CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/346.mrc Output diagnostic file name Micrographs/346.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/346.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2202.000 9928.000 Mean, RMS : 4374.871 223.2404 TITLE 1: emi2mrc: 346.emi Mon Jan 20 01:07:31 2014 TITLE 2: kV 300, Mag 78000x, Def 27730, Spot 4, Int 55.12%, Obj 88.08%, Dif 73.67% TITLE 3: X -485.971, Y 559.975, Z -23.326, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 118 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01752 8200.00 8000.00 0.00 -0.01732 8400.00 8000.00 0.00 -0.01717 8600.00 8000.00 0.00 -0.01715 9400.00 8000.00 0.00 -0.01695 9600.00 8000.00 0.00 -0.01652 9800.00 8000.00 0.00 -0.01601 10000.00 8000.00 0.00 -0.01549 10200.00 8000.00 0.00 -0.01496 10400.00 8000.00 0.00 -0.01437 10600.00 8000.00 0.00 -0.01372 10800.00 8000.00 0.00 -0.01304 11000.00 8000.00 0.00 -0.01238 11200.00 8000.00 0.00 -0.01178 11400.00 8000.00 0.00 -0.01123 11600.00 8000.00 0.00 -0.01068 11800.00 8000.00 0.00 -0.01005 12000.00 8000.00 0.00 -0.00934 12200.00 8000.00 0.00 -0.00854 12400.00 8000.00 0.00 -0.00768 12600.00 8000.00 0.00 -0.00673 12800.00 8000.00 0.00 -0.00568 13000.00 8000.00 0.00 -0.00450 13200.00 8000.00 0.00 -0.00324 13400.00 8000.00 0.00 -0.00196 13600.00 8000.00 0.00 -0.00070 13800.00 8000.00 0.00 0.00057 14000.00 8000.00 0.00 0.00192 14200.00 8000.00 0.00 0.00343 14400.00 8000.00 0.00 0.00511 14600.00 8000.00 0.00 0.00690 14800.00 8000.00 0.00 0.00871 15000.00 8000.00 0.00 0.01051 15200.00 8000.00 0.00 0.01236 15400.00 8000.00 0.00 0.01437 15600.00 8000.00 0.00 0.01664 15800.00 8000.00 0.00 0.01915 16000.00 8000.00 0.00 0.02183 16200.00 8000.00 0.00 0.02458 16400.00 8000.00 0.00 0.02735 16600.00 8000.00 0.00 0.03011 16800.00 8000.00 0.00 0.03285 17000.00 8000.00 0.00 0.03557 17200.00 8000.00 0.00 0.03817 17400.00 8000.00 0.00 0.04064 17600.00 8000.00 0.00 0.04304 17800.00 8000.00 0.00 0.04551 18000.00 8000.00 0.00 0.04820 18200.00 8000.00 0.00 0.05120 18400.00 8000.00 0.00 0.05444 18600.00 8000.00 0.00 0.05778 18800.00 8000.00 0.00 0.06111 19000.00 8000.00 0.00 0.06446 19200.00 8000.00 0.00 0.06794 19400.00 8000.00 0.00 0.07170 19600.00 8000.00 0.00 0.07571 19800.00 8000.00 0.00 0.07974 20000.00 8000.00 0.00 0.08342 20200.00 8000.00 0.00 0.08635 20400.00 8000.00 0.00 0.08823 20600.00 8000.00 0.00 0.08909 20800.00 8000.00 0.00 0.08914 20600.00 8200.00 0.00 0.08977 20800.00 8200.00 0.00 0.08980 20600.00 8400.00 0.00 0.09050 20800.00 8400.00 0.00 0.09050 20400.00 8600.00 0.00 0.09064 20600.00 8600.00 0.00 0.09143 20400.00 8800.00 0.00 0.09177 20600.00 8800.00 0.00 0.09254 20400.00 9000.00 0.00 0.09291 20600.00 9000.00 0.00 0.09367 20400.00 9200.00 0.00 0.09403 20600.00 9200.00 0.00 0.09478 20400.00 9400.00 0.00 0.09509 20600.00 9400.00 0.00 0.09583 20400.00 9600.00 0.00 0.09614 20600.00 9600.00 0.00 0.09687 20400.00 9800.00 0.00 0.09737 20600.00 9800.00 0.00 0.09810 20400.00 10000.00 0.00 0.09883 20600.00 10000.00 0.00 0.09956 20400.00 10200.00 0.00 0.10038 20600.00 10200.00 0.00 0.10111 20400.00 10400.00 0.00 0.10185 20600.00 10400.00 0.00 0.10259 20400.00 10600.00 0.00 0.10317 20600.00 10600.00 0.00 0.10390 20400.00 10800.00 0.00 0.10430 20600.00 10800.00 0.00 0.10503 20400.00 11000.00 0.00 0.10543 20600.00 11000.00 0.00 0.10615 20400.00 11200.00 0.00 0.10679 20600.00 11200.00 0.00 0.10750 20400.00 11400.00 0.00 0.10840 20600.00 11400.00 0.00 0.10910 20400.00 11600.00 0.00 0.11016 20600.00 11600.00 0.00 0.11085 20400.00 11800.00 0.00 0.11196 20600.00 11800.00 0.00 0.11262 20400.00 12000.00 0.00 0.11365 20600.00 12000.00 0.00 0.11430 20400.00 12200.00 0.00 0.11528 20600.00 12200.00 0.00 0.11591 20400.00 12400.00 0.00 0.11702 20600.00 12400.00 0.00 0.11764 20400.00 12600.00 0.00 0.11895 20600.00 12600.00 0.00 0.11956 20400.00 12800.00 0.00 0.12104 20600.00 12800.00 0.00 0.12165 20400.00 13000.00 0.00 0.12330 20600.00 13000.00 0.00 0.12390 20400.00 13200.00 0.00 0.12565 20600.00 13200.00 0.00 0.12625 20200.00 13400.00 0.00 0.12626 20400.00 13400.00 0.00 0.12805 20600.00 13400.00 0.00 0.12864 20200.00 13600.00 0.00 0.12884 20400.00 13600.00 0.00 0.13064 20600.00 13600.00 0.00 0.13123 20200.00 13800.00 0.00 0.13167 20400.00 13800.00 0.00 0.13346 20600.00 13800.00 0.00 0.13404 20200.00 14000.00 0.00 0.13458 20400.00 14000.00 0.00 0.13636 20600.00 14000.00 0.00 0.13691 20200.00 14200.00 0.00 0.13747 20400.00 14200.00 0.00 0.13923 20600.00 14200.00 0.00 0.13973 20200.00 14400.00 0.00 0.14024 20400.00 14400.00 0.00 0.14198 20600.00 14400.00 0.00 0.14245 20200.00 14600.00 0.00 0.14287 20400.00 14600.00 0.00 0.14459 20600.00 14600.00 0.00 0.14503 20200.00 14800.00 0.00 0.14554 20400.00 14800.00 0.00 0.14725 20600.00 14800.00 0.00 0.14766 20200.00 15000.00 0.00 0.14852 20400.00 15000.00 0.00 0.15022 20600.00 15000.00 0.00 0.15057 20200.00 15200.00 0.00 0.15179 20400.00 15200.00 0.00 0.15345 20600.00 15200.00 0.00 0.15374 20200.00 15400.00 0.00 0.15520 20400.00 15400.00 0.00 0.15681 20600.00 15400.00 0.00 0.15703 20200.00 15600.00 0.00 0.15865 20400.00 15600.00 0.00 0.16020 20600.00 15600.00 0.00 0.16032 20200.00 15800.00 0.00 0.16194 20400.00 15800.00 0.00 0.16341 20600.00 15800.00 0.00 0.16342 20200.00 16000.00 0.00 0.16501 20400.00 16000.00 0.00 0.16639 20200.00 16200.00 0.00 0.16811 20400.00 16200.00 0.00 0.16939 20200.00 16400.00 0.00 0.17131 20400.00 16400.00 0.00 0.17249 20200.00 16600.00 0.00 0.17451 20400.00 16600.00 0.00 0.17559 20200.00 16800.00 0.00 0.17770 20400.00 16800.00 0.00 0.17868 20200.00 17000.00 0.00 0.18083 20400.00 17000.00 0.00 0.18173 20200.00 17200.00 0.00 0.18387 20400.00 17200.00 0.00 0.18466 20200.00 17400.00 0.00 0.18702 20400.00 17400.00 0.00 0.18769 20000.00 17600.00 0.00 0.18806 20200.00 17600.00 0.00 0.19047 20400.00 17600.00 0.00 0.19097 20000.00 17800.00 0.00 0.19177 20200.00 17800.00 0.00 0.19403 20400.00 17800.00 0.00 0.19436 20000.00 18000.00 0.00 0.19548 20200.00 18000.00 0.00 0.19758 20400.00 18000.00 0.00 0.19770 20000.00 18200.00 0.00 0.19908 20200.00 18200.00 0.00 0.20102 20000.00 18400.00 0.00 0.20245 20200.00 18400.00 0.00 0.20424 20000.00 18600.00 0.00 0.20582 20200.00 18600.00 0.00 0.20744 20000.00 18800.00 0.00 0.20960 20200.00 18800.00 0.00 0.21099 20000.00 19000.00 0.00 0.21369 20200.00 19000.00 0.00 0.21479 19800.00 19200.00 0.00 0.21487 20000.00 19200.00 0.00 0.21775 20200.00 19200.00 0.00 0.21848 19800.00 19400.00 0.00 0.21902 20000.00 19400.00 0.00 0.22145 20200.00 19400.00 0.00 0.22174 19800.00 19600.00 0.00 0.22226 20000.00 19600.00 0.00 0.22417 20000.00 19800.00 0.00 0.22543 20000.00 20000.00 0.00 0.22557 19800.00 20200.00 25.00 0.22565 19800.00 20200.00 30.00 0.22588 19800.00 20200.00 35.00 0.22607 19800.00 20200.00 40.00 0.22621 19800.00 20200.00 45.00 0.22630 19800.00 20200.00 50.00 0.22632 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19780.32 20154.34 52.70 0.22638 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/346.ctf NX, NY, NZ: 360 360 1 MODE : real