CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/419.mrc Output diagnostic file name Micrographs/419.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/419.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2238.000 7938.000 Mean, RMS : 4325.821 219.8927 TITLE 1: emi2mrc: 419.emi Mon Jan 20 11:47:39 2014 TITLE 2: kV 300, Mag 78000x, Def 18870, Spot 4, Int 55.10%, Obj 88.09%, Dif 73.67% TITLE 3: X -446.282, Y 514.124, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01272 8200.00 8000.00 0.00 -0.01252 8400.00 8000.00 0.00 -0.01232 8600.00 8000.00 0.00 -0.01218 8800.00 8000.00 0.00 -0.01214 9800.00 8000.00 0.00 -0.01195 10000.00 8000.00 0.00 -0.01152 10200.00 8000.00 0.00 -0.01095 10400.00 8000.00 0.00 -0.01032 10600.00 8000.00 0.00 -0.00966 10800.00 8000.00 0.00 -0.00899 11000.00 8000.00 0.00 -0.00828 11200.00 8000.00 0.00 -0.00752 11400.00 8000.00 0.00 -0.00672 11600.00 8000.00 0.00 -0.00591 11800.00 8000.00 0.00 -0.00514 12000.00 8000.00 0.00 -0.00439 12200.00 8000.00 0.00 -0.00362 12400.00 8000.00 0.00 -0.00275 12600.00 8000.00 0.00 -0.00170 12800.00 8000.00 0.00 -0.00049 13000.00 8000.00 0.00 0.00083 13200.00 8000.00 0.00 0.00214 13400.00 8000.00 0.00 0.00339 13600.00 8000.00 0.00 0.00458 13800.00 8000.00 0.00 0.00580 14000.00 8000.00 0.00 0.00716 14200.00 8000.00 0.00 0.00870 14400.00 8000.00 0.00 0.01045 14600.00 8000.00 0.00 0.01237 14800.00 8000.00 0.00 0.01439 15000.00 8000.00 0.00 0.01650 15200.00 8000.00 0.00 0.01866 15400.00 8000.00 0.00 0.02083 15600.00 8000.00 0.00 0.02299 15800.00 8000.00 0.00 0.02515 16000.00 8000.00 0.00 0.02734 16200.00 8000.00 0.00 0.02964 16400.00 8000.00 0.00 0.03212 16600.00 8000.00 0.00 0.03475 16800.00 8000.00 0.00 0.03744 17000.00 8000.00 0.00 0.04006 17200.00 8000.00 0.00 0.04252 17400.00 8000.00 0.00 0.04482 17600.00 8000.00 0.00 0.04708 17800.00 8000.00 0.00 0.04939 18000.00 8000.00 0.00 0.05179 18200.00 8000.00 0.00 0.05428 18400.00 8000.00 0.00 0.05680 18600.00 8000.00 0.00 0.05938 18800.00 8000.00 0.00 0.06217 19000.00 8000.00 0.00 0.06530 19200.00 8000.00 0.00 0.06875 19400.00 8000.00 0.00 0.07237 19600.00 8000.00 0.00 0.07579 19800.00 8000.00 0.00 0.07865 20000.00 8000.00 0.00 0.08073 20200.00 8000.00 0.00 0.08206 20400.00 8000.00 0.00 0.08279 20600.00 8000.00 0.00 0.08319 20800.00 8000.00 0.00 0.08343 21000.00 8000.00 0.00 0.08353 20400.00 8200.00 0.00 0.08371 20600.00 8200.00 0.00 0.08412 20800.00 8200.00 0.00 0.08436 21000.00 8200.00 0.00 0.08443 20400.00 8400.00 0.00 0.08469 20600.00 8400.00 0.00 0.08510 20800.00 8400.00 0.00 0.08532 21000.00 8400.00 0.00 0.08534 20400.00 8600.00 0.00 0.08586 20600.00 8600.00 0.00 0.08625 20800.00 8600.00 0.00 0.08642 20400.00 8800.00 0.00 0.08709 20600.00 8800.00 0.00 0.08745 20800.00 8800.00 0.00 0.08758 20400.00 9000.00 0.00 0.08819 20600.00 9000.00 0.00 0.08851 20800.00 9000.00 0.00 0.08860 20400.00 9200.00 0.00 0.08908 20600.00 9200.00 0.00 0.08938 20800.00 9200.00 0.00 0.08945 20400.00 9400.00 0.00 0.08982 20600.00 9400.00 0.00 0.09010 20800.00 9400.00 0.00 0.09015 20400.00 9600.00 0.00 0.09053 20600.00 9600.00 0.00 0.09081 20800.00 9600.00 0.00 0.09085 20400.00 9800.00 0.00 0.09148 20600.00 9800.00 0.00 0.09176 20800.00 9800.00 0.00 0.09181 20200.00 10000.00 0.00 0.09207 20400.00 10000.00 0.00 0.09276 20600.00 10000.00 0.00 0.09305 20800.00 10000.00 0.00 0.09313 20200.00 10200.00 0.00 0.09353 20400.00 10200.00 0.00 0.09424 20600.00 10200.00 0.00 0.09456 20800.00 10200.00 0.00 0.09465 20200.00 10400.00 0.00 0.09504 20400.00 10400.00 0.00 0.09578 20600.00 10400.00 0.00 0.09613 20800.00 10400.00 0.00 0.09625 20200.00 10600.00 0.00 0.09655 20400.00 10600.00 0.00 0.09732 20600.00 10600.00 0.00 0.09769 20800.00 10600.00 0.00 0.09782 20200.00 10800.00 0.00 0.09804 20400.00 10800.00 0.00 0.09884 20600.00 10800.00 0.00 0.09922 20800.00 10800.00 0.00 0.09934 20200.00 11000.00 0.00 0.09966 20400.00 11000.00 0.00 0.10047 20600.00 11000.00 0.00 0.10085 20800.00 11000.00 0.00 0.10096 20200.00 11200.00 0.00 0.10155 20400.00 11200.00 0.00 0.10235 20600.00 11200.00 0.00 0.10272 20800.00 11200.00 0.00 0.10281 20200.00 11400.00 0.00 0.10361 20400.00 11400.00 0.00 0.10440 20600.00 11400.00 0.00 0.10475 20800.00 11400.00 0.00 0.10482 20200.00 11600.00 0.00 0.10570 20400.00 11600.00 0.00 0.10648 20600.00 11600.00 0.00 0.10680 20800.00 11600.00 0.00 0.10684 20200.00 11800.00 0.00 0.10773 20400.00 11800.00 0.00 0.10848 20600.00 11800.00 0.00 0.10878 20200.00 12000.00 0.00 0.10964 20400.00 12000.00 0.00 0.11037 20600.00 12000.00 0.00 0.11064 20200.00 12200.00 0.00 0.11155 20400.00 12200.00 0.00 0.11227 20600.00 12200.00 0.00 0.11251 20200.00 12400.00 0.00 0.11367 20400.00 12400.00 0.00 0.11439 20600.00 12400.00 0.00 0.11462 20200.00 12600.00 0.00 0.11602 20400.00 12600.00 0.00 0.11674 20600.00 12600.00 0.00 0.11696 20000.00 12800.00 0.00 0.11702 20200.00 12800.00 0.00 0.11849 20400.00 12800.00 0.00 0.11921 20600.00 12800.00 0.00 0.11941 20000.00 13000.00 0.00 0.11950 20200.00 13000.00 0.00 0.12098 20400.00 13000.00 0.00 0.12169 20600.00 13000.00 0.00 0.12187 20000.00 13200.00 0.00 0.12194 20200.00 13200.00 0.00 0.12343 20400.00 13200.00 0.00 0.12412 20600.00 13200.00 0.00 0.12425 20000.00 13400.00 0.00 0.12436 20200.00 13400.00 0.00 0.12584 20400.00 13400.00 0.00 0.12650 20600.00 13400.00 0.00 0.12658 20000.00 13600.00 0.00 0.12698 20200.00 13600.00 0.00 0.12843 20400.00 13600.00 0.00 0.12905 20600.00 13600.00 0.00 0.12907 20000.00 13800.00 0.00 0.12989 20200.00 13800.00 0.00 0.13131 20400.00 13800.00 0.00 0.13187 20000.00 14000.00 0.00 0.13294 20200.00 14000.00 0.00 0.13433 20400.00 14000.00 0.00 0.13482 20000.00 14200.00 0.00 0.13602 20200.00 14200.00 0.00 0.13734 20400.00 14200.00 0.00 0.13776 20000.00 14400.00 0.00 0.13903 20200.00 14400.00 0.00 0.14030 20400.00 14400.00 0.00 0.14063 20000.00 14600.00 0.00 0.14193 20200.00 14600.00 0.00 0.14315 20400.00 14600.00 0.00 0.14343 20000.00 14800.00 0.00 0.14492 20200.00 14800.00 0.00 0.14610 20400.00 14800.00 0.00 0.14633 20000.00 15000.00 0.00 0.14823 20200.00 15000.00 0.00 0.14938 20400.00 15000.00 0.00 0.14956 20000.00 15200.00 0.00 0.15178 20200.00 15200.00 0.00 0.15287 20400.00 15200.00 0.00 0.15299 19800.00 15400.00 0.00 0.15310 20000.00 15400.00 0.00 0.15538 20200.00 15400.00 0.00 0.15642 20400.00 15400.00 0.00 0.15646 19800.00 15600.00 0.00 0.15673 20000.00 15600.00 0.00 0.15897 20200.00 15600.00 0.00 0.15994 19800.00 15800.00 0.00 0.16029 20000.00 15800.00 0.00 0.16248 20200.00 15800.00 0.00 0.16336 19800.00 16000.00 0.00 0.16386 20000.00 16000.00 0.00 0.16596 20200.00 16000.00 0.00 0.16674 19800.00 16200.00 0.00 0.16769 20000.00 16200.00 0.00 0.16970 20200.00 16200.00 0.00 0.17036 19800.00 16400.00 0.00 0.17177 20000.00 16400.00 0.00 0.17367 20200.00 16400.00 0.00 0.17420 19800.00 16600.00 0.00 0.17581 20000.00 16600.00 0.00 0.17760 20200.00 16600.00 0.00 0.17799 19800.00 16800.00 0.00 0.17964 20000.00 16800.00 0.00 0.18132 20200.00 16800.00 0.00 0.18160 19800.00 17000.00 0.00 0.18324 20000.00 17000.00 0.00 0.18483 20200.00 17000.00 0.00 0.18501 19800.00 17200.00 0.00 0.18672 20000.00 17200.00 0.00 0.18825 20200.00 17200.00 0.00 0.18836 19800.00 17400.00 0.00 0.19055 20000.00 17400.00 0.00 0.19202 20200.00 17400.00 0.00 0.19204 19800.00 17600.00 0.00 0.19500 20000.00 17600.00 0.00 0.19640 19600.00 17800.00 0.00 0.19665 19800.00 17800.00 0.00 0.19982 20000.00 17800.00 0.00 0.20112 19600.00 18000.00 0.00 0.20149 19800.00 18000.00 0.00 0.20462 20000.00 18000.00 0.00 0.20578 19600.00 18200.00 0.00 0.20607 19800.00 18200.00 0.00 0.20912 20000.00 18200.00 0.00 0.21010 19600.00 18400.00 0.00 0.21024 19800.00 18400.00 0.00 0.21320 20000.00 18400.00 0.00 0.21398 19600.00 18600.00 0.00 0.21446 19800.00 18600.00 0.00 0.21728 20000.00 18600.00 0.00 0.21777 19600.00 18800.00 0.00 0.21941 19800.00 18800.00 0.00 0.22195 20000.00 18800.00 0.00 0.22201 19600.00 19000.00 0.00 0.22498 19800.00 19000.00 0.00 0.22705 19600.00 19200.00 0.00 0.23037 19800.00 19200.00 0.00 0.23176 19600.00 19400.00 0.00 0.23467 19800.00 19400.00 0.00 0.23514 19600.00 19600.00 0.00 0.23663 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19715.76 19547.24 -34.93 0.23715 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/419.ctf NX, NY, NZ: 360 360 1 MODE : real