CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/445.mrc Output diagnostic file name Micrographs/445.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/445.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2174.000 7968.000 Mean, RMS : 4467.942 221.5651 TITLE 1: emi2mrc: 445.emi Mon Jan 20 12:17:34 2014 TITLE 2: kV 300, Mag 78000x, Def 16900, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X -441.945, Y 490.138, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.03069 8200.00 8000.00 0.00 0.03213 8400.00 8000.00 0.00 0.03377 8600.00 8000.00 0.00 0.03585 8800.00 8000.00 0.00 0.03848 9000.00 8000.00 0.00 0.04149 9200.00 8000.00 0.00 0.04465 9400.00 8000.00 0.00 0.04792 9600.00 8000.00 0.00 0.05127 9800.00 8000.00 0.00 0.05483 10000.00 8000.00 0.00 0.05880 10200.00 8000.00 0.00 0.06321 10400.00 8000.00 0.00 0.06796 10600.00 8000.00 0.00 0.07303 10800.00 8000.00 0.00 0.07841 11000.00 8000.00 0.00 0.08410 11200.00 8000.00 0.00 0.09015 11400.00 8000.00 0.00 0.09648 11600.00 8000.00 0.00 0.10287 11800.00 8000.00 0.00 0.10921 12000.00 8000.00 0.00 0.11563 12200.00 8000.00 0.00 0.12237 12400.00 8000.00 0.00 0.12965 12600.00 8000.00 0.00 0.13739 12800.00 8000.00 0.00 0.14491 13000.00 8000.00 0.00 0.15126 13200.00 8000.00 0.00 0.15563 13400.00 8000.00 0.00 0.15771 13600.00 8000.00 0.00 0.15795 13200.00 8200.00 0.00 0.15910 13400.00 8200.00 0.00 0.16114 13600.00 8200.00 0.00 0.16131 13200.00 8400.00 0.00 0.16255 13400.00 8400.00 0.00 0.16455 13600.00 8400.00 0.00 0.16464 13200.00 8600.00 0.00 0.16638 13400.00 8600.00 0.00 0.16833 13600.00 8600.00 0.00 0.16834 13200.00 8800.00 0.00 0.17060 13400.00 8800.00 0.00 0.17250 13200.00 9000.00 0.00 0.17502 13400.00 9000.00 0.00 0.17687 13200.00 9200.00 0.00 0.17968 13400.00 9200.00 0.00 0.18147 13200.00 9400.00 0.00 0.18457 13400.00 9400.00 0.00 0.18629 13200.00 9600.00 0.00 0.18969 13400.00 9600.00 0.00 0.19132 13200.00 9800.00 0.00 0.19538 13400.00 9800.00 0.00 0.19691 13000.00 10000.00 0.00 0.19751 13200.00 10000.00 0.00 0.20174 13400.00 10000.00 0.00 0.20313 13000.00 10200.00 0.00 0.20421 13200.00 10200.00 0.00 0.20837 13400.00 10200.00 0.00 0.20959 13000.00 10400.00 0.00 0.21097 13200.00 10400.00 0.00 0.21505 13400.00 10400.00 0.00 0.21608 13000.00 10600.00 0.00 0.21773 13200.00 10600.00 0.00 0.22172 13400.00 10600.00 0.00 0.22257 13000.00 10800.00 0.00 0.22447 13200.00 10800.00 0.00 0.22836 13400.00 10800.00 0.00 0.22901 13000.00 11000.00 0.00 0.23157 13200.00 11000.00 0.00 0.23533 13400.00 11000.00 0.00 0.23573 13000.00 11200.00 0.00 0.23940 13200.00 11200.00 0.00 0.24299 13400.00 11200.00 0.00 0.24309 13000.00 11400.00 0.00 0.24762 13200.00 11400.00 0.00 0.25099 13000.00 11600.00 0.00 0.25578 13200.00 11600.00 0.00 0.25887 13000.00 11800.00 0.00 0.26382 13200.00 11800.00 0.00 0.26653 13000.00 12000.00 0.00 0.27154 13200.00 12000.00 0.00 0.27378 13000.00 12200.00 0.00 0.27908 13200.00 12200.00 0.00 0.28073 12800.00 12400.00 0.00 0.28161 13000.00 12400.00 0.00 0.28686 13200.00 12400.00 0.00 0.28770 12800.00 12600.00 0.00 0.28974 13000.00 12600.00 0.00 0.29410 12800.00 12800.00 0.00 0.29569 13000.00 12800.00 0.00 0.29886 13000.00 13000.00 0.00 0.29995 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13032.96 12914.20 -36.88 0.30031 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/445.ctf NX, NY, NZ: 360 360 1 MODE : real