CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/368.mrc Output diagnostic file name Micrographs/368.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/368.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2398.000 7522.000 Mean, RMS : 4375.563 221.0584 TITLE 1: emi2mrc: 368.emi Mon Jan 20 01:26:39 2014 TITLE 2: kV 300, Mag 78000x, Def 24450, Spot 4, Int 55.12%, Obj 88.08%, Dif 73.67% TITLE 3: X -495.238, Y 557.278, Z -22.878, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01259 8200.00 8000.00 0.00 -0.01222 8400.00 8000.00 0.00 -0.01178 8600.00 8000.00 0.00 -0.01137 8800.00 8000.00 0.00 -0.01105 9000.00 8000.00 0.00 -0.01073 9200.00 8000.00 0.00 -0.01034 9400.00 8000.00 0.00 -0.00986 9600.00 8000.00 0.00 -0.00931 9800.00 8000.00 0.00 -0.00874 10000.00 8000.00 0.00 -0.00822 10200.00 8000.00 0.00 -0.00775 10400.00 8000.00 0.00 -0.00727 10600.00 8000.00 0.00 -0.00672 10800.00 8000.00 0.00 -0.00604 11000.00 8000.00 0.00 -0.00522 11200.00 8000.00 0.00 -0.00428 11400.00 8000.00 0.00 -0.00324 11600.00 8000.00 0.00 -0.00209 11800.00 8000.00 0.00 -0.00085 12000.00 8000.00 0.00 0.00047 12200.00 8000.00 0.00 0.00182 12400.00 8000.00 0.00 0.00320 12600.00 8000.00 0.00 0.00461 12800.00 8000.00 0.00 0.00611 13000.00 8000.00 0.00 0.00773 13200.00 8000.00 0.00 0.00946 13400.00 8000.00 0.00 0.01126 13600.00 8000.00 0.00 0.01307 13800.00 8000.00 0.00 0.01487 14000.00 8000.00 0.00 0.01669 14200.00 8000.00 0.00 0.01860 14400.00 8000.00 0.00 0.02068 14600.00 8000.00 0.00 0.02295 14800.00 8000.00 0.00 0.02535 15000.00 8000.00 0.00 0.02785 15200.00 8000.00 0.00 0.03038 15400.00 8000.00 0.00 0.03291 15600.00 8000.00 0.00 0.03544 15800.00 8000.00 0.00 0.03799 16000.00 8000.00 0.00 0.04055 16200.00 8000.00 0.00 0.04317 16400.00 8000.00 0.00 0.04588 16600.00 8000.00 0.00 0.04873 16800.00 8000.00 0.00 0.05171 17000.00 8000.00 0.00 0.05481 17200.00 8000.00 0.00 0.05795 17400.00 8000.00 0.00 0.06105 17600.00 8000.00 0.00 0.06412 17800.00 8000.00 0.00 0.06721 18000.00 8000.00 0.00 0.07040 18200.00 8000.00 0.00 0.07378 18400.00 8000.00 0.00 0.07731 18600.00 8000.00 0.00 0.08083 18800.00 8000.00 0.00 0.08410 19000.00 8000.00 0.00 0.08687 19200.00 8000.00 0.00 0.08896 19400.00 8000.00 0.00 0.09036 19600.00 8000.00 0.00 0.09119 19800.00 8000.00 0.00 0.09165 20000.00 8000.00 0.00 0.09190 20200.00 8000.00 0.00 0.09204 19600.00 8200.00 0.00 0.09234 19800.00 8200.00 0.00 0.09278 20000.00 8200.00 0.00 0.09303 20200.00 8200.00 0.00 0.09315 19600.00 8400.00 0.00 0.09353 19800.00 8400.00 0.00 0.09396 20000.00 8400.00 0.00 0.09419 20200.00 8400.00 0.00 0.09431 19600.00 8600.00 0.00 0.09494 19800.00 8600.00 0.00 0.09536 20000.00 8600.00 0.00 0.09558 20200.00 8600.00 0.00 0.09567 19400.00 8800.00 0.00 0.09578 19600.00 8800.00 0.00 0.09656 19800.00 8800.00 0.00 0.09695 20000.00 8800.00 0.00 0.09715 20200.00 8800.00 0.00 0.09722 19400.00 9000.00 0.00 0.09744 19600.00 9000.00 0.00 0.09820 19800.00 9000.00 0.00 0.09856 20000.00 9000.00 0.00 0.09871 20200.00 9000.00 0.00 0.09874 19400.00 9200.00 0.00 0.09899 19600.00 9200.00 0.00 0.09970 19800.00 9200.00 0.00 0.10002 20000.00 9200.00 0.00 0.10013 19400.00 9400.00 0.00 0.10028 19600.00 9400.00 0.00 0.10095 19800.00 9400.00 0.00 0.10122 20000.00 9400.00 0.00 0.10130 19400.00 9600.00 0.00 0.10131 19600.00 9600.00 0.00 0.10194 19800.00 9600.00 0.00 0.10218 20000.00 9600.00 0.00 0.10223 19400.00 9800.00 0.00 0.10229 19600.00 9800.00 0.00 0.10289 19800.00 9800.00 0.00 0.10312 20000.00 9800.00 0.00 0.10315 19400.00 10000.00 0.00 0.10340 19600.00 10000.00 0.00 0.10399 19800.00 10000.00 0.00 0.10420 20000.00 10000.00 0.00 0.10422 19400.00 10200.00 0.00 0.10464 19600.00 10200.00 0.00 0.10522 19800.00 10200.00 0.00 0.10542 20000.00 10200.00 0.00 0.10543 19400.00 10400.00 0.00 0.10601 19600.00 10400.00 0.00 0.10659 19800.00 10400.00 0.00 0.10678 19400.00 10600.00 0.00 0.10749 19600.00 10600.00 0.00 0.10806 19800.00 10600.00 0.00 0.10825 19400.00 10800.00 0.00 0.10905 19600.00 10800.00 0.00 0.10963 19800.00 10800.00 0.00 0.10981 19400.00 11000.00 0.00 0.11080 19600.00 11000.00 0.00 0.11138 19800.00 11000.00 0.00 0.11156 19200.00 11200.00 0.00 0.11170 19400.00 11200.00 0.00 0.11286 19600.00 11200.00 0.00 0.11344 19800.00 11200.00 0.00 0.11360 19200.00 11400.00 0.00 0.11399 19400.00 11400.00 0.00 0.11514 19600.00 11400.00 0.00 0.11570 19800.00 11400.00 0.00 0.11583 19200.00 11600.00 0.00 0.11638 19400.00 11600.00 0.00 0.11751 19600.00 11600.00 0.00 0.11803 19800.00 11600.00 0.00 0.11813 19200.00 11800.00 0.00 0.11878 19400.00 11800.00 0.00 0.11987 19600.00 11800.00 0.00 0.12035 19800.00 11800.00 0.00 0.12041 19200.00 12000.00 0.00 0.12109 19400.00 12000.00 0.00 0.12213 19600.00 12000.00 0.00 0.12256 19800.00 12000.00 0.00 0.12256 19200.00 12200.00 0.00 0.12334 19400.00 12200.00 0.00 0.12434 19600.00 12200.00 0.00 0.12471 19200.00 12400.00 0.00 0.12574 19400.00 12400.00 0.00 0.12668 19600.00 12400.00 0.00 0.12701 19200.00 12600.00 0.00 0.12828 19400.00 12600.00 0.00 0.12918 19600.00 12600.00 0.00 0.12945 19200.00 12800.00 0.00 0.13084 19400.00 12800.00 0.00 0.13170 19600.00 12800.00 0.00 0.13196 19200.00 13000.00 0.00 0.13341 19400.00 13000.00 0.00 0.13426 19600.00 13000.00 0.00 0.13452 19200.00 13200.00 0.00 0.13597 19400.00 13200.00 0.00 0.13683 19600.00 13200.00 0.00 0.13711 19200.00 13400.00 0.00 0.13855 19400.00 13400.00 0.00 0.13945 19600.00 13400.00 0.00 0.13975 19200.00 13600.00 0.00 0.14142 19400.00 13600.00 0.00 0.14233 19600.00 13600.00 0.00 0.14264 19000.00 13800.00 0.00 0.14293 19200.00 13800.00 0.00 0.14466 19400.00 13800.00 0.00 0.14558 19600.00 13800.00 0.00 0.14586 19000.00 14000.00 0.00 0.14637 19200.00 14000.00 0.00 0.14811 19400.00 14000.00 0.00 0.14901 19600.00 14000.00 0.00 0.14925 19000.00 14200.00 0.00 0.14987 19200.00 14200.00 0.00 0.15160 19400.00 14200.00 0.00 0.15246 19600.00 14200.00 0.00 0.15265 19000.00 14400.00 0.00 0.15331 19200.00 14400.00 0.00 0.15501 19400.00 14400.00 0.00 0.15582 19600.00 14400.00 0.00 0.15593 19000.00 14600.00 0.00 0.15657 19200.00 14600.00 0.00 0.15822 19400.00 14600.00 0.00 0.15896 19600.00 14600.00 0.00 0.15899 19000.00 14800.00 0.00 0.15980 19200.00 14800.00 0.00 0.16141 19400.00 14800.00 0.00 0.16208 19000.00 15000.00 0.00 0.16329 19200.00 15000.00 0.00 0.16485 19400.00 15000.00 0.00 0.16546 19000.00 15200.00 0.00 0.16702 19200.00 15200.00 0.00 0.16853 19400.00 15200.00 0.00 0.16906 19000.00 15400.00 0.00 0.17082 19200.00 15400.00 0.00 0.17228 19400.00 15400.00 0.00 0.17274 19000.00 15600.00 0.00 0.17463 19200.00 15600.00 0.00 0.17604 19400.00 15600.00 0.00 0.17642 19000.00 15800.00 0.00 0.17831 19200.00 15800.00 0.00 0.17967 19400.00 15800.00 0.00 0.17998 19000.00 16000.00 0.00 0.18192 19200.00 16000.00 0.00 0.18321 19400.00 16000.00 0.00 0.18342 19000.00 16200.00 0.00 0.18578 19200.00 16200.00 0.00 0.18697 19400.00 16200.00 0.00 0.18706 18800.00 16400.00 0.00 0.18761 19000.00 16400.00 0.00 0.18995 19200.00 16400.00 0.00 0.19102 18800.00 16600.00 0.00 0.19199 19000.00 16600.00 0.00 0.19423 19200.00 16600.00 0.00 0.19517 18800.00 16800.00 0.00 0.19634 19000.00 16800.00 0.00 0.19847 19200.00 16800.00 0.00 0.19926 18800.00 17000.00 0.00 0.20050 19000.00 17000.00 0.00 0.20251 19200.00 17000.00 0.00 0.20314 18800.00 17200.00 0.00 0.20440 19000.00 17200.00 0.00 0.20629 19200.00 17200.00 0.00 0.20676 18800.00 17400.00 0.00 0.20846 19000.00 17400.00 0.00 0.21019 19200.00 17400.00 0.00 0.21044 18800.00 17600.00 0.00 0.21302 19000.00 17600.00 0.00 0.21451 18600.00 17800.00 0.00 0.21521 18800.00 17800.00 0.00 0.21787 19000.00 17800.00 0.00 0.21903 18600.00 18000.00 0.00 0.22038 18800.00 18000.00 0.00 0.22263 19000.00 18000.00 0.00 0.22336 18600.00 18200.00 0.00 0.22509 18800.00 18200.00 0.00 0.22679 19000.00 18200.00 0.00 0.22700 18600.00 18400.00 0.00 0.22841 18800.00 18400.00 0.00 0.22951 18600.00 18600.00 0.00 0.22995 18800.00 18600.00 0.00 0.23051 18600.00 18800.00 30.00 0.23052 18600.00 18800.00 35.00 0.23057 18600.00 18800.00 40.00 0.23062 18600.00 18800.00 45.00 0.23065 18600.00 18800.00 50.00 0.23068 18600.00 18800.00 55.00 0.23070 18600.00 18800.00 60.00 0.23071 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18633.33 18870.39 54.68 0.23081 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/368.ctf NX, NY, NZ: 360 360 1 MODE : real