CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/401.mrc Output diagnostic file name Micrographs/401.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/401.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2478.000 7942.000 Mean, RMS : 4408.488 224.4973 TITLE 1: emi2mrc: 401.emi Mon Jan 20 11:26:20 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X -450.519, Y 500.523, Z -26.464, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01491 8800.00 8000.00 0.00 -0.01487 9000.00 8000.00 0.00 -0.01467 9200.00 8000.00 0.00 -0.01443 9400.00 8000.00 0.00 -0.01411 9600.00 8000.00 0.00 -0.01365 9800.00 8000.00 0.00 -0.01308 10000.00 8000.00 0.00 -0.01250 10200.00 8000.00 0.00 -0.01194 10400.00 8000.00 0.00 -0.01136 10600.00 8000.00 0.00 -0.01073 10800.00 8000.00 0.00 -0.00999 11000.00 8000.00 0.00 -0.00914 11200.00 8000.00 0.00 -0.00826 11400.00 8000.00 0.00 -0.00741 11600.00 8000.00 0.00 -0.00663 11800.00 8000.00 0.00 -0.00588 12000.00 8000.00 0.00 -0.00509 12200.00 8000.00 0.00 -0.00417 12400.00 8000.00 0.00 -0.00308 12600.00 8000.00 0.00 -0.00184 12800.00 8000.00 0.00 -0.00047 13000.00 8000.00 0.00 0.00101 13200.00 8000.00 0.00 0.00257 13400.00 8000.00 0.00 0.00421 13600.00 8000.00 0.00 0.00589 13800.00 8000.00 0.00 0.00755 14000.00 8000.00 0.00 0.00917 14200.00 8000.00 0.00 0.01075 14400.00 8000.00 0.00 0.01234 14600.00 8000.00 0.00 0.01404 14800.00 8000.00 0.00 0.01592 15000.00 8000.00 0.00 0.01799 15200.00 8000.00 0.00 0.02019 15400.00 8000.00 0.00 0.02244 15600.00 8000.00 0.00 0.02470 15800.00 8000.00 0.00 0.02695 16000.00 8000.00 0.00 0.02923 16200.00 8000.00 0.00 0.03161 16400.00 8000.00 0.00 0.03413 16600.00 8000.00 0.00 0.03678 16800.00 8000.00 0.00 0.03950 17000.00 8000.00 0.00 0.04228 17200.00 8000.00 0.00 0.04509 17400.00 8000.00 0.00 0.04794 17600.00 8000.00 0.00 0.05088 17800.00 8000.00 0.00 0.05392 18000.00 8000.00 0.00 0.05707 18200.00 8000.00 0.00 0.06036 18400.00 8000.00 0.00 0.06382 18600.00 8000.00 0.00 0.06746 18800.00 8000.00 0.00 0.07123 19000.00 8000.00 0.00 0.07499 19200.00 8000.00 0.00 0.07844 19400.00 8000.00 0.00 0.08132 19600.00 8000.00 0.00 0.08341 19800.00 8000.00 0.00 0.08469 20000.00 8000.00 0.00 0.08528 20200.00 8000.00 0.00 0.08540 19800.00 8200.00 0.00 0.08570 20000.00 8200.00 0.00 0.08629 20200.00 8200.00 0.00 0.08640 19800.00 8400.00 0.00 0.08670 20000.00 8400.00 0.00 0.08729 20200.00 8400.00 0.00 0.08739 19800.00 8600.00 0.00 0.08785 20000.00 8600.00 0.00 0.08842 20200.00 8600.00 0.00 0.08850 19800.00 8800.00 0.00 0.08908 20000.00 8800.00 0.00 0.08962 20200.00 8800.00 0.00 0.08966 19800.00 9000.00 0.00 0.09025 20000.00 9000.00 0.00 0.09075 19800.00 9200.00 0.00 0.09132 20000.00 9200.00 0.00 0.09178 19800.00 9400.00 0.00 0.09230 20000.00 9400.00 0.00 0.09273 19800.00 9600.00 0.00 0.09332 20000.00 9600.00 0.00 0.09373 19800.00 9800.00 0.00 0.09461 20000.00 9800.00 0.00 0.09501 19600.00 10000.00 0.00 0.09504 19800.00 10000.00 0.00 0.09625 20000.00 10000.00 0.00 0.09664 19600.00 10200.00 0.00 0.09687 19800.00 10200.00 0.00 0.09808 20000.00 10200.00 0.00 0.09847 19600.00 10400.00 0.00 0.09872 19800.00 10400.00 0.00 0.09992 20000.00 10400.00 0.00 0.10030 19600.00 10600.00 0.00 0.10044 19800.00 10600.00 0.00 0.10163 20000.00 10600.00 0.00 0.10200 19800.00 10800.00 0.00 0.10315 20000.00 10800.00 0.00 0.10349 19800.00 11000.00 0.00 0.10462 20000.00 11000.00 0.00 0.10494 19600.00 11200.00 0.00 0.10509 19800.00 11200.00 0.00 0.10625 20000.00 11200.00 0.00 0.10655 19600.00 11400.00 0.00 0.10692 19800.00 11400.00 0.00 0.10808 20000.00 11400.00 0.00 0.10837 19600.00 11600.00 0.00 0.10893 19800.00 11600.00 0.00 0.11009 20000.00 11600.00 0.00 0.11037 19600.00 11800.00 0.00 0.11109 19800.00 11800.00 0.00 0.11226 20000.00 11800.00 0.00 0.11253 19600.00 12000.00 0.00 0.11331 19800.00 12000.00 0.00 0.11449 20000.00 12000.00 0.00 0.11475 19600.00 12200.00 0.00 0.11560 19800.00 12200.00 0.00 0.11678 20000.00 12200.00 0.00 0.11702 19600.00 12400.00 0.00 0.11806 19800.00 12400.00 0.00 0.11923 20000.00 12400.00 0.00 0.11943 19600.00 12600.00 0.00 0.12065 19800.00 12600.00 0.00 0.12180 20000.00 12600.00 0.00 0.12196 19600.00 12800.00 0.00 0.12324 19800.00 12800.00 0.00 0.12437 20000.00 12800.00 0.00 0.12449 19600.00 13000.00 0.00 0.12582 19800.00 13000.00 0.00 0.12693 20000.00 13000.00 0.00 0.12702 19600.00 13200.00 0.00 0.12838 19800.00 13200.00 0.00 0.12948 20000.00 13200.00 0.00 0.12952 19600.00 13400.00 0.00 0.13095 19800.00 13400.00 0.00 0.13203 19600.00 13600.00 0.00 0.13374 19800.00 13600.00 0.00 0.13480 19600.00 13800.00 0.00 0.13687 19800.00 13800.00 0.00 0.13790 19600.00 14000.00 0.00 0.14020 19800.00 14000.00 0.00 0.14121 19400.00 14200.00 0.00 0.14123 19600.00 14200.00 0.00 0.14365 19800.00 14200.00 0.00 0.14462 19400.00 14400.00 0.00 0.14472 19600.00 14400.00 0.00 0.14714 19800.00 14400.00 0.00 0.14805 19400.00 14600.00 0.00 0.14814 19600.00 14600.00 0.00 0.15053 19800.00 14600.00 0.00 0.15137 19400.00 14800.00 0.00 0.15161 19600.00 14800.00 0.00 0.15395 19800.00 14800.00 0.00 0.15471 19400.00 15000.00 0.00 0.15531 19600.00 15000.00 0.00 0.15760 19800.00 15000.00 0.00 0.15826 19400.00 15200.00 0.00 0.15918 19600.00 15200.00 0.00 0.16140 19800.00 15200.00 0.00 0.16196 19400.00 15400.00 0.00 0.16307 19600.00 15400.00 0.00 0.16523 19800.00 15400.00 0.00 0.16567 19400.00 15600.00 0.00 0.16699 19600.00 15600.00 0.00 0.16908 19800.00 15600.00 0.00 0.16941 19400.00 15800.00 0.00 0.17085 19600.00 15800.00 0.00 0.17286 19800.00 15800.00 0.00 0.17306 19400.00 16000.00 0.00 0.17467 19600.00 16000.00 0.00 0.17659 19800.00 16000.00 0.00 0.17666 19400.00 16200.00 0.00 0.17869 19600.00 16200.00 0.00 0.18051 19400.00 16400.00 0.00 0.18295 19600.00 16400.00 0.00 0.18466 19400.00 16600.00 0.00 0.18728 19600.00 16600.00 0.00 0.18888 19400.00 16800.00 0.00 0.19164 19600.00 16800.00 0.00 0.19312 19400.00 17000.00 0.00 0.19599 19600.00 17000.00 0.00 0.19734 19400.00 17200.00 0.00 0.20029 19600.00 17200.00 0.00 0.20149 19200.00 17400.00 0.00 0.20158 19400.00 17400.00 0.00 0.20476 19600.00 17400.00 0.00 0.20579 19200.00 17600.00 0.00 0.20651 19400.00 17600.00 0.00 0.20957 19600.00 17600.00 0.00 0.21041 19200.00 17800.00 0.00 0.21165 19400.00 17800.00 0.00 0.21458 19600.00 17800.00 0.00 0.21520 19200.00 18000.00 0.00 0.21697 19400.00 18000.00 0.00 0.21972 19600.00 18000.00 0.00 0.22007 19200.00 18200.00 0.00 0.22251 19400.00 18200.00 0.00 0.22503 19200.00 18400.00 0.00 0.22809 19400.00 18400.00 0.00 0.23028 19200.00 18600.00 0.00 0.23359 19400.00 18600.00 0.00 0.23534 19200.00 18800.00 0.00 0.23890 19400.00 18800.00 0.00 0.24002 19200.00 19000.00 0.00 0.24301 19400.00 19000.00 0.00 0.24331 19200.00 19200.00 0.00 0.24467 19200.00 19400.00 45.00 0.24473 19200.00 19400.00 50.00 0.24476 19200.00 19400.00 55.00 0.24477 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19137.88 19331.28 55.00 0.24518 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/401.ctf NX, NY, NZ: 360 360 1 MODE : real