DETERMINATION OF DEFOCUS GRADIENT DIRECTION, V1.O (Aug-2009) ============================================================ Input tilt-series file name: /gpfs/appion/anoble/17jul06c/protomo_alignments/tiltseries0004_thick200/defocus_estimation/stack_for_defocus_estimation.mrcs Input tilt-angles file name: /gpfs/appion/anoble/17jul06c/protomo_alignments/tiltseries0004_thick200/defocus_estimation/series0004_tilts.txt Output diagnostic file name: /gpfs/appion/anoble/17jul06c/protomo_alignments/tiltseries0004_thick200/defocus_estimation//diagnostic.mrc Tile size: 384 Input CTF parameters: CS[mm], HT[kV], AmpCnst, XMAG, DStep[um]: 0.0, 300.0, 0.07, 10000.0, 1.8 Angstroms/Pixel: 1.84 Input Fitting parameters: CTFMin[0,1], ResMin[A], ResMax[A]: 0.5, 100000.00, 7.3 dFMin[A], dFMax[A], FStep: 48000.0, 72000.00, 500.0 Summary of defocus gradients: ============================= Image TiltAngle DF-left* DF-center* DF-right* ------------------------------------------------------------- 0 -39.0 72000.0 72000.0 49500.0 1 -36.0 66000.0 50000.0 67500.0 2 -33.0 48500.0 71000.0 72000.0 3 -30.0 51000.0 70000.0 68500.0 4 -27.0 60500.0 72000.0 71500.0 5 -24.0 62500.0 72000.0 71000.0 6 -21.0 63000.0 70000.0 62000.0 7 -18.0 68000.0 72000.0 71500.0 8 -15.0 62000.0 72000.0 70000.0 9 -12.0 49000.0 56000.0 57000.0 10 -9.0 62000.0 48500.0 72000.0 11 -6.0 70500.0 71000.0 67500.0 12 -3.0 51500.0 71500.0 70000.0 13 -0.1 50000.0 51500.0 68500.0 14 0.1 72000.0 71000.0 70000.0 15 3.0 66000.0 64500.0 72000.0 16 6.0 71000.0 72000.0 65500.0 17 9.0 62000.0 72000.0 63500.0 18 12.0 71500.0 72000.0 72000.0 19 15.0 49000.0 62000.0 48500.0 20 18.0 72000.0 72000.0 71000.0 21 21.0 63500.0 72000.0 72000.0 22 24.0 68000.0 71500.0 71500.0 23 27.0 67000.0 69000.0 52000.0 24 30.0 67000.0 68000.0 65500.0 25 33.0 64500.0 67000.0 66500.0 26 36.0 68000.0 66500.0 63500.0 27 39.0 64000.0 67000.0 68000.0 28 42.0 63500.0 67500.0 70000.0 ------------------------------------------------------------- * Approx. DF values corresponding to the X columns: left center right 384 1920 3456 ------------------------------------------------------------- Tilt angles NOT according to the convention used in TOMOCTF: Please, change the sign of tilt angles to follow the convention: Images with positive tilt angle must have increasing underfocus magnitude with the X axis (left to right)