00 12400.00 0.00 0.06774 24400.00 12600.00 0.00 0.06844 24600.00 12600.00 0.00 0.06885 24400.00 12800.00 0.00 0.06966 24600.00 12800.00 0.00 0.07005 24400.00 13000.00 0.00 0.07089 24600.00 13000.00 0.00 0.07126 24400.00 13200.00 0.00 0.07205 24600.00 13200.00 0.00 0.07239 24400.00 13400.00 0.00 0.07313 24600.00 13400.00 0.00 0.07345 24400.00 13600.00 0.00 0.07426 24600.00 13600.00 0.00 0.07457 24200.00 13800.00 0.00 0.07461 24400.00 13800.00 0.00 0.07552 24600.00 13800.00 0.00 0.07581 24200.00 14000.00 0.00 0.07594 24400.00 14000.00 0.00 0.07685 24600.00 14000.00 0.00 0.07715 24200.00 14200.00 0.00 0.07730 24400.00 14200.00 0.00 0.07821 24600.00 14200.00 0.00 0.07850 24200.00 14400.00 0.00 0.07864 24400.00 14400.00 0.00 0.07955 24600.00 14400.00 0.00 0.07983 24200.00 14600.00 0.00 0.07997 24400.00 14600.00 0.00 0.08088 24600.00 14600.00 0.00 0.08115 24200.00 14800.00 0.00 0.08142 24400.00 14800.00 0.00 0.08233 24600.00 14800.00 0.00 0.08259 24200.00 15000.00 0.00 0.08312 24400.00 15000.00 0.00 0.08403 24600.00 15000.00 0.00 0.08430 24200.00 15200.00 0.00 0.08508 24400.00 15200.00 0.00 0.08601 24600.00 15200.00 0.00 0.08628 24200.00 15400.00 0.00 0.08720 24400.00 15400.00 0.00 0.08813 24600.00 15400.00 0.00 0.08839 24200.00 15600.00 0.00 0.08934 24400.00 15600.00 0.00 0.09026 24600.00 15600.00 0.00 0.09051 24200.00 15800.00 0.00 0.09136 24400.00 15800.00 0.00 0.09228 24600.00 15800.00 0.00 0.09251 24200.00 16000.00 0.00 0.09328 24400.00 16000.00 0.00 0.09418 24600.00 16000.00 0.00 0.09439 24200.00 16200.00 0.00 0.09525 24400.00 16200.00 0.00 0.09614 24600.00 16200.00 0.00 0.09632 24200.00 16400.00 0.00 0.09735 24400.00 16400.00 0.00 0.09823 24600.00 16400.00 0.00 0.09838 24200.00 16600.00 0.00 0.09958 24400.00 16600.00 0.00 0.10044 24600.00 16600.00 0.00 0.10056 24200.00 16800.00 0.00 0.10188 24400.00 16800.00 0.00 0.10273 24600.00 16800.00 0.00 0.10282 24200.00 17000.00 0.00 0.10416 24400.00 17000.00 0.00 0.10498 24600.00 17000.00 0.00 0.10503 24200.00 17200.00 0.00 0.10632 24400.00 17200.00 0.00 0.10710 24600.00 17200.00 0.00 0.10711 24200.00 17400.00 0.00 0.10845 24400.00 17400.00 0.00 0.10920 24200.00 17600.00 0.00 0.11065 24400.00 17600.00 0.00 0.11137 24200.00 17800.00 0.00 0.11292 24400.00 17800.00 0.00 0.11359 24000.00 18000.00 0.00 0.11362 24200.00 18000.00 0.00 0.11524 24400.00 18000.00 0.00 0.11586 24000.00 18200.00 0.00 0.11598 24200.00 18200.00 0.00 0.11757 24400.00 18200.00 0.00 0.11815 24000.00 18400.00 0.00 0.11826 24200.00 18400.00 0.00 0.11982 24400.00 18400.00 0.00 0.12035 24000.00 18600.00 0.00 0.12049 24200.00 18600.00 0.00 0.12202 24400.00 18600.00 0.00 0.12250 24000.00 18800.00 0.00 0.12280 24200.00 18800.00 0.00 0.12428 24400.00 18800.00 0.00 0.12470 24000.00 19000.00 0.00 0.12514 24200.00 19000.00 0.00 0.12658 24400.00 19000.00 0.00 0.12694 24000.00 19200.00 0.00 0.12749 24200.00 19200.00 0.00 0.12888 24400.00 19200.00 0.00 0.12918 24000.00 19400.00 0.00 0.12987 24200.00 19400.00 0.00 0.13122 24400.00 19400.00 0.00 0.13145 24000.00 19600.00 0.00 0.13222 24200.00 19600.00 0.00 0.13352 24400.00 19600.00 0.00 0.13370 24000.00 19800.00 0.00 0.13451 24200.00 19800.00 0.00 0.13578 24400.00 19800.00 0.00 0.13591 24000.00 20000.00 0.00 0.13691 24200.00 20000.00 0.00 0.13815 24400.00 20000.00 0.00 0.13823 24000.00 20200.00 0.00 0.13947 24200.00 20200.00 0.00 0.14068 24400.00 20200.00 0.00 0.14072 24000.00 20400.00 0.00 0.14212 24200.00 20400.00 0.00 0.14330 24000.00 20600.00 0.00 0.14487 24200.00 20600.00 0.00 0.14603 24000.00 20800.00 0.00 0.14773 24200.00 20800.00 0.00 0.14887 24000.00 21000.00 0.00 0.15063 24200.00 21000.00 0.00 0.15174 24000.00 21200.00 0.00 0.15365 24200.00 21200.00 0.00 0.15469 24000.00 21400.00 0.00 0.15685 24200.00 21400.00 0.00 0.15780 23800.00 21600.00 0.00 0.15781 24000.00 21600.00 0.00 0.16006 24200.00 21600.00 0.00 0.16088 23800.00 21800.00 0.00 0.16100 24000.00 21800.00 0.00 0.16315 24200.00 21800.00 0.00 0.16381 23800.00 22000.00 0.00 0.16408 24000.00 22000.00 0.00 0.16611 24200.00 22000.00 0.00 0.16662 23800.00 22200.00 0.00 0.16698 24000.00 22200.00 0.00 0.16890 24200.00 22200.00 0.00 0.16924 23800.00 22400.00 0.00 0.16988 24000.00 22400.00 0.00 0.17167 24200.00 22400.00 0.00 0.17182 23800.00 22600.00 0.00 0.17308 24000.00 22600.00 0.00 0.17468 23800.00 22800.00 0.00 0.17650 24000.00 22800.00 0.00 0.17785 23800.00 23000.00 0.00 0.17986 24000.00 23000.00 0.00 0.18089 23800.00 23200.00 0.00 0.18296 24000.00 23200.00 0.00 0.18361 23800.00 23400.00 0.00 0.18535 24000.00 23400.00 0.00 0.18558 23800.00 23600.00 0.00 0.18666 23800.00 23800.00 0.00 0.18704 23800.00 24000.00 20.00 0.18705 23800.00 24000.00 25.00 0.18711 23800.00 24000.00 30.00 0.18716 23800.00 24000.00 35.00 0.18720 23800.00 24000.00 40.00 0.18721 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23712.28 23975.51 48.36 0.18737 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/314.ctf NX, NY, NZ: 360 360 1 MODE : real