CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs/472.mrc Output diagnostic file name Micrographs/472.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 360 35.0 5.0 8000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs/472.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2192.000 8070.000 Mean, RMS : 4331.722 211.8738 TITLE 1: emi2mrc: 472.emi Mon Jan 20 12:41:52 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.05%, Dif 73.67% TITLE 3: X -434.451, Y 490.879, Z -26.366, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 121 119 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 8000.00 8000.00 0.00 -0.01573 8200.00 8000.00 0.00 -0.01552 8400.00 8000.00 0.00 -0.01525 8600.00 8000.00 0.00 -0.01497 8800.00 8000.00 0.00 -0.01471 9000.00 8000.00 0.00 -0.01444 9200.00 8000.00 0.00 -0.01414 9400.00 8000.00 0.00 -0.01383 9600.00 8000.00 0.00 -0.01350 9800.00 8000.00 0.00 -0.01312 10000.00 8000.00 0.00 -0.01270 10200.00 8000.00 0.00 -0.01218 10400.00 8000.00 0.00 -0.01156 10600.00 8000.00 0.00 -0.01084 10800.00 8000.00 0.00 -0.01008 11000.00 8000.00 0.00 -0.00929 11200.00 8000.00 0.00 -0.00847 11400.00 8000.00 0.00 -0.00758 11600.00 8000.00 0.00 -0.00659 11800.00 8000.00 0.00 -0.00549 12000.00 8000.00 0.00 -0.00431 12200.00 8000.00 0.00 -0.00312 12400.00 8000.00 0.00 -0.00196 12600.00 8000.00 0.00 -0.00086 12800.00 8000.00 0.00 0.00022 13000.00 8000.00 0.00 0.00133 13200.00 8000.00 0.00 0.00253 13400.00 8000.00 0.00 0.00387 13600.00 8000.00 0.00 0.00539 13800.00 8000.00 0.00 0.00708 14000.00 8000.00 0.00 0.00892 14200.00 8000.00 0.00 0.01084 14400.00 8000.00 0.00 0.01278 14600.00 8000.00 0.00 0.01472 14800.00 8000.00 0.00 0.01665 15000.00 8000.00 0.00 0.01863 15200.00 8000.00 0.00 0.02073 15400.00 8000.00 0.00 0.02295 15600.00 8000.00 0.00 0.02522 15800.00 8000.00 0.00 0.02748 16000.00 8000.00 0.00 0.02968 16200.00 8000.00 0.00 0.03188 16400.00 8000.00 0.00 0.03417 16600.00 8000.00 0.00 0.03662 16800.00 8000.00 0.00 0.03924 17000.00 8000.00 0.00 0.04199 17200.00 8000.00 0.00 0.04478 17400.00 8000.00 0.00 0.04761 17600.00 8000.00 0.00 0.05054 17800.00 8000.00 0.00 0.05359 18000.00 8000.00 0.00 0.05675 18200.00 8000.00 0.00 0.05992 18400.00 8000.00 0.00 0.06298 18600.00 8000.00 0.00 0.06589 18800.00 8000.00 0.00 0.06878 19000.00 8000.00 0.00 0.07179 19200.00 8000.00 0.00 0.07502 19400.00 8000.00 0.00 0.07839 19600.00 8000.00 0.00 0.08167 19800.00 8000.00 0.00 0.08455 20000.00 8000.00 0.00 0.08685 20200.00 8000.00 0.00 0.08855 20400.00 8000.00 0.00 0.08970 20600.00 8000.00 0.00 0.09044 20800.00 8000.00 0.00 0.09083 21000.00 8000.00 0.00 0.09084 20400.00 8200.00 0.00 0.09095 20600.00 8200.00 0.00 0.09167 20800.00 8200.00 0.00 0.09203 20400.00 8400.00 0.00 0.09214 20600.00 8400.00 0.00 0.09282 20800.00 8400.00 0.00 0.09314 20400.00 8600.00 0.00 0.09337 20600.00 8600.00 0.00 0.09399 20800.00 8600.00 0.00 0.09427 20400.00 8800.00 0.00 0.09460 20600.00 8800.00 0.00 0.09518 20800.00 8800.00 0.00 0.09541 20400.00 9000.00 0.00 0.09578 20600.00 9000.00 0.00 0.09630 20800.00 9000.00 0.00 0.09650 20400.00 9200.00 0.00 0.09691 20600.00 9200.00 0.00 0.09740 20800.00 9200.00 0.00 0.09758 20400.00 9400.00 0.00 0.09801 20600.00 9400.00 0.00 0.09847 20800.00 9400.00 0.00 0.09865 20400.00 9600.00 0.00 0.09907 20600.00 9600.00 0.00 0.09952 20800.00 9600.00 0.00 0.09970 20400.00 9800.00 0.00 0.10022 20600.00 9800.00 0.00 0.10067 20800.00 9800.00 0.00 0.10086 20400.00 10000.00 0.00 0.10148 20600.00 10000.00 0.00 0.10193 20800.00 10000.00 0.00 0.10214 20400.00 10200.00 0.00 0.10269 20600.00 10200.00 0.00 0.10317 20800.00 10200.00 0.00 0.10339 20400.00 10400.00 0.00 0.10380 20600.00 10400.00 0.00 0.10431 20800.00 10400.00 0.00 0.10455 20400.00 10600.00 0.00 0.10481 20600.00 10600.00 0.00 0.10535 20800.00 10600.00 0.00 0.10561 20400.00 10800.00 0.00 0.10577 20600.00 10800.00 0.00 0.10634 20800.00 10800.00 0.00 0.10662 20400.00 11000.00 0.00 0.10689 20600.00 11000.00 0.00 0.10749 20800.00 11000.00 0.00 0.10779 20400.00 11200.00 0.00 0.10838 20600.00 11200.00 0.00 0.10901 20800.00 11200.00 0.00 0.10931 20400.00 11400.00 0.00 0.11020 20600.00 11400.00 0.00 0.11085 20800.00 11400.00 0.00 0.11115 20200.00 11600.00 0.00 0.11117 20400.00 11600.00 0.00 0.11227 20600.00 11600.00 0.00 0.11292 20800.00 11600.00 0.00 0.11319 20200.00 11800.00 0.00 0.11335 20400.00 11800.00 0.00 0.11445 20600.00 11800.00 0.00 0.11507 20800.00 11800.00 0.00 0.11530 20200.00 12000.00 0.00 0.11553 20400.00 12000.00 0.00 0.11661 20600.00 12000.00 0.00 0.11720 20800.00 12000.00 0.00 0.11737 20200.00 12200.00 0.00 0.11769 20400.00 12200.00 0.00 0.11874 20600.00 12200.00 0.00 0.11927 20800.00 12200.00 0.00 0.11939 20200.00 12400.00 0.00 0.11992 20400.00 12400.00 0.00 0.12093 20600.00 12400.00 0.00 0.12142 20800.00 12400.00 0.00 0.12148 20200.00 12600.00 0.00 0.12219 20400.00 12600.00 0.00 0.12317 20600.00 12600.00 0.00 0.12361 20800.00 12600.00 0.00 0.12361 20200.00 12800.00 0.00 0.12441 20400.00 12800.00 0.00 0.12535 20600.00 12800.00 0.00 0.12574 20200.00 13000.00 0.00 0.12654 20400.00 13000.00 0.00 0.12746 20600.00 13000.00 0.00 0.12780 20200.00 13200.00 0.00 0.12859 20400.00 13200.00 0.00 0.12947 20600.00 13200.00 0.00 0.12976 20200.00 13400.00 0.00 0.13061 20400.00 13400.00 0.00 0.13145 20600.00 13400.00 0.00 0.13170 20200.00 13600.00 0.00 0.13282 20400.00 13600.00 0.00 0.13363 20600.00 13600.00 0.00 0.13384 20200.00 13800.00 0.00 0.13537 20400.00 13800.00 0.00 0.13614 20600.00 13800.00 0.00 0.13629 20000.00 14000.00 0.00 0.13660 20200.00 14000.00 0.00 0.13812 20400.00 14000.00 0.00 0.13884 20600.00 14000.00 0.00 0.13892 20000.00 14200.00 0.00 0.13950 20200.00 14200.00 0.00 0.14097 20400.00 14200.00 0.00 0.14161 20600.00 14200.00 0.00 0.14161 20000.00 14400.00 0.00 0.14241 20200.00 14400.00 0.00 0.14381 20400.00 14400.00 0.00 0.14437 20000.00 14600.00 0.00 0.14519 20200.00 14600.00 0.00 0.14652 20400.00 14600.00 0.00 0.14699 20000.00 14800.00 0.00 0.14790 20200.00 14800.00 0.00 0.14916 20400.00 14800.00 0.00 0.14957 20000.00 15000.00 0.00 0.15068 20200.00 15000.00 0.00 0.15188 20400.00 15000.00 0.00 0.15221 20000.00 15200.00 0.00 0.15342 20200.00 15200.00 0.00 0.15456 20400.00 15200.00 0.00 0.15482 20000.00 15400.00 0.00 0.15600 20200.00 15400.00 0.00 0.15709 20400.00 15400.00 0.00 0.15731 20000.00 15600.00 0.00 0.15849 20200.00 15600.00 0.00 0.15954 20400.00 15600.00 0.00 0.15971 20000.00 15800.00 0.00 0.16091 20200.00 15800.00 0.00 0.16191 20400.00 15800.00 0.00 0.16202 20000.00 16000.00 0.00 0.16335 20200.00 16000.00 0.00 0.16430 20400.00 16000.00 0.00 0.16433 20000.00 16200.00 0.00 0.16606 20200.00 16200.00 0.00 0.16693 19800.00 16400.00 0.00 0.16708 20000.00 16400.00 0.00 0.16899 20200.00 16400.00 0.00 0.16975 19800.00 16600.00 0.00 0.17003 20000.00 16600.00 0.00 0.17183 20200.00 16600.00 0.00 0.17246 19800.00 16800.00 0.00 0.17273 20000.00 16800.00 0.00 0.17441 20200.00 16800.00 0.00 0.17491 19800.00 17000.00 0.00 0.17512 20000.00 17000.00 0.00 0.17669 20200.00 17000.00 0.00 0.17706 19800.00 17200.00 0.00 0.17727 20000.00 17200.00 0.00 0.17874 20200.00 17200.00 0.00 0.17900 19800.00 17400.00 0.00 0.17954 20000.00 17400.00 0.00 0.18090 20200.00 17400.00 0.00 0.18105 19800.00 17600.00 0.00 0.18217 20000.00 17600.00 0.00 0.18342 20200.00 17600.00 0.00 0.18344 19800.00 17800.00 0.00 0.18494 20000.00 17800.00 0.00 0.18608 19800.00 18000.00 0.00 0.18760 20000.00 18000.00 0.00 0.18860 19800.00 18200.00 0.00 0.18997 20000.00 18200.00 0.00 0.19083 19800.00 18400.00 0.00 0.19192 20000.00 18400.00 0.00 0.19266 19800.00 18600.00 0.00 0.19375 20000.00 18600.00 0.00 0.19435 19800.00 18800.00 0.00 0.19594 20000.00 18800.00 0.00 0.19632 19600.00 19000.00 0.00 0.19652 19800.00 19000.00 0.00 0.19840 20000.00 19000.00 0.00 0.19848 19600.00 19200.00 0.00 0.19917 19800.00 19200.00 0.00 0.20072 19600.00 19400.00 0.00 0.20145 19800.00 19400.00 0.00 0.20256 19600.00 19600.00 0.00 0.20280 19800.00 19600.00 0.00 0.20341 19600.00 19800.00 40.00 0.20342 19600.00 19800.00 45.00 0.20347 19600.00 19800.00 50.00 0.20352 19600.00 19800.00 55.00 0.20354 19600.00 19800.00 60.00 0.20356 19600.00 19800.00 65.00 0.20356 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19615.77 19775.41 65.00 0.20357 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs/472.ctf NX, NY, NZ: 360 360 1 MODE : real