CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/431.mrc Output diagnostic file name Micrographs/431.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/431.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2352.000 7846.000 Mean, RMS : 4471.065 226.1918 TITLE 1: emi2mrc: 431.emi Mon Jan 20 12:05:01 2014 TITLE 2: kV 300, Mag 78000x, Def 16900, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X -434.098, Y 503.396, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01401 8200.00 8000.00 0.00 -0.01386 8400.00 8000.00 0.00 -0.01366 8600.00 8000.00 0.00 -0.01352 8800.00 8000.00 0.00 -0.01348 9000.00 8000.00 0.00 -0.01346 9200.00 8000.00 0.00 -0.01338 9400.00 8000.00 0.00 -0.01316 9600.00 8000.00 0.00 -0.01275 9800.00 8000.00 0.00 -0.01220 10000.00 8000.00 0.00 -0.01162 10200.00 8000.00 0.00 -0.01105 10400.00 8000.00 0.00 -0.01050 10600.00 8000.00 0.00 -0.00993 10800.00 8000.00 0.00 -0.00928 11000.00 8000.00 0.00 -0.00852 11200.00 8000.00 0.00 -0.00764 11400.00 8000.00 0.00 -0.00664 11600.00 8000.00 0.00 -0.00553 11800.00 8000.00 0.00 -0.00432 12000.00 8000.00 0.00 -0.00302 12200.00 8000.00 0.00 -0.00165 12400.00 8000.00 0.00 -0.00029 12600.00 8000.00 0.00 0.00105 12800.00 8000.00 0.00 0.00233 13000.00 8000.00 0.00 0.00360 13200.00 8000.00 0.00 0.00489 13400.00 8000.00 0.00 0.00625 13600.00 8000.00 0.00 0.00772 13800.00 8000.00 0.00 0.00931 14000.00 8000.00 0.00 0.01101 14200.00 8000.00 0.00 0.01280 14400.00 8000.00 0.00 0.01468 14600.00 8000.00 0.00 0.01661 14800.00 8000.00 0.00 0.01861 15000.00 8000.00 0.00 0.02068 15200.00 8000.00 0.00 0.02285 15400.00 8000.00 0.00 0.02515 15600.00 8000.00 0.00 0.02761 15800.00 8000.00 0.00 0.03021 16000.00 8000.00 0.00 0.03289 16200.00 8000.00 0.00 0.03558 16400.00 8000.00 0.00 0.03823 16600.00 8000.00 0.00 0.04081 16800.00 8000.00 0.00 0.04338 17000.00 8000.00 0.00 0.04605 17200.00 8000.00 0.00 0.04889 17400.00 8000.00 0.00 0.05194 17600.00 8000.00 0.00 0.05520 17800.00 8000.00 0.00 0.05862 18000.00 8000.00 0.00 0.06219 18200.00 8000.00 0.00 0.06595 18400.00 8000.00 0.00 0.06996 18600.00 8000.00 0.00 0.07423 18800.00 8000.00 0.00 0.07863 19000.00 8000.00 0.00 0.08283 19200.00 8000.00 0.00 0.08640 19400.00 8000.00 0.00 0.08897 19600.00 8000.00 0.00 0.09043 19800.00 8000.00 0.00 0.09096 19600.00 8200.00 0.00 0.09148 19800.00 8200.00 0.00 0.09196 19600.00 8400.00 0.00 0.09260 19800.00 8400.00 0.00 0.09304 19600.00 8600.00 0.00 0.09396 19800.00 8600.00 0.00 0.09435 19600.00 8800.00 0.00 0.09548 19800.00 8800.00 0.00 0.09582 19600.00 9000.00 0.00 0.09698 19800.00 9000.00 0.00 0.09727 19600.00 9200.00 0.00 0.09837 19800.00 9200.00 0.00 0.09860 19600.00 9400.00 0.00 0.09956 19800.00 9400.00 0.00 0.09976 19600.00 9600.00 0.00 0.10062 19800.00 9600.00 0.00 0.10079 19600.00 9800.00 0.00 0.10176 19800.00 9800.00 0.00 0.10192 19400.00 10000.00 0.00 0.10204 19600.00 10000.00 0.00 0.10313 19800.00 10000.00 0.00 0.10329 19400.00 10200.00 0.00 0.10359 19600.00 10200.00 0.00 0.10468 19800.00 10200.00 0.00 0.10483 19400.00 10400.00 0.00 0.10526 19600.00 10400.00 0.00 0.10635 19800.00 10400.00 0.00 0.10649 19400.00 10600.00 0.00 0.10702 19600.00 10600.00 0.00 0.10810 19800.00 10600.00 0.00 0.10824 19400.00 10800.00 0.00 0.10881 19600.00 10800.00 0.00 0.10989 19800.00 10800.00 0.00 0.11001 19400.00 11000.00 0.00 0.11072 19600.00 11000.00 0.00 0.11179 19800.00 11000.00 0.00 0.11189 19400.00 11200.00 0.00 0.11281 19600.00 11200.00 0.00 0.11386 19800.00 11200.00 0.00 0.11393 19400.00 11400.00 0.00 0.11497 19600.00 11400.00 0.00 0.11599 19800.00 11400.00 0.00 0.11602 19400.00 11600.00 0.00 0.11707 19600.00 11600.00 0.00 0.11805 19400.00 11800.00 0.00 0.11907 19600.00 11800.00 0.00 0.12002 19400.00 12000.00 0.00 0.12095 19600.00 12000.00 0.00 0.12188 19400.00 12200.00 0.00 0.12287 19600.00 12200.00 0.00 0.12378 19400.00 12400.00 0.00 0.12508 19600.00 12400.00 0.00 0.12597 19400.00 12600.00 0.00 0.12759 19600.00 12600.00 0.00 0.12846 19400.00 12800.00 0.00 0.13029 19600.00 12800.00 0.00 0.13112 19400.00 13000.00 0.00 0.13309 19600.00 13000.00 0.00 0.13387 19400.00 13200.00 0.00 0.13587 19600.00 13200.00 0.00 0.13661 19400.00 13400.00 0.00 0.13858 19600.00 13400.00 0.00 0.13926 19200.00 13600.00 0.00 0.13938 19400.00 13600.00 0.00 0.14137 19600.00 13600.00 0.00 0.14198 19200.00 13800.00 0.00 0.14240 19400.00 13800.00 0.00 0.14434 19600.00 13800.00 0.00 0.14489 19200.00 14000.00 0.00 0.14550 19400.00 14000.00 0.00 0.14740 19600.00 14000.00 0.00 0.14790 19200.00 14200.00 0.00 0.14864 19400.00 14200.00 0.00 0.15052 19600.00 14200.00 0.00 0.15100 19200.00 14400.00 0.00 0.15186 19400.00 14400.00 0.00 0.15372 19600.00 14400.00 0.00 0.15418 19200.00 14600.00 0.00 0.15510 19400.00 14600.00 0.00 0.15695 19600.00 14600.00 0.00 0.15738 19200.00 14800.00 0.00 0.15849 19400.00 14800.00 0.00 0.16032 19600.00 14800.00 0.00 0.16072 19200.00 15000.00 0.00 0.16219 19400.00 15000.00 0.00 0.16399 19600.00 15000.00 0.00 0.16435 19200.00 15200.00 0.00 0.16603 19400.00 15200.00 0.00 0.16780 19600.00 15200.00 0.00 0.16810 19200.00 15400.00 0.00 0.16980 19400.00 15400.00 0.00 0.17152 19600.00 15400.00 0.00 0.17175 19200.00 15600.00 0.00 0.17348 19400.00 15600.00 0.00 0.17515 19600.00 15600.00 0.00 0.17529 19200.00 15800.00 0.00 0.17704 19400.00 15800.00 0.00 0.17864 19600.00 15800.00 0.00 0.17869 19200.00 16000.00 0.00 0.18059 19400.00 16000.00 0.00 0.18212 19200.00 16200.00 0.00 0.18446 19400.00 16200.00 0.00 0.18589 19200.00 16400.00 0.00 0.18866 19400.00 16400.00 0.00 0.18997 19000.00 16600.00 0.00 0.19000 19200.00 16600.00 0.00 0.19291 19400.00 16600.00 0.00 0.19409 19000.00 16800.00 0.00 0.19424 19200.00 16800.00 0.00 0.19704 19400.00 16800.00 0.00 0.19808 19000.00 17000.00 0.00 0.19826 19200.00 17000.00 0.00 0.20096 19400.00 17000.00 0.00 0.20186 19000.00 17200.00 0.00 0.20207 19200.00 17200.00 0.00 0.20467 19400.00 17200.00 0.00 0.20542 19000.00 17400.00 0.00 0.20607 19200.00 17400.00 0.00 0.20856 19400.00 17400.00 0.00 0.20914 19000.00 17600.00 0.00 0.21054 19200.00 17600.00 0.00 0.21286 19400.00 17600.00 0.00 0.21323 19000.00 17800.00 0.00 0.21519 19200.00 17800.00 0.00 0.21730 19400.00 17800.00 0.00 0.21741 19000.00 18000.00 0.00 0.21969 19200.00 18000.00 0.00 0.22152 19000.00 18200.00 0.00 0.22369 19200.00 18200.00 0.00 0.22519 19000.00 18400.00 0.00 0.22673 19200.00 18400.00 0.00 0.22786 19000.00 18600.00 0.00 0.22883 19200.00 18600.00 0.00 0.22959 19000.00 18800.00 0.00 0.23046 19200.00 18800.00 0.00 0.23078 19000.00 19000.00 0.00 0.23146 19000.00 19200.00 10.00 0.23149 19000.00 19200.00 15.00 0.23158 19000.00 19200.00 20.00 0.23166 19000.00 19200.00 25.00 0.23173 19000.00 19200.00 30.00 0.23179 19000.00 19200.00 35.00 0.23183 19000.00 19200.00 40.00 0.23185 19000.00 19200.00 45.00 0.23185 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18959.70 19156.52 49.03 0.23195 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/431.ctf NX, NY, NZ: 360 360 1 MODE : real