CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/345.mrc Output diagnostic file name Micrographs/345.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/345.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2256.000 7896.000 Mean, RMS : 4379.300 221.2363 TITLE 1: emi2mrc: 345.emi Mon Jan 20 01:06:47 2014 TITLE 2: kV 300, Mag 78000x, Def 27730, Spot 4, Int 55.12%, Obj 88.08%, Dif 73.67% TITLE 3: X -487.714, Y 557.731, Z -23.326, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01745 8200.00 8000.00 0.00 -0.01726 8400.00 8000.00 0.00 -0.01703 8600.00 8000.00 0.00 -0.01677 8800.00 8000.00 0.00 -0.01653 9000.00 8000.00 0.00 -0.01627 9200.00 8000.00 0.00 -0.01595 9400.00 8000.00 0.00 -0.01556 9600.00 8000.00 0.00 -0.01506 9800.00 8000.00 0.00 -0.01447 10000.00 8000.00 0.00 -0.01385 10200.00 8000.00 0.00 -0.01325 10400.00 8000.00 0.00 -0.01267 10600.00 8000.00 0.00 -0.01212 10800.00 8000.00 0.00 -0.01158 11000.00 8000.00 0.00 -0.01100 11200.00 8000.00 0.00 -0.01038 11400.00 8000.00 0.00 -0.00967 11600.00 8000.00 0.00 -0.00886 11800.00 8000.00 0.00 -0.00794 12000.00 8000.00 0.00 -0.00696 12200.00 8000.00 0.00 -0.00598 12400.00 8000.00 0.00 -0.00506 12600.00 8000.00 0.00 -0.00420 12800.00 8000.00 0.00 -0.00335 13000.00 8000.00 0.00 -0.00240 13200.00 8000.00 0.00 -0.00126 13400.00 8000.00 0.00 0.00011 13600.00 8000.00 0.00 0.00167 13800.00 8000.00 0.00 0.00333 14000.00 8000.00 0.00 0.00502 14200.00 8000.00 0.00 0.00672 14400.00 8000.00 0.00 0.00850 14600.00 8000.00 0.00 0.01044 14800.00 8000.00 0.00 0.01258 15000.00 8000.00 0.00 0.01491 15200.00 8000.00 0.00 0.01734 15400.00 8000.00 0.00 0.01978 15600.00 8000.00 0.00 0.02219 15800.00 8000.00 0.00 0.02456 16000.00 8000.00 0.00 0.02692 16200.00 8000.00 0.00 0.02931 16400.00 8000.00 0.00 0.03176 16600.00 8000.00 0.00 0.03426 16800.00 8000.00 0.00 0.03685 17000.00 8000.00 0.00 0.03958 17200.00 8000.00 0.00 0.04250 17400.00 8000.00 0.00 0.04561 17600.00 8000.00 0.00 0.04893 17800.00 8000.00 0.00 0.05235 18000.00 8000.00 0.00 0.05583 18200.00 8000.00 0.00 0.05936 18400.00 8000.00 0.00 0.06303 18600.00 8000.00 0.00 0.06691 18800.00 8000.00 0.00 0.07100 19000.00 8000.00 0.00 0.07512 19200.00 8000.00 0.00 0.07891 19400.00 8000.00 0.00 0.08202 19600.00 8000.00 0.00 0.08419 19800.00 8000.00 0.00 0.08541 20000.00 8000.00 0.00 0.08590 20200.00 8000.00 0.00 0.08597 19800.00 8200.00 0.00 0.08634 20000.00 8200.00 0.00 0.08684 20200.00 8200.00 0.00 0.08691 19800.00 8400.00 0.00 0.08721 20000.00 8400.00 0.00 0.08772 20200.00 8400.00 0.00 0.08780 19800.00 8600.00 0.00 0.08824 20000.00 8600.00 0.00 0.08876 20200.00 8600.00 0.00 0.08884 19800.00 8800.00 0.00 0.08949 20000.00 8800.00 0.00 0.09001 20200.00 8800.00 0.00 0.09009 19800.00 9000.00 0.00 0.09089 20000.00 9000.00 0.00 0.09141 20200.00 9000.00 0.00 0.09149 19800.00 9200.00 0.00 0.09241 20000.00 9200.00 0.00 0.09293 20200.00 9200.00 0.00 0.09299 19800.00 9400.00 0.00 0.09396 20000.00 9400.00 0.00 0.09446 20200.00 9400.00 0.00 0.09450 19800.00 9600.00 0.00 0.09546 20000.00 9600.00 0.00 0.09595 20200.00 9600.00 0.00 0.09596 19800.00 9800.00 0.00 0.09699 20000.00 9800.00 0.00 0.09746 19800.00 10000.00 0.00 0.09859 20000.00 10000.00 0.00 0.09902 19800.00 10200.00 0.00 0.10021 20000.00 10200.00 0.00 0.10060 19600.00 10400.00 0.00 0.10064 19800.00 10400.00 0.00 0.10185 20000.00 10400.00 0.00 0.10219 19600.00 10600.00 0.00 0.10237 19800.00 10600.00 0.00 0.10354 20000.00 10600.00 0.00 0.10384 19600.00 10800.00 0.00 0.10416 19800.00 10800.00 0.00 0.10529 20000.00 10800.00 0.00 0.10555 19600.00 11000.00 0.00 0.10607 19800.00 11000.00 0.00 0.10717 20000.00 11000.00 0.00 0.10739 19600.00 11200.00 0.00 0.10815 19800.00 11200.00 0.00 0.10921 20000.00 11200.00 0.00 0.10941 19600.00 11400.00 0.00 0.11025 19800.00 11400.00 0.00 0.11129 20000.00 11400.00 0.00 0.11147 19600.00 11600.00 0.00 0.11228 19800.00 11600.00 0.00 0.11332 20000.00 11600.00 0.00 0.11350 19600.00 11800.00 0.00 0.11425 19800.00 11800.00 0.00 0.11531 20000.00 11800.00 0.00 0.11551 19600.00 12000.00 0.00 0.11623 19800.00 12000.00 0.00 0.11732 20000.00 12000.00 0.00 0.11754 19600.00 12200.00 0.00 0.11837 19800.00 12200.00 0.00 0.11948 20000.00 12200.00 0.00 0.11971 19600.00 12400.00 0.00 0.12086 19800.00 12400.00 0.00 0.12197 20000.00 12400.00 0.00 0.12218 19600.00 12600.00 0.00 0.12362 19800.00 12600.00 0.00 0.12470 20000.00 12600.00 0.00 0.12488 19600.00 12800.00 0.00 0.12641 19800.00 12800.00 0.00 0.12746 20000.00 12800.00 0.00 0.12757 19600.00 13000.00 0.00 0.12910 19800.00 13000.00 0.00 0.13010 20000.00 13000.00 0.00 0.13014 19600.00 13200.00 0.00 0.13160 19800.00 13200.00 0.00 0.13254 19600.00 13400.00 0.00 0.13394 19800.00 13400.00 0.00 0.13481 19600.00 13600.00 0.00 0.13639 19800.00 13600.00 0.00 0.13721 19600.00 13800.00 0.00 0.13914 19800.00 13800.00 0.00 0.13990 19400.00 14000.00 0.00 0.14000 19600.00 14000.00 0.00 0.14210 19800.00 14000.00 0.00 0.14283 19400.00 14200.00 0.00 0.14312 19600.00 14200.00 0.00 0.14520 19800.00 14200.00 0.00 0.14588 19400.00 14400.00 0.00 0.14628 19600.00 14400.00 0.00 0.14833 19800.00 14400.00 0.00 0.14895 19400.00 14600.00 0.00 0.14934 19600.00 14600.00 0.00 0.15133 19800.00 14600.00 0.00 0.15188 19400.00 14800.00 0.00 0.15236 19600.00 14800.00 0.00 0.15430 19800.00 14800.00 0.00 0.15478 19400.00 15000.00 0.00 0.15555 19600.00 15000.00 0.00 0.15743 19800.00 15000.00 0.00 0.15784 19400.00 15200.00 0.00 0.15883 19600.00 15200.00 0.00 0.16066 19800.00 15200.00 0.00 0.16100 19400.00 15400.00 0.00 0.16211 19600.00 15400.00 0.00 0.16390 19800.00 15400.00 0.00 0.16417 19400.00 15600.00 0.00 0.16542 19600.00 15600.00 0.00 0.16718 19800.00 15600.00 0.00 0.16739 19400.00 15800.00 0.00 0.16873 19600.00 15800.00 0.00 0.17045 19800.00 15800.00 0.00 0.17059 19400.00 16000.00 0.00 0.17208 19600.00 16000.00 0.00 0.17375 19800.00 16000.00 0.00 0.17379 19400.00 16200.00 0.00 0.17566 19600.00 16200.00 0.00 0.17727 19400.00 16400.00 0.00 0.17945 19600.00 16400.00 0.00 0.18098 19400.00 16600.00 0.00 0.18317 19600.00 16600.00 0.00 0.18460 19400.00 16800.00 0.00 0.18670 19600.00 16800.00 0.00 0.18802 19400.00 17000.00 0.00 0.19001 19600.00 17000.00 0.00 0.19120 19400.00 17200.00 0.00 0.19313 19600.00 17200.00 0.00 0.19416 19400.00 17400.00 0.00 0.19637 19600.00 17400.00 0.00 0.19722 19200.00 17600.00 0.00 0.19724 19400.00 17600.00 0.00 0.19988 19600.00 17600.00 0.00 0.20053 19200.00 17800.00 0.00 0.20091 19400.00 17800.00 0.00 0.20339 19600.00 17800.00 0.00 0.20381 19200.00 18000.00 0.00 0.20432 19400.00 18000.00 0.00 0.20662 19600.00 18000.00 0.00 0.20680 19200.00 18200.00 0.00 0.20736 19400.00 18200.00 0.00 0.20947 19200.00 18400.00 0.00 0.20992 19400.00 18400.00 0.00 0.21186 19200.00 18600.00 0.00 0.21235 19400.00 18600.00 0.00 0.21410 19200.00 18800.00 0.00 0.21516 19400.00 18800.00 0.00 0.21664 19200.00 19000.00 0.00 0.21809 19400.00 19000.00 0.00 0.21917 19200.00 19200.00 0.00 0.22036 19400.00 19200.00 0.00 0.22094 19200.00 19400.00 0.00 0.22149 19400.00 19400.00 0.00 0.22151 19200.00 19400.00 5.00 0.22156 19200.00 19400.00 10.00 0.22163 19200.00 19400.00 15.00 0.22168 19200.00 19400.00 20.00 0.22172 19200.00 19400.00 25.00 0.22174 19200.00 19400.00 30.00 0.22175 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19270.60 19430.37 19.30 0.22190 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/345.ctf NX, NY, NZ: 360 360 1 MODE : real