CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/447.mrc Output diagnostic file name Micrographs/447.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/447.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2404.000 7682.000 Mean, RMS : 4411.692 218.4952 TITLE 1: emi2mrc: 447.emi Mon Jan 20 12:19:03 2014 TITLE 2: kV 300, Mag 78000x, Def 16900, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X -438.719, Y 494.606, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.02476 8200.00 8000.00 0.00 0.02661 8400.00 8000.00 0.00 0.02815 8600.00 8000.00 0.00 0.02946 8800.00 8000.00 0.00 0.03077 9000.00 8000.00 0.00 0.03225 9200.00 8000.00 0.00 0.03401 9400.00 8000.00 0.00 0.03607 9600.00 8000.00 0.00 0.03833 9800.00 8000.00 0.00 0.04072 10000.00 8000.00 0.00 0.04335 10200.00 8000.00 0.00 0.04631 10400.00 8000.00 0.00 0.04961 10600.00 8000.00 0.00 0.05321 10800.00 8000.00 0.00 0.05693 11000.00 8000.00 0.00 0.06062 11200.00 8000.00 0.00 0.06437 11400.00 8000.00 0.00 0.06843 11600.00 8000.00 0.00 0.07300 11800.00 8000.00 0.00 0.07815 12000.00 8000.00 0.00 0.08371 12200.00 8000.00 0.00 0.08931 12400.00 8000.00 0.00 0.09470 12600.00 8000.00 0.00 0.09995 12800.00 8000.00 0.00 0.10530 13000.00 8000.00 0.00 0.11106 13200.00 8000.00 0.00 0.11738 13400.00 8000.00 0.00 0.12399 13600.00 8000.00 0.00 0.13029 13800.00 8000.00 0.00 0.13568 14000.00 8000.00 0.00 0.13962 14200.00 8000.00 0.00 0.14193 14400.00 8000.00 0.00 0.14286 14600.00 8000.00 0.00 0.14287 14200.00 8200.00 0.00 0.14513 14400.00 8200.00 0.00 0.14602 14000.00 8400.00 0.00 0.14614 14200.00 8400.00 0.00 0.14841 14400.00 8400.00 0.00 0.14924 14000.00 8600.00 0.00 0.14978 14200.00 8600.00 0.00 0.15198 14400.00 8600.00 0.00 0.15271 14000.00 8800.00 0.00 0.15360 14200.00 8800.00 0.00 0.15572 14400.00 8800.00 0.00 0.15633 14000.00 9000.00 0.00 0.15733 14200.00 9000.00 0.00 0.15935 14400.00 9000.00 0.00 0.15984 14000.00 9200.00 0.00 0.16090 14200.00 9200.00 0.00 0.16284 14400.00 9200.00 0.00 0.16320 14000.00 9400.00 0.00 0.16428 14200.00 9400.00 0.00 0.16613 14400.00 9400.00 0.00 0.16640 14000.00 9600.00 0.00 0.16759 14200.00 9600.00 0.00 0.16937 14400.00 9600.00 0.00 0.16955 14000.00 9800.00 0.00 0.17130 14200.00 9800.00 0.00 0.17302 14400.00 9800.00 0.00 0.17313 14000.00 10000.00 0.00 0.17571 14200.00 10000.00 0.00 0.17737 14400.00 10000.00 0.00 0.17739 14000.00 10200.00 0.00 0.18058 14200.00 10200.00 0.00 0.18218 14000.00 10400.00 0.00 0.18572 14200.00 10400.00 0.00 0.18726 13800.00 10600.00 0.00 0.18738 14000.00 10600.00 0.00 0.19097 14200.00 10600.00 0.00 0.19245 13800.00 10800.00 0.00 0.19263 14000.00 10800.00 0.00 0.19620 14200.00 10800.00 0.00 0.19763 13800.00 11000.00 0.00 0.19820 14000.00 11000.00 0.00 0.20175 14200.00 11000.00 0.00 0.20308 13800.00 11200.00 0.00 0.20458 14000.00 11200.00 0.00 0.20806 14200.00 11200.00 0.00 0.20927 13800.00 11400.00 0.00 0.21166 14000.00 11400.00 0.00 0.21504 14200.00 11400.00 0.00 0.21607 13800.00 11600.00 0.00 0.21905 14000.00 11600.00 0.00 0.22228 14200.00 11600.00 0.00 0.22308 13800.00 11800.00 0.00 0.22640 14000.00 11800.00 0.00 0.22944 14200.00 11800.00 0.00 0.22997 13800.00 12000.00 0.00 0.23326 14000.00 12000.00 0.00 0.23609 14200.00 12000.00 0.00 0.23633 13800.00 12200.00 0.00 0.23970 14000.00 12200.00 0.00 0.24229 13800.00 12400.00 0.00 0.24645 14000.00 12400.00 0.00 0.24874 13600.00 12600.00 0.00 0.24921 13800.00 12600.00 0.00 0.25387 14000.00 12600.00 0.00 0.25575 13600.00 12800.00 0.00 0.25746 13800.00 12800.00 0.00 0.26167 14000.00 12800.00 0.00 0.26300 13600.00 13000.00 0.00 0.26585 13800.00 13000.00 0.00 0.26939 14000.00 13000.00 0.00 0.27002 13600.00 13200.00 0.00 0.27327 13800.00 13200.00 0.00 0.27597 13600.00 13400.00 0.00 0.27829 13800.00 13400.00 0.00 0.28012 13600.00 13600.00 0.00 0.28067 13800.00 13600.00 0.00 0.28171 13600.00 13800.00 40.00 0.28177 13600.00 13800.00 45.00 0.28185 13600.00 13800.00 50.00 0.28192 13600.00 13800.00 55.00 0.28196 13600.00 13800.00 60.00 0.28199 13600.00 13800.00 65.00 0.28199 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13624.14 13791.45 62.90 0.28201 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/447.ctf NX, NY, NZ: 360 360 1 MODE : real