CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/446.mrc Output diagnostic file name Micrographs/446.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/446.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2578.000 8058.000 Mean, RMS : 4472.424 219.1123 TITLE 1: emi2mrc: 446.emi Mon Jan 20 12:18:12 2014 TITLE 2: kV 300, Mag 78000x, Def 16900, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X -440.099, Y 492.378, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.01765 8200.00 8000.00 0.00 0.01905 8400.00 8000.00 0.00 0.02040 8600.00 8000.00 0.00 0.02191 8800.00 8000.00 0.00 0.02383 9000.00 8000.00 0.00 0.02619 9200.00 8000.00 0.00 0.02885 9400.00 8000.00 0.00 0.03162 9600.00 8000.00 0.00 0.03429 9800.00 8000.00 0.00 0.03694 10000.00 8000.00 0.00 0.03990 10200.00 8000.00 0.00 0.04348 10400.00 8000.00 0.00 0.04772 10600.00 8000.00 0.00 0.05245 10800.00 8000.00 0.00 0.05731 11000.00 8000.00 0.00 0.06203 11200.00 8000.00 0.00 0.06671 11400.00 8000.00 0.00 0.07167 11600.00 8000.00 0.00 0.07710 11800.00 8000.00 0.00 0.08301 12000.00 8000.00 0.00 0.08915 12200.00 8000.00 0.00 0.09518 12400.00 8000.00 0.00 0.10108 12600.00 8000.00 0.00 0.10718 12800.00 8000.00 0.00 0.11387 13000.00 8000.00 0.00 0.12123 13200.00 8000.00 0.00 0.12892 13400.00 8000.00 0.00 0.13605 13600.00 8000.00 0.00 0.14173 13800.00 8000.00 0.00 0.14552 14000.00 8000.00 0.00 0.14744 14200.00 8000.00 0.00 0.14793 13800.00 8200.00 0.00 0.14861 14000.00 8200.00 0.00 0.15046 14200.00 8200.00 0.00 0.15086 13800.00 8400.00 0.00 0.15172 14000.00 8400.00 0.00 0.15350 14200.00 8400.00 0.00 0.15380 13800.00 8600.00 0.00 0.15523 14000.00 8600.00 0.00 0.15692 14200.00 8600.00 0.00 0.15712 13800.00 8800.00 0.00 0.15913 14000.00 8800.00 0.00 0.16073 14200.00 8800.00 0.00 0.16083 13800.00 9000.00 0.00 0.16319 14000.00 9000.00 0.00 0.16472 13800.00 9200.00 0.00 0.16734 14000.00 9200.00 0.00 0.16879 13800.00 9400.00 0.00 0.17147 14000.00 9400.00 0.00 0.17285 13800.00 9600.00 0.00 0.17559 14000.00 9600.00 0.00 0.17688 13800.00 9800.00 0.00 0.18009 14000.00 9800.00 0.00 0.18127 13600.00 10000.00 0.00 0.18187 13800.00 10000.00 0.00 0.18516 14000.00 10000.00 0.00 0.18618 13600.00 10200.00 0.00 0.18734 13800.00 10200.00 0.00 0.19049 14000.00 10200.00 0.00 0.19131 13600.00 10400.00 0.00 0.19286 13800.00 10400.00 0.00 0.19584 14000.00 10400.00 0.00 0.19645 13600.00 10600.00 0.00 0.19829 13800.00 10600.00 0.00 0.20110 14000.00 10600.00 0.00 0.20150 13600.00 10800.00 0.00 0.20355 13800.00 10800.00 0.00 0.20618 14000.00 10800.00 0.00 0.20636 13600.00 11000.00 0.00 0.20899 13800.00 11000.00 0.00 0.21144 13600.00 11200.00 0.00 0.21510 13800.00 11200.00 0.00 0.21733 13600.00 11400.00 0.00 0.22167 13800.00 11400.00 0.00 0.22365 13600.00 11600.00 0.00 0.22829 13800.00 11600.00 0.00 0.22998 13400.00 11800.00 0.00 0.23008 13600.00 11800.00 0.00 0.23469 13800.00 11800.00 0.00 0.23607 13400.00 12000.00 0.00 0.23627 13600.00 12000.00 0.00 0.24065 13800.00 12000.00 0.00 0.24167 13400.00 12200.00 0.00 0.24233 13600.00 12200.00 0.00 0.24643 13800.00 12200.00 0.00 0.24704 13400.00 12400.00 0.00 0.24915 13600.00 12400.00 0.00 0.25284 13800.00 12400.00 0.00 0.25289 13400.00 12600.00 0.00 0.25688 13600.00 12600.00 0.00 0.25993 13400.00 12800.00 0.00 0.26473 13600.00 12800.00 0.00 0.26688 13400.00 13000.00 0.00 0.27178 13600.00 13000.00 0.00 0.27277 13400.00 13200.00 0.00 0.27674 13400.00 13400.00 0.00 0.27828 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13455.82 13357.61 -29.41 0.27844 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/446.ctf NX, NY, NZ: 360 360 1 MODE : real