CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/463.mrc Output diagnostic file name Micrographs/463.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/463.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2310.000 8068.000 Mean, RMS : 4443.940 212.7473 TITLE 1: emi2mrc: 463.emi Mon Jan 20 12:35:13 2014 TITLE 2: kV 300, Mag 78000x, Def 22640, Spot 4, Int 55.08%, Obj 88.09%, Dif 73.67% TITLE 3: X -445.191, Y 482.115, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 0.30989 8200.00 8000.00 0.00 0.31163 8000.00 8200.00 0.00 0.31179 8000.00 8200.00 5.00 0.31184 8000.00 8200.00 10.00 0.31189 8000.00 8200.00 15.00 0.31194 8000.00 8200.00 20.00 0.31199 8000.00 8200.00 25.00 0.31203 8000.00 8200.00 30.00 0.31206 8000.00 8200.00 35.00 0.31209 8000.00 8200.00 40.00 0.31210 8000.00 8200.00 45.00 0.31211 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8095.37 8118.47 38.81 0.31329 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/463.ctf NX, NY, NZ: 360 360 1 MODE : real