CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/452.mrc Output diagnostic file name Micrographs/452.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/452.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2506.000 8722.000 Mean, RMS : 4471.680 223.2561 TITLE 1: emi2mrc: 452.emi Mon Jan 20 12:24:38 2014 TITLE 2: kV 300, Mag 78000x, Def 16900, Spot 4, Int 55.08%, Obj 88.07%, Dif 73.67% TITLE 3: X -428.694, Y 506.206, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01104 8200.00 8000.00 0.00 -0.01082 8400.00 8000.00 0.00 -0.01056 8600.00 8000.00 0.00 -0.01036 8800.00 8000.00 0.00 -0.01030 9600.00 8000.00 0.00 -0.01015 9800.00 8000.00 0.00 -0.00969 10000.00 8000.00 0.00 -0.00907 10200.00 8000.00 0.00 -0.00834 10400.00 8000.00 0.00 -0.00754 10600.00 8000.00 0.00 -0.00672 10800.00 8000.00 0.00 -0.00587 11000.00 8000.00 0.00 -0.00499 11200.00 8000.00 0.00 -0.00408 11400.00 8000.00 0.00 -0.00312 11600.00 8000.00 0.00 -0.00208 11800.00 8000.00 0.00 -0.00095 12000.00 8000.00 0.00 0.00029 12200.00 8000.00 0.00 0.00166 12400.00 8000.00 0.00 0.00317 12600.00 8000.00 0.00 0.00480 12800.00 8000.00 0.00 0.00652 13000.00 8000.00 0.00 0.00827 13200.00 8000.00 0.00 0.01005 13400.00 8000.00 0.00 0.01185 13600.00 8000.00 0.00 0.01374 13800.00 8000.00 0.00 0.01578 14000.00 8000.00 0.00 0.01801 14200.00 8000.00 0.00 0.02041 14400.00 8000.00 0.00 0.02292 14600.00 8000.00 0.00 0.02547 14800.00 8000.00 0.00 0.02800 15000.00 8000.00 0.00 0.03052 15200.00 8000.00 0.00 0.03305 15400.00 8000.00 0.00 0.03558 15600.00 8000.00 0.00 0.03807 15800.00 8000.00 0.00 0.04050 16000.00 8000.00 0.00 0.04289 16200.00 8000.00 0.00 0.04533 16400.00 8000.00 0.00 0.04794 16600.00 8000.00 0.00 0.05073 16800.00 8000.00 0.00 0.05363 17000.00 8000.00 0.00 0.05652 17200.00 8000.00 0.00 0.05928 17400.00 8000.00 0.00 0.06194 17600.00 8000.00 0.00 0.06469 17800.00 8000.00 0.00 0.06770 18000.00 8000.00 0.00 0.07103 18200.00 8000.00 0.00 0.07454 18400.00 8000.00 0.00 0.07793 18600.00 8000.00 0.00 0.08087 18800.00 8000.00 0.00 0.08321 19000.00 8000.00 0.00 0.08493 19200.00 8000.00 0.00 0.08614 19400.00 8000.00 0.00 0.08699 19600.00 8000.00 0.00 0.08755 19800.00 8000.00 0.00 0.08785 20000.00 8000.00 0.00 0.08792 19600.00 8200.00 0.00 0.08839 19800.00 8200.00 0.00 0.08866 20000.00 8200.00 0.00 0.08871 19600.00 8400.00 0.00 0.08918 19800.00 8400.00 0.00 0.08944 20000.00 8400.00 0.00 0.08948 19400.00 8600.00 0.00 0.08963 19600.00 8600.00 0.00 0.09012 19800.00 8600.00 0.00 0.09038 20000.00 8600.00 0.00 0.09045 19400.00 8800.00 0.00 0.09075 19600.00 8800.00 0.00 0.09126 19800.00 8800.00 0.00 0.09154 20000.00 8800.00 0.00 0.09163 19400.00 9000.00 0.00 0.09202 19600.00 9000.00 0.00 0.09257 19800.00 9000.00 0.00 0.09287 20000.00 9000.00 0.00 0.09299 19400.00 9200.00 0.00 0.09346 19600.00 9200.00 0.00 0.09403 19800.00 9200.00 0.00 0.09435 20000.00 9200.00 0.00 0.09448 20200.00 9200.00 0.00 0.09450 19400.00 9400.00 0.00 0.09502 19600.00 9400.00 0.00 0.09560 19800.00 9400.00 0.00 0.09592 20000.00 9400.00 0.00 0.09606 20200.00 9400.00 0.00 0.09608 19400.00 9600.00 0.00 0.09665 19600.00 9600.00 0.00 0.09722 19800.00 9600.00 0.00 0.09754 20000.00 9600.00 0.00 0.09767 20200.00 9600.00 0.00 0.09768 19400.00 9800.00 0.00 0.09842 19600.00 9800.00 0.00 0.09898 19800.00 9800.00 0.00 0.09928 20000.00 9800.00 0.00 0.09939 19200.00 10000.00 0.00 0.09953 19400.00 10000.00 0.00 0.10034 19600.00 10000.00 0.00 0.10088 19800.00 10000.00 0.00 0.10117 20000.00 10000.00 0.00 0.10125 19200.00 10200.00 0.00 0.10152 19400.00 10200.00 0.00 0.10231 19600.00 10200.00 0.00 0.10284 19800.00 10200.00 0.00 0.10311 20000.00 10200.00 0.00 0.10317 19200.00 10400.00 0.00 0.10351 19400.00 10400.00 0.00 0.10430 19600.00 10400.00 0.00 0.10481 19800.00 10400.00 0.00 0.10507 20000.00 10400.00 0.00 0.10513 19200.00 10600.00 0.00 0.10551 19400.00 10600.00 0.00 0.10630 19600.00 10600.00 0.00 0.10681 19800.00 10600.00 0.00 0.10707 20000.00 10600.00 0.00 0.10713 19200.00 10800.00 0.00 0.10753 19400.00 10800.00 0.00 0.10832 19600.00 10800.00 0.00 0.10884 19800.00 10800.00 0.00 0.10909 20000.00 10800.00 0.00 0.10916 19200.00 11000.00 0.00 0.10968 19400.00 11000.00 0.00 0.11048 19600.00 11000.00 0.00 0.11100 19800.00 11000.00 0.00 0.11125 20000.00 11000.00 0.00 0.11131 19200.00 11200.00 0.00 0.11207 19400.00 11200.00 0.00 0.11287 19600.00 11200.00 0.00 0.11337 19800.00 11200.00 0.00 0.11360 20000.00 11200.00 0.00 0.11362 19200.00 11400.00 0.00 0.11461 19400.00 11400.00 0.00 0.11538 19600.00 11400.00 0.00 0.11586 19800.00 11400.00 0.00 0.11604 19200.00 11600.00 0.00 0.11718 19400.00 11600.00 0.00 0.11792 19600.00 11600.00 0.00 0.11836 19800.00 11600.00 0.00 0.11848 19000.00 11800.00 0.00 0.11863 19200.00 11800.00 0.00 0.11976 19400.00 11800.00 0.00 0.12046 19600.00 11800.00 0.00 0.12084 19800.00 11800.00 0.00 0.12091 19000.00 12000.00 0.00 0.12120 19200.00 12000.00 0.00 0.12229 19400.00 12000.00 0.00 0.12295 19600.00 12000.00 0.00 0.12327 19800.00 12000.00 0.00 0.12329 19000.00 12200.00 0.00 0.12379 19200.00 12200.00 0.00 0.12484 19400.00 12200.00 0.00 0.12547 19600.00 12200.00 0.00 0.12574 19000.00 12400.00 0.00 0.12658 19200.00 12400.00 0.00 0.12761 19400.00 12400.00 0.00 0.12819 19600.00 12400.00 0.00 0.12842 19000.00 12600.00 0.00 0.12955 19200.00 12600.00 0.00 0.13056 19400.00 12600.00 0.00 0.13110 19600.00 12600.00 0.00 0.13127 19000.00 12800.00 0.00 0.13255 19200.00 12800.00 0.00 0.13353 19400.00 12800.00 0.00 0.13402 19600.00 12800.00 0.00 0.13416 19000.00 13000.00 0.00 0.13554 19200.00 13000.00 0.00 0.13649 19400.00 13000.00 0.00 0.13695 19600.00 13000.00 0.00 0.13705 19000.00 13200.00 0.00 0.13850 19200.00 13200.00 0.00 0.13942 19400.00 13200.00 0.00 0.13985 19600.00 13200.00 0.00 0.13991 19000.00 13400.00 0.00 0.14146 19200.00 13400.00 0.00 0.14235 19400.00 13400.00 0.00 0.14274 19600.00 13400.00 0.00 0.14276 18800.00 13600.00 0.00 0.14304 19000.00 13600.00 0.00 0.14461 19200.00 13600.00 0.00 0.14547 19400.00 13600.00 0.00 0.14580 18800.00 13800.00 0.00 0.14650 19000.00 13800.00 0.00 0.14803 19200.00 13800.00 0.00 0.14882 19400.00 13800.00 0.00 0.14909 18800.00 14000.00 0.00 0.15000 19000.00 14000.00 0.00 0.15148 19200.00 14000.00 0.00 0.15220 19400.00 14000.00 0.00 0.15239 18800.00 14200.00 0.00 0.15342 19000.00 14200.00 0.00 0.15484 19200.00 14200.00 0.00 0.15550 19400.00 14200.00 0.00 0.15560 18800.00 14400.00 0.00 0.15673 19000.00 14400.00 0.00 0.15810 19200.00 14400.00 0.00 0.15869 19400.00 14400.00 0.00 0.15873 18800.00 14600.00 0.00 0.15994 19000.00 14600.00 0.00 0.16127 19200.00 14600.00 0.00 0.16180 18800.00 14800.00 0.00 0.16330 19000.00 14800.00 0.00 0.16458 19200.00 14800.00 0.00 0.16505 18800.00 15000.00 0.00 0.16710 19000.00 15000.00 0.00 0.16833 19200.00 15000.00 0.00 0.16872 18600.00 15200.00 0.00 0.16901 18800.00 15200.00 0.00 0.17120 19000.00 15200.00 0.00 0.17236 19200.00 15200.00 0.00 0.17265 18600.00 15400.00 0.00 0.17320 18800.00 15400.00 0.00 0.17532 19000.00 15400.00 0.00 0.17638 19200.00 15400.00 0.00 0.17656 18600.00 15600.00 0.00 0.17726 18800.00 15600.00 0.00 0.17930 19000.00 15600.00 0.00 0.18026 19200.00 15600.00 0.00 0.18032 18600.00 15800.00 0.00 0.18109 18800.00 15800.00 0.00 0.18305 19000.00 15800.00 0.00 0.18389 18600.00 16000.00 0.00 0.18483 18800.00 16000.00 0.00 0.18670 19000.00 16000.00 0.00 0.18744 18600.00 16200.00 0.00 0.18891 18800.00 16200.00 0.00 0.19069 19000.00 16200.00 0.00 0.19130 18600.00 16400.00 0.00 0.19345 18800.00 16400.00 0.00 0.19512 19000.00 16400.00 0.00 0.19558 18600.00 16600.00 0.00 0.19817 18800.00 16600.00 0.00 0.19971 19000.00 16600.00 0.00 0.20000 18600.00 16800.00 0.00 0.20285 18800.00 16800.00 0.00 0.20424 19000.00 16800.00 0.00 0.20435 18400.00 17000.00 0.00 0.20444 18600.00 17000.00 0.00 0.20734 18800.00 17000.00 0.00 0.20858 18400.00 17200.00 0.00 0.20892 18600.00 17200.00 0.00 0.21174 18800.00 17200.00 0.00 0.21281 18400.00 17400.00 0.00 0.21398 18600.00 17400.00 0.00 0.21666 18800.00 17400.00 0.00 0.21747 18400.00 17600.00 0.00 0.22010 18600.00 17600.00 0.00 0.22249 18800.00 17600.00 0.00 0.22288 18400.00 17800.00 0.00 0.22684 18600.00 17800.00 0.00 0.22872 18200.00 18000.00 0.00 0.22954 18400.00 18000.00 0.00 0.23323 18600.00 18000.00 0.00 0.23436 18200.00 18200.00 0.00 0.23520 18400.00 18200.00 0.00 0.23806 18600.00 18200.00 0.00 0.23823 18400.00 18400.00 0.00 0.23986 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18480.74 18356.57 -35.72 0.24008 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/452.ctf NX, NY, NZ: 360 360 1 MODE : real