CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/435.mrc Output diagnostic file name Micrographs/435.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/435.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2122.000 7376.000 Mean, RMS : 4473.549 222.7658 TITLE 1: emi2mrc: 435.emi Mon Jan 20 12:09:07 2014 TITLE 2: kV 300, Mag 78000x, Def 16900, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X -444.283, Y 491.101, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.01660 8200.00 8000.00 0.00 0.01773 8400.00 8000.00 0.00 0.01909 8600.00 8000.00 0.00 0.02069 8800.00 8000.00 0.00 0.02244 9000.00 8000.00 0.00 0.02414 9200.00 8000.00 0.00 0.02567 9400.00 8000.00 0.00 0.02713 9600.00 8000.00 0.00 0.02867 9800.00 8000.00 0.00 0.03050 10000.00 8000.00 0.00 0.03275 10200.00 8000.00 0.00 0.03538 10400.00 8000.00 0.00 0.03825 10600.00 8000.00 0.00 0.04126 10800.00 8000.00 0.00 0.04441 11000.00 8000.00 0.00 0.04770 11200.00 8000.00 0.00 0.05124 11400.00 8000.00 0.00 0.05504 11600.00 8000.00 0.00 0.05905 11800.00 8000.00 0.00 0.06325 12000.00 8000.00 0.00 0.06774 12200.00 8000.00 0.00 0.07255 12400.00 8000.00 0.00 0.07770 12600.00 8000.00 0.00 0.08311 12800.00 8000.00 0.00 0.08853 13000.00 8000.00 0.00 0.09378 13200.00 8000.00 0.00 0.09892 13400.00 8000.00 0.00 0.10422 13600.00 8000.00 0.00 0.11005 13800.00 8000.00 0.00 0.11656 14000.00 8000.00 0.00 0.12338 14200.00 8000.00 0.00 0.12968 14400.00 8000.00 0.00 0.13463 14600.00 8000.00 0.00 0.13765 14800.00 8000.00 0.00 0.13882 14600.00 8200.00 0.00 0.13996 14800.00 8200.00 0.00 0.14108 14600.00 8400.00 0.00 0.14219 14800.00 8400.00 0.00 0.14327 14600.00 8600.00 0.00 0.14472 14800.00 8600.00 0.00 0.14579 14600.00 8800.00 0.00 0.14758 14800.00 8800.00 0.00 0.14865 14600.00 9000.00 0.00 0.15061 14800.00 9000.00 0.00 0.15170 14600.00 9200.00 0.00 0.15377 14800.00 9200.00 0.00 0.15489 14600.00 9400.00 0.00 0.15703 14800.00 9400.00 0.00 0.15816 14600.00 9600.00 0.00 0.16047 14800.00 9600.00 0.00 0.16159 14600.00 9800.00 0.00 0.16442 14800.00 9800.00 0.00 0.16550 14400.00 10000.00 0.00 0.16579 14600.00 10000.00 0.00 0.16897 14800.00 10000.00 0.00 0.16999 14400.00 10200.00 0.00 0.17064 14600.00 10200.00 0.00 0.17379 14800.00 10200.00 0.00 0.17472 14400.00 10400.00 0.00 0.17553 14600.00 10400.00 0.00 0.17863 14800.00 10400.00 0.00 0.17945 14400.00 10600.00 0.00 0.18034 14600.00 10600.00 0.00 0.18338 14800.00 10600.00 0.00 0.18408 14400.00 10800.00 0.00 0.18503 14600.00 10800.00 0.00 0.18800 14800.00 10800.00 0.00 0.18857 14400.00 11000.00 0.00 0.18988 14600.00 11000.00 0.00 0.19278 14800.00 11000.00 0.00 0.19321 14400.00 11200.00 0.00 0.19529 14600.00 11200.00 0.00 0.19810 14800.00 11200.00 0.00 0.19837 14400.00 11400.00 0.00 0.20111 14600.00 11400.00 0.00 0.20382 14800.00 11400.00 0.00 0.20391 14400.00 11600.00 0.00 0.20705 14600.00 11600.00 0.00 0.20964 14400.00 11800.00 0.00 0.21303 14600.00 11800.00 0.00 0.21549 14400.00 12000.00 0.00 0.21892 14600.00 12000.00 0.00 0.22123 14400.00 12200.00 0.00 0.22487 14600.00 12200.00 0.00 0.22701 14400.00 12400.00 0.00 0.23129 14600.00 12400.00 0.00 0.23322 14400.00 12600.00 0.00 0.23810 14600.00 12600.00 0.00 0.23978 14200.00 12800.00 0.00 0.23996 14400.00 12800.00 0.00 0.24485 14600.00 12800.00 0.00 0.24623 14200.00 13000.00 0.00 0.24671 14400.00 13000.00 0.00 0.25143 14600.00 13000.00 0.00 0.25244 14200.00 13200.00 0.00 0.25332 14400.00 13200.00 0.00 0.25779 14600.00 13200.00 0.00 0.25835 14200.00 13400.00 0.00 0.25990 14400.00 13400.00 0.00 0.26402 14600.00 13400.00 0.00 0.26403 14200.00 13600.00 0.00 0.26702 14400.00 13600.00 0.00 0.27063 14200.00 13800.00 0.00 0.27462 14400.00 13800.00 0.00 0.27743 14200.00 14000.00 0.00 0.28114 14400.00 14000.00 0.00 0.28287 14200.00 14200.00 0.00 0.28495 14400.00 14200.00 0.00 0.28542 14200.00 14400.00 10.00 0.28545 14200.00 14400.00 15.00 0.28556 14200.00 14400.00 20.00 0.28566 14200.00 14400.00 25.00 0.28576 14200.00 14400.00 30.00 0.28584 14200.00 14400.00 35.00 0.28590 14200.00 14400.00 40.00 0.28594 14200.00 14400.00 45.00 0.28597 14200.00 14400.00 50.00 0.28597 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14239.14 14334.67 50.00 0.28622 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/435.ctf NX, NY, NZ: 360 360 1 MODE : real