CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/404.mrc Output diagnostic file name Micrographs/404.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/404.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1992.000 7546.000 Mean, RMS : 4307.778 215.2139 TITLE 1: emi2mrc: 404.emi Mon Jan 20 11:28:55 2014 TITLE 2: kV 300, Mag 78000x, Def 19850, Spot 4, Int 55.10%, Obj 88.07%, Dif 73.67% TITLE 3: X -444.065, Y 508.813, Z -26.464, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.03739 13600.00 8000.00 0.00 -0.03734 13800.00 8000.00 0.00 -0.03707 14000.00 8000.00 0.00 -0.03678 14200.00 8000.00 0.00 -0.03645 14400.00 8000.00 0.00 -0.03605 14600.00 8000.00 0.00 -0.03553 14800.00 8000.00 0.00 -0.03487 15000.00 8000.00 0.00 -0.03408 15200.00 8000.00 0.00 -0.03321 15400.00 8000.00 0.00 -0.03224 15600.00 8000.00 0.00 -0.03116 15800.00 8000.00 0.00 -0.02993 16000.00 8000.00 0.00 -0.02854 16200.00 8000.00 0.00 -0.02704 16400.00 8000.00 0.00 -0.02554 16600.00 8000.00 0.00 -0.02409 16800.00 8000.00 0.00 -0.02276 17000.00 8000.00 0.00 -0.02156 17200.00 8000.00 0.00 -0.02043 17400.00 8000.00 0.00 -0.01934 17600.00 8000.00 0.00 -0.01823 17800.00 8000.00 0.00 -0.01705 18000.00 8000.00 0.00 -0.01572 18200.00 8000.00 0.00 -0.01421 18400.00 8000.00 0.00 -0.01249 18600.00 8000.00 0.00 -0.01057 18800.00 8000.00 0.00 -0.00852 19000.00 8000.00 0.00 -0.00640 19200.00 8000.00 0.00 -0.00428 19400.00 8000.00 0.00 -0.00221 19600.00 8000.00 0.00 -0.00024 19800.00 8000.00 0.00 0.00161 20000.00 8000.00 0.00 0.00335 20200.00 8000.00 0.00 0.00500 20400.00 8000.00 0.00 0.00663 20600.00 8000.00 0.00 0.00829 20800.00 8000.00 0.00 0.01003 21000.00 8000.00 0.00 0.01186 21200.00 8000.00 0.00 0.01379 21400.00 8000.00 0.00 0.01581 21600.00 8000.00 0.00 0.01797 21800.00 8000.00 0.00 0.02028 22000.00 8000.00 0.00 0.02277 22200.00 8000.00 0.00 0.02541 22400.00 8000.00 0.00 0.02812 22600.00 8000.00 0.00 0.03085 22800.00 8000.00 0.00 0.03357 23000.00 8000.00 0.00 0.03629 23200.00 8000.00 0.00 0.03909 23400.00 8000.00 0.00 0.04202 23600.00 8000.00 0.00 0.04499 23800.00 8000.00 0.00 0.04786 24000.00 8000.00 0.00 0.05039 24200.00 8000.00 0.00 0.05232 24400.00 8000.00 0.00 0.05356 24600.00 8000.00 0.00 0.05414 24800.00 8000.00 0.00 0.05420 24600.00 8200.00 0.00 0.05448 24800.00 8200.00 0.00 0.05451 24600.00 8400.00 0.00 0.05487 24800.00 8400.00 0.00 0.05487 24600.00 8600.00 0.00 0.05538 24400.00 8800.00 0.00 0.05553 24600.00 8800.00 0.00 0.05603 24400.00 9000.00 0.00 0.05629 24600.00 9000.00 0.00 0.05677 24400.00 9200.00 0.00 0.05712 24600.00 9200.00 0.00 0.05757 24400.00 9400.00 0.00 0.05800 24600.00 9400.00 0.00 0.05840 24400.00 9600.00 0.00 0.05891 24600.00 9600.00 0.00 0.05927 24400.00 9800.00 0.00 0.05991 24600.00 9800.00 0.00 0.06022 24400.00 10000.00 0.00 0.06097 24600.00 10000.00 0.00 0.06123 24400.00 10200.00 0.00 0.06198 24600.00 10200.00 0.00 0.06218 24400.00 10400.00 0.00 0.06285 24600.00 10400.00 0.00 0.06300 24400.00 10600.00 0.00 0.06355 24600.00 10600.00 0.00 0.06364 24400.00 10800.00 0.00 0.06410 24600.00 10800.00 0.00 0.06415 24400.00 11000.00 0.00 0.06464 24600.00 11000.00 0.00 0.06468 24400.00 11200.00 0.00 0.06532 24600.00 11200.00 0.00 0.06534 24200.00 11400.00 0.00 0.06537 24400.00 11400.00 0.00 0.06615 24200.00 11600.00 0.00 0.06634 24400.00 11600.00 0.00 0.06710 24200.00 11800.00 0.00 0.06740 24400.00 11800.00 0.00 0.06814 24200.00 12000.00 0.00 0.06849 24400.00 12000.00 0.00 0.06920 24200.00 12200.00 0.00 0.06960 24400.00 12200.00 0.00 0.07026 24200.00 12400.00 0.00 0.07080 24400.00 12400.00 0.00 0.07141 24200.00 12600.00 0.00 0.07208 24400.00 12600.00 0.00 0.07263 24200.00 12800.00 0.00 0.07337 24400.00 12800.00 0.00 0.07387 24200.00 13000.00 0.00 0.07467 24400.00 13000.00 0.00 0.07512 24200.00 13200.00 0.00 0.07594 24400.00 13200.00 0.00 0.07634 24200.00 13400.00 0.00 0.07715 24400.00 13400.00 0.00 0.07750 24200.00 13600.00 0.00 0.07836 24400.00 13600.00 0.00 0.07866 24200.00 13800.00 0.00 0.07960 24400.00 13800.00 0.00 0.07987 24200.00 14000.00 0.00 0.08085 24400.00 14000.00 0.00 0.08110 24200.00 14200.00 0.00 0.08209 24400.00 14200.00 0.00 0.08234 24200.00 14400.00 0.00 0.08337 24400.00 14400.00 0.00 0.08362 24200.00 14600.00 0.00 0.08469 24400.00 14600.00 0.00 0.08494 24200.00 14800.00 0.00 0.08610 24400.00 14800.00 0.00 0.08635 24000.00 15000.00 0.00 0.08646 24200.00 15000.00 0.00 0.08767 24400.00 15000.00 0.00 0.08792 24000.00 15200.00 0.00 0.08812 24200.00 15200.00 0.00 0.08932 24400.00 15200.00 0.00 0.08958 24000.00 15400.00 0.00 0.08977 24200.00 15400.00 0.00 0.09097 24400.00 15400.00 0.00 0.09121 24000.00 15600.00 0.00 0.09137 24200.00 15600.00 0.00 0.09254 24400.00 15600.00 0.00 0.09276 24000.00 15800.00 0.00 0.09287 24200.00 15800.00 0.00 0.09402 24400.00 15800.00 0.00 0.09421 24000.00 16000.00 0.00 0.09433 24200.00 16000.00 0.00 0.09546 24400.00 16000.00 0.00 0.09563 24000.00 16200.00 0.00 0.09591 24200.00 16200.00 0.00 0.09702 24400.00 16200.00 0.00 0.09718 24000.00 16400.00 0.00 0.09765 24200.00 16400.00 0.00 0.09875 24400.00 16400.00 0.00 0.09892 24000.00 16600.00 0.00 0.09952 24200.00 16600.00 0.00 0.10062 24400.00 16600.00 0.00 0.10080 24000.00 16800.00 0.00 0.10149 24200.00 16800.00 0.00 0.10260 24400.00 16800.00 0.00 0.10278 24000.00 17000.00 0.00 0.10352 24200.00 17000.00 0.00 0.10462 24400.00 17000.00 0.00 0.10479 24000.00 17200.00 0.00 0.10552 24200.00 17200.00 0.00 0.10660 24400.00 17200.00 0.00 0.10676 24000.00 17400.00 0.00 0.10759 24200.00 17400.00 0.00 0.10864 24400.00 17400.00 0.00 0.10877 24000.00 17600.00 0.00 0.10976 24200.00 17600.00 0.00 0.11078 24400.00 17600.00 0.00 0.11088 24000.00 17800.00 0.00 0.11195 24200.00 17800.00 0.00 0.11294 24400.00 17800.00 0.00 0.11299 24000.00 18000.00 0.00 0.11413 24200.00 18000.00 0.00 0.11509 24400.00 18000.00 0.00 0.11511 24000.00 18200.00 0.00 0.11629 24200.00 18200.00 0.00 0.11723 24000.00 18400.00 0.00 0.11839 24200.00 18400.00 0.00 0.11932 24000.00 18600.00 0.00 0.12051 24200.00 18600.00 0.00 0.12143 24000.00 18800.00 0.00 0.12274 24200.00 18800.00 0.00 0.12362 24000.00 19000.00 0.00 0.12496 24200.00 19000.00 0.00 0.12579 24000.00 19200.00 0.00 0.12706 24200.00 19200.00 0.00 0.12785 24000.00 19400.00 0.00 0.12907 24200.00 19400.00 0.00 0.12982 24000.00 19600.00 0.00 0.13100 24200.00 19600.00 0.00 0.13174 24000.00 19800.00 0.00 0.13294 24200.00 19800.00 0.00 0.13368 24000.00 20000.00 0.00 0.13513 24200.00 20000.00 0.00 0.13587 24000.00 20200.00 0.00 0.13759 24200.00 20200.00 0.00 0.13832 24000.00 20400.00 0.00 0.14014 24200.00 20400.00 0.00 0.14085 24000.00 20600.00 0.00 0.14268 24200.00 20600.00 0.00 0.14333 24000.00 20800.00 0.00 0.14511 24200.00 20800.00 0.00 0.14569 24000.00 21000.00 0.00 0.14738 24200.00 21000.00 0.00 0.14787 23800.00 21200.00 0.00 0.14792 24000.00 21200.00 0.00 0.14965 24200.00 21200.00 0.00 0.15002 23800.00 21400.00 0.00 0.15040 24000.00 21400.00 0.00 0.15204 24200.00 21400.00 0.00 0.15228 23800.00 21600.00 0.00 0.15290 24000.00 21600.00 0.00 0.15443 24200.00 21600.00 0.00 0.15454 23800.00 21800.00 0.00 0.15528 24000.00 21800.00 0.00 0.15670 23800.00 22000.00 0.00 0.15748 24000.00 22000.00 0.00 0.15878 23800.00 22200.00 0.00 0.15946 24000.00 22200.00 0.00 0.16063 23800.00 22400.00 0.00 0.16139 24000.00 22400.00 0.00 0.16244 23800.00 22600.00 0.00 0.16365 24000.00 22600.00 0.00 0.16451 23800.00 22800.00 0.00 0.16619 24000.00 22800.00 0.00 0.16681 23600.00 23000.00 0.00 0.16684 23800.00 23000.00 0.00 0.16875 24000.00 23000.00 0.00 0.16907 23600.00 23200.00 0.00 0.16953 23800.00 23200.00 0.00 0.17109 23600.00 23400.00 0.00 0.17159 23800.00 23400.00 0.00 0.17274 23800.00 23600.00 0.00 0.17334 23600.00 23800.00 45.00 0.17339 23600.00 23800.00 50.00 0.17345 23600.00 23800.00 55.00 0.17350 23600.00 23800.00 60.00 0.17352 23600.00 23800.00 65.00 0.17353 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 23564.16 23890.01 61.67 0.17362 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/404.ctf NX, NY, NZ: 360 360 1 MODE : real