CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/423.mrc Output diagnostic file name Micrographs/423.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/423.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2122.000 7854.000 Mean, RMS : 4447.897 212.4338 TITLE 1: emi2mrc: 423.emi Mon Jan 20 11:55:54 2014 TITLE 2: kV 300, Mag 78000x, Def 18050, Spot 4, Int 55.08%, Obj 88.09%, Dif 73.67% TITLE 3: X -450.703, Y 487.220, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.14099 8200.00 8000.00 0.00 0.15130 8400.00 8000.00 0.00 0.16087 8600.00 8000.00 0.00 0.17108 8800.00 8000.00 0.00 0.18337 9000.00 8000.00 0.00 0.19752 9200.00 8000.00 0.00 0.21205 9400.00 8000.00 0.00 0.22481 9600.00 8000.00 0.00 0.23336 9800.00 8000.00 0.00 0.23665 9600.00 8200.00 0.00 0.24132 9800.00 8200.00 0.00 0.24414 9600.00 8400.00 0.00 0.24937 9800.00 8400.00 0.00 0.25167 9600.00 8600.00 0.00 0.25878 9800.00 8600.00 0.00 0.26035 9400.00 8800.00 0.00 0.26253 9600.00 8800.00 0.00 0.26949 9800.00 8800.00 0.00 0.27002 9400.00 9000.00 0.00 0.27418 9600.00 9000.00 0.00 0.28006 9400.00 9200.00 0.00 0.28469 9600.00 9200.00 0.00 0.28906 9400.00 9400.00 0.00 0.29225 9600.00 9400.00 0.00 0.29467 9400.00 9600.00 0.00 0.29506 9600.00 9600.00 0.00 0.29546 9400.00 9600.00 10.00 0.29551 9400.00 9600.00 15.00 0.29571 9400.00 9600.00 20.00 0.29588 9400.00 9600.00 25.00 0.29602 9400.00 9600.00 30.00 0.29612 9400.00 9600.00 35.00 0.29618 9400.00 9600.00 40.00 0.29620 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 9475.39 9593.83 33.83 0.29659 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/423.ctf NX, NY, NZ: 360 360 1 MODE : real