CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/411.mrc Output diagnostic file name Micrographs/411.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/411.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2412.000 8804.000 Mean, RMS : 4387.287 213.8750 TITLE 1: emi2mrc: 411.emi Mon Jan 20 11:39:27 2014 TITLE 2: kV 300, Mag 78000x, Def 18870, Spot 4, Int 55.10%, Obj 88.09%, Dif 73.67% TITLE 3: X -457.933, Y 495.541, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 118 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.02101 8200.00 8000.00 0.00 0.02274 8400.00 8000.00 0.00 0.02425 8600.00 8000.00 0.00 0.02581 8800.00 8000.00 0.00 0.02774 9000.00 8000.00 0.00 0.03011 9200.00 8000.00 0.00 0.03282 9400.00 8000.00 0.00 0.03570 9600.00 8000.00 0.00 0.03861 9800.00 8000.00 0.00 0.04164 10000.00 8000.00 0.00 0.04510 10200.00 8000.00 0.00 0.04918 10400.00 8000.00 0.00 0.05377 10600.00 8000.00 0.00 0.05864 10800.00 8000.00 0.00 0.06350 11000.00 8000.00 0.00 0.06823 11200.00 8000.00 0.00 0.07311 11400.00 8000.00 0.00 0.07847 11600.00 8000.00 0.00 0.08436 11800.00 8000.00 0.00 0.09058 12000.00 8000.00 0.00 0.09676 12200.00 8000.00 0.00 0.10262 12400.00 8000.00 0.00 0.10834 12600.00 8000.00 0.00 0.11447 12800.00 8000.00 0.00 0.12138 13000.00 8000.00 0.00 0.12897 13200.00 8000.00 0.00 0.13656 13400.00 8000.00 0.00 0.14306 13600.00 8000.00 0.00 0.14762 13800.00 8000.00 0.00 0.15005 14000.00 8000.00 0.00 0.15074 13800.00 8200.00 0.00 0.15270 14000.00 8200.00 0.00 0.15333 13800.00 8400.00 0.00 0.15547 14000.00 8400.00 0.00 0.15605 13600.00 8600.00 0.00 0.15652 13800.00 8600.00 0.00 0.15882 14000.00 8600.00 0.00 0.15934 13600.00 8800.00 0.00 0.16049 13800.00 8800.00 0.00 0.16274 14000.00 8800.00 0.00 0.16318 13600.00 9000.00 0.00 0.16468 13800.00 9000.00 0.00 0.16686 14000.00 9000.00 0.00 0.16722 13600.00 9200.00 0.00 0.16885 13800.00 9200.00 0.00 0.17095 14000.00 9200.00 0.00 0.17121 13600.00 9400.00 0.00 0.17274 13800.00 9400.00 0.00 0.17475 14000.00 9400.00 0.00 0.17490 13600.00 9600.00 0.00 0.17633 13800.00 9600.00 0.00 0.17825 14000.00 9600.00 0.00 0.17828 13600.00 9800.00 0.00 0.18009 13800.00 9800.00 0.00 0.18190 13600.00 10000.00 0.00 0.18432 13800.00 10000.00 0.00 0.18601 13600.00 10200.00 0.00 0.18883 13800.00 10200.00 0.00 0.19038 13600.00 10400.00 0.00 0.19339 13800.00 10400.00 0.00 0.19478 13600.00 10600.00 0.00 0.19783 13800.00 10600.00 0.00 0.19905 13600.00 10800.00 0.00 0.20201 13800.00 10800.00 0.00 0.20304 13600.00 11000.00 0.00 0.20622 13800.00 11000.00 0.00 0.20705 13400.00 11200.00 0.00 0.20751 13600.00 11200.00 0.00 0.21092 13800.00 11200.00 0.00 0.21150 13400.00 11400.00 0.00 0.21275 13600.00 11400.00 0.00 0.21594 13800.00 11400.00 0.00 0.21623 13400.00 11600.00 0.00 0.21796 13600.00 11600.00 0.00 0.22089 13400.00 11800.00 0.00 0.22284 13600.00 11800.00 0.00 0.22550 13400.00 12000.00 0.00 0.22711 13600.00 12000.00 0.00 0.22948 13400.00 12200.00 0.00 0.23095 13600.00 12200.00 0.00 0.23305 13400.00 12400.00 0.00 0.23520 13600.00 12400.00 0.00 0.23694 13400.00 12600.00 0.00 0.24002 13600.00 12600.00 0.00 0.24127 13400.00 12800.00 0.00 0.24488 13600.00 12800.00 0.00 0.24550 13400.00 13000.00 0.00 0.24925 13400.00 13200.00 0.00 0.25233 13400.00 13400.00 0.00 0.25325 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13441.52 13347.51 -40.19 0.25335 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/411.ctf NX, NY, NZ: 360 360 1 MODE : real