CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/471.mrc Output diagnostic file name Micrographs/471.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/471.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2278.000 7938.000 Mean, RMS : 4342.748 214.0524 TITLE 1: emi2mrc: 471.emi Mon Jan 20 12:41:16 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.05%, Dif 73.67% TITLE 3: X -436.057, Y 488.879, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 120 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01456 8200.00 8000.00 0.00 -0.01448 8400.00 8000.00 0.00 -0.01435 8600.00 8000.00 0.00 -0.01416 8800.00 8000.00 0.00 -0.01392 9000.00 8000.00 0.00 -0.01360 9200.00 8000.00 0.00 -0.01324 9400.00 8000.00 0.00 -0.01287 9600.00 8000.00 0.00 -0.01252 9800.00 8000.00 0.00 -0.01216 10000.00 8000.00 0.00 -0.01180 10200.00 8000.00 0.00 -0.01141 10400.00 8000.00 0.00 -0.01097 10600.00 8000.00 0.00 -0.01050 10800.00 8000.00 0.00 -0.01002 11000.00 8000.00 0.00 -0.00950 11200.00 8000.00 0.00 -0.00891 11400.00 8000.00 0.00 -0.00820 11600.00 8000.00 0.00 -0.00734 11800.00 8000.00 0.00 -0.00635 12000.00 8000.00 0.00 -0.00530 12200.00 8000.00 0.00 -0.00422 12400.00 8000.00 0.00 -0.00314 12600.00 8000.00 0.00 -0.00201 12800.00 8000.00 0.00 -0.00078 13000.00 8000.00 0.00 0.00062 13200.00 8000.00 0.00 0.00218 13400.00 8000.00 0.00 0.00390 13600.00 8000.00 0.00 0.00571 13800.00 8000.00 0.00 0.00758 14000.00 8000.00 0.00 0.00947 14200.00 8000.00 0.00 0.01139 14400.00 8000.00 0.00 0.01334 14600.00 8000.00 0.00 0.01533 14800.00 8000.00 0.00 0.01742 15000.00 8000.00 0.00 0.01965 15200.00 8000.00 0.00 0.02203 15400.00 8000.00 0.00 0.02451 15600.00 8000.00 0.00 0.02701 15800.00 8000.00 0.00 0.02943 16000.00 8000.00 0.00 0.03174 16200.00 8000.00 0.00 0.03399 16400.00 8000.00 0.00 0.03629 16600.00 8000.00 0.00 0.03873 16800.00 8000.00 0.00 0.04135 17000.00 8000.00 0.00 0.04416 17200.00 8000.00 0.00 0.04712 17400.00 8000.00 0.00 0.05018 17600.00 8000.00 0.00 0.05338 17800.00 8000.00 0.00 0.05669 18000.00 8000.00 0.00 0.06004 18200.00 8000.00 0.00 0.06339 18400.00 8000.00 0.00 0.06667 18600.00 8000.00 0.00 0.06991 18800.00 8000.00 0.00 0.07324 19000.00 8000.00 0.00 0.07671 19200.00 8000.00 0.00 0.08024 19400.00 8000.00 0.00 0.08357 19600.00 8000.00 0.00 0.08638 19800.00 8000.00 0.00 0.08840 20000.00 8000.00 0.00 0.08961 20200.00 8000.00 0.00 0.09024 20400.00 8000.00 0.00 0.09053 20600.00 8000.00 0.00 0.09075 20800.00 8000.00 0.00 0.09096 21000.00 8000.00 0.00 0.09106 20400.00 8200.00 0.00 0.09117 20600.00 8200.00 0.00 0.09139 20800.00 8200.00 0.00 0.09158 21000.00 8200.00 0.00 0.09165 20400.00 8400.00 0.00 0.09188 20600.00 8400.00 0.00 0.09209 20800.00 8400.00 0.00 0.09226 21000.00 8400.00 0.00 0.09229 20200.00 8600.00 0.00 0.09261 20400.00 8600.00 0.00 0.09291 20600.00 8600.00 0.00 0.09309 20800.00 8600.00 0.00 0.09323 20000.00 8800.00 0.00 0.09330 20200.00 8800.00 0.00 0.09395 20400.00 8800.00 0.00 0.09422 20600.00 8800.00 0.00 0.09438 20800.00 8800.00 0.00 0.09447 20000.00 9000.00 0.00 0.09479 20200.00 9000.00 0.00 0.09541 20400.00 9000.00 0.00 0.09566 20600.00 9000.00 0.00 0.09578 20800.00 9000.00 0.00 0.09584 20000.00 9200.00 0.00 0.09627 20200.00 9200.00 0.00 0.09688 20400.00 9200.00 0.00 0.09710 20600.00 9200.00 0.00 0.09719 20800.00 9200.00 0.00 0.09721 20000.00 9400.00 0.00 0.09763 20200.00 9400.00 0.00 0.09822 20400.00 9400.00 0.00 0.09842 20600.00 9400.00 0.00 0.09847 20000.00 9600.00 0.00 0.09888 20200.00 9600.00 0.00 0.09945 20400.00 9600.00 0.00 0.09961 20600.00 9600.00 0.00 0.09963 20000.00 9800.00 0.00 0.10021 20200.00 9800.00 0.00 0.10075 20400.00 9800.00 0.00 0.10089 20000.00 10000.00 0.00 0.10177 20200.00 10000.00 0.00 0.10227 20400.00 10000.00 0.00 0.10237 20000.00 10200.00 0.00 0.10348 20200.00 10200.00 0.00 0.10394 20400.00 10200.00 0.00 0.10399 19800.00 10400.00 0.00 0.10410 20000.00 10400.00 0.00 0.10526 20200.00 10400.00 0.00 0.10568 20400.00 10400.00 0.00 0.10569 19800.00 10600.00 0.00 0.10590 20000.00 10600.00 0.00 0.10702 20200.00 10600.00 0.00 0.10740 19800.00 10800.00 0.00 0.10761 20000.00 10800.00 0.00 0.10869 20200.00 10800.00 0.00 0.10903 19800.00 11000.00 0.00 0.10933 20000.00 11000.00 0.00 0.11037 20200.00 11000.00 0.00 0.11067 19800.00 11200.00 0.00 0.11117 20000.00 11200.00 0.00 0.11218 20200.00 11200.00 0.00 0.11244 19800.00 11400.00 0.00 0.11311 20000.00 11400.00 0.00 0.11408 20200.00 11400.00 0.00 0.11432 19800.00 11600.00 0.00 0.11506 20000.00 11600.00 0.00 0.11601 20200.00 11600.00 0.00 0.11623 19800.00 11800.00 0.00 0.11701 20000.00 11800.00 0.00 0.11794 20200.00 11800.00 0.00 0.11815 19800.00 12000.00 0.00 0.11889 20000.00 12000.00 0.00 0.11982 20200.00 12000.00 0.00 0.12001 19800.00 12200.00 0.00 0.12079 20000.00 12200.00 0.00 0.12171 20200.00 12200.00 0.00 0.12189 19800.00 12400.00 0.00 0.12291 20000.00 12400.00 0.00 0.12383 20200.00 12400.00 0.00 0.12399 19800.00 12600.00 0.00 0.12525 20000.00 12600.00 0.00 0.12616 20200.00 12600.00 0.00 0.12631 19800.00 12800.00 0.00 0.12770 20000.00 12800.00 0.00 0.12860 20200.00 12800.00 0.00 0.12872 19800.00 13000.00 0.00 0.13020 20000.00 13000.00 0.00 0.13107 20200.00 13000.00 0.00 0.13114 19800.00 13200.00 0.00 0.13265 20000.00 13200.00 0.00 0.13347 20200.00 13200.00 0.00 0.13347 19800.00 13400.00 0.00 0.13501 20000.00 13400.00 0.00 0.13575 19800.00 13600.00 0.00 0.13747 20000.00 13600.00 0.00 0.13813 19600.00 13800.00 0.00 0.13833 19800.00 13800.00 0.00 0.14014 20000.00 13800.00 0.00 0.14073 19600.00 14000.00 0.00 0.14119 19800.00 14000.00 0.00 0.14295 20000.00 14000.00 0.00 0.14347 19600.00 14200.00 0.00 0.14411 19800.00 14200.00 0.00 0.14581 20000.00 14200.00 0.00 0.14627 19600.00 14400.00 0.00 0.14702 19800.00 14400.00 0.00 0.14865 20000.00 14400.00 0.00 0.14904 19600.00 14600.00 0.00 0.14977 19800.00 14600.00 0.00 0.15134 20000.00 14600.00 0.00 0.15166 19600.00 14800.00 0.00 0.15244 19800.00 14800.00 0.00 0.15395 20000.00 14800.00 0.00 0.15419 19600.00 15000.00 0.00 0.15518 19800.00 15000.00 0.00 0.15661 20000.00 15000.00 0.00 0.15677 19600.00 15200.00 0.00 0.15785 19800.00 15200.00 0.00 0.15922 20000.00 15200.00 0.00 0.15928 19600.00 15400.00 0.00 0.16035 19800.00 15400.00 0.00 0.16164 19600.00 15600.00 0.00 0.16271 19800.00 15600.00 0.00 0.16394 19600.00 15800.00 0.00 0.16498 19800.00 15800.00 0.00 0.16615 19600.00 16000.00 0.00 0.16732 19800.00 16000.00 0.00 0.16843 19600.00 16200.00 0.00 0.17005 19800.00 16200.00 0.00 0.17109 19600.00 16400.00 0.00 0.17319 19800.00 16400.00 0.00 0.17415 19600.00 16600.00 0.00 0.17648 19800.00 16600.00 0.00 0.17733 19600.00 16800.00 0.00 0.17969 19800.00 16800.00 0.00 0.18041 19600.00 17000.00 0.00 0.18265 19800.00 17000.00 0.00 0.18323 19600.00 17200.00 0.00 0.18529 19800.00 17200.00 0.00 0.18573 19400.00 17400.00 0.00 0.18577 19600.00 17400.00 0.00 0.18786 19800.00 17400.00 0.00 0.18815 19400.00 17600.00 0.00 0.18859 19600.00 17600.00 0.00 0.19056 19800.00 17600.00 0.00 0.19071 19400.00 17800.00 0.00 0.19137 19600.00 17800.00 0.00 0.19323 19400.00 18000.00 0.00 0.19393 19600.00 18000.00 0.00 0.19567 19400.00 18200.00 0.00 0.19619 19600.00 18200.00 0.00 0.19780 19400.00 18400.00 0.00 0.19802 19600.00 18400.00 0.00 0.19952 19400.00 18600.00 0.00 0.19974 19600.00 18600.00 0.00 0.20111 19400.00 18800.00 0.00 0.20184 19600.00 18800.00 0.00 0.20303 19400.00 19000.00 0.00 0.20419 19600.00 19000.00 0.00 0.20511 19400.00 19200.00 0.00 0.20633 19600.00 19200.00 0.00 0.20692 19400.00 19400.00 0.00 0.20797 19600.00 19400.00 0.00 0.20816 19400.00 19600.00 0.00 0.20868 19400.00 19600.00 5.00 0.20873 19400.00 19600.00 10.00 0.20877 19400.00 19600.00 15.00 0.20881 19400.00 19600.00 20.00 0.20883 19400.00 19600.00 25.00 0.20884 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19455.45 19600.00 20.55 0.20891 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/471.ctf NX, NY, NZ: 360 360 1 MODE : real