CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/323.mrc Output diagnostic file name Micrographs/323.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/323.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2484.000 8234.000 Mean, RMS : 4407.018 230.1699 TITLE 1: emi2mrc: 323.emi Mon Jan 20 00:47:21 2014 TITLE 2: kV 300, Mag 78000x, Def 29040, Spot 4, Int 55.12%, Obj 88.07%, Dif 73.67% TITLE 3: X -489.711, Y 551.628, Z -23.326, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03183 16600.00 8000.00 0.00 -0.03129 16800.00 8000.00 0.00 -0.03032 17000.00 8000.00 0.00 -0.02923 17200.00 8000.00 0.00 -0.02805 17400.00 8000.00 0.00 -0.02683 17600.00 8000.00 0.00 -0.02561 17800.00 8000.00 0.00 -0.02442 18000.00 8000.00 0.00 -0.02330 18200.00 8000.00 0.00 -0.02224 18400.00 8000.00 0.00 -0.02125 18600.00 8000.00 0.00 -0.02026 18800.00 8000.00 0.00 -0.01923 19000.00 8000.00 0.00 -0.01809 19200.00 8000.00 0.00 -0.01683 19400.00 8000.00 0.00 -0.01545 19600.00 8000.00 0.00 -0.01402 19800.00 8000.00 0.00 -0.01260 20000.00 8000.00 0.00 -0.01121 20200.00 8000.00 0.00 -0.00989 20400.00 8000.00 0.00 -0.00858 20600.00 8000.00 0.00 -0.00727 20800.00 8000.00 0.00 -0.00591 21000.00 8000.00 0.00 -0.00451 21200.00 8000.00 0.00 -0.00305 21400.00 8000.00 0.00 -0.00155 21600.00 8000.00 0.00 0.00002 21800.00 8000.00 0.00 0.00166 22000.00 8000.00 0.00 0.00337 22200.00 8000.00 0.00 0.00511 22400.00 8000.00 0.00 0.00688 22600.00 8000.00 0.00 0.00868 22800.00 8000.00 0.00 0.01052 23000.00 8000.00 0.00 0.01244 23200.00 8000.00 0.00 0.01450 23400.00 8000.00 0.00 0.01669 23600.00 8000.00 0.00 0.01900 23800.00 8000.00 0.00 0.02143 24000.00 8000.00 0.00 0.02396 24200.00 8000.00 0.00 0.02659 24400.00 8000.00 0.00 0.02929 24600.00 8000.00 0.00 0.03203 24800.00 8000.00 0.00 0.03468 25000.00 8000.00 0.00 0.03709 25200.00 8000.00 0.00 0.03912 25400.00 8000.00 0.00 0.04062 25600.00 8000.00 0.00 0.04156 25800.00 8000.00 0.00 0.04204 26000.00 8000.00 0.00 0.04217 25800.00 8200.00 0.00 0.04241 26000.00 8200.00 0.00 0.04254 25800.00 8400.00 0.00 0.04284 26000.00 8400.00 0.00 0.04298 25800.00 8600.00 0.00 0.04335 26000.00 8600.00 0.00 0.04349 25800.00 8800.00 0.00 0.04388 26000.00 8800.00 0.00 0.04401 25800.00 9000.00 0.00 0.04435 26000.00 9000.00 0.00 0.04448 25800.00 9200.00 0.00 0.04474 26000.00 9200.00 0.00 0.04486 25800.00 9400.00 0.00 0.04504 26000.00 9400.00 0.00 0.04515 25800.00 9600.00 0.00 0.04526 26000.00 9600.00 0.00 0.04537 25800.00 9800.00 0.00 0.04547 26000.00 9800.00 0.00 0.04559 25800.00 10000.00 0.00 0.04572 26000.00 10000.00 0.00 0.04586 25800.00 10200.00 0.00 0.04602 26000.00 10200.00 0.00 0.04618 25800.00 10400.00 0.00 0.04638 26000.00 10400.00 0.00 0.04657 25800.00 10600.00 0.00 0.04682 26000.00 10600.00 0.00 0.04705 26200.00 10600.00 0.00 0.04705 25800.00 10800.00 0.00 0.04735 26000.00 10800.00 0.00 0.04760 26200.00 10800.00 0.00 0.04760 25800.00 11000.00 0.00 0.04799 26000.00 11000.00 0.00 0.04824 25800.00 11200.00 0.00 0.04875 26000.00 11200.00 0.00 0.04899 25800.00 11400.00 0.00 0.04956 26000.00 11400.00 0.00 0.04979 25800.00 11600.00 0.00 0.05039 26000.00 11600.00 0.00 0.05058 25600.00 11800.00 0.00 0.05060 25800.00 11800.00 0.00 0.05119 26000.00 11800.00 0.00 0.05134 25600.00 12000.00 0.00 0.05138 25800.00 12000.00 0.00 0.05195 26000.00 12000.00 0.00 0.05207 25600.00 12200.00 0.00 0.05216 25800.00 12200.00 0.00 0.05270 26000.00 12200.00 0.00 0.05280 25600.00 12400.00 0.00 0.05300 25800.00 12400.00 0.00 0.05353 26000.00 12400.00 0.00 0.05361 25600.00 12600.00 0.00 0.05390 25800.00 12600.00 0.00 0.05442 26000.00 12600.00 0.00 0.05449 25600.00 12800.00 0.00 0.05484 25800.00 12800.00 0.00 0.05536 26000.00 12800.00 0.00 0.05543 25600.00 13000.00 0.00 0.05581 25800.00 13000.00 0.00 0.05633 26000.00 13000.00 0.00 0.05641 25600.00 13200.00 0.00 0.05680 25800.00 13200.00 0.00 0.05733 26000.00 13200.00 0.00 0.05741 25600.00 13400.00 0.00 0.05779 25800.00 13400.00 0.00 0.05833 26000.00 13400.00 0.00 0.05842 25600.00 13600.00 0.00 0.05885 25800.00 13600.00 0.00 0.05939 26000.00 13600.00 0.00 0.05947 25600.00 13800.00 0.00 0.05997 25800.00 13800.00 0.00 0.06050 26000.00 13800.00 0.00 0.06058 25600.00 14000.00 0.00 0.06108 25800.00 14000.00 0.00 0.06161 26000.00 14000.00 0.00 0.06168 25600.00 14200.00 0.00 0.06215 25800.00 14200.00 0.00 0.06267 26000.00 14200.00 0.00 0.06273 25600.00 14400.00 0.00 0.06317 25800.00 14400.00 0.00 0.06369 26000.00 14400.00 0.00 0.06374 25600.00 14600.00 0.00 0.06416 25800.00 14600.00 0.00 0.06467 26000.00 14600.00 0.00 0.06471 25600.00 14800.00 0.00 0.06521 25800.00 14800.00 0.00 0.06571 26000.00 14800.00 0.00 0.06575 25600.00 15000.00 0.00 0.06641 25800.00 15000.00 0.00 0.06692 26000.00 15000.00 0.00 0.06697 25600.00 15200.00 0.00 0.06780 25800.00 15200.00 0.00 0.06831 26000.00 15200.00 0.00 0.06837 25600.00 15400.00 0.00 0.06931 25800.00 15400.00 0.00 0.06983 26000.00 15400.00 0.00 0.06989 25600.00 15600.00 0.00 0.07091 25800.00 15600.00 0.00 0.07143 26000.00 15600.00 0.00 0.07149 25600.00 15800.00 0.00 0.07251 25800.00 15800.00 0.00 0.07304 26000.00 15800.00 0.00 0.07309 25600.00 16000.00 0.00 0.07412 25800.00 16000.00 0.00 0.07464 26000.00 16000.00 0.00 0.07469 25600.00 16200.00 0.00 0.07579 25800.00 16200.00 0.00 0.07631 26000.00 16200.00 0.00 0.07635 25400.00 16400.00 0.00 0.07642 25600.00 16400.00 0.00 0.07756 25800.00 16400.00 0.00 0.07808 26000.00 16400.00 0.00 0.07812 25400.00 16600.00 0.00 0.07825 25600.00 16600.00 0.00 0.07940 25800.00 16600.00 0.00 0.07993 26000.00 16600.00 0.00 0.07996 25400.00 16800.00 0.00 0.08014 25600.00 16800.00 0.00 0.08131 25800.00 16800.00 0.00 0.08183 26000.00 16800.00 0.00 0.08185 25400.00 17000.00 0.00 0.08207 25600.00 17000.00 0.00 0.08325 25800.00 17000.00 0.00 0.08377 25400.00 17200.00 0.00 0.08404 25600.00 17200.00 0.00 0.08523 25800.00 17200.00 0.00 0.08574 25400.00 17400.00 0.00 0.08613 25600.00 17400.00 0.00 0.08732 25800.00 17400.00 0.00 0.08783 25400.00 17600.00 0.00 0.08840 25600.00 17600.00 0.00 0.08958 25800.00 17600.00 0.00 0.09006 25400.00 17800.00 0.00 0.09075 25600.00 17800.00 0.00 0.09191 25800.00 17800.00 0.00 0.09235 25400.00 18000.00 0.00 0.09311 25600.00 18000.00 0.00 0.09424 25800.00 18000.00 0.00 0.09462 25400.00 18200.00 0.00 0.09539 25600.00 18200.00 0.00 0.09647 25800.00 18200.00 0.00 0.09680 25400.00 18400.00 0.00 0.09751 25600.00 18400.00 0.00 0.09855 25800.00 18400.00 0.00 0.09883 25400.00 18600.00 0.00 0.09954 25600.00 18600.00 0.00 0.10056 25800.00 18600.00 0.00 0.10081 25400.00 18800.00 0.00 0.10167 25600.00 18800.00 0.00 0.10266 25800.00 18800.00 0.00 0.10288 25400.00 19000.00 0.00 0.10391 25600.00 19000.00 0.00 0.10488 25800.00 19000.00 0.00 0.10505 25400.00 19200.00 0.00 0.10620 25600.00 19200.00 0.00 0.10714 25800.00 19200.00 0.00 0.10729 25400.00 19400.00 0.00 0.10850 25600.00 19400.00 0.00 0.10943 25800.00 19400.00 0.00 0.10955 25400.00 19600.00 0.00 0.11073 25600.00 19600.00 0.00 0.11164 25800.00 19600.00 0.00 0.11174 25400.00 19800.00 0.00 0.11290 25600.00 19800.00 0.00 0.11380 25800.00 19800.00 0.00 0.11388 25400.00 20000.00 0.00 0.11519 25600.00 20000.00 0.00 0.11608 25800.00 20000.00 0.00 0.11612 25400.00 20200.00 0.00 0.11769 25600.00 20200.00 0.00 0.11856 25400.00 20400.00 0.00 0.12037 25600.00 20400.00 0.00 0.12121 25200.00 20600.00 0.00 0.12133 25400.00 20600.00 0.00 0.12320 25600.00 20600.00 0.00 0.12398 25200.00 20800.00 0.00 0.12422 25400.00 20800.00 0.00 0.12605 25600.00 20800.00 0.00 0.12676 25200.00 21000.00 0.00 0.12699 25400.00 21000.00 0.00 0.12875 25600.00 21000.00 0.00 0.12937 25200.00 21200.00 0.00 0.12967 25400.00 21200.00 0.00 0.13136 25600.00 21200.00 0.00 0.13190 25200.00 21400.00 0.00 0.13240 25400.00 21400.00 0.00 0.13402 25600.00 21400.00 0.00 0.13447 25200.00 21600.00 0.00 0.13515 25400.00 21600.00 0.00 0.13670 25600.00 21600.00 0.00 0.13707 25200.00 21800.00 0.00 0.13794 25400.00 21800.00 0.00 0.13944 25600.00 21800.00 0.00 0.13974 25200.00 22000.00 0.00 0.14085 25400.00 22000.00 0.00 0.14232 25600.00 22000.00 0.00 0.14257 25200.00 22200.00 0.00 0.14386 25400.00 22200.00 0.00 0.14531 25600.00 22200.00 0.00 0.14552 25200.00 22400.00 0.00 0.14707 25400.00 22400.00 0.00 0.14850 25600.00 22400.00 0.00 0.14865 25200.00 22600.00 0.00 0.15071 25400.00 22600.00 0.00 0.15209 25600.00 22600.00 0.00 0.15215 25200.00 22800.00 0.00 0.15474 25400.00 22800.00 0.00 0.15604 25000.00 23000.00 0.00 0.15639 25200.00 23000.00 0.00 0.15897 25400.00 23000.00 0.00 0.16015 25000.00 23200.00 0.00 0.16078 25200.00 23200.00 0.00 0.16327 25400.00 23200.00 0.00 0.16430 25000.00 23400.00 0.00 0.16504 25200.00 23400.00 0.00 0.16742 25400.00 23400.00 0.00 0.16829 25000.00 23600.00 0.00 0.16913 25200.00 23600.00 0.00 0.17139 25400.00 23600.00 0.00 0.17207 25000.00 23800.00 0.00 0.17344 25200.00 23800.00 0.00 0.17554 25400.00 23800.00 0.00 0.17598 25000.00 24000.00 0.00 0.17825 25200.00 24000.00 0.00 0.18010 25400.00 24000.00 0.00 0.18022 24800.00 24200.00 0.00 0.18024 25000.00 24200.00 0.00 0.18345 25200.00 24200.00 0.00 0.18495 24800.00 24400.00 0.00 0.18597 25000.00 24400.00 0.00 0.18878 25200.00 24400.00 0.00 0.18981 24800.00 24600.00 0.00 0.19133 25000.00 24600.00 0.00 0.19357 25200.00 24600.00 0.00 0.19400 24800.00 24800.00 0.00 0.19514 25000.00 24800.00 0.00 0.19670 24800.00 25000.00 0.00 0.19672 25000.00 25000.00 0.00 0.19759 24800.00 25200.00 40.00 0.19763 24800.00 25200.00 45.00 0.19768 24800.00 25200.00 50.00 0.19768 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24895.61 25114.12 48.24 0.19799 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/323.ctf NX, NY, NZ: 360 360 1 MODE : real