CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/437.mrc Output diagnostic file name Micrographs/437.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/437.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2474.000 8180.000 Mean, RMS : 4471.176 222.8016 TITLE 1: emi2mrc: 437.emi Mon Jan 20 12:10:02 2014 TITLE 2: kV 300, Mag 78000x, Def 16900, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X -445.853, Y 489.434, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.01560 8200.00 8000.00 0.00 0.01679 8400.00 8000.00 0.00 0.01801 8600.00 8000.00 0.00 0.01941 8800.00 8000.00 0.00 0.02107 9000.00 8000.00 0.00 0.02287 9200.00 8000.00 0.00 0.02466 9400.00 8000.00 0.00 0.02639 9600.00 8000.00 0.00 0.02806 9800.00 8000.00 0.00 0.02977 10000.00 8000.00 0.00 0.03165 10200.00 8000.00 0.00 0.03375 10400.00 8000.00 0.00 0.03604 10600.00 8000.00 0.00 0.03851 10800.00 8000.00 0.00 0.04120 11000.00 8000.00 0.00 0.04411 11200.00 8000.00 0.00 0.04730 11400.00 8000.00 0.00 0.05077 11600.00 8000.00 0.00 0.05447 11800.00 8000.00 0.00 0.05837 12000.00 8000.00 0.00 0.06259 12200.00 8000.00 0.00 0.06719 12400.00 8000.00 0.00 0.07223 12600.00 8000.00 0.00 0.07764 12800.00 8000.00 0.00 0.08316 13000.00 8000.00 0.00 0.08856 13200.00 8000.00 0.00 0.09381 13400.00 8000.00 0.00 0.09916 13600.00 8000.00 0.00 0.10498 13800.00 8000.00 0.00 0.11151 14000.00 8000.00 0.00 0.11851 14200.00 8000.00 0.00 0.12523 14400.00 8000.00 0.00 0.13081 14600.00 8000.00 0.00 0.13457 14800.00 8000.00 0.00 0.13639 15000.00 8000.00 0.00 0.13680 14600.00 8200.00 0.00 0.13708 14800.00 8200.00 0.00 0.13886 15000.00 8200.00 0.00 0.13918 14600.00 8400.00 0.00 0.13969 14800.00 8400.00 0.00 0.14141 15000.00 8400.00 0.00 0.14164 14600.00 8600.00 0.00 0.14273 14800.00 8600.00 0.00 0.14438 15000.00 8600.00 0.00 0.14452 14600.00 8800.00 0.00 0.14614 14800.00 8800.00 0.00 0.14771 15000.00 8800.00 0.00 0.14778 14600.00 9000.00 0.00 0.14964 14800.00 9000.00 0.00 0.15115 14600.00 9200.00 0.00 0.15311 14800.00 9200.00 0.00 0.15456 14600.00 9400.00 0.00 0.15645 14800.00 9400.00 0.00 0.15783 14600.00 9600.00 0.00 0.15969 14800.00 9600.00 0.00 0.16101 14600.00 9800.00 0.00 0.16324 14800.00 9800.00 0.00 0.16450 14600.00 10000.00 0.00 0.16730 14800.00 10000.00 0.00 0.16851 14600.00 10200.00 0.00 0.17167 14800.00 10200.00 0.00 0.17282 14600.00 10400.00 0.00 0.17622 14800.00 10400.00 0.00 0.17732 14400.00 10600.00 0.00 0.17742 14600.00 10600.00 0.00 0.18090 14800.00 10600.00 0.00 0.18192 14400.00 10800.00 0.00 0.18215 14600.00 10800.00 0.00 0.18559 14800.00 10800.00 0.00 0.18651 14400.00 11000.00 0.00 0.18711 14600.00 11000.00 0.00 0.19049 14800.00 11000.00 0.00 0.19129 14400.00 11200.00 0.00 0.19258 14600.00 11200.00 0.00 0.19589 14800.00 11200.00 0.00 0.19655 14400.00 11400.00 0.00 0.19839 14600.00 11400.00 0.00 0.20161 14800.00 11400.00 0.00 0.20210 14400.00 11600.00 0.00 0.20431 14600.00 11600.00 0.00 0.20743 14800.00 11600.00 0.00 0.20773 14400.00 11800.00 0.00 0.21035 14600.00 11800.00 0.00 0.21335 14800.00 11800.00 0.00 0.21345 14400.00 12000.00 0.00 0.21647 14600.00 12000.00 0.00 0.21935 14400.00 12200.00 0.00 0.22282 14600.00 12200.00 0.00 0.22556 14400.00 12400.00 0.00 0.22976 14600.00 12400.00 0.00 0.23233 14400.00 12600.00 0.00 0.23713 14600.00 12600.00 0.00 0.23950 14400.00 12800.00 0.00 0.24442 14600.00 12800.00 0.00 0.24655 14400.00 13000.00 0.00 0.25155 14600.00 13000.00 0.00 0.25337 14400.00 13200.00 0.00 0.25857 14600.00 13200.00 0.00 0.26000 14200.00 13400.00 0.00 0.26057 14400.00 13400.00 0.00 0.26565 14600.00 13400.00 0.00 0.26658 14200.00 13600.00 0.00 0.26871 14400.00 13600.00 0.00 0.27334 14600.00 13600.00 0.00 0.27357 14200.00 13800.00 0.00 0.27755 14400.00 13800.00 0.00 0.28140 14200.00 14000.00 0.00 0.28536 14400.00 14000.00 0.00 0.28809 14200.00 14200.00 0.00 0.29027 14400.00 14200.00 0.00 0.29164 14200.00 14400.00 20.00 0.29167 14200.00 14400.00 25.00 0.29179 14200.00 14400.00 30.00 0.29189 14200.00 14400.00 35.00 0.29198 14200.00 14400.00 40.00 0.29206 14200.00 14400.00 45.00 0.29211 14200.00 14400.00 50.00 0.29215 14200.00 14400.00 55.00 0.29216 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14264.07 14356.19 55.00 0.29239 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/437.ctf NX, NY, NZ: 360 360 1 MODE : real