CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/330.mrc Output diagnostic file name Micrographs/330.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/330.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2432.000 8508.000 Mean, RMS : 4379.127 227.9359 TITLE 1: emi2mrc: 330.emi Mon Jan 20 00:54:40 2014 TITLE 2: kV 300, Mag 78000x, Def 27730, Spot 4, Int 55.12%, Obj 88.08%, Dif 73.67% TITLE 3: X -502.836, Y 535.417, Z -23.326, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03150 13000.00 8000.00 0.00 -0.03133 13200.00 8000.00 0.00 -0.03103 13400.00 8000.00 0.00 -0.03067 13600.00 8000.00 0.00 -0.03026 13800.00 8000.00 0.00 -0.02987 14000.00 8000.00 0.00 -0.02953 14200.00 8000.00 0.00 -0.02927 14400.00 8000.00 0.00 -0.02904 14600.00 8000.00 0.00 -0.02876 14800.00 8000.00 0.00 -0.02837 15000.00 8000.00 0.00 -0.02786 15200.00 8000.00 0.00 -0.02728 15400.00 8000.00 0.00 -0.02667 15600.00 8000.00 0.00 -0.02605 15800.00 8000.00 0.00 -0.02541 16000.00 8000.00 0.00 -0.02468 16200.00 8000.00 0.00 -0.02384 16400.00 8000.00 0.00 -0.02291 16600.00 8000.00 0.00 -0.02191 16800.00 8000.00 0.00 -0.02088 17000.00 8000.00 0.00 -0.01984 17200.00 8000.00 0.00 -0.01878 17400.00 8000.00 0.00 -0.01768 17600.00 8000.00 0.00 -0.01652 17800.00 8000.00 0.00 -0.01531 18000.00 8000.00 0.00 -0.01407 18200.00 8000.00 0.00 -0.01280 18400.00 8000.00 0.00 -0.01149 18600.00 8000.00 0.00 -0.01011 18800.00 8000.00 0.00 -0.00861 19000.00 8000.00 0.00 -0.00700 19200.00 8000.00 0.00 -0.00529 19400.00 8000.00 0.00 -0.00357 19600.00 8000.00 0.00 -0.00191 19800.00 8000.00 0.00 -0.00035 20000.00 8000.00 0.00 0.00112 20200.00 8000.00 0.00 0.00251 20400.00 8000.00 0.00 0.00388 20600.00 8000.00 0.00 0.00526 20800.00 8000.00 0.00 0.00667 21000.00 8000.00 0.00 0.00812 21200.00 8000.00 0.00 0.00964 21400.00 8000.00 0.00 0.01127 21600.00 8000.00 0.00 0.01305 21800.00 8000.00 0.00 0.01498 22000.00 8000.00 0.00 0.01706 22200.00 8000.00 0.00 0.01924 22400.00 8000.00 0.00 0.02143 22600.00 8000.00 0.00 0.02357 22800.00 8000.00 0.00 0.02563 23000.00 8000.00 0.00 0.02763 23200.00 8000.00 0.00 0.02964 23400.00 8000.00 0.00 0.03176 23600.00 8000.00 0.00 0.03406 23800.00 8000.00 0.00 0.03654 24000.00 8000.00 0.00 0.03910 24200.00 8000.00 0.00 0.04153 24400.00 8000.00 0.00 0.04360 24600.00 8000.00 0.00 0.04515 24800.00 8000.00 0.00 0.04613 25000.00 8000.00 0.00 0.04660 25200.00 8000.00 0.00 0.04675 25000.00 8200.00 0.00 0.04707 25200.00 8200.00 0.00 0.04719 25000.00 8400.00 0.00 0.04751 25200.00 8400.00 0.00 0.04761 24800.00 8600.00 0.00 0.04762 25000.00 8600.00 0.00 0.04804 25200.00 8600.00 0.00 0.04812 24800.00 8800.00 0.00 0.04828 25000.00 8800.00 0.00 0.04868 25200.00 8800.00 0.00 0.04874 24800.00 9000.00 0.00 0.04902 25000.00 9000.00 0.00 0.04940 25200.00 9000.00 0.00 0.04942 24800.00 9200.00 0.00 0.04979 25000.00 9200.00 0.00 0.05014 24800.00 9400.00 0.00 0.05053 25000.00 9400.00 0.00 0.05083 24800.00 9600.00 0.00 0.05118 25000.00 9600.00 0.00 0.05143 24800.00 9800.00 0.00 0.05177 25000.00 9800.00 0.00 0.05198 24800.00 10000.00 0.00 0.05234 25000.00 10000.00 0.00 0.05251 24800.00 10200.00 0.00 0.05290 25000.00 10200.00 0.00 0.05306 24800.00 10400.00 0.00 0.05350 25000.00 10400.00 0.00 0.05366 24800.00 10600.00 0.00 0.05418 25000.00 10600.00 0.00 0.05437 24800.00 10800.00 0.00 0.05496 25000.00 10800.00 0.00 0.05518 24800.00 11000.00 0.00 0.05586 25000.00 11000.00 0.00 0.05611 24800.00 11200.00 0.00 0.05689 25000.00 11200.00 0.00 0.05716 24600.00 11400.00 0.00 0.05716 24800.00 11400.00 0.00 0.05797 25000.00 11400.00 0.00 0.05824 24800.00 11600.00 0.00 0.05905 25000.00 11600.00 0.00 0.05931 24800.00 11800.00 0.00 0.06010 25000.00 11800.00 0.00 0.06035 24600.00 12000.00 0.00 0.06035 24800.00 12000.00 0.00 0.06113 25000.00 12000.00 0.00 0.06136 24600.00 12200.00 0.00 0.06141 24800.00 12200.00 0.00 0.06217 25000.00 12200.00 0.00 0.06238 24600.00 12400.00 0.00 0.06255 24800.00 12400.00 0.00 0.06330 25000.00 12400.00 0.00 0.06350 24600.00 12600.00 0.00 0.06376 24800.00 12600.00 0.00 0.06451 25000.00 12600.00 0.00 0.06470 24600.00 12800.00 0.00 0.06500 24800.00 12800.00 0.00 0.06574 25000.00 12800.00 0.00 0.06593 24600.00 13000.00 0.00 0.06626 24800.00 13000.00 0.00 0.06699 25000.00 13000.00 0.00 0.06717 24600.00 13200.00 0.00 0.06752 24800.00 13200.00 0.00 0.06824 25000.00 13200.00 0.00 0.06840 24600.00 13400.00 0.00 0.06877 24800.00 13400.00 0.00 0.06948 25000.00 13400.00 0.00 0.06962 24600.00 13600.00 0.00 0.07008 24800.00 13600.00 0.00 0.07077 25000.00 13600.00 0.00 0.07089 24600.00 13800.00 0.00 0.07147 24800.00 13800.00 0.00 0.07213 25000.00 13800.00 0.00 0.07223 24600.00 14000.00 0.00 0.07285 24800.00 14000.00 0.00 0.07349 25000.00 14000.00 0.00 0.07356 24600.00 14200.00 0.00 0.07419 24800.00 14200.00 0.00 0.07480 25000.00 14200.00 0.00 0.07484 24600.00 14400.00 0.00 0.07548 24800.00 14400.00 0.00 0.07606 25000.00 14400.00 0.00 0.07607 24600.00 14600.00 0.00 0.07673 24800.00 14600.00 0.00 0.07729 24600.00 14800.00 0.00 0.07803 24800.00 14800.00 0.00 0.07858 24600.00 15000.00 0.00 0.07946 24800.00 15000.00 0.00 0.08001 24600.00 15200.00 0.00 0.08099 24800.00 15200.00 0.00 0.08155 24600.00 15400.00 0.00 0.08256 24800.00 15400.00 0.00 0.08313 24600.00 15600.00 0.00 0.08413 24800.00 15600.00 0.00 0.08472 25000.00 15600.00 0.00 0.08472 24600.00 15800.00 0.00 0.08569 24800.00 15800.00 0.00 0.08629 25000.00 15800.00 0.00 0.08630 24600.00 16000.00 0.00 0.08730 24800.00 16000.00 0.00 0.08791 25000.00 16000.00 0.00 0.08792 24600.00 16200.00 0.00 0.08908 24800.00 16200.00 0.00 0.08969 24600.00 16400.00 0.00 0.09104 24800.00 16400.00 0.00 0.09165 24400.00 16600.00 0.00 0.09167 24600.00 16600.00 0.00 0.09312 24800.00 16600.00 0.00 0.09371 24400.00 16800.00 0.00 0.09381 24600.00 16800.00 0.00 0.09525 24800.00 16800.00 0.00 0.09582 24400.00 17000.00 0.00 0.09593 24600.00 17000.00 0.00 0.09736 24800.00 17000.00 0.00 0.09790 24400.00 17200.00 0.00 0.09801 24600.00 17200.00 0.00 0.09942 24800.00 17200.00 0.00 0.09991 24400.00 17400.00 0.00 0.10013 24600.00 17400.00 0.00 0.10151 24800.00 17400.00 0.00 0.10196 24400.00 17600.00 0.00 0.10238 24600.00 17600.00 0.00 0.10372 24800.00 17600.00 0.00 0.10411 24400.00 17800.00 0.00 0.10468 24600.00 17800.00 0.00 0.10598 24800.00 17800.00 0.00 0.10632 24400.00 18000.00 0.00 0.10702 24600.00 18000.00 0.00 0.10828 24800.00 18000.00 0.00 0.10857 24400.00 18200.00 0.00 0.10937 24600.00 18200.00 0.00 0.11060 24800.00 18200.00 0.00 0.11085 24400.00 18400.00 0.00 0.11169 24600.00 18400.00 0.00 0.11289 24800.00 18400.00 0.00 0.11310 24400.00 18600.00 0.00 0.11402 24600.00 18600.00 0.00 0.11520 24800.00 18600.00 0.00 0.11536 24400.00 18800.00 0.00 0.11650 24600.00 18800.00 0.00 0.11764 24800.00 18800.00 0.00 0.11777 24400.00 19000.00 0.00 0.11906 24600.00 19000.00 0.00 0.12019 24800.00 19000.00 0.00 0.12029 24400.00 19200.00 0.00 0.12165 24600.00 19200.00 0.00 0.12277 24800.00 19200.00 0.00 0.12284 24400.00 19400.00 0.00 0.12428 24600.00 19400.00 0.00 0.12538 24800.00 19400.00 0.00 0.12542 24400.00 19600.00 0.00 0.12685 24600.00 19600.00 0.00 0.12793 24400.00 19800.00 0.00 0.12937 24600.00 19800.00 0.00 0.13042 24400.00 20000.00 0.00 0.13200 24600.00 20000.00 0.00 0.13301 24400.00 20200.00 0.00 0.13480 24600.00 20200.00 0.00 0.13576 24400.00 20400.00 0.00 0.13767 24600.00 20400.00 0.00 0.13857 24400.00 20600.00 0.00 0.14060 24600.00 20600.00 0.00 0.14144 24200.00 20800.00 0.00 0.14154 24400.00 20800.00 0.00 0.14354 24600.00 20800.00 0.00 0.14432 24200.00 21000.00 0.00 0.14443 24400.00 21000.00 0.00 0.14638 24600.00 21000.00 0.00 0.14710 24200.00 21200.00 0.00 0.14735 24400.00 21200.00 0.00 0.14925 24600.00 21200.00 0.00 0.14989 24200.00 21400.00 0.00 0.15046 24400.00 21400.00 0.00 0.15229 24600.00 21400.00 0.00 0.15285 24200.00 21600.00 0.00 0.15371 24400.00 21600.00 0.00 0.15549 24600.00 21600.00 0.00 0.15596 24200.00 21800.00 0.00 0.15713 24400.00 21800.00 0.00 0.15884 24600.00 21800.00 0.00 0.15922 24200.00 22000.00 0.00 0.16076 24400.00 22000.00 0.00 0.16240 24600.00 22000.00 0.00 0.16269 24200.00 22200.00 0.00 0.16449 24400.00 22200.00 0.00 0.16608 24600.00 22200.00 0.00 0.16627 24200.00 22400.00 0.00 0.16835 24400.00 22400.00 0.00 0.16987 24600.00 22400.00 0.00 0.16996 24200.00 22600.00 0.00 0.17250 24400.00 22600.00 0.00 0.17394 24000.00 22800.00 0.00 0.17420 24200.00 22800.00 0.00 0.17690 24400.00 22800.00 0.00 0.17824 24000.00 23000.00 0.00 0.17886 24200.00 23000.00 0.00 0.18149 24400.00 23000.00 0.00 0.18266 24000.00 23200.00 0.00 0.18383 24200.00 23200.00 0.00 0.18631 24400.00 23200.00 0.00 0.18724 24000.00 23400.00 0.00 0.18885 24200.00 23400.00 0.00 0.19109 24400.00 23400.00 0.00 0.19167 24000.00 23600.00 0.00 0.19345 24200.00 23600.00 0.00 0.19536 24400.00 23600.00 0.00 0.19551 24000.00 23800.00 0.00 0.19738 24200.00 23800.00 0.00 0.19885 24000.00 24000.00 0.00 0.20009 24200.00 24000.00 0.00 0.20098 24000.00 24200.00 10.00 0.20103 24000.00 24200.00 15.00 0.20118 24000.00 24200.00 20.00 0.20133 24000.00 24200.00 25.00 0.20146 24000.00 24200.00 30.00 0.20157 24000.00 24200.00 35.00 0.20166 24000.00 24200.00 40.00 0.20172 24000.00 24200.00 45.00 0.20175 24000.00 24200.00 50.00 0.20176 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24009.13 24275.91 50.00 0.20191 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/330.ctf NX, NY, NZ: 360 360 1 MODE : real