CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/469.mrc Output diagnostic file name Micrographs/469.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/469.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2058.000 8224.000 Mean, RMS : 4429.236 220.0919 TITLE 1: emi2mrc: 469.emi Mon Jan 20 12:40:00 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.05%, Dif 73.67% TITLE 3: X -439.597, Y 485.229, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01774 8200.00 8000.00 0.00 -0.01760 8400.00 8000.00 0.00 -0.01738 8600.00 8000.00 0.00 -0.01721 8800.00 8000.00 0.00 -0.01715 9000.00 8000.00 0.00 -0.01714 9200.00 8000.00 0.00 -0.01712 9400.00 8000.00 0.00 -0.01705 9600.00 8000.00 0.00 -0.01690 9800.00 8000.00 0.00 -0.01670 10000.00 8000.00 0.00 -0.01654 10200.00 8000.00 0.00 -0.01641 10400.00 8000.00 0.00 -0.01627 10600.00 8000.00 0.00 -0.01606 10800.00 8000.00 0.00 -0.01571 11000.00 8000.00 0.00 -0.01517 11200.00 8000.00 0.00 -0.01450 11400.00 8000.00 0.00 -0.01374 11600.00 8000.00 0.00 -0.01292 11800.00 8000.00 0.00 -0.01203 12000.00 8000.00 0.00 -0.01108 12200.00 8000.00 0.00 -0.01002 12400.00 8000.00 0.00 -0.00887 12600.00 8000.00 0.00 -0.00766 12800.00 8000.00 0.00 -0.00641 13000.00 8000.00 0.00 -0.00514 13200.00 8000.00 0.00 -0.00382 13400.00 8000.00 0.00 -0.00242 13600.00 8000.00 0.00 -0.00088 13800.00 8000.00 0.00 0.00079 14000.00 8000.00 0.00 0.00256 14200.00 8000.00 0.00 0.00441 14400.00 8000.00 0.00 0.00630 14600.00 8000.00 0.00 0.00822 14800.00 8000.00 0.00 0.01019 15000.00 8000.00 0.00 0.01226 15200.00 8000.00 0.00 0.01441 15400.00 8000.00 0.00 0.01658 15600.00 8000.00 0.00 0.01871 15800.00 8000.00 0.00 0.02078 16000.00 8000.00 0.00 0.02282 16200.00 8000.00 0.00 0.02495 16400.00 8000.00 0.00 0.02727 16600.00 8000.00 0.00 0.02980 16800.00 8000.00 0.00 0.03244 17000.00 8000.00 0.00 0.03502 17200.00 8000.00 0.00 0.03742 17400.00 8000.00 0.00 0.03965 17600.00 8000.00 0.00 0.04185 17800.00 8000.00 0.00 0.04415 18000.00 8000.00 0.00 0.04664 18200.00 8000.00 0.00 0.04930 18400.00 8000.00 0.00 0.05202 18600.00 8000.00 0.00 0.05472 18800.00 8000.00 0.00 0.05744 19000.00 8000.00 0.00 0.06029 19200.00 8000.00 0.00 0.06337 19400.00 8000.00 0.00 0.06665 19600.00 8000.00 0.00 0.06998 19800.00 8000.00 0.00 0.07307 20000.00 8000.00 0.00 0.07571 20200.00 8000.00 0.00 0.07776 20400.00 8000.00 0.00 0.07918 20600.00 8000.00 0.00 0.08006 20800.00 8000.00 0.00 0.08051 21000.00 8000.00 0.00 0.08062 20600.00 8200.00 0.00 0.08075 20800.00 8200.00 0.00 0.08121 21000.00 8200.00 0.00 0.08133 20600.00 8400.00 0.00 0.08154 20800.00 8400.00 0.00 0.08201 21000.00 8400.00 0.00 0.08214 20600.00 8600.00 0.00 0.08263 20800.00 8600.00 0.00 0.08310 21000.00 8600.00 0.00 0.08322 20600.00 8800.00 0.00 0.08397 20800.00 8800.00 0.00 0.08443 21000.00 8800.00 0.00 0.08453 20600.00 9000.00 0.00 0.08540 20800.00 9000.00 0.00 0.08584 21000.00 9000.00 0.00 0.08591 20400.00 9200.00 0.00 0.08593 20600.00 9200.00 0.00 0.08680 20800.00 9200.00 0.00 0.08720 21000.00 9200.00 0.00 0.08725 20400.00 9400.00 0.00 0.08726 20600.00 9400.00 0.00 0.08810 20800.00 9400.00 0.00 0.08847 21000.00 9400.00 0.00 0.08847 20400.00 9600.00 0.00 0.08852 20600.00 9600.00 0.00 0.08932 20800.00 9600.00 0.00 0.08965 20400.00 9800.00 0.00 0.08990 20600.00 9800.00 0.00 0.09067 20800.00 9800.00 0.00 0.09096 20400.00 10000.00 0.00 0.09152 20600.00 10000.00 0.00 0.09225 20800.00 10000.00 0.00 0.09251 20400.00 10200.00 0.00 0.09326 20600.00 10200.00 0.00 0.09396 20800.00 10200.00 0.00 0.09419 20400.00 10400.00 0.00 0.09502 20600.00 10400.00 0.00 0.09568 20800.00 10400.00 0.00 0.09587 20400.00 10600.00 0.00 0.09670 20600.00 10600.00 0.00 0.09732 20800.00 10600.00 0.00 0.09747 20400.00 10800.00 0.00 0.09826 20600.00 10800.00 0.00 0.09883 20800.00 10800.00 0.00 0.09895 20400.00 11000.00 0.00 0.09983 20600.00 11000.00 0.00 0.10037 20800.00 11000.00 0.00 0.10045 20400.00 11200.00 0.00 0.10161 20600.00 11200.00 0.00 0.10212 20800.00 11200.00 0.00 0.10219 20200.00 11400.00 0.00 0.10245 20400.00 11400.00 0.00 0.10358 20600.00 11400.00 0.00 0.10408 20800.00 11400.00 0.00 0.10413 20200.00 11600.00 0.00 0.10453 20400.00 11600.00 0.00 0.10565 20600.00 11600.00 0.00 0.10614 20800.00 11600.00 0.00 0.10618 20200.00 11800.00 0.00 0.10661 20400.00 11800.00 0.00 0.10772 20600.00 11800.00 0.00 0.10818 20800.00 11800.00 0.00 0.10820 20200.00 12000.00 0.00 0.10858 20400.00 12000.00 0.00 0.10968 20600.00 12000.00 0.00 0.11012 20200.00 12200.00 0.00 0.11052 20400.00 12200.00 0.00 0.11160 20600.00 12200.00 0.00 0.11202 20200.00 12400.00 0.00 0.11263 20400.00 12400.00 0.00 0.11368 20600.00 12400.00 0.00 0.11408 20200.00 12600.00 0.00 0.11496 20400.00 12600.00 0.00 0.11599 20600.00 12600.00 0.00 0.11635 20200.00 12800.00 0.00 0.11741 20400.00 12800.00 0.00 0.11840 20600.00 12800.00 0.00 0.11873 20200.00 13000.00 0.00 0.11989 20400.00 13000.00 0.00 0.12084 20600.00 13000.00 0.00 0.12111 20200.00 13200.00 0.00 0.12224 20400.00 13200.00 0.00 0.12313 20600.00 13200.00 0.00 0.12336 20200.00 13400.00 0.00 0.12441 20400.00 13400.00 0.00 0.12526 20600.00 13400.00 0.00 0.12545 20200.00 13600.00 0.00 0.12661 20400.00 13600.00 0.00 0.12742 20600.00 13600.00 0.00 0.12758 20200.00 13800.00 0.00 0.12901 20400.00 13800.00 0.00 0.12980 20600.00 13800.00 0.00 0.12992 20000.00 14000.00 0.00 0.12998 20200.00 14000.00 0.00 0.13159 20400.00 14000.00 0.00 0.13236 20600.00 14000.00 0.00 0.13244 20000.00 14200.00 0.00 0.13271 20200.00 14200.00 0.00 0.13431 20400.00 14200.00 0.00 0.13504 20600.00 14200.00 0.00 0.13508 20000.00 14400.00 0.00 0.13550 20200.00 14400.00 0.00 0.13708 20400.00 14400.00 0.00 0.13777 20000.00 14600.00 0.00 0.13822 20200.00 14600.00 0.00 0.13976 20400.00 14600.00 0.00 0.14042 20000.00 14800.00 0.00 0.14094 20200.00 14800.00 0.00 0.14245 20400.00 14800.00 0.00 0.14306 20000.00 15000.00 0.00 0.14382 20200.00 15000.00 0.00 0.14529 20400.00 15000.00 0.00 0.14585 20000.00 15200.00 0.00 0.14681 20200.00 15200.00 0.00 0.14824 20400.00 15200.00 0.00 0.14873 20000.00 15400.00 0.00 0.14982 20200.00 15400.00 0.00 0.15119 20400.00 15400.00 0.00 0.15162 20000.00 15600.00 0.00 0.15284 20200.00 15600.00 0.00 0.15417 20400.00 15600.00 0.00 0.15453 20000.00 15800.00 0.00 0.15579 20200.00 15800.00 0.00 0.15707 20400.00 15800.00 0.00 0.15738 20000.00 16000.00 0.00 0.15868 20200.00 16000.00 0.00 0.15992 20400.00 16000.00 0.00 0.16018 20000.00 16200.00 0.00 0.16177 20200.00 16200.00 0.00 0.16296 20400.00 16200.00 0.00 0.16315 20000.00 16400.00 0.00 0.16507 20200.00 16400.00 0.00 0.16621 20400.00 16400.00 0.00 0.16634 20000.00 16600.00 0.00 0.16842 20200.00 16600.00 0.00 0.16950 20400.00 16600.00 0.00 0.16957 19800.00 16800.00 0.00 0.16958 20000.00 16800.00 0.00 0.17175 20200.00 16800.00 0.00 0.17279 20400.00 16800.00 0.00 0.17279 19800.00 17000.00 0.00 0.17288 20000.00 17000.00 0.00 0.17503 20200.00 17000.00 0.00 0.17603 19800.00 17200.00 0.00 0.17612 20000.00 17200.00 0.00 0.17825 20200.00 17200.00 0.00 0.17920 19800.00 17400.00 0.00 0.17956 20000.00 17400.00 0.00 0.18166 20200.00 17400.00 0.00 0.18254 19800.00 17600.00 0.00 0.18336 20000.00 17600.00 0.00 0.18540 20200.00 17600.00 0.00 0.18618 19800.00 17800.00 0.00 0.18731 20000.00 17800.00 0.00 0.18926 20200.00 17800.00 0.00 0.18990 19800.00 18000.00 0.00 0.19116 20000.00 18000.00 0.00 0.19300 20200.00 18000.00 0.00 0.19347 19800.00 18200.00 0.00 0.19480 20000.00 18200.00 0.00 0.19650 20200.00 18200.00 0.00 0.19680 19800.00 18400.00 0.00 0.19811 20000.00 18400.00 0.00 0.19968 20200.00 18400.00 0.00 0.19980 19800.00 18600.00 0.00 0.20141 20000.00 18600.00 0.00 0.20282 19800.00 18800.00 0.00 0.20515 20000.00 18800.00 0.00 0.20632 19600.00 19000.00 0.00 0.20645 19800.00 19000.00 0.00 0.20921 20000.00 19000.00 0.00 0.21002 19600.00 19200.00 0.00 0.21068 19800.00 19200.00 0.00 0.21307 20000.00 19200.00 0.00 0.21340 19600.00 19400.00 0.00 0.21438 19800.00 19400.00 0.00 0.21622 19600.00 19600.00 0.00 0.21677 19800.00 19600.00 0.00 0.21792 19600.00 19800.00 40.00 0.21793 19600.00 19800.00 45.00 0.21800 19600.00 19800.00 50.00 0.21806 19600.00 19800.00 55.00 0.21810 19600.00 19800.00 60.00 0.21813 19600.00 19800.00 65.00 0.21814 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19650.00 19834.59 60.77 0.21826 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/469.ctf NX, NY, NZ: 360 360 1 MODE : real